• Plant Biotechnology Reports
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• 제2권2호
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• pp.123-131
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• 2008

Vitrification methods are convenient for cryopreserving plant specimens, as the specimens are plunged directly into liquid nitrogen (LN) from ambient temperatures. However, tissues and species with poor survival are still not uncommon. The development of vitrification solutions with high survival that cover a range of materials is important. We attempted to develop new vitrification solutions using bromegrass cells and found that VSL, comprising 20% (w/v) glycerol, 30% (w/v) ethylene glycol, 5% (w/v) sucrose, 10% (w/v) DMSO and 10 mM $CaCl_2$, gave the highest survival following cryopreservation, as determined by fluorescein diacetate staining. However, the cryopreserved cells showed little regrowth, for unknown reasons. To check its applicability, VSL was used to cryopreserve gentian axillary buds and the performance was compared with those of conventional vitrification solutions. Excised gentian stem segments with axillary buds (shoot apices) were two-step precultured with sucrose to induce osmotic tolerance prior to cryopreservation. Gentian axillary buds cryopreserved using VSL following the appropriate preculturing approach exhibited 78% survival (determined by the regrowth capacity), which was comparable to PVS2 and PVS1 and far better than PVS3. VSL had a wider optimal incubation time (20-45 min) than PVS2 and was more suitable for cryopreserving gentian buds. The optimal duration of the first step of the preculture was 7-11 days, and preculturing with sucrose and glucose gave a much higher survival than fructose and maltose. VSL was able to vitrify during cooling to LN temperatures, as glass transition and devitrification points were detected in the warming profiles from differential scanning calorimetry. VSL and its derivative, VSL+, seem to have the potential to be good alternatives to PVS2 for the cryopreservation of some materials, as exemplified by gentian buds.

• 한국지반공학회논문집
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• 제15권3호
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• pp.3-16
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• 1999

본 연구에서 미관통과 관통 샌드컴팩션파일(SCP) 개량지반에 대하여 일차원 압밀거동을 조사하기 위하여 실내모형실험을 실시하였다. 모델점토지반에 간극수압계, 토압계, 다이얼게이지등을 설치하여 SCP 개량지반의 압밀침하량과 응력분담비등을 측정하였다. 실험 결과, 미관통 SCP 지반의 압밀침하량은 관통 SCP 지반의 압밀침하량 보다 더 크게, 응력분담비는 더 적게 나타났다. 또한 SCP 개량지반의 응력분담비는 압밀시간, 지반심도, 상대밀도, 치환율과 관통률에 따라 변화됨을 알 수 있었다.

• 대한간호학회지
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• 제35권4호
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• pp.672-679
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• 2005

Introduction. Cardiovascular disease has the potential to lead to sudden in-flight incapacitation and permanent grounding. The aims of this study are to examine the relationships between lifestyle, job stress and blood lipid levels of male aircrew personnel of a Korean airline and to identify which factors influence their hyper-lipidemia. Methods. Two hundred sixteen male aircrew personnel completed a questionnaire by self-report and consented to participate in the study. The questionnaire collected data related to job stress, life style, serum cholesterol levels and general characteristics of the aircrew. The cholesterol levels of the subjects were collected from their most recent health check-up records. Subjects were divided into two groups (the desirable group and the risk group) based on their serum cholesterol level, 200 mg/dl. Results. Mean age and marital status were significantly different between the two groups. More subjects in the risk group had habits of eating high lipid foods, while more subjects in the desirable group exercised more frequently than the risk group. In logistic regression analysis, after controlling age and marital status, types of working situation (domestic duty or international duty, odds ratio=.390, p=.018), diet (odds ratio=.429, p=.037), and exercise (odds ratio=.320, p=.055) were influencing factors on aircrew's serum cholesterol lev-els. Conclusions. The cholesterol level of aircrew personnel is closely related to their lifestyle, such as lipid diet and exercise. The type of work situations, e.g. staying in an airplane for long periods of time or staying abroad, may influence these diet patterns and exercise habits.

• Child Health Nursing Research
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• 제17권1호
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• pp.39-47
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• 2011

Purpose: This study was done to determine whether a parenting education program using responsive teaching strategies is effective for parenting stress, maternal interactional behavior and behavioral problems in children at risk for Attention Deficit Hyperactivity Disorder (ADHD). Methods: Participants in this study were 17 elementary school children and their parents. The ADHD risk group was determined by scores on the Korean-ADHD Rating Scale. The parenting education program was developed based on Dr. Mahoney's responsive teaching curriculum. Mothers participated in the program once a week for 8 sessions. Treatment outcome was evaluated using the Korean version of the Child Behavior CheckList (K-CBCL), parenting stress, maternal interactional behavior, and child pivotal developmental behavior. Data were analyzed using Wilcoxon signed rank test, Mann-Whitney test, and regression analysis. Results: After the parenting education program, no significant difference in parenting stress (Z=-1.00, p=.320) was found, but there was a significant decrease in the child's internal behavior problems (Z=-2.05, p=.040), and also a significant improvement in maternal interactional behavior and a significant difference in child pivotal developmental behavior (Z=-2.67, p=.008). Conclusion: The results indicate that parenting education programs based on responsive teaching strategies are effective and that application of a program is recommended to prevent behavioral problems and improve maternal child interaction for children at risk for ADHD.

• 터널과지하공간
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• 제17권2호
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• pp.90-101
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• 2007

최근 지하공간개발의 증가와 한께 상부저수지에 저장된 물을 하부저수지고 낙하시켜 발전하는 지하양수 발전소의 건설도 지속적으로 이루어지고 있다. 이거한 지하양수발전소를 건설할 때, 구조물의 효율적이고 안전한 설계 시공 및 유지를 위해서 설계단계에서 암석과 암반의 공학적 특성 및 대상지역의 지압상태 등을 명확히 파악하는 것은 매우 중요하다. 본 논문에서 소개하는 양수발전소는 심포 $320{\sim}375m$에서 건설되고 있으며, 구조물의 안정성 검토를 위하여 굴착예정지 암반에 대해 오버코어링법을 이용한 초기응력측정, 공내재하시험 및 암석의 물성시험을 실시하여 설계에 필요한 기초자료를 획득하였다. 이를 바탕으로 수치해석을 실시하여 기존 지보재 설계의 적정성을 판단하였고, 불안정성으로 판정된 부분에 대해서는 추가로 보강설계를 하였다. 추가 보강설계 결과, 공동 전체에서 안정성을 확보하였고 현재 순조롭게 굴착이 이루어지고 있는 상태이다.

• 한국터널지하공간학회 논문집
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• 제14권2호
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• pp.131-139
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• 2012

본 연구에서는 도심지 소구경 터널굴착공법에서 파형강판으로 제작된 liner plate의 거동특성에 대해 알아보기 위해 유한요소 해석 프로그램인 MIDAS/GTS를 이용하여 수치해석을 실시하였다. 토피고 변화에 따라 liner plate의 거동특성을 알아보기 위해 토피고는 5 m, 10 m, 15 m, 20 m, 25 m 5가지 각 조건으로 해석을 실시하였다. 수치해석결과 liner plate의 최대변위와 최대축응력은 부재의 하단부에서 발생되며, 최대전단응력의 경우 부재의 천단부에서 발생되는 것으로 확인되었다. 또한 토피고에 따른 liner plate의 변위, 응력, 전단응력의 변화는 있었으나 그 차이가 미미하고 토피고가 증가함에 따라 점점 구조적 안정성이 확보되는 것으로 판단된다.

• Psychiatry investigation
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• 제15권11호
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• pp.1071-1078
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• 2018

Objective We developed easily accessible imagery-based treatment program for patients with post-traumatic stress disorder (PTSD) related to workplace accidents and investigated the effects of the program on various PTSD related symptoms. Methods The program was based on an online platform and consisted of eight 15-min sessions that included script-guided imagery and supportive music. Thirty-five patients with workplace-related PTSD participated in this program 4 days per week for 4 weeks. Its effects were examined using self-report questionnaires before and after the take-home online treatment sessions. Results After completing the 4-week treatment program, patients showed significant improvements in depressed mood (t=3.642, p=0.001) based on the Patient Health Questionnaire-9 (PHQ-9), anxiety (t=3.198, p=0.003) based on the Generalized Anxiety Disorder seven-item (GAD-7) scale, and PTSD symptoms (t=5.363, p<0.001) based on the Posttraumatic Stress Disorder Check List (PCL). In particular, patients with adverse childhood experiences exhibited a greater degree of relief related to anxiety and PTSD symptoms than those without adverse childhood experiences. Conclusion The present results demonstrated that the relatively short online imagery-based treatment program developed for this study had beneficial effects for patients with workplace-related PTSD.

• Physical Therapy Rehabilitation Science
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• 제11권4호
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• pp.565-570
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• 2022

Objective: The purpose of this study was to investigate the correlation between activity of daily living and social psychology and work mind of office workers with non-specific chronic neck pain (NSCNP). Design: Crossed-sectional study Methods: 86 patients with NSCNP were recruited for this study. Neck disability index (NDI) and Numerical pain rating scale (NPRS) were used to check the pain intensity and disability of patients with neck pain. To find out the occupational factors of the subjects, the Korean version of Latack Coping Scale was used. And,To find out the socio-psychological factors of the subjects, the Korean version of depression anxiety stress scale (DASS-21) was used. We performed correlation for each variable. Results: The correlation between NPRS and NDI and DASS-21 Scale were clear positive correlation (p<0.05). There was no statistical significance between the LATACK control group and the pain and disability index (p>0.05). A clear positive correlation was established between the avoidance group of LATACK and pain (p<0.01). Conclusions: Through this study, it is necessary to have time to manage depression, anxiety, and stress in the treatment of neck pain in office workers who spend a lot of time sitting. In addition, it is thought that there should be the ability to control oneself about one's duties in the workplace.

• 영어영문학
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• 제56권5호
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• pp.851-870
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• 2010

The purpose of this paper is to investigate the distribution of /ju/ in British English, especially after the coronal sonorants /n, l, /r/. The sequence /ju/ is related with vowels such as /u/, /ʊ/, and /ʊ/, and has occasioned a variety of conflicting analyses or suggestions. One of those is in which context /j/ is deleted if we suppose that the underlying form is /ju/. The context differs according to the dialect we deal with. In British English, it is known that /j/ is deleted always after /r/, and usually after /l/ when it occurs in an unstressed word-medial syllable. To check this well-known fact I searched OED Online (the 2nd Edition, 1989) for those words which contain /n, l, r/ + /ju, jʊ, u, ʊ, (j)u, (j)ʊ/ in their pronunciations, using the search engine provided by OED Online. After removing some unnecessary words, I classified the collected words into several groups according to the preceding sonorant consonants, the positions, and the presence (or absence) of the stress, of the syllable where /ju/ occurs. The results are as follows: 1) the deletion of /j/ depends on the sonorant consonant which /ju/ follows, the position where it occurs, and the presence of the stress which /ju/ bears; 2) though the influence of the sonorant consonants is strong, the position and stress also have non-trivial effect on the deletion of /j/, that is, the word-initial syllable and the stressed syllable prefer the deletion of /j/, and word-medial and unstressed syllable usually retain /j/; 3) the stress and position factors play their own roles even in the context where the effect of /n, l, r/ is dominant.

• 대한조선학회논문집
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• 제54권6호
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• pp.476-484
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• 2017

The purpose of this paper is to verify the structural integrity of a region with numerous penetration-holes in offshore structures such as semi-submersible rig and FPSO. In order to effectively check the yielding and buckling strength of plate members with penetration-holes, a screening analysis program was developed with the FE analysis tool to generate fine meshed model using the theoretical and analysis methods. When a hole is appeared in the plate structure members, the flow of stress is altered such that concentrations of stress form near the hole. Stress concentrations are of concern during both preliminary and detail design and need to be addressed from the perspectives of strength. To configure the geometrical shape, very fine meshed FE analysis is needed as the most accurate method. However, this method is practically impossible to apply for the strength verifications for all perforated plates. In this paper, screening analysis method was introduced to reduce analysis tasks prior to detailed FE analysis. This method is applied to not only the peak stress calculation combined stress concentration factor with nominal stress but also nominal equivalent stress calculation considering cutout effects. The areas investigated by very fine meshed analysis were to be chosen through screening analysis without any reinforcements for penetration-holes. If screening analysis results did not satisfy the acceptance criteria, direct FE analysis method as the 2nd step approach were applied with one of the coarse meshed model considering hole or with the very fine meshed model considering the hole shape and size. In order to effectively perform the local fine meshed analysis, automatic model generating program was developed based on the MSC/PATRAN which is pre-post FE analysis program. Buckling strength was also evaluated by Common Structure Rule (CSR) adopted by IACS as the stress obtained from very fine meshed FE analysis. Due to development of the screening analysis program and automatic FE modeling program, it was able to reduce the design periods and structural analysis costs.