• Journal of Nutrition and Health
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• 제36권6호
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• pp.646-652
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• 2003

This study was performed to determine the iron status of the adolescent Korean girls before and after menarche. The 101 subjects aged 11-13 years who attending in an elementary school in Mokpo were recruited. They were divided into pre-menarche (A) group or post-menarche (B) group based on their menstruation status. The latter subjects were sub-divided into one of the four groups according to the times of their menstruation B-I( $\geq$ 3 times), B-II (4-6 times), B-III (7-9 times) or B-W ( $\geq$ 10 times). In the total subjects, dietary iron intake, 11.3 mg/day, was below the Korean RDA for iron, the percentage of heme iron to total iron intake, 15%, and the bioavailability of dietary iron, 12.3%, seemed to be low. And their body iron storage, 140.8 mg, seemed to be insufficient. However, they tended to meet body's iron requirement in the cell level. Red blood cell number (RBC), hematocrit (Hct), and hemoglobin (Hb) level in the total subjects were 4.5 1012/I, 39.3%, and 13.0 g/㎗, respectively. The subjects in B group had lower (p<0.05) RBC and Hct compared to those in A group and the prevalence of iron-deficiency anemia tended to be high. Serum iron, ferritin, and soluble transferrin receptor (sTfR) and sTfR:ferritin ratio were 86.7 $\mu\textrm{g}$/d, 17.6 $\mu\textrm{g}$/l, 3.58 mg/1, and 230, respectively. Those four indices were not significantly different among the groups. The results of this study imply that, although there a tendency to affect negatively iron status, menstrual blood loss in adolescent females does not deteriorate obviously their iron status during the relatively short period up to 1 you. However, it should be better to improve their iron status after starting menarche by increasing iron intake, especially heme-iron, and enhancing factors for iron absorption.

• 동의생리병리학회지
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• 제25권5호
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• pp.857-862
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• 2011

The purpose of this study is to investigate the anti-proliferative mechanism by Trichosanthes kirilowii (TCK) in MCF-7 human breast carcinoma cell. In this study, we used human breast cancer cell line, Michigan cancer foundation-7 cells (MCF-7 cells). They were co-incubated with 30~200 ${\mu}g$/ml TCK for 48 hours, and cell viability was measured by Water-soluble tetrazolium salt-1 (WST-1) assay. After MCF-7 cells were exposed to 60 ${\mu}g$/ml of TCK for 0, 3, 6, 12, 24, 48 hours, We performed flow analysis cytometry sorting(FACS) and western blot analysis. We investigated the effect of dose-dependent cell growth inhibition by TCK, which could be proved by WST-1 assay. Also, flow cytometry analysis showed that TCK increased percentage of subG1 phase and G2/M phase cell cycle. In addition, TCK induced apoptosis through the expression of caspase-9, -3 and poly(ADP-ribose) polymerase(PARP) activation. Moreover, we showed that ATM-dependent G2/M phase arrest by DNA damage and phosphorylation of chk2, cdc25C, cdc2(Tyr15). Taken together, these results suggest that by G2/M phase arrest through DNA damage and inducing of apoptosis through intrinsic pathway, TCK may have potential tumor suppressor in breast cancer.

• 동의생리병리학회지
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• 제25권5호
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• pp.863-869
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• 2011

The aim of the present study is to investigate whether an ethanol extract of Acorus gramineus Soland (EAG) augments penile erection in vitro and in vivo experiment. Preconstructed with phenylephrine (PE) in isolated endothelium-intact rabbit corpus cavernosum, EAG relaxed penile smooth muscle in a dose-dependent manner, which was inhibited by pretreatment with NG-nitro-L-argininemethylester (L-NAME), a nitricoxide synthase inhibitor, and 1H-[1,2,4]-oxadiazole-[4,3-${\alpha}$]-quinoxalin-1-one (ODQ), a soluble guanylylcyclase (sGC) inhibitor, respectively. EAG-induced relaxation was significantly attenuated by pretreatment with tetraethylammonium (TEA), a nonselective $K^+$ channel blocker. EAG increased cGMP levels of the rabbit corpus cavernosum in a concentration-dependent manner without changes in cAMP levels. In addition, EAG caused increase of peak intracavernous pressure (ICP), ICP/MAP ratio and area under the carve (AUC) in SD rats. Taken together, these results suggest that EAG augments penile erection via NO-cGMP system and $K^+$ channels in corpus cavernosum.

• 대한한방부인과학회지
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• 제21권1호
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• pp.28-38
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• 2008

Purpose: This study was conducted to investigate the muscular strength & stamina improvement and muscle anti-fatigue effects of Morinda officinalis How after long term administration. Methods: 4-6 weeks old ICR mice were used in this study and we administered the water soluble extracts of Morinda officinalis How in the concentration of 1, 10. 100/mg/0.3ml to each 5 mice (ATS group) and same volume of normal saline was administered to 5 mice (control group) once a day for 90 or 120 days. After the administration we performed the swimming exercise test and the grip strength test. And we measured the concentration of glucose and the activity of lactate dehydrogenase in plasma regarded as biochemical indicator related with the muscular fatigue. Results: In swimming exercise test, all ATS groups showed increased time compared with relative control group after 90 days administration. After 120 days adminstration 100/mg/0.3ml ATS groups showed increased results. In grip strength test, all ATS groups showed increased grip strength compared with relative control group after 90 days administration. After 120 days adminstration 10/mg/0.3ml ATS groups showed increased result. All the results of plasma glucose didn't showed statistically significant difference after 90 and 120 days administration. In the plasma concentration of activity level of lactate dehydrogenase, all ATS groups didn't showed statistically significant difference compared after 90 days administration. Conclusion: This study show that Morinda officinalis How can increase the muscular strength & stamina and anti-fatigue effect.

• Environmental Engineering Research
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• 제23권2호
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• pp.205-209
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• 2018

The spectral composition of light can affect the growth and biochemical composition of photosynthetic microalgae. This study examined the use of light filtering through a solution of soluble colored additives, a cost-effective method to alter the light spectrum, on the growth and lipid production of an oleaginous microalga, Nannochloropsis gaditana (N. gaditana). Cells were photoautotrophically cultivated under a white light emitting diode (LED) alone (control) or under a white LED that passed through a solution of red and yellow color additive (4:1 ratio) that blocked light below 600 nm. The specific growth rate was significantly greater under filtered light than white light ($0.2672d^{-1}$ vs. $0.1930d^{-1}$). Growth under filtered light also increased the fatty acid methyl ester (FAME) yield by 22.4% and FAME productivity by 80.0%, relative to the white light control. In addition, the content of saturated fatty acids was greater under filtered light, so the biodiesel products had better stability. These results show that passing white light through an inexpensive color filter can simultaneously enhance cellular growth and lipid productivity of N. gaditana. This approach of optimizing the light spectrum may be applicable to other species of microalgae.

• 대한한방부인과학회지
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• 제20권2호
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• pp.60-70
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• 2007

Purpose: This study was conducted to investigate the muscle anti-fatigue effects of Allii tuberosi semen after short term administration. Methods: 4-6 weeks old ICR mice were used in this study and we administered the water soluble extracts of Allii tuberosi semen in the concentration of 1, 10 and 100mg/0.3ml to each 5 mice (ATS group) and same volume of normal saline was administered to 5 mice (control group) once a day for 30 or 60 days. After the administration we performed the swimming exercise test and the grip strength test. And we measured the concentration of glucose and the activity of lactate dehydrogenase in plasma regarded as biochemical indicator related with the muscular fatigue.. Results: In swimming exercise test. 10 and 100mg/0.3ml ATS groups showed increased time compared with relative control group after 30 days administration. But after 60 days adminstration all ATS groups showed increased results. In grip strength test, 100mg/0.3ml ATS group increased grip strength after 30days administration and 10mg/0.3ml ATS group showed increased result after 60days administration. All the results of plasma glucose and lactate dehydrogenase didn't showed statistically significant difference after 30 and 60 days administration. Conclusion: From this study we could find the anti-fatigue effect of Allii tuberosi semen in the muscle strength related test.

• 생물환경조절학회지
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• 제19권4호
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• pp.284-289
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• 2010

플라스틱하우스 피복재의 광 이용효율을 높이기 위해 제조과정에 적색, 청색, 적색+청색 수지를 추가로 첨가하여 제조한 필름을 피복한 시설 내에서 토마토와 고추를 재배하였으며 시설 내 광 환경 변화와 작물의 생육, 수량 등을 조사하였다. 대체로 일반필름에 비하여 적색 및 청색 수지를 첨가한 필름에서 광합성유효복사의 투과량이 5~6% 많았고, 300~1,100nm의 광투과율은 0.5~1.0% 높게 나타났다. 피복재 중에는 적색+청색 혼합수지 첨가필름에서 토마토와 고추의 건물중이 높게 나타났고 일반필름에 비해 과실수량이 15~20% 많았으며 과실 당도도 약 $0.5^{\circ}Bx$. 높았다.

• 공업화학
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• 제8권3호
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• pp.365-373
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• 1997

천연 고분자인 키토산의 1차 아민기를 아질산 나트륨에 의해 oxidative-deamination 반응을 일으켜 reducing end에 알데히드기를 갖는 키토산 올리고머를 얻었으며, 이를 수소화붕소산나트륨으로 환원하여 2,5-anhydro-D-mannitol로 전환하였다. 얻어진 피토산 올리고머의 평균 중합도는 겔 투과 크로마토그래피를 통해 2정도임을 알 수 있었고, 키토산에 비해 친유성 유기 용매에 대한 높은 용해성을 나타내었다. 키토산 올리고머에 아실클로라이드를 4-디메틸아미노피리딘 촉매하에서 반응시켜 N,N-디아실, O-아실 키토산 올리고머를 합성하였다. 시차주사열량 분석을 실시한 결과 N,N-디라우로일, O-라우로일 키토산 올리고머의 경우 액정 영역을 나타내었고, 편광현미경을 통해 이를 확인할 수 있었다. X-선 회절분석 실험결과 키토산 올리고머와 달리 N,N-디라우로일, O-라우로일 키토산 올리고머는 높은 결정성 구조를 나타냄을 알 수 있었다.

• 대한화학회지
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• 제30권2호
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• pp.201-206
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• 1986

새로운 분석시약 N-benzylisonitrosoacetylacetone imine (H-IAA-N-Bz)을 합성하여 원소분석, 적외선흡수스펙트럼, 핵자기공명흡수스펙트럼, 질량스펙트럼 등으로 그 구조를 확인하였다. H-IAA-N-Bz은 알카리 용액(pH = 7.0∼10.0)에서 구리착물을 형성하며, 클로로포름 유기용매층으로 잘 추출된다. 이 구리착물에 대한 분광광도법적 연구의 여러가지의 최적조건은 420nm의 파장에서 구하였으며, 클로로포름 10ml당 구리 $\mu$g이하의 농도 범위에서 Beer법칙이 잘 성립되었다. 구리 착물의 조성은 $Cu(IAA-N-Bz)_2$이며, 착물의 총괄 안정도상수는 $8.55{\times}10^6$이며, 몰흡광계수 $\varepsilon$은 3500l/$cm{\cdot}mol$이다.

• 한국식품조리과학회지
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• 제18권2호
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• pp.200-205
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• 2002

본 연구는 옥수수 전분의 기능 특성을 개선시키고자, 다양한 sodium hypochlorite(NaOCl)농도에서 산화시킨 산화 전분의 분자 구조적 변화에 대하여 시험하였다. 전분의 분자량 분포에서 산화 전분은 고분자량의 피크가 점차 작아지고 저분자량 부분의 피크가 높아져 전체적 분자량 분포가 낮은 쪽으로 이동하였고, 요오드 최대 흡수 파장이 감소되면서 색깔이 변화하였다. 무처리 전분은 온도가 증가함에 따라 분자량이 큰 부분의 용출이 증가하였다. 산화전분의 경우 아밀로펙틴이 산화에 의해 분해되어 전분 입자내에 큰 분자량을 가진 분자가 감소되면서 작은 분자량을 가지는 분자가 증가되기 때문에 가용성 탄수화물의 양이 증가하였다. DSC 특성에서는 상전이 온도 범위와 엔탈피의 차이는 없었으나 산화 전분이 무처리 전분보다 노화경향이 더 크게 나타났다. 산화전분용액의 광투과도는 증가하였다.