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http://dx.doi.org/10.12925/jkocs.2021.38.6.1466

Anti-inflammatory activity and toxicity of the compound K produced by bioconversion  

Kim, MooSung (Macrocare Co., Ltd)
Shin, Hyun Young (Department of Integrated Biomedical and Life Science, Graduate School, Korea University, Transdisciplinary major in Learning Health System)
Kim, Hyun-Gyeong (Division of Food Science and Biotechnology, KNUT)
Kang, Ji Sung (Division of Food Science and Biotechnology, KNUT)
Jung, Kyung-Hwan (Division of Food Science and Biotechnology, KNUT)
Yu, Kwang-Won (Division of Food Science and Biotechnology, KNUT)
Moon, Gi-Seong (Division of Food Science and Biotechnology, KNUT)
Lee, Hyang-Yeol (Division of Food Science and Biotechnology, KNUT)
Publication Information
Journal of the Korean Applied Science and Technology / v.38, no.6, 2021 , pp. 1466-1475 More about this Journal
Abstract
Compound K (20-O-β-(D-glucopyranosyl)-20(S)-protopanaxadiol) is an active ingredient of ginsenosides. Compound K has been known to produce from biotransformation by β-glucosidase action of human intestinal microbes after oral admistration of ginseng. We have investigated the cytotoxicity of compound K obtained from bio-converted ginseng extract. As a result, compound K showed no significant cytotoxicity in the concentration of 0.001 to 1 ㎍/mL and inhibited the production of TNF-α, MCP-1, IL-6 and NO in RAW 264.7 cells induced by LPS inflamation. In the same concentration, HaCaT cells induced by inflammation with TNF-α and IFN-γ decreased IL-8 production due to compound K treatment. In the brine shrimp lethality assay, the LC50 of compound K was 0.37 mg/mL indicating some toxicity, but the bioconverted product containing 35% compound K showed relatively low toxicity with an LC50 of 0.87 mg/mL. These results suggest that the compound K enriched extract is a potential functional material for acne relief cosmetic products.
Keywords
Ginsenoside; Compound K; Bioconversion; Panax ginseng; Brine shrimp lethality assay;
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