• Journal of Plant Biology
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• 제38권4호
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• pp.359-364
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• 1995

As the first step to elucidate the relationship between the structure and function of CTP:phosphocholine cytidylyltransferase (EC 2.7.7.15) in plants, the partial nucleotide sequence of soybean cytidylyltransferase cDNA was determined using a polymerase chain reaction (PCR). Degenerate oligonucleotide primers were synthesized from the conserved region revealed from the rat and yeast cytidylyltransferase DNA sequences. The catalytic domain region showed 78 and 76% homology with the rat and yeast amino acid sequences, respectivly. The hydropathy profile indicated that the C-terminal non-catalytic portion of the protein was very hydrophilic, and in the region between the catalytic domain and the C-terminal region, there was a large amphipathic $\alpha$-helical domain that was believed to bind the membrane surface in the active formation. There are 7 potential sites for phosphorylation by protein kinase C and 4 potential sites for phosphorylation by Ca2+/calmodulin kinase within the determined sequence.

• 경영과학
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• 제10권1호
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• pp.193-205
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• 1993

This paper presents a mathematical model for a class of vertical integration decisions. The problem structure of interest consists of raw material vendors, components suppliers, components processing plants, final product (assembly) plants and external components buyers. Economic feasibility of operating components plants instead of keeping outside suppliers is our major concern. The model also determines assignment of product lines and production volumes to each open plant considering the cost impacts of economies of scale and plant complexity. The problem formulation leads to a concave, mixed integer mathematical program. Given the state of the art of nonlinear programming techniques, it is often not possible to find global optima for reasonably sized such problems. We developed an optimization solution algorithm within the framework of Benders decomposition for the case of a piecewise linear concave cost function. It is shown that our algorithm generates optimal solutions efficiently.

• Journal of Plant Biology
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• 제33권1호
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• pp.81-84
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• 1990

The chloroplast has been the prime light-energy harvesting organelle on earth. It also carries out several key metabolic processes, such as lipid synthesis and nitrogen metabolism. Even though the chloroplast has its own genome, its coding capacity can afford only dozens of proteins, and most of the proteins functioning in the chloroplast are imported from the cytosol where nuclear encoded chloroplast genes are synthesized on free cytosokic ribosomes. Precursor proteins synthesized on cytosolic ribosomes have transit peptides at the amino termini of the proteins, and the transit peptide is sufficient to transfer chloroplast proteins from the cytosol into the chloroplast. When comparing amino acid sequences duduced from the nucleotide sequences of the clones of the chloroplast proteins, high homologies can be found among the transit peptides of proteins with the same function. Overall amino acid compositions of the transit peptides show amphiphilic characters of the transit peptides, and the amphiphilicity indicates that three dimensional structure of the transit peptide is responsible for the translocation of the chloroplast proteins.

• 한국전산구조공학회논문집
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• 제24권6호
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• pp.715-722
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• 2011

본 논문에서는 항공기 충돌에 의한 원전 격납건물의 거동을 병렬해석을 통해 수행하였다. 지금까지의 원전 격납건물에 대한 항공기 충돌관련 연구는 항공기의 경우, Riera의 충격하중-시간함수를 이상화하여 대상 구조체의 일정영역에 대해 충격하중으로 적용하는 방법을 사용해 왔고 충돌대상 구조체의 경우, 단순 철근콘크리트 벽체나 빌딩에 머물러 왔다. 하지만 본 논문에서는 항공기(Boeing-767, http://www.boeing.com)와 가상의 원전 격납건물을 실제와 유사하게 모델링하여 해석을 수행하였으며, 항공기모델은 충돌평가 가이드인 NEI 07-13(2009)에서 허용하는 Riera의 식에 따른 충돌하중이력곡선과 비교하는 방법으로 검증되었다. 또한, 일반적으로 고속 충돌해석은 짧은 시간동안 두 개 이상의 물체가 접촉하고 동적 대변형을 일으키는 비선형성이 강한 문제로 많은 계산시간이 요구되기 때문에 이를 효과적으로 다루기 위해서는 단일 CPU만으로는 한계가 있다. 따라서 본 논문에서는 해석의 효율성을 향상시키기 위해 자체 구축한 리눅스 클러스터 시스템을 이용하여 Message-Passing MIMD 형태의 병렬해석을 수행하였고 병렬성능에 대한 평가를 위해 무근콘크리트(Plain Concrete, PC), 철근콘크리트(Reinforced Concrete, RC), 내부 Liner Plate를 부착한 철근콘크리트(RC with Containment Liner Plate, CLP), SC구조(Steel-Plate Concrete, SC)등 4가지 경우에 대한 수치해석 효율성이 비교 검토되었다.

• 대한조선학회논문집
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• 제50권2호
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• pp.104-110
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• 2013

In this paper, the design procedure and results for 'Residual Life Prediction System Considering Corrosion and Coating' are explained, which is one module of 'Life-cycle Management System of Ship and Offshore Plant's' Operation. This 'Residual Life Prediction System' has two main functions; one is residual life prediction function based on probability processing using corrosion measurement data of ship's major structural members, and another is rust rate prediction function based on visual image processing of inspection photos. The analysis of system user requirements and functions are introduced, and the structure and environment of the developed system are explained.

• 한국식물학회:학술대회논문집
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• 한국식물학회 1987년도 식물생명공학 심포지움 논문집 Proceedings of Symposia on Plant Biotechnology
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• pp.149-155
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• 1987

Growth and development of a higher plant, or any living organism for that matter, could be defined as an orderly expression of the genome in time and space in close interaction with the environment. During differentiation and development of a tissue or organ a group of genes must be selectively turned on or turned off mainly by trans-acting regulators. In this general concept of regulation of regulation of gene expression, a DNA molecule is recognized at a specific nucleotide sequence by DNA-binding factors. Molecular biology of the regulatory factors such as hormones, and their receptors, target DNA sequences and DNA-binding proteins are well advanced. What is not clearly understood is the molecular basis of the interactions between DNA and binding factors, expecially of the usages of the dyad symmetry of the target DNA sequences and the dimeric nature of the DNA-binding proteins. A unique 3-dimensional structure of DNA has been proposed that may play an important role in the orderly expression of the gene. A foldback intercoil (FBI) DNA configuration which was originally found by electron microscopy among mtDNA molecules from pearl millet has some unique features. The FBI configuration of DNA is believed to be formed when a flexible double helix folds back and interwines in the widened major grooves resulting in a four stranded, intercoil DNA whose thickness is the same as that of double stranded DNA. More recently, the FBI structure of DNA has been also induced in vitro by a novel enzyme which was purified from pearl millet mitochondria. It has been proposed that the FBI DNA could be utillized in intramolecular recombination which leads to inversion or deletion, and in intermolecular recombination which can lead to either site-specific recombination, genetic recombination via single strand invasion, or cross strand recombination. The structure and function of DNA in 3-dimensional aspect is emphasized for better understanding orderly expression of genes during growth and development.

• 한국지능시스템학회:학술대회논문집
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• 한국퍼지및지능시스템학회 1998년도 추계학술대회 학술발표 논문집
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• pp.532-538
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• 1998

In this paper, a new adaptive fuzzy inference method using neural network based fuzzy reasoning is proposed to make a fuzzy logic control system more adaptive and more effective. In most cases, the design of a fuzzy inference system rely on the method in which an expert or a skilled human operator would operate in that special domain. However, if he has not expert knowledge for any nonlinear environment, it is difficult to control in order to optimize. Thus, using the proposed adaptive structure for the fuzzy reasoning system can controled more adaptive and more effective in nonlinear environment for changing input membership functions and output membership functions. The proposed fuzzy inference algorithm is called adaptive neuro-fuzzy control(ANFC). ANFC can adapt a proper membership function for nonlinear plant, based upon a minimum number of rules and an initial approximate membership function. Nonlinear function approximation and rotary inverted pendulum control system ar employed to demonstrate the viability of the proposed ANFC.

• Journal of Ecology and Environment
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• 제29권3호
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• pp.247-252
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• 2006

울릉도의 특산 식물종으로서 희귀식물종이며, 춘계 단명성 식물로서 개미에 의해 종자 산포가 이루어지는 섬현호색(Corydalis filistipes Nakai) 4개의 아집단에 대하여 합리적인 보전 및 관리 대책 수립을 위하여 9 개의 동위효소 marker를 이용하여 유전적 다양성과 구조를 분석하였다. 그 결과 평균 대립 유전자의 수(A)는 1.73개, 95% 수준에서 다형적 유전자좌의 비율은(P)은 61.2%, 이형접합체의 평균 관측치(Ho)는 0.201, 기대치(He)는 0.167로서 분포역이 넓은 특산 식물 종들에 비해서는 낮은 수준이지만, 섬에 고립된 유사한 생활사를 갖는 특산식물 종들에 비해서는 높은 유전적 다양도를 유지하고 있는데, 그 이유는 소집단이기는 하나 적정수준의 개체수가 유지되고 있고, 타가수정을 주로 하며 개미에 의해 종자 산포가 이루어져 적응력이 높고, 또한 폐쇄화에 의한 유 무성 번식 체계를 겸하기 때문인 것으로 판단된다. 그리고 유전적 구조분석 결과 아집단내($F_{IS}$)와 전체 아집단($F_{IT}$)의 근친교 배계수가 각각 -0.1889 와 -0.1226로서 H-W의 평형구조에 비해 이형접합자의 빈도가 높았으며, 아집단간 유전적 분화도는 매우 낮은($F_{ST}=0.0557$)결과를 보였다. 그리고 우리나라 희귀 및 특산 식물종인 섬현호색의 유전적 변이의 유지 기작과 합리적인 보전과 관리 대책을 논의하였다.

• 전기의세계
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• 제22권4호
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• pp.17-24
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• 1973

In the view point of practical engineering the identification problem may be considered as a problem to determine the optimal model in the sense of minimizing a given criterion function using the input-output records of the plant. In the system identification the statistical approach has been known to be very effective when the topological structure of the system and the statistical characteristics of the observation noises are known a priori. But in the practical situation there are many cases when the inforhation about the observation noises or the system noises are not available a priori. Here, the authors propose a new identification method which can be used effectively even in the cases when the variances of observation noises are unknown a priori. In the method, the identification of unknown parameters of a linear diserete system is achieved by minimizing the improved quadratic criterion function which is composed of the term of square equation errors and the term to eliminate the affection of observation noises. The method also gives the estimate of noise variance. Numerical computations for several examples show that the proposed procedure gives satisfactory results even when the short time observation data are provided.

• 대한수의학회지
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• 제58권4호
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• pp.211-217
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• 2018

Mesenchymal stem cells (MSCs) are useful candidates for tissue engineering and cell therapy. Physiological cell environment not only connects cells to each other, but also connects cells to the extracellular matrix that provide mechanical support, thus exposing the entire cell surface and activating signaling pathways. Hydrogel is a polymeric material that swells in water and maintains a distinct 3-dimensional (3D) network structure by cross linking. In this study, we investigated the optimized cellular function for canine adipose tissue-derived MSCs (cAD-MSCs) using hydrogel. We observed that the expression levels of Ki67 and proliferating cell nuclear antigen, which are involved in cell proliferation and stemness, were increased in transwell-hydrogel (3D-TN) compared to the transwell-normal (TN). Also, transforming growth factor-${\beta}1$ and SOX9, which are typical bone morphogenesis-inducing factors, were increased in 3D-TN compared to the TN. Collagen type II alpha 1, which is a chondrocyte-specific marker, was increased in 3D-TN compared to the TN. Osteocalcin, which is a osteocyte-specific marker, was increased in 3D-TN compared to the TN. Collectively, preconditioning cAD-MSCs via 3D culture systems can enhance inherent secretory properties that may improve the potency and efficacy of MSCs-based therapies for bone regeneration process.