• Coupled systems mechanics
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• v.11 no.6
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• pp.557-586
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• 2022

In spite of extensive testing of the individual shear wall and the coupling beam (CB), numerical and experimental researches on the seismic behavior of CSW are insufficient. As far as we know, no previous research has investigated the affectations of position of CB regarding to the slab level (SL). So, the investigation aims to enhance an overarching framework to examine the consequence of connection positions between CB and SL. And, three cases have been created. One is composed of the floor slab (FS) at the top of the CB (FSTCB); the second is created with the FS within the panel depth (FSWCB), and the third is employed with the FS at the bottom of the CB (FSLCB). And, FEA is used to demonstrate the consequences of various CB positions with regard to the SL. Furthermore, the main measurements of structure response that have been investigated are deformation, shear, and moment in a coupled beam. Additionally, wall elements are used to simulate CB. In addition, ABAQUS software was used to figure out the strain distribution, shear stress for four stories to further understand the implications of slab position cases on the coupled beam rigidity. Overall, the findings show that the position of the rigid linkage among the CB and the FS can affect the behavior of the structures under seismic loads. For all structural heights (4, 8, 12 stories), the straining actions in FSWCB and FSLCB were less than those in FSTCB. And, the increases in displacement time history response for FSWCB are around 16.1-81.8%, 31.4-34.7%, and 17.5% of FSTCB.

• Research in Plant Disease
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• v.29 no.3
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• pp.321-329
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• 2023

The bacterium Neobacillus endophyticus BRMEA1^T, isolated from the medicinal plant Selaginella involvens, known as its thermotolerant can grow at 50℃. To explore the genetic basis for its heat tolerance response and its potential for producing valuable natural compounds, the genomes of two thermotolerant and four mesophilic strains in the genus Neobacillus were analyzed using a bioinformatic software platform. The whole genome was annotated using RAST SEED and OrthVenn2, with a focus on identifying potential heattolerance-related genes. N. endophyticus BRMEA1^T was found to possess more stress response genes compared to other mesophilic members of the genus, and it was the only strain that had genes for the synthesis of osmoregulated periplasmic glucans. This study sheds light on the potential value of N. endophyticus BRMEA1^T, as it reveals the mechanism of heat resistance and the application of secondary metabolites produced by this bacterium through whole-genome sequencing and comparative analysis.

• Proceedings of the Microbiological Society of Korea Conference
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• 2007.05a
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• pp.120-122
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• 2007

All living organisms use numerous signal-transduction pathways to sense and respond to their environments and thereby survive and proliferate in a range of biological niches. Molecular dissection of these signalling networks has increased our understanding of these communication processes and provides a platform for therapeutic intervention when these pathways malfunction in disease states, including infection. Owing to the expanding availability of sequenced genomes, a wealth of genetic and molecular tools and the conservation of signalling networks, members of the fungal kingdom serve as excellent model systems for more complex, multicellular organisms. Here, we employed Cryptococcus neoformans as a model system to understand how fungal-signalling circuits operate at the molecular level to sense and respond to a plethora of environmental stresses, including osmoticshock, UV, high temperature, oxidative stress and toxic drugs/metabolites. The stress-activated p38/Hog1 MAPK pathway is structurally conserved in many organisms as diverse as yeast and mammals, but its regulation is uniquely specialized in a majority of clinical Cryptococcus neoformans serotype A and D strains to control differentiation and virulence factor regulation. C. neoformans Hog1 MAPK is controlled by Pbs2 MAPK kinase (MAPKK). The Pbs2-Hog1 MAPK cascade is controlled by the fungal "two-component" system that is composed of a response regulator, Ssk1, and multiple sensor kinases, including two-component.like (Tco) 1 and Tco2. Tco1 and Tco2 play shared and distinct roles in stress responses and drug sensitivity through the Hog1 MAPK system. Furthermore, each sensor kinase mediates unique cellular functions for virulence and morphological differentiation. We also identified and characterized the Ssk2 MAPKKK upstream of the MAPKK Pbs2 and the MAPK Hog1 in C. neoformans. The SSK2 gene was identified as a potential component responsible for differential Hog1 regulation between the serotype D sibling f1 strains B3501 and B3502 through comparative analysis of their meiotic map with the meiotic segregation of Hog1-dependent sensitivity to the fungicide fludioxonil. Ssk2 is the only polymorphic component in the Hog1 MAPK module, including two coding sequence changes between the SSK2 alleles in B3501 and B3502 strains. To further support this finding, the SSK2 allele exchange completely swapped Hog1-related phenotypes between B3501 and B3502 strains. In the serotype A strain H99, disruption of the SSK2 gene dramatically enhanced capsule biosynthesis and mating efficiency, similar to pbs2 and hog1 mutations. Furthermore, ssk2, pbs2, and hog1 mutants are all hypersensitive to a variety of stresses and completely resistant to fludioxonil. Taken together, these findings indicate that Ssk2 is the critical interface protein connecting the two-component system and the Pbs2-Hog1 pathway in C. neoformans.

• Restorative Dentistry and Endodontics
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• v.44 no.3
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• pp.33.1-33.12
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• 2019

Objectives: To evaluate the influence of the restorative technique on the mechanical response of endodontically-treated upper premolars with mesio-occluso-distal (MOD) cavity. Materials and Methods: Forty-eight premolars received MOD preparation (4 groups, n = 12) with different restorative techniques: glass ionomer cement + composite resin (the GIC group), a metallic post + composite resin (the MP group), a fiberglass post + composite resin (the FGP group), or no endodontic treatment + restoration with composite resin (the CR group). Cusp strain and load-bearing capacity were evaluated. One-way analysis of variance and the Tukey test were used with ${\alpha}=5%$. Finite element analysis (FEA) was used to calculate displacement and tensile stress for the teeth and restorations. Results: MP showed the highest cusp (p = 0.027) deflection ($24.28{\pm}5.09{\mu}m/{\mu}m$), followed by FGP ($20.61{\pm}5.05{\mu}m/{\mu}m$), CR ($17.62{\pm}7.00{\mu}m/{\mu}m$), and GIC ($17.62{\pm}7.00{\mu}m/{\mu}m$). For load-bearing, CR ($38.89{\pm}3.24N$) showed the highest, followed by GIC ($37.51{\pm}6.69N$), FGP ($29.80{\pm}10.03N$), and MP ($18.41{\pm}4.15N$) (p = 0.001) value. FEA showed similar behavior in the restorations in all groups, while MP showed the highest stress concentration in the tooth and post. Conclusions: There is no mechanical advantage in using intraradicular posts for endodontically-treated premolars requiring MOD restoration. Filling the pulp chamber with GIC and restoring the tooth with only CR showed the most promising results for cusp deflection, failure load, and stress distribution.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.14 no.1
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• pp.436-444
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• 2013

This article presents the dynamic response of nano-scale plates using the nonlocal continuum theory and higher-order shear deformation theory. The nonlocal elasticity of Eringen has ability to capture the small scale effects and the higher-order shear deformation theory has ability to capture the quadratic variation of shear strain and consequently shear stress through the plate thickness. The solutions of transient dynamic analysis of nano-scale plate are presented using these theories to illustrate the effect of nonlocal theory on dynamic response of the nano-scale plates. The relations between nonlocal and local theories are discussed by numerical results. Also, the effects of nonlocal parameters, aspect ratio, side-to-thickness ratio, size of nano-scale plate and time step on dynamic response are investigated and discussed. The amplitude and cycle increase when nonlocal parameter increase. In order to validate the present solutions, the reference solutions are used and discussed. The theoretical development as well as numerical solutions presented herein should serve as reference for nonlocal theories as applied to the transient dynamic analysis of nano-scale structures.

• Proceedings of the Korean Society for Applied Microbiology Conference
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• 2005.06a
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• pp.154-164
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• 2005

Saccharomyces cerevisiae KNU5377 is a thermotolerant strain, which can ferment ethanol from wasted papers and starch at 40$^{\circ}C$ with the almost same rate as at 30$^{\circ}C$. This strain showed alcohol fermentation ability to convert wasted papers 200 g (w/v) to ethanol 8.4% (v/v) at 40$^{\circ}C$, meaning that 8.4% ethanol is acceptable enough to ferment in the industrial economy. As well, all kinds of starch that are using in the industry were converted into ethanol at 40$^{\circ}C$ with the almost same rate as at 30$^{\circ}C$. Hyperthermic cell killing kinetics and differential scanning calorimetry (DSC) revealed that exponentially growing cells of this yeast strain KNU5377 were more thermotolerant than those of S. cerevisiae ATCC24858 used as a control. This intrinsic thermotolernace did not result from the stability of entire cellular components but possibly from that of a particular target. Heat shock induced similar results in whole cell DSC profiles of both strains and the accumulation of trehalose in the cells of both strains, but the trehalose contents in the strain KNU5377 were 2.6 fold higher than that in the control strain. On the contrary to the trehalose level, the neutral trehalase activity in the KNU5377 cells was not changed after the heat shock. This result made a conclusion that though the trehalose may stabilize cellular components, the surplus of trehalose in KNU5377 strain was not essential for stabilization of whole cellular components. A constitutively thermotolerant yeast, S. cerevisiae KNU5377, was compared with a relatively thermosensitive control, S. cerevisiae ATCC24858, by assaying the fluidity and proton ATPase on the plasma membrane. Anisotropic values (r) of both strains were slightly increased by elevating the incubation temperatures from 25$^{\circ}C$ to 37$^{\circ}C$ when they were aerobically cultured for 12 hours in the YPD media, implying the membrane fluidity was decreased. While the temperature was elevated up to 40$^{\circ}C$, the fluidity was not changed in the KNU5377 cell, but rather increased in the control. This result implies that the plasma membrane of the KNU5377 cell can be characterized into the more stabilized state than control. Besides, heat shock decreased the fluidity in the control strain, but not in the KNU5377 strain. This means also there's a stabilization of the plasma membrane in the KNU5377 cell. Furthermore, the proton ATPase assay indicated the KNU5377 cell kept a relatively more stabilized glucose metabolism at high temperature than the control cell. Therefore, the results were concluded that the stabilization of plasma membrane and growth at high temperature for the KNU5377 cell. Genome wide transcription analysis showed that the heat shock responses were very complex and combinatory in the KNU5377 cell. Induced by the heat shock, a number of genes were related with the ubiquitin mediated proteolysis, metallothionein (prevent ROS production from copper), hsp27 (88-fold induced remarkably, preventing the protein aggregation and denaturation), oxidative stress response (to remove the hydrogen peroxide), and etc.

• Journal of the Korea Concrete Institute
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• v.14 no.6
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• pp.892-899
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• 2002

Among the methods for enhancement of load-carrying capacity on flexural concrete member, recently, a concept is being investigated which replaces the steel in a conventional reinforced concrete member with a fiber reinforced polymer(FRP) shell. This study focuses on modeling of the structural behavior of concrete surrounded with FRP shells in flexural bending members. A numerical model of fiber cross-sectional analysis is proposed to predict the stress and deformation state of the FRP shell and concrete. The stress-strain relationship of concrete confined by a FRP shell is formulated to be based on the constitutive law of concrete in multi-axial compressive stress state, in assuming that the compression response is dependent on the radial expansion of the concrete. To describe the FRP shell behavior, equivalent orthotropic properties of in-plane behavior from classical lamination theory are used. The present model is validated to compare with the experiments of 4-point bending tests of FRP shell concrete beam, and has well predicted the moment-curvature relationships of the members, axial and hoop strains in the section, and the enhancement of confinement effect in concrete surrounded by FRP shell.

• Journal of the Korean Geotechnical Society
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• v.35 no.4
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• pp.15-26
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• 2019

The stability of the gravity quay wall against earthquakes is evaluated on the basis of the allowable displacement of the wall. To estimate the displacement caused by external forces, empirical equations based on the Newmark sliding block method or numerical analysis are widely used. In numerical analysis, it is possible to analyze precisely a complicated site and structure, but difficult to set the appropriate parameters and environments; there are limitations in obtaining reliable results, depending on one's level of expertise. The Newmark method, with only seismic motions, is widely used because it is simpler than numerical simulations when estimating permanent displacement. However, the empirical equations do not have any parameters for the response characteristics and sliding block of the structure, and sliding blocks being assumed as rigid bodies does not consider the nonlinear behavior of the soil and interaction with the structure. Therefore, in order to evaluate the seismic stability of the gravity quay wall, a newly-developed empirical equation is needed to overcome the above-mentioned limitations. In this study, numerical simulations are performed to analyze the response characteristics of the backfill of the structure, and to propose an optimal method of calculating the active area. For this purpose, finite element analyses were performed to analyze the response characteristics, and stress-strain relationships for various seismic motions. As a result, the response characteristics, sliding block, and failure surface of the backfill vary depending on the input seismic motions.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.30 no.6C
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• pp.255-263
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• 2010

This paper investigates the effects of tunnelling-induced ground movements on nearby structures, considering soil-structure interactions of different construction (ground loss) and soil characteristics. The response of four-story block structures, which are subjected to tunnelling-induced ground movements, has been investigated in different construction (ground loss) and soil conditions using numerical analysis. The structures for numerical analysis has been modelled using Discrete Element Method (DEM) to have real cracks when the shear and tensile stress exceed the maximum shear and tensile strength. The response of four-story block structures has been investigated with a ground movement magnitude and compared in terms of construction (ground loss) and soil conditions considering the magnitude of deformations and cracks in structures. In addition, the damage levels, which are possibly induced in structures, has been provided in terms of construction (ground loss) and soil conditions using the state of strain damage estimation criterion (Son and Cording, 2005). The results of this study will provide a background for better understandings for controlling and minimizing building damage on nearby structures due to tunnelling-induced ground movements.

• Journal of dental hygiene science
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• v.9 no.3
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• pp.361-367
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• 2009

This study was conducted to know oral maxillofacial symptoms accroding to dental fear and dental use behavior. 334 persons Older than junior high school students in Daegu, South Korea who visited to dental clinic were targeted From March 1.2008 to March 15, 2008. Statistics tool was SPSS 12.0 for Windows. General characteristics and response degree according to oral maxillofacial symptoms was analyzed by frequency study. Fear and difference according to oral maxillofacial symptoms we re conducted by T-test and One-Way ANOVA. Correlation analysis was used to verify relation between fear and oral maxillofacial symptoms. Results are followings. 1. Women felt fear higher than men did. Persons who were older felt fear higher and these were statistically related. 2. Extraoral external was 62.9%, hightest on the symptoms response degree according to oral maxillofacial symptoms. Feeling back side of the head on the strain and neck was stiff on the items were high. 3. Oral maxillofacial symptoms were high in the case when they had regular inspection, experience of scaling, experience of anesthesia and visited to the clinic often. And. Two cases(having regular inspection and visiting to the clinic often) were statistically related. Conclusion: Each pan of oral maxillofacial symptoms in the high fear group according to oral maxillofacial symptoms and it was all statistically related. And, fear and each oral maxillofacial pains were positively related and it was statistically related.