The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Searching for Insecticidal Metabolites Produced by Insect Pathogenic Fungi

곤충기생성 진균이 생산하는 살충성 생리활성물질의 탐색

  • Youn, Young-Nam (Division of Applied Biology and Chemistry, College of Agriculture, Chungnam National University) ;
  • Yeo, Woon-Hyung (Korea Ginseng & Tobacco Research Institute) ;
  • Seo, Mi-Ja (Division of Applied Biology and Chemistry, College of Agriculture, Chungnam National University)
  • 윤영남 (충남대학교 농과대학 응용생물화학부) ;
  • 여운형 (한국인삼연초연구소 원료3실) ;
  • 서미자 (충남대학교 농과대학 응용생물화학부)
  • Published : 1998.03.30
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Abstract

During the screening of biological control agents for insect pests in the greenhouse, 70 dead insect-related fungi were isolated and tested insecticidal effect of their culture filtrates was tested. From this studies, CNAB-63 isolate showed strong control effects against the cotton aphids and the two-spotted spider mite as 65.19% and 77.55%, respectively. The insecticidal active compound of CNAB-63 isolate was purified from culture filtrate by silica gel chromotography, thin layer chromatography and HPLC. Purified active compound (CNAB) showed control effects against the two-spotted spider mite with 63.45% at $100\;{\mu}g/ml$ concentration. However, it did not exhibit antimicrobial activity against fungi and bacteria.

전국 각지에서 채집한 사멸곤충에서 분리한 곤충 기생성 진균이 생산하는 살충성 생리활성물질을 탐색한 결과 곰팡이 분리주 CNAB-63 균주가 목화진딧물과 점박이응애 모두에 대해 다른 분리균주들보다 비교적 높은 살충효과를 보인 바, 본 균주가 생산하는 살충성분을 각종 chromatography로 분리 정제하였다. 정제된 활성물질 CNAB는 점박이응애에서 66.59%, 목화진딧물에서 52.96%의 살충효과를 보였으며, 그 외에도 온실가루이에 살충효과를 보였으나 배추좀나방에는 미미한 살충효과를 보였다. 한편 활성물질 CNAB는 $100\;{\mu}g/disk$ 농도에서 곰팡이, 효모, 세균류에 전혀 항균활성을 보이지 않았다.

Keywords

References

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