• Food Science and Preservation
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• v.9 no.2
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• pp.144-147
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• 2002

This study was conducted to investigate the effect of sub-ingredients of kimchi on the formation of carbon dioxide and vessel pressure. The pH, titratable acidity, formation of carbon dioxide and vessel pressure of kimchi prepared with different sub-ingredients were determined during storage at 10$^{\circ}C$. In the kimhi without GA, the pH decrease and acidity increase was slow during storage, but those of the kimchis without Gl, RP and SA were began to higher from 3th day after storage showed maximum values and was maintained 9th day after storage. The main sub-ingredient for formation of carbon dioxide was garlic and the gas formation was low in the kimchi without garlic. Vessel pressure in kimchi of the latter term of storage generally showed sub-atmospheric pressure and the more formation of carbon dioxide showed the more degree of sub-atmosphere.

• The Plant Pathology Journal
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• v.39 no.1
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• pp.75-87
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• 2023

The pine wood nematode (PWN), Bursaphelenchus xylophilus is a well-known devastating pathogen of economic importance in the Republic of Korea and other countries. In the Republic of Korea, trunk injection of nematicides is the preferred method of control. In this study, the efficacy of 16 locally produced formulations of emamectin benzoate against the PWN are compared through determining their sublethal toxicities and reproduction inhibition potentials. Nematodes were treated with varying concentrations of the tested chemicals in multi-well culture plates, and rates of paralysis and mortality were determined after 24 h. Reproduction inhibition potential was tested by inoculating pre-treated nematodes onto Botrytis cinerea, and in pine twig cuttings. Despite the uniformity in the concentration of the active ingredient, efficacy was contrastingly different among formulations. The formulations evidently conformed to three distinct groups based on similarities in sublethal activity (group 1: LC₉₅ of 0.00768-0.01443 mg/ ml; group 2: LC₉₅ of 0.03202-0.07236 mg/ml, and group 3: LC₉₅ of as high as 0.30643-0.40811 mg/ml). Nematode paralysis generally occurred at the application dose of 0.0134-0.1075 ㎍/ml, and there were significant differences in nematode paralysis rates among the products. Nematode reproduction was only evident at lower doses both on B. cinerea and pine twigs, albeit the variations among formulations. Group 1 formulations significantly reduced nematode reproduction even at a lower dose of 0.001075 ㎍/ml. The variations in efficacy might be attributed to differences in inert ingredients. Therefore, there is need to analyze the potential antagonistic effects of the large number of additives used in formulations.

• The Korean Journal of Pesticide Science
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• v.6 no.1
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• pp.31-35
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• 2002

Objectives of this study were to determine if impurities in technical grade benfuracarb inhibit glutathione-S-transferase and amidase and to identify structures of impurities in technical grade benfuracarb. Technical grade benfuracarb, active ingredient, and impurity inhibited glutathione-S-transferase, and their $I_{50}$ were $9.7{\times}10^{-4}M,\;>1.0{\times}10^{-3}M,\;1.8{\times}10^{-4}M$, respectively. Such inhibition, however, was not higher than that by ethacrynic acid, a selective inhibitor to GST. Technical grade benfuracarb, active ingredient, and impurity also inhibited amidase, and their $I_{50}$ were $6.0{\times}10^{-5}M,\;4.3{\times}10^{-4}M,\;7.6{\times}10^{-5}M$, respectively. Our results show that the inhibition of both detoxifying enzymes by impurities in benfuracarb was 10-fold lower than that by active ingredient, suggesting that both active ingredient and impurities are involved in the inhibition of both detoxifying enzymes. Of four impurities (IM $1{\sim}4$) that were separated from technical grade benfuracarb, IM 2 and IM 3 inhibited GST and amidase. Based on data from IR, $^1H$-NMR, $^{13}C$-NMR and MS, it was determined that IM 2 is ethyl-N-isopropylamino propionate and IM 3 is ethyl-N-isopropyl-N(chlorosulfenyl)aminopropionate.

• Journal of the Korean Society of Food Science and Nutrition
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• v.46 no.1
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• pp.146-151
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• 2017

Quality characteristics (moisture, protein, ash, bulk density, falling number, damaged kernel, and foreign material) of 443 domestic wheat samples harvested between 2011 and 2013 were compared with those of 160 imported wheat samples from the United States, Australia, and Canada. Moisture content of domestic wheat (10.9~13.9%) was generally higher than that of imported wheat (8.0~12.6%). Large variation in protein content was found in domestic wheat compared to imported wheat even though variation in protein content of domestic wheat tended to decrease every year, implying quality control efforts for domestic wheat. A similar trend was observed in ash content, which was approximately 0.1% higher in domestic wheat kernels over 3 years. Imported wheat samples had a falling number of 300 or above. On the other hand, some domestic wheat samples had a falling number of 300, which meant low quality of wheat kernels. Generally, quality variations in domestic wheat kernels decreased over the years; however, it is necessary to maintain minimum requirements of moisture content and falling number for high and consistent quality domestic wheat.

• Journal of the Korean Applied Science and Technology
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• v.38 no.6
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• pp.1466-1475
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• 2021

Compound K (20-O-β-(_D-glucopyranosyl)-20(S)-protopanaxadiol) is an active ingredient of ginsenosides. Compound K has been known to produce from biotransformation by β-glucosidase action of human intestinal microbes after oral admistration of ginseng. We have investigated the cytotoxicity of compound K obtained from bio-converted ginseng extract. As a result, compound K showed no significant cytotoxicity in the concentration of 0.001 to 1 ㎍/mL and inhibited the production of TNF-α, MCP-1, IL-6 and NO in RAW 264.7 cells induced by LPS inflamation. In the same concentration, HaCaT cells induced by inflammation with TNF-α and IFN-γ decreased IL-8 production due to compound K treatment. In the brine shrimp lethality assay, the LC₅₀ of compound K was 0.37 mg/mL indicating some toxicity, but the bioconverted product containing 35% compound K showed relatively low toxicity with an LC₅₀ of 0.87 mg/mL. These results suggest that the compound K enriched extract is a potential functional material for acne relief cosmetic products.

• Journal of the Korean Society of Food Culture
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• v.26 no.6
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• pp.574-582
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• 2011

The aim of this study was to classify Korean consumers based on their food-related lifestyle type, and to investigate the relationship between sushi consumption and food-related lifestyle type. Self-reported questionnaires were completed by 300 Korean adults. The SPSS 18.0 program was used to analyze the samples. Data was analyzed by frequency, descriptive factor, reliability, cluster analysis, ANOVA, and chi-square test. A factor analysis extracted four factors comprising foodrelated lifestyle, which we named Health-seeking (factor 1), Taste-seeking (factor 2), Convenience-seeking (factor 3), and Economy-seeking (factor 4). According to a cluster analysis based on those four factors, consumers were classified into three clusters. Cluster 1 was the Taste and Health-seeking cluster, Cluster 2 was the Convenience-seeking cluster, and Cluster 3 was the Passive Eating Habits cluster. The results also indicated that the selection attributes of each cluster were significantly different in terms of perception, the global state of sushi, sushi preference, frequency, companions, place of sushi consumption, and preference for different sushi sub-ingredients. Based on these results, consumer characteristics in the sushi market are discussed.

• Advances in concrete construction
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• v.9 no.4
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• pp.337-343
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• 2020

In the present study, by employing fragility analysis, the seismic vulnerability of a concrete girder bridge, one of the most common existing structural bridge systems, has been performed. To this end, drift demand model as a fundamental ingredient of any probabilistic decision-making analyses is initially developed in terms of the two most common intensity measures, i.e., PGA and Sa (T₁). Developing a probabilistic demand model requires a reliable database that is established in this paper by performing incremental dynamic analysis (IDA) under a set of 20 ground motion records. Next, by employing Bayesian statistical inference drift demand models are developed based on pre-collapse data obtained from IDA. Then, the accuracy and reasonability of the developed models are investigated by plotting diagnosis graphs. This graphical analysis demonstrates probabilistic demand model developed in terms of PGA is more reliable. Afterward, fragility curves according to PGA based-demand model are developed.

• Toxicological Research
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• v.31 no.1
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• pp.33-40
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• 2015

Bee venom (BV) has long been used in traditional Eastern and Western medicine for chronic inflammation, pain and skin therapy. Human exposure to BV, however, often causes unwanted adverse effects and is even fatal in some cases. Phospholipase $A_2$ ($PLA_2$) of BV is now suspected to play a key role in these adverse effects. We investigated the potential use of $PLA_2$-free bee venom (PBV) as a replacement for BV in cosmetic products. PBV prepared by molecular weight cut-off ultrafiltration exhibits a superior profile in comparison with regular BV, by inhibiting elastase activity and suppressing the induction of nitric oxide (NO) and metalloproteinase-9 (MMP-9), while retaining the effects of cell proliferation and protection against ultraviolet B (UVB)-induced damage in human dermal fibroblast cells. PBV thus appears to be more promising than BV as a cosmetic ingredient with a reduced potential for adverse reactions in the recipient.

• Journal of Powder Materials
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• v.11 no.2
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• pp.124-129
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• 2004

Nanosized WC and WC-Co powders were synthesised by chemical vapor condensation(CVC) process using the pyrolysis of tungsten hexacarbonyl(W(CO)$_6$) and cobalt octacarbonyl(Co$_2$(CO)$_8$). The microstructural changes and phase evolution of the CVC powders during post heat-treatment were studied using the XRD, FE-SEM, TEM, and ICP-MS. CVC powders were consisted of the loosely agglomerated sub-stoichimetric WC$_{1-x}$ and the long-chain Co nanopowders. The sub-stochiometric CVC WC and WC-Co powders were carburized using the mixture gas of CH$_4$-H$_2$ in the temperature range of 730-85$0^{\circ}C$. Carbon content of CVC powder controlled by the gas phase carburization at 85$0^{\circ}C$ was well matched with the theoretical carbon sioichiometry of WC, 6.13 wt％. During the gas phase carburization, the particle size of WC increased from 20 nm to 40 nm and the long chain structure of Co powders disappeared.

• Journal of Microbiology and Biotechnology
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• v.32 no.10
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• pp.1284-1291
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• 2022

The rise of methicillin-resistant Staphylococcus aureus (MRSA) has resulted in significant morbidity and mortality, and clinical treatment of MRSA infections has become extremely difficult. Sortase A (SrtA), a virulence determinant that anchors numerous virulence-related proteins to the cell wall, is a prime druggable target against S. aureus infection due to its crucial role in the pathogenicity of S. aureus. Here, we demonstrate that isovitexin, an active ingredient derived from a variety of traditional Chinese medicines, can reversibly inhibit SrtA activity in vitrowith a low dose (IC₅₀=24.72 ㎍/ml). Fluorescence quenching and molecular simulations proved the interaction between isovitexin and SrtA. Subsequent point mutation experiments further confirmed that the critical amino acid positions for SrtA binding to isovitexin were Ala-92, Ile-182, and Trp-197. In addition, isovitexin treatment dramatically reduced S. aureus invasion of A549 cells. This study shows that treatment with isovitexin could alleviate pathological injury and prolong the life span of mice in an S. aureus pneumonia model. According to our research, isovitexin represents a promising lead molecule for the creation of anti-S. aureus medicines or adjuncts.