• Title/Summary/Keyword: strong thin layer

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Antioxidant and α-Glucosidase Inhibitory Activities of the Extract from Sparganium stoloniferum Buch.-Ham. Root and Its Constituent Compounds

  • Xu, Ming Lu;Wang, Lan;Hu, Jian He;Wang, Myeong-Hyeon
    • Preventive Nutrition and Food Science
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    • v.14 no.4
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    • pp.354-357
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    • 2009
  • Three compounds, vanillic acid, p-hydroxylcinnamic acid, p-hydroxybenzaldehyde have been isolated from the ethylacetate extract of Sparganium stoloniferum Buch.-Ham roots using silica gel open column chromatography, preparative thin-layer chromatography (pTLC) and reverse phase high performance liquid chromatography. The structures of the compounds were established on the basis of IR, extensive 1D NMR, and MS analyses. The ethylacetate (EtOAc) extract, vanillic acid, and p-hydroxybenzaldehyde showed $\alpha$-glucosidase inhibition activity of 72.71%, 20.13%, and 30.42%, at the concentration of 10 ${\mu}g/mL$, respectively. The EtOAc extract exhibited strong antioxidant activity with an $IC_50$ value of 24.37 ${\mu}g/mL$ against DPPH radical scavenging activity, the vanillic acid, p-hydroxylcinnamic acid, and p-hydroxybenzaldehyde with an $IC_50$ value of 2.10 ${\mu}M$, 1.59 ${\mu}M$, and 2.72 ${\mu}M$ against DPPH, respectively.

대두 사포닌의 활성에 관한 기전 연구

  • 성미경
    • Journal of Nutrition and Health
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    • v.28 no.10
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    • pp.1022-1030
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    • 1995
  • Saponins are glycosidic compounds present in many plant foods. They are characterized by their ability to lyse cell membranes due to their surface-active properties. Saponins are believed to interact primarily with cholesterol in the cell membrane. In this study, the interaction of soybean(SS) with cell membrane was investigated using erythrocytes as a model. Mechanisms of interaction was also investigated by measuring their binding capacity with different membrane lipid fractions. Throughout the study, gypsophilla saponin(GS) and quillaja saponin(QS) were used to evaluate the membranolytic activity of soybean saponins. All saponins released hemoglobin in a concentration-dependent manner. SS induced 40% hemolysis at the concentration of 400 ppm, however there was no increase in hemoglobin release above 400ppm concentration. 5ppm of GS and 8 ppm of QS hemolyzed 100% of erythrocytes. Isolation of SS fractions by thin layer chromatography revealed that only one non-polar saponin possesses strong hemolytic activity. When saponins were incubated decreased the release of cholesterol. When the hemolytic activity of saponins was measured in the presence of other major membrane lipid components, sphingomyelin significantly reduced the hemolytic activity of SS, while cholesterol reduced the activity of QS. GS showed high affinity to other component(s) in the incubation media as well as lipids. These results suggest that the membranolytic activity of saponins are related to their specific chemical structure, which determines the interaction behavior between saponins and different membrane components, and thereby influence the biological activity.

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Magnetohydrodynamic Simulations of Barred Galaxies

  • Kim, Woong-Tae;Stone, James M.
    • The Bulletin of The Korean Astronomical Society
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    • v.37 no.1
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    • pp.38.2-38.2
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    • 2012
  • We use two-dimensional high-resolution MHD simulations to investigate the effects of magnetic fields on the formation and evolution of such substructures as well as on the mass inflow rates to the galaxy center. We find that there exists an outermost x1-orbit relative to which gaseous responses to an imposed stellar bar potential are completely different between inside and outside. Inside this orbit, gas is shocked into dust lanes and infalls to form a nuclear ring. Magnetic fields are compressed in dust lanes, reducing their peak density. Magnetic stress removes further angular momentum of the gas at the shocks and leads to a smaller and more centrally distributed ring, resulting in the mass inflow rates larger, by more than two orders of magnitude, than in the unmagnetized counterparts. Outside the outermost x1-orbit, on the other hand, an MHD dynamo operates near the corotation and bar-end regions, efficiently amplifying magnetic fields. The amplified fields shape into trailing magnetic arms with strong fields and low density. The base of the magnetic arms have a thin layer in which magnetic fields with opposite polarity reconnect via a tearing-mode instability. This produces numerous magnetic islands with large density which propagate along the arms to turn the outer disk into a highly chaotic state.

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Transparent Conducting Multilayer Electrode (GTO/Ag/GTO) Prepared by Radio-Frequency Sputtering for Organic Photovoltaic's Cells

  • Pandey, Rina;Kim, Jung Hyuk;Hwang, Do Kyung;Choi, Won Kook
    • Journal of Sensor Science and Technology
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    • v.24 no.4
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    • pp.219-223
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    • 2015
  • Indium free consisting of three alternating layers GTO/Ag/GTO has been fabricated by radio-frequency (RF) sputtering for the applications as transparent conducting electrodes and the structural, electrical and optical properties of the gallium tin oxide (GTO) films were carefully studied. The gallium tin oxide thin films deposited at room temperature are found to have an amorphous structure. Hall Effect measurements show a strong influence on the conductivity type where it changed from n-type to p-type at $700^{\circ}C$. GTO/Ag/GTO multilayer structured electrode with a few nm of Ag layer embedded is fabricated and show the optical transmittance of 86.48% in the visible range (${\lambda}$ = 380~770 nm) and quite low electrical resistivity of ${\sim}10^{-5}{\Omega}cm$. The resultant power conversion efficiency of 2.60% of the multilayer based OPV (GAG) is lower than that of the reference commercial ITO. GTO/Ag/GTO multilayer is a promising transparent conducting electrode material due to its low resistivity, high transmittance, low temperature deposition and low cost components.

A Study on the Magnetic Properties and Microstructure of CoCr Thin Films Growing under Magnetic Field (자장하에서 성장한 CoCr박막의 자기적 특성 및 미세구조에 관한 연구)

  • Lee, Yu-Gi;Jang, Pyeong-U;Lee, Taek-Dong;Lee, Gye-Won
    • Korean Journal of Materials Research
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    • v.4 no.5
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    • pp.581-589
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    • 1994
  • Magnetic properties and microstructures of the $Co_{83}Cr_{17}$ films growing under the applied magnetic field were studied. In comparison, those of the films growing without magnetic field were also studied. Magnetic field does not affect saturation magnetization and in-plane coercivity of the films. On the contrary perpendicular coercivity and effective perpendicular anisotropy field decreased. Grain size and the thickness of the so-called transition layer were not affected and the C-axis alignment of the films was slightly deteriorated due to magnetic field. Also, microstructures of the sputtered films showed larger grain sizes of strong (002) preferred orientation for thicker film specimens independent of applied magnetic field.

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The Nitro-scavenging Effects by the Component of Cassiae Torae Semen (결명자의 아질산염 소거작용)

  • Do, Jeong-Ryong;Kim, Seon-Bong;Park, Yeung-Ho;Park, Yeung-Beom;Choi, Jae-Sue;Kim, Dong-Soo
    • Korean Journal of Food Science and Technology
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    • v.25 no.5
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    • pp.526-529
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    • 1993
  • The present study was conducted to investigate nitrite-scavenging ability of the components of Cassiae torae semen, Cassia tora L. The nitrite-scavenging effects of fractions obtained from methanol extract of Cassiae torae semen, Cassia tora L. were strong in ethyl acetate fraction, chloroform fraction, water soluble fraction and ethyl ether fraction in order. Compound D showed the strongest nitrite-scavenging effects among compound A, B, C, D separated from Cassiae torae semen. Compound D separated from Cassiae torae semen possessed ten-fold stronger the nitrite-scavenging effect than ascorbic acid.

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In vitro and In vivo Effects of Gelidium amansii on Intestinal Immune System

  • Jun, Woo-Jin;Kim, Se-Han;Lee, Dae-Hee;Chun, Jin-Woong;Sim, Sang-In;Lee, Kwang-Won;Cho, Hong-Yon;Hong, Bum-Shik
    • Food Science and Biotechnology
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    • v.14 no.1
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    • pp.147-151
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    • 2005
  • Purified compound with intestinal immune system-modulating properties, GWE-2c, was isolated from methanol extract of Gelidium amansii by sequential procedures with silica gel column, LH-20 Sephadex gel column, and thin-layer chromatographies. In the presence of GWE-2c, strong immunoactivity in Peyers patch cell-mediated bone marrow cells was observed in vitro. In vivo intestinal immune-modulating activity was also enhanced by crude phenolic compound (GWE) of G. amansii in a dose-dependent manner. Investigation of production of several cytokines in Peyer's patch cells upon stimulation with GWE in vivo revealed the levels of granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin (IL)-6 increased. Results suggest that the phenolic compound from G. amansii represents immunopotentiator and biological response modifier at in vitro and in vivo levels.

Numerical Analysis of Loss Power Properties in the Near-Field Electromagnetic Wave Through A Microstrip Line for Multilayer Magnetic Films with Different Levels of Electrical Conductivity

  • Lee, Jung-Hwan;Kim, Sang-Woo
    • Journal of Magnetics
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    • v.13 no.3
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    • pp.92-96
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    • 2008
  • There are few reports of high frequency loss behavior in the near-field for magnetic films with semiconducting properties, even though semiconducting magnetic materials, such as soft magnetic amorphous alloys and nanocrystalline thin films, have been demonstrated. The electromagnetic loss behavior of multilayer magnetic films with semiconducting properties on the microstrip line in quasi-microwave frequency band was analyzed numerically using a commercial finite-element based electromagnetic solver. The large increase in the absorption performance and broadband characteristics of the semiconducting/insulating layer magnetic films examined in this study were attributed to an increase in the loss factor of resistive loss. The electromagnetic reflection increased significantly with increasing conductivity, and the loss power deteriorated significantly. The numerical results of the magnetic field distribution showed that a strong radiated signal on the microstrip line was emitted with increasing conductivity and decreasing film thickness due to re-reflection of the radiated wave from the surface of the magnetic film, even though the emitted levels varied with film thickness.

Antagonism and Structural Identification of Antifungal Compound from Chaetomium cochliodes against Phytopathogenic Fungi

  • Kang, Jae Gon;Kim, Keun Ki;Kang, Kyu Young
    • Journal of Applied Biological Chemistry
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    • v.42 no.3
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    • pp.146-150
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    • 1999
  • As a part of the integrated disease system in greenhouse, an antifungal fungus(AF1) was isolated from greenhouse soil. It exhibited strong inhibitory activites against Pythium ultimum, Phytophtora capsici, Rhizoctonia solani, Botrytis cinerea, and Fusarium oxysporum based on dual culture on 1/5 strength of potato dextrose agar between antagonistic fungus and several plant pathogens. The antagonistic fungus was identified as Chaetomium cochliodes, based on morphological characteristics; the body of the perithecium bears straight or slightly wavy, unbranched hairs, whilst the apex bears a group of spirally coiled hairs. To investigate antagonistic principles, antifungal compound was extracted and fractionated by different solvent systems. An antifungal compound was isolated as pure crystal from is culture filtrate using organic solvent extraction and column chromatography, followed by preparative thin layer chromatography. The chemical structure of the purified antifungal compound was identified as chaetoglobosin A based on the data obtained form $^1H-NMR$, $^{13}C-NMR$, DEPT 90, 135, $^1H-^1H$ COSY, $^1H-^{13}C$ COSY and EI/MS. $ED_{50}$ values of the chaetoglobosin A against P. ultimum, P. capsici, R. solani, B. cinerea and F. oxysporum were 1.98, 4.01, 4.16, 2.67 and 35.14 ppm, respectively.

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Searching for Insecticidal Metabolites Produced by Insect Pathogenic Fungi (곤충기생성 진균이 생산하는 살충성 생리활성물질의 탐색)

  • Youn, Young-Nam;Yeo, Woon-Hyung;Seo, Mi-Ja
    • The Korean Journal of Mycology
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    • v.26 no.1 s.84
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    • pp.78-85
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    • 1998
  • During the screening of biological control agents for insect pests in the greenhouse, 70 dead insect-related fungi were isolated and tested insecticidal effect of their culture filtrates was tested. From this studies, CNAB-63 isolate showed strong control effects against the cotton aphids and the two-spotted spider mite as 65.19% and 77.55%, respectively. The insecticidal active compound of CNAB-63 isolate was purified from culture filtrate by silica gel chromotography, thin layer chromatography and HPLC. Purified active compound (CNAB) showed control effects against the two-spotted spider mite with 63.45% at $100\;{\mu}g/ml$ concentration. However, it did not exhibit antimicrobial activity against fungi and bacteria.

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