• Journal of Korean Society of Steel Construction
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• v.15 no.5 s.66
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• pp.579-589
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• 2003

Most beam-to-column connections are symmetrically reinforced because of the reverse action caused by earthquakes. However, in weak-earthquake regions like Korea, asymmetrically reinforced connections could be used. In particular, the connections between concrete-filled tube (CFT) column and H-shape beam could be applied using a simplified lower diaphragm. The tensile capacity or Combined Cross Diaphragm for upper reinforcing was tested using a simple tension test. Four types for lower reinforcing combined Cross, none, horizontal T-bar, and vertical plate were tested using the ANSI/AISC SSPEC 2002 loading program. Horizontal T-bar and stud bolts in vertical flat, bar transmit tensile stress from the beam's bottom flange to filled concrete. All test specimens satisfied 0.01 radian inelastic rotational requirement in ordinary moment frame of AISC seismic provision. According to the results of the parametric studies simplified lower diaphragms demonstrated outstanding strength, stiffness, and plastic deformation capacity which could lead to more sufficient seismic performance in the field.

• Journal of the Korea institute for structural maintenance and inspection
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• v.14 no.4
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• pp.70-77
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• 2010

Microcement grouting and micro pile are frequently used for ground modification during tunnel construction. The influence of grouting pressure of microcement grouting and micro pile to the existing bridge which is directly over the constructing tunnel is investigated. Three dimensional seepage flow-structure interactive analysis considering firm water pressure with full stages of construction including the construction of upper bridge, microcement grouting, micro pile and tunnel is performed. The settlement and tilting of the pier of existing bridge violate the design code and the reaction of the bridge are highly increased after grouting. The stress of tunnel bracings such as rockbolt and shotcrete also exceed the limit of the code. The pressure of microcement grouting is confined by bedrock and transmit to the surrounded soil and the upper bridge. Microcement grouting needs mid-high pressure to penetrate through weak fault plane and the pressure greatly influence the safety of the upper structure. It is important to decide and care the grouting pressure to improve weak fault plane directly under the existing structures and the pressure of microcement grouting should be considered in underground analysis.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.20 no.3
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• pp.269-275
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• 2019

As the national industry is developing gradually due to the expansion of the economic scale, the construction of large and super high-rise structures for building social infrastructure has been increasing, and studies have been conducted actively to transmit the large loads at the upper portion to the lower bedrock. In this study, the PHC was extended to an ultra-high strength PHC, which increased the concrete compressive strength of the PHC from the conventional 80 MPa to 110 MPa, and the PHC, which extended the tip of the pile. After construction with the driving method and injected pile method, the tendency of the bearing capacity was tested through a load test. Measurements of the bearing capacity of the extended PHC using the pile driving method revealed the main surface friction force to be smaller than that of the general PHC, and the stet-up effect was also insignificant. On the other hand, the effect of the friction force on the ground surface when the injected pile method was applied is expected to increase the bearing capacity when the gap between the main surface and the ground is wide and the cement paste is filled tightly. In addition, the ultrahigh strength PHC showed higher bearing capacity than the conventional PHC, and the permissible pile stress was less than 60%. Therefore, it is possible to reduce the number of piles and reduce the construction cost and effect of shortening the length of the pile by designing the tip of the pile on the ground with the intensity of soft rock as a method for utilizing the increased strength of the ultra-high strength PHC.

• Journal of Life Science
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• v.30 no.2
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• pp.137-146
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• 2020

Calcium is one of the important secondary signaling molecules in plant cells. Calcium-dependent protein kinases (CDPK)-the sensor proteins of Ca²⁺ and phosphorylating enzymes-are the most abundant serine/threonine kinases in plant cells. They convert and transmit signals in response to various stimuli, resulting in specific responses in plants. In rice, 31 CDPK gene families have been identified, which are mainly involved in plant growth and development and are known to play roles in response to various stress conditions. However, little is known about the biochemical characteristics of CDPK proteins. In this study, OsCPK11-a CDPK in rice-was partially purified, and its biochemical characteristics were found. Partially purified OsCPK11 from rice seedlings was obtained by three-step column chromatography that involved anion exchange chromatography consisting of DEAE, hydrophobic interaction chromatography consisting of phenyl-Sepharose, and gel filtration chromatography consisting of Sephacryl-200HR. An in vitro kinase assay using partially purified OsCPK11 was also performed. This partially purified OsCPK11 had a molecular weight of 54 kDa and showed a strong hydrophobic interaction with the hydrophobic resin. In vitro kinase assay showed that the OsCPK11 also had Ca²⁺-dependent autophosphorylation activity. The OsCPK11 phosphorylated histone III-S, and the optimum pH for its kinase activity was found to be 7.5~8.0. The native OsCPK11 shared several biochemical characteristics with recombinant OsCPK11 studied previously, and both had Ca²⁺-dependent autophosphorylation activity and favored histone III-S as a substrate for kinase activity, which also had a Ca²⁺-dependence.