• BMB Reports
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• 제42권10호
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• pp.623-630
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• 2009

Hsp90, an evolutionarily conserved molecular chaperone, is involved in the folding, stabilization, activation, and assembly of a wide range of 'client' proteins, thus playing a central role in many biological processes. Especially, several oncoproteins act as Hsp90 client proteins and tumor cells require higher Hsp90 activity than normal cells to maintain their malignancy. For this reason, Hsp90 has emerged as a promising target for anti-cancer drug development. It is still largely unknown how Hsp90 can recognize structurally unrelated client proteins. However, recent progress in structural studies on Hsp90 and its interaction with various co-chaperones has broadened our knowledge of how the Hsp90 ATPase activity, which is essential for its chaperone function, is regulated and coupled with the conformational changes of Hsp90 dimer. This review focuses on the roles of various Hsp90 co-chaperones in the regulation of the Hsp90 ATPase cycle, as well as in the selection of client proteins. In addition, the current development of Hsp90 inhibitors based on the structural information will be discussed.

• 한국전산구조공학회:학술대회논문집
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• 한국전산구조공학회 2001년도 봄 학술발표회 논문집
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• pp.53-60
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• 2001

Meshfree methods have been attracting issue as computational methods during past a few years. Nowadays, various meshfree methods such as EFGM, RKPM h-p cloud method and etc. were developed and applied in engineering problems. But, most of them were not truly meshless method because background mesh of cell was required for the spatial integration of a weak form. A nodal integration is required for truly meshless methods but it is known that this method gives a little unstable and incorrect solutions. In this paper, an improvement scheme of the existed nodal integration which the weak form can be simply integrated without any stabilization term is proposed. Numerical tests show that the proposed method is more convenient and gives more correct solutions than the previous method.

• Structural Monitoring and Maintenance
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• 제6권4호
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• pp.347-364
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• 2019

Two seismic response data from the CSMIP strong motion instrumentation of Pacoima dam are selected: San Fernando earthquake (Jan 13, 2001; ML=4.3) and Newhall earthquake (Sept. 1, 2011; ML=4.2), for the identification of the dam system. To consider the spatially nonuniform input ground motion along the dam abutment, the subspace identification technique with multiple-input and multiple-output is used to extract the dynamic behavior of the dam-reservoir interaction system. It is observed that the dam-reservoir interaction is significant from the identification of San Fernando earthquake data. The influence of added mass (from the reservoir) during strong ground motion will create a tuned-mass damper phenomenon on the dam body. The fundamental frequency of the dam will be tuned to two different frequencies but with the same mode shapes. As for the small earthquake event, the dam-reservoir interaction is insignificant.

• 한국전산구조공학회:학술대회논문집
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• 한국전산구조공학회 1997년도 봄 학술발표회 논문집
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• pp.152-159
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• 1997

This unit - structure of truss stabilized by cable tension is composed of four truss member adding to a central post and eight cables, and is connected by hinge joints and is stabilized by cable tension. As this unit - structures itself is a statically closed and stabilize system individually, it can be employed to assemble a structure with a variety of configuration. In this paper, for determination the shape of the unit - structure of truss stabilized by the cable, characteristics such as the stabilized range of the various geometrical parameter about unit system and the relation of the best governing paramter is demonstrated, and the relation of the results is compared in the range for the stabilization of unit-structure.

• Structural Engineering and Mechanics
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• 제5권6호
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• pp.785-801
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• 1997

Reinforced Concrete beams with tension and compression softening material constitutive laws are studied. Energy-based and non-local regularisation techniques are presented and applied to a R.C. element. The element characteristics (sectional tangent stiffness matrix, element tangent stiffness matrix restoring forces) are directly derived from their symbolic expressions through numerical integration. In this way the same spatial grid allows us to obtain a non-local strain estimate and also to sample the contributions to the element stiffness matrix. Three examples show the spurious behaviors due to the strain localization and the stabilization effects given by the regularisation techniques, both in the case of tension and compression softening. The possibility to overestimate the ultimate load level when the non-local strain measure is applied to a non softening material is shown.

• Structural Engineering and Mechanics
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• 제72권1호
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• pp.19-30
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• 2019

The mechanical behavior of Fiber Reinforced Cementitious Composites (FRCC) under direct shear is studied through experiment and analytical simulation. The cementitious composite considered contains 55% replacement of cement with fly ash and 2% (volume ratio) of short discontinuous synthetic fibers (in the form of mass reinforcement, comprising PVA - Polyvinyl Alcohol fibers). This class of cementitious materials exhibits ductility under tension with the formation of multiple fine cracks and significant delay of crack stabilization (i.e., localization of cracking at a single location). One of the behavioral parameters that concern structural design is the shear strength of this new type of fiber reinforced composites. This aspect was studied in the present work with the use of Push-off tests. The shear strength is then compared to the materials' tensile and splitting strength values.

• 한국항공우주학회지
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• 제46권5호
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• pp.419-426
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• 2018

심우주 탐사 임무를 수행하는 경우 발사체의 최상단에 궤도전이를 위한 고체 또는 액체 엔진이 장착된다. 궤도 전이 과정에서 추력 비정렬 오차가 존재할 경우, 시간이 지남에 따라 탑재체의 자세각 오차가 급속히 증가하여 임무의 성패에 직접적인 영향을 미칠 수 있다. 이러한 추력 비정렬 오차를 줄이는 방법으로는 스핀안정화 방식 또는 3축 자세제어 방식이 있으며, 이 중 시스템 단순화 및 구조 경량화 측면에서 강점을 가지는 스핀안정화 방식이 많이 사용된다. 스핀 안정화를 적용하는 방법 중 하나는 발사체의 스핀테이블 시스템을 사용하는 것이다. 본 연구에서는 스핀테이블이 탑재체 분리 후 거동에 미치는 영향을 분석하였다. 분석에 필요한 스핀테이블의 기능을 모사할 수 있도록 기초 설계를 수행하였으며, 이를 적용하여 스핀테이블의 거동을 모사하는 시뮬레이션 환경을 구축하였다. 스핀테이블의 유무에 따른 탑재체의 거동 및 스핀테이블의 효과를 분석하였고 스핀테이블 시스템의 구성요소 중 분리스프링의 공차에 의한 탑재체의 분리 후 거동 해석을 수행하였다.

• 한국구조물진단유지관리공학회 논문집
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• 제19권3호
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• pp.130-138
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• 2015

본 연구에서는 고유동 고강도 콘크리트의 현장타설에의 적용성 검토를 위해 거푸집의 측압검토를 수행하였다. 이를 위해 타설높이 및 타설속도를 주요변수로 정하여 실험체를 제작한 후, 수행한 실험으로부터 측압 및 온도변화를 측정하였다. 고유동 고강도 콘크리트의 최대 측압은 굳지 않은 콘크리트의 측압으로 나타났으며, 타설 직후 측압이 감소하기 시작하여 12시간 이후 안정화를 보였다. 3~4시간 이후 안정화가 나타나는 보통 콘크리트와 측압이 감소하는 경향은 유사하다. 타설속도가 빠를수록 최대 측압이 크게 발생하였으며, 시간의 경과에 따른 감소량은 거의 유사하였다. 또한 수화열과 측압감소 사이에 직접적인 연관성은 나타나지 않았다.

• 대한화학회지
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• 제29권5호
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• pp.533-538
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• 1985

Spermine에 의하여 誘發되는 凝縮 DNA 構造의 安定化에 있어서 hydrophobic interaction의 重要性을 試驗하기 위하여 spermine-DNA 複合體의 異例的 吸光度-溫度 樣相에 미치는 urea의 效果를 調査하였다. 그 結果 異例的 吸光度-溫度 樣相의 downward peak(trough) 領域에 이르는 相變移에 對한 cooperativity, enthalpy, midpoint의 값들이 Tm 領域의 값들 보다 urea에 敏感한 影響을 받았으며, urea의 濃度 增加에 따라 downward peak가 점차 사라졌다. 이 data는 異例的 吸光度-溫度 樣相을 構成하는 downward peak가 spermine에 의하여 誘發되는 hydrophobic interaction에 의한 凝縮된 tertiary structure의 形成을 나타내고 있다고 불 수 있다.

• Journal of Microbiology and Biotechnology
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• 제28권9호
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• pp.1457-1466
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• 2018

In the present study, the stabilizing effect of four different biological osmolytes on Bacillus licheniformis ${\gamma}$-glutamyl transpeptidase (BlGGT) was investigated. BlGGT appeared to be stable under temperatures below $40^{\circ}C$, but the enzyme retained less than 10% of its activity at $60^{\circ}C$. The tested osmolytes exhibited different degrees of effectiveness against temperature inactivation of BlGGT, and sucrose was found to be the most effective among these. The use of circular dichroism spectroscopy for studying the secondary structure of BlGGT revealed that the temperature-induced conformational change of the protein molecule could be prevented by the osmolytes. Consistently, the molecular structure of the enzyme was essentially conserved by the osmolytes at elevated temperatures as monitored by fluorescence spectroscopy. Sucrose was further observed to counteract guanidine hydrochloride (GdnHCl)-and urea-induced denaturation of BlGGT. Taken together, we observed evidently that some well-known biological osmolytes, especially sucrose, make a dominant contribution to the structural stabilization of BlGTT.