• Journal of Plant Biology
• /
• 제30권3호
• /
• pp.189-199
• /
• 1987

Morpho-histogenesis of fruit sculpture and dehiscence in Thespesia populnea is described. The fruit wall is differentiated into epicarp, mesocarp and endocarp. The epicarp is stony, rind-like, 30 to 35 layers thick and derived from outer epidermis, sub-epidermis and ground parenchyma of the ovary wall. The spherical and/of tangentially elongated, thick walled cells of epicarp are interspersed with radial bands of sclereids. The mesocarp is a product of the inner zone of ground parenchyma. At maturity 20 to 25 layers of thin walled parenchyma of mesocarp appear sinuous of disorgnized. The innermost 1 to 3 layers of ground parenchyma and sub-epidermis and inner epidermis form 35 to 40 layers thick endocarp. Due to the differentiation of fibrous tissue in the projection of median plane of carpel wall and a complete ring of fibrous zone in the endocarp, the dry capsule of Thespesia populnea dehises partially in loculicidal fashion.

• Applied Microscopy
• /
• 제20권1호
• /
• pp.1-16
• /
• 1990

The epidermal tissue of Korean planaria Phagocata vivida(Ijima et Kaburaki) is composed of the simple columnar epithelium. The ventral epidermis of this animals is thinner than the dorsal epidermis and has a furrow in the median line in which dark cell is observed. The clear cells which are electron-lucent are located either side of the dark cells. Those are compactly covered with long cilia. The free surface of the latero-ventral epidermis is tightly contacted with the earth and this epidermal free surface has a great number of short cilia, and a lot of C-type of basophilic granule cell are migrated into the cytoplasm of epithelium from mesenchyme passing through the basement membrane and then this granules are put out of latero-ventral free surface. Dorsal epidermis is thickest among the whole epidermis of these animals and the rhabdite granules are more distributed in dorsal epidermis than in ventral epidermis. According to the cytochemical and ultrastructural research, composed epidermis of this planaria are divided into nine type cells, that is, ciliated columnar epithelium, dark cell, mucous cell, rhabdite-forming cell, sunk round cell, A type, B type, C type and D type of basophilic granule cell.

• Journal of Ginseng Research
• /
• 제24권3호
• /
• pp.107-112
• /
• 2000

적변삼 표피조직의 적갈색의 침적물은 강력한 산화제에 의해서만 산화(탈색)되는 chelation power가 매우 큰 화학결합을 형성하고 있었다. CLSM으로 관찰한 결과, 적변삼 조직의바깥쪽으로 두껍게 침적물이 형성되어 있음이 확인되었으며, 조직의 형광강도를 이용하여 두께를 측정한 결과 약 120rm의 침적물이 축적되어 있음이 확인되었다. 또한 Spectrum분석 결과 적변삼 표피의 물질은 건강삼의 표피조직과는 다른 functional group을 포함하는 것으로 확인되었다. 건강삼과 적변삼의 표피조직을 강산과 강염으로 가수분해 하여도 조직에 침적되어 있는 적색의 물질은 용출되지 않았다. 적변삼과 건강삼의 표피조직과 뿌리의 각 부위별 총 페놀성화합물의 함량을 측정한 결과, 건강삼의 표피조직에서 가장 높게 나타났다. 이러한 결과는 페놀성 물질이 적변물질의 원인이 된다는 보고와 차이가 있는 것으로 페놀성 물질은 적변현상 유발의 직접적인 원인물질이 아님이 확인되었다. 따라서 인삼의 적변물질은 인삼뿌리의 유기성분(세포벽 분해산물, 세포내용물, azodicarbonamide 등)과 철이온 복합체로 추론되며, 이물질에 대한 계속적인 추적을 진행하고 있다.

• Journal of Plant Biology
• /
• 제34권1호
• /
• pp.1-8
• /
• 1991

A sequential sub-cellular study of the epicarp of Nerium indicum has been performed. Outer epidermis of the ovary wall is covered externally with a thin cuticle. Cytoplasm possesses most of the cell organelles in the ovary stage itself. Outermost zone of the pericarp is the epicarp, developing from the outer epidermis. In the developing fruit, cell organelles are found with its maximum intensity. In mature fruit, the epicarp becomes multilayered due to additional development of few collenchymatous cells close to the outermost layer. Epicarpic cell possesses large central vacuole, around which a thin layer of cytoplasm is present. Number of cell organelles are considerably reduced in the mature fruit. In the ovary stage starch grains are electron transparent, while in the mature fruit it is fruit it is electron transluscent.

• 농업과학연구
• /
• 제9권2호
• /
• pp.453-460
• /
• 1982

개나리에 있어서 $AgNO_3$ 용액살포(溶液撒布)가 $SO_2$가스의 피해경감(被害輕減)에 미친는 효과(效果)를 시험(試驗)하였던 바 다음과 같은 결과(結果)를 얻었다. 1. $AgNO_3$ 용액(溶液)은 살포농도(撒布濃度)가 200 ppm 이상으로 되면 잎의 뒷면에 미세(微細)한 흑색반점(黑色斑點)을 발생(發生)시켰으며 100ppm으로 처리(處理)되었을 때 $SO_2$가스 피해(被害)로 일어나는 낙엽현상(落葉現狀)과 가시피해(可視被害)를 현저(顯著)히 경감(輕減)시켰다. 2. $AgNO_3$ 용액살포(溶液撒布)는 $SO_2$가스 피해(被害)로 발생(發生)되는 pH의 저하(低下)와 chlorophyll 함량(含量)의 감소현상(減少現狀)을 억제(抑制)시켰으며, peroxidase의 활성(活性)을 증가(增加)시켰다. 3. $AgNO_3$ 용액(溶液)의 살포(撒布)는 $SO_2$가스의 흡수(吸收)와 기공(氣孔)의 개폐(開閉)에 영향(影響)을 미치지 않았다.

• 한국자원식물학회지
• /
• 제30권1호
• /
• pp.88-95
• /
• 2017

토양수분의 변화가 블랙쵸크베리 'Nero'의 과실 특성에 미치는 영향을 조사하여 고품질 블랙쵸크베리 재배 연구의 기초자료를 제공하고자 수행하였다. 토양수분 감소로 인한 건조스트레스는 수확기 동안 블랙쵸크베리의 과실의 과중, 당도, 안토시아닌 함량 등의 품질을 저하시켰다. 또한 건조기간이 길수록 이후 토양수분 공급시, 과실 표피 세포 크기는 감소하는 반면 아표피, 과육 세포의 크기가 증가하여 열과를 유발할 수 있다. 따라서 관수시설을 통한 일정 주기의 관수가 블랙쵸크베리 과실 생산량 및 품질 향상을 위해 필요할 것으로 생각된다.

• 원예과학기술지
• /
• 제30권1호
• /
• pp.1-5
• /
• 2012

본 연구는 복숭아 '진미'의 미세균열 발생원인을 구명하기 위하여 시기별로 외과피 조직의 발달양상을 관찰하였으며, 수확기에는 '가납암백도', '천중도백도', '유명'과 외과피 조직의 차이를 비교하였다. 미세균열 발생은 '진미'에서 59.1%로 가장 높았고, '가납암백도' 30.6%, '천중도백도' 21.5%, '유명' 6.4%의 순이었다. '진미'는 만개 후 69일부터 외표피 바로 아래에서 공동화 현상이 쉽게 관찰되었으며, 생육이 진전될수록 공동화 현상이 확대되었다. 또한 수확기에 '진미' 품종은 외과피 층의 수가 다른 품종들에 비하여 적었으며, '천중도백도'와 '유명'에 비하여 외과피층의 두께가 얇고, 아표피 세포들의 크기가 작으며, 모양이 납작하였다. 따라서 복숭아 '진미'의 미세균열은 다른 품종에 비해서 외표피층의 발달 불량과 외과피 바로 아래 부분의 공동화 현상 발달에 기인한 것으로 판단되었다.

• 대한화장품학회지
• /
• 제46권3호
• /
• pp.307-317
• /
• 2020

피부 표피의 장벽 구조는 신체 내부의 수분 손실을 방지하고 외부의 이물질의 침입을 막는데 핵심적인 구조이고 각질세포와 세포간 지질로 구성된다. 이 때, 피부 장벽의 세포간 지질은 orthorhombic 구조로 패킹되어있을 때 가장 강한 구조를 가지고 있다. 그러나 다양한 외부 원인에 의해 손상되어 hexagonal 구조로 변화한다. 이런 물리적인 구조의 변화는 FT-IR을 이용하여 지질의 CH₂-CH₂ scissoring band의 시그널은 분석하여 비 침습적으로 분석 가능하다. 본 연구에서는 SDS를 Porcine skin에 처리하여 피부 장벽 손상모델을 구축하고 FT-IR signal을 분석하여 패킹구조의 변화 정도를 수치화 하였다. 이후 화장품 제형의 처리에 따라서 손상 모델의 장벽 회복 여부를 판단하였다. 이를 통하여 기존의 피부 장벽의 수분 증발량을 분석하는 간접적인 측정 방식을 보완할 수 있다. 또한, 피부 장벽 구조의 물리적인 변화를 직접적이고 효율적으로 분석하는 방식으로 활용 가능하여 다양한 소재의 기능 규명과 제형의 검증에 사용될 수 있다.

• Applied Microscopy
• /
• 제18권2호
• /
• pp.1-20
• /
• 1988

The species of the slug used in the experiment is Limax flavus L. For identifying the chemical characteristics of the epidermis, granules and mucus-producing cell of this animal is examined with methylene blue-basic fuchsin double stain and PAS-alcian blue reagent. For the ultrastructural research of the epidermal free surface, the epitheial cell and the parenchymal cell are used with scanning electron microscope and transmission elec-tron microscope respectively. I . Epidermal tissue The epidermal tissue of the slug is observed being divided into the dorsal and the ventral side(toot pad) respectively. 1) Dorsal epidermal tissue The dorsal epidermis of the slug is constituted with the simple columnar epithelium and the microvilli are compacted on the epidermal free surface. Two different types of the secretory granules of the neutral and the acid mucus are observed between the epithelial cells, and the neutral mucous granules are highest electron-dense but the acid mucous granules are observed to be electron-lucent. 2) Foot epidermal tissue The Foot epidermis is formed with the taller simple columnar epithelium than the dorsal epidermis and these cells have both a large number of the microvilli and a few number of the large villi. The secretory granules of three different types, which are acid, neutral and mixed mucous granule of two different types are observed between the epithelial cells. The neutral mucous granules are highest electron dense but the acid mucous granules are observed to be electron-lucent. II . Mucous granule-producing cell and mucus-producing cells Seven different types of the granules-producing cell and the mucus-producing cells are observed between the parenchyma. 1) A-type of acid mucous granule-producing cell The electron-lucent granules are largely occupied in the cytoplasm of these cells and then the granules are surrounded by irregular membrane. These electron-lucent granules exhibit alcianophilia with PAS-alcian blue reaction, so these granules are certified to be acid mucopolysaccharide. 2) B-type of acid mucus-producing cell The nucleus and the cytoplasm of these cells are pushed by the acid mucus of the electron-lucent toward the cell membrane. This mucus has been confirmed to be the acid mucopolysaccharide with PAS-alcian blue reagent. 3) A-type of neutral mucous granule-producing cell These cells contain the electron-dense round granules with approximately $1{\mu}m$ in diameter, which exhibit strongly PAS-positive reaction. These granules are confirmed to be the neutral mucoplysaccharide. 4) B-type of neutral mucous granule-producing cell These cells contain two different types of electron dense granules and electron-lucent granules; The former exhibits to be strongly PAS-positive and the latter to have alcianophilia reaction respectively. 5) C-type of neutral mucus-producing cell These cells are similar to the shape and the size of the B-type of mucus-producing cell but these two different types of cells are stained with reversing properties to each other. The mucus of the C-type cell that electron-lucent is largely occupied in the cytoplasm that exhibits strongly PAS-positive reaction. 6) D-type of neutral mucous granule-producing cell These cells contain round granules about $1{\mu}m$ in size which are observed to be medium electron-dense granules and those granules are stained brightly red with PAS-weak positive reaction. The granules are certified to be neutral mucopolysaccharide. 7) E-type of neutral mucous granule-producing cell These cells are similar to the shape and the size of the D-type of neutral mucous granule-producing cell. These cells contain a large number of granules with about $1{\mu}m$ in diameter showing electron-lucent and then granules are seen to be PAS-weak positive reaction. III. Parenchyma The clear cell and dark cell are found in the parenchyma of the Limax flavus L. 1) Clear cell These cells are round formed and the nucleus of the cells are larger than cytoplasm. These cells which have the electron-lucent cytosol possess poorly developed organelles. 2) Dark cell These cells are found to be dark cells due to high electron-density, which exhibit strongly methylene-blue reaction from double stain of methylene blue-basic fuchsin.

• Applied Microscopy
• /
• 제27권3호
• /
• pp.321-332
• /
• 1997

The dermal papilla is known to playa major role in influencing the form and dynamics of the hair follicle, which probably involves regulatory substances crossing the basal lamina. But little is known about the junctions between the dermal papilla and the surrounding epithelial cells of the hair bulb, or between the connective tissue and the epithelial cells on the outside of the hair follicle. This study was performed to identify the ultrastructural differences between dermoepidermal junction of the skin and connective tissue-epithelial junctions on the outside of the hair follicle and around the dermal papilla of normal anagen hair follicles in the human fetal scalp skin. Electron microscopic findings of dermoepidermal junction in scalp skin showed that basal lamina was very irregular and undulated, and it contained many attachment plaques of hemidesmosomes with sub-basal dense plates, tonofilaments, and anchoring filaments. Also invaginations of plasma membrane of basal keratinocytes were seen. There were clear differences both on the outside of the follicle and around the dermal papilla as compared with similar junction in the skin. In particular, neither hemidesmosomes nor tonofilaments, as seen in dermoepidermal junction, were observed in the dermal papilla. Also attachment plaque, sub-basal dense plate and anchoring filaments were not observed at the junction on the outside of the follicle and the dermal papilla. There were some differences between connective tissue-epithelial junctions on the outside of the hair follicle and around the dermal papilla, ie, smoothness of basal lamina and orthogonal arrangement of collagen fibers were seen in the outside of hair follicle, but not in the dermal papilla. These results indicate that the mechanical connection between the hair follicle and the connective tissue component is much weaker than that between the corresponding components in skin, and it reflects the dynamic processes during the anagen phase of the hair follicle compared to the relatively permanent state of the epidermis.