• 대한화장품학회:학술대회논문집
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• 대한화장품학회 2003년도 IFSCC Conference Proceeding Book I
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• pp.387-412
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• 2003

Influence of gelatinase on basement membrane (BM) structure was investigated by using a skin equivalent (SE) model. The results showed that (1) gelatinase produced by cells degraded the BM and (2) the addition of matrix metalloproteinase-specific inhibitor to the SE medium accelerated the formation of BM structure, indicating that gelatinase is involved in BM impairment. The activity of gelatinase was also studied in healthy human facial skin tissues. The result of in situ zymography revealed gelatinase activity around the basal layer of the epidermis, where BM integrity was severely compromised. Therefore, this enzyme was suggested to be associated with BM decomposition in human facial skin. To assess the behavior of gelatinase in stratum corneum (SC) non-invasively, an immunological study was performed. Since positive immunostaining of pro-gelatinase B was observed in SC stripped from sun-exposed skin, whereas no positive staining detected in SC of non-irradiated skin, gelatinase in the epidermis could be non-invasively detected by measuring gelatinase in SC. Gelatinase in SC of healthy female volunteers was monitored using a special film that sensitively and conveniently detects gelatinase. Ninetr percent of SC from facial skin (l00 women, 40's-50's) was gelatinase-positive. On the other hand, SC from non-irradiated skin was negative. These results strongly suggest that (1) gelatinase is constantly produced in the facial epidermis of most middle-aged woman during their daily life, and (2) the enzyme might be involved in the aging-related degeneration of both BM and the matrix fibers of the upper layer of the dermis, acting as a very important aging factor. Strong inhibitory activity against gelatinase was found in turmeric extract and identified curcumin as the major ingredient. Topical application of cream containing turmeric extract significantly decreased the number of gelatinase-positive SC clusters in human facial skins. These results indicated that turmeric is an effective ingredient to prevent skin from photo aging by suppressing chlonically upregulated gelatinase activity by UV and to improve skin condition.

• Journal of Biosystems Engineering
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• 제27권4호
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• pp.311-316
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• 2002

This study was carried out to investigate the optimum operating conditions of the air injection type onion peeling device which could be attached to a prototype onion peeler. An onion, stem and root was cut and some vertical line was dug in 1 mm depth on the skin, was put on the two parallel rollers. The diameters of the rollers were 105 mm and the ratio of peripheral velocity was 3:2, and moved by a geared motor. Air from the nozzle with high pressure and velocity was jetted to the rotating onion on the revolving rollers, and then the skin of the onion was stripped. On the test, the rolling characteristics of the experimental materials were measured. The effective peeling conditions were, the number of digging line on the skin of the onion was 4, and the air jet pressure was above 392.3 kPa(4.0 kg/$\textrm{cm}^2$) when the peripheral velocity was at 2.4 m/s. On these conditions, time requirement to peel an onion was less than 2 sec.

• Archives of Plastic Surgery
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• 제34권5호
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• pp.587-592
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• 2007

Purpose: In case of postburn flexion contracture of the fingers, skin graft, geometrical relaxation techniques, local flap, and free flap have been used. Among these procedures, full-thickness skin grafts from the inguinal area are widely used to reconstruct a postburn flexion contracture in the fingers. But there are many esthetic and functional problems in this procedure. Especially, hyperpigmentation of the skin-grafted fingers poses a troublesome problem, particularly in the patients who have dark colored skin. To solve the problem, we have used pulp graft which was harvested from the lateral aspect of great toe. In the present study, we report pulp graft, with which we have obtained a good result in the treatment of postburn flexion contracture of the fingers. Methods: Between September of 2004 and August of 2006, great toe pulp graft was performed to 20 sites of 15 patients. After release of the postburn flexion contracture using Z-plasty, the composite tissue (pulp) harvested from the lateral aspect of great toe was grafted on the raw surface. Moisture dressing with ointment and foam dressing material was performed. Stratum corneum of the graft got stripped off in two to four weeks after pulp graft. The color of the pulp graft was slightly reddish, then it became similar to the adjacent tissue. Results: There was complete take in all the patients who were treated with pulp graft. Great toe pulp graft provided similar color and texture to the adjacent skin, high rate of graft take, and left only a minimal scar at donor site. Conclusion: Thick keratin layer and inelastic nature of the pulp make this type of the graft much easier and simpler, and ensure a better take. Pulp graft is useful method for the reconstruction of the postburn flexion contracture in fingers.

• Journal of Photoscience
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• 제9권2호
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• pp.225-228
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• 2002

Bcl-2 is a member of large bcl-2 family and protects cells from apoptosis. Using bcl-2-expressing adenovirus vector (Ad-bc1-2), we investigated the effect of bc1-2 on UVB-induced apoptosis. Adenovirus vector efficiently introduced bc1-2 gene in cultured normal mouse keratinocytes (NMK cells); almost all NMK cells (lx10$^{6}$ ) were transfected at Ixl0$^{8}$ PFU/ml. Bcl-2-transfected NMK cells were significantly resistant to UVB-induced apoptosis with the suppressive effect dependent on bcl-2-expression level. Following UVB irradiation caspase 8, 3, 9 activities were stimulated in NMK cells, while in bc1-2-transfected cells, only caspase 8, but not caspase 3 or 9 activities were stimulated. In order to investigate the effect of bc1-2 in vivo, topical application of Ad-bc1-2 on tape-stripped mouse skin was performed. Following the application, Bc1-2 was efficiently overexpressed in almost all viable keratinocytes. The expression was transient with the maximal expression of Bc1-2 at 1st day following the application of lxl0$^{9}$ PFU in 200ml. The introduced Bc1-2 remained at least for 6 days. UVB irradiation (1250 J/m$^2$) induced apoptosis within 12 h and the maximal effect was observed at 24 h in control mouse skin. Bc1-2-transfected mice skin were resistant to UVB-induced apoptosis. Topical application of empty adenovirus vector alone had no effect on Bc1-2 expression or UVB-induced apoptosis. These results indicate that adenovirus vector is an efficient gene delivery system into keratinocytes and that Bcl-2 is a potent inhibitor of UVB-induced apoptosis both in vitro and in vivo.

• 대한화장품학회지
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• 제44권1호
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• pp.81-87
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• 2018

본 연구에서는 각질층의 산란에 대한 새로운 분석 방법을 제공하고 광학에 사용되는 광산란 감소 물질들의 피부 각질층에서의 영향을 확인하였다. 각질층과 피부 구성 성분에 의해 산란되어지는 빛의 변화를 측정하기 위해 각질 테이프로 분리한 각질세포의 다크 필드 이미지 분석을 통해 다양한 광산란 감소 물질들의 각질층에서의 광산란 효과를 측정하고 분석하였다. 광산란 감소 물질의 처리 후 각질층의 물리적 특성(수분 함량, 케라틴 구조 및 두께) 변화는 FT-IR, 3D 레이저 현미경으로 관찰하였는데 화장품에서 보습제로 사용되는 단당류, 당 알코올, 환원당, 히알루론산 등은 광산란을 감소시킬 수 있었다. 그러나 광학에서의 탈수 현상과 달리 낮은 농도조건의 광산란 감소 물질은 각질층 케라틴 구조의 내부로 물의 침투를 향상시켜 각질층의 부피를 증가시키고 강성을 감소시키는 효과를 나타내었다. 이러한 광산란 감소 물질에 의한 각질층 각질세포의 수화 현상은 각질층의 광산란을 감소시켜 피부를 보다 투명하게 보이게 해 줄뿐만 아니라 지속적인 보습효과를 제공할 수 있는 각질층 타겟의 새로운 피부 개선 연구의 가능성을 확인할 수 있었다.

• Nutrition Research and Practice
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• 제10권4호
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• pp.398-403
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• 2016

BACKGROUND/OBJECTIVES: Atopic dermatitis (AD), a chronic inflammatory skin disease, is accompanied by disruption of the epidermal lipid barrier, of which ceramide (Cer) is the major component. Recently it was reported that vitamin C is essential for de novo synthesis of Cer in the epidermis and that the level of vitamin C in plasma is decreased in AD. The objective of this study was to determine the associations among clinical severity, vitamin C in either plasma or epidermis, and Cer in the epidermis of patients with AD. SUBJECTS/METHODS: A total of 17 patients (11 male and 6 female) aged 20-42 years were enrolled. The clinical severity of AD was assessed according to the SCORAD (SCORing Atopic Dermatitis) system. Levels of vitamin C were determined in plasma and biopsies of lesional epidermis. Levels of epidermal lipids, including Cer, were determined from tape-stripped lesional epidermis. RESULTS: The clinical severity of patients ranged between 0.1 and 45 (mild to severe AD) based on the SCORAD system. As the SCORAD score increased, the level of vitamin C in the plasma, but not in the epidermis, decreased, and levels of total Cer and Cer2, the major Cer species in the epidermis, also decreased. There was also a positive association between level of vitamin C in the plasma and level of total Cer in the epidermis. However, levels of epidermal total lipids including triglyceride, cholesterol, and free fatty acid (FFA) were not associated with either SCORAD score or level of vitamin C in the plasma of all subjects. CONCLUSIONS: As the clinical severity of AD increased, level of vitamin C in the plasma and level of epidermal Cer decreased, and there was a positive association between these two parameters, implying associations among plasma vitamin C, epidermal Cer, and the clinical severity of AD.

• 심성연구
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• 제32권2호
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• pp.122-168
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• 2017

한국 민담 <외쪽이>를 분석심리학의 관점에서 해석하였다. <외쪽이> 민담은 전국적으로 분포되어 있으며, 인도와 아프리카에 유화가 있다. 대상 민담을 보면 아이를 낳지 못하는 부인이 수태 음식 두 개 반을 먹고 온전한 아들 두 명과 팔도 하나, 다리도 하나, 눈도 하나인 반쪽 아들을 낳았다. 형들은 과거 보러갈 때 외쪽이가 따라오는 것을 싫어해서 바위와 나무에 묶어 놓지만, 외쪽이는 힘이 세서 쑥 뽑아서 집의 뜰에 가져다 놓았다. 형들은 외쪽이를 칡으로 묶어서 호랑이 앞에 던져 놓지만 외쪽이는 호랑이와 칡 끊기 내기에서 이겨 호랑이 가죽을 벗겨 돌아온다. 호랑이 가죽을 탐낸 숙소 주인과 장기 내기에서 이겨 주인의 딸을 데려오기로 하고 노끈과 북, 벼룩, 빈대를 가지고 가서 주인집 사람들을 곯려주고 처녀를 데려와서 잘 살았다는 이야기이다. 아이를 낳지 못하는 부인이 부처에게 비는 제시부는 창조성이 고갈된 의식의 한계 상황에서 의식의 자아가 무의식에 겸허한 태도를 취하며 정신의 자발적 활동을 기다리는 태도이다. 하얀 영감이 와서 고기 세 마리를 주는데 고양이가 와서 반 마리를 먹어서 부인은 고기를 두 마리와 반 마리만 먹고 온전한 아들 둘과 막내 아들 외쪽이를 낳는다. 노현자 상으로 나타난 정신(Geist)과 고양이로 나타난 동물적 본성이 작용하여 무의식에서 낯설고 새로운 형상을 지닌 정신적 내용이 생성되었다. 반쪽 존재는 전 세계적으로 나타나는 원형상으로 많은 문화에서 신과 정령이 반쪽만 있는 모습으로 그려진다. 창조 신화에서 반쪽 존재는 생식 능력이 없지만, 죽지 않고 불멸한다고 전해진다. 인도네시아와 아프리카의 설화에서는 반쪽으로 태어난 소년이 하늘나라에 가서 자신의 반쪽을 만나 합쳐져서 온전한 몸이 된다. 반쪽 정령이 인간에게 해로운 경우도 있지만, 반쪽만 있는 새, 닭, 정령이 인간에게 도움을 주는 이야기도 있다. 반쪽 존재는 하늘에서 곡식을 훔쳐 오거나 대나무를 다루는 방법을 배워 오는 문화영웅이다. 반쪽 존재가 나중에 온전한 몸이 되는 이야기도 있지만, 대상 민담과 대부분의 유화에서는 이야기의 결말까지 반쪽 형상이 그대로 유지된다. 외쪽이는 무의식에서 태어난 정신적 체험의 상징이다. 형들이 외쪽이를 싫어하는 것에서 보이듯이 무의식의 내용은 처음에는 이상하고 기괴하게 보일 수 있으며 집단적 의식은 무의식의 내용을 받아들이지 않으려 하지만 새로운 정신은 영향력을 행사하며 의식의 태도를 변화시킬 수 있다. 외쪽이는 호랑이를 퇴치하고 호랑이 가죽을 벗겨 오는데 호랑이는 집어 삼키려는 무의식이며 집단적 무의식의 부정적 모성 원형으로 무의식을 의식화하는 과정에서 극복해야 할 괴물이다. 숙소 주인의 딸을 데려올 때 외쪽이는 떨어져 있는 것을 연결해 주는 끈과 음악과 무속에 쓰이는 악기, 하찮아 보이는 벌레를 들고 가서 점잖은 척 하는 사람들을 놀려주는 동시에 활기와 창조성을 가져다준다. 외쪽이는 트릭스터로서 유교적 신분 사회였던 우리 사회의 집단적 의식을 보상하는 역할을 했다고 보여진다. 외쪽이는 비정상적인 것으로 보이지만 우리가 잘 알지 못하는 세상 혹은 존재와 연결되어 있고, 어려움이 있을 때 도움을 주며 그전에 없던 것을 가져다 주는 문화 영웅이기도 한 정신적 내용으로 집단적 무의식에 존재하며 점차 드러날 심성이다.