• 한국약용작물학회지
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• 제8권2호
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• pp.109-116
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• 2000

115 종의 생약재 중에서 식품부패성 및 병원성 미생물을 대상으로 항균활성을 나타내는 생약자원을 검색한 결과 식품부패성 세균 Bacillus subtilis에 강한 항균활성을 보인 것은 단삼, 관중, 가자, 독활, 파고지, 오미자이었으며, 식중독균인 효모 Candida albicans IFO6258에 대해서는 지모와 관중 두 생약재가, 식품부패성 효모 Schizosaccharomyces sp.에 대해서는 지모, 관중, 원지가 각각 강한 항균 활성을 보였다. Streptococcus mutans에 대해서는 관중, 단삼, 소목, 방기, 오미자, 오배자, 고삼, 목향이 강한 항균 활성을 보였다. 피부 각층 및 모발에 침입하여 피부병을 야기하는 백선균 Trichonphyton mentagrophytes에 대해서는 지모, 강황, 목향, 호장근, 지유, 황금, 가자들이 항균활성을 나타내었다 . 항균spectrum이 넓으면서 비교적 항균활성이 강한 관중, 단삼, 가자, 황금에 항균활성이 강한 생약자원인 가자, 계피, 방기, 황금등의 생약재를 각각 1 : 1로 혼합한 추출물에 대한 항균활성을 조사한 결과 모든 혼합 추출물에서 단독 추출물 보다 강한 항균활성을 나타내었다.

• 한국미생물·생명공학회지
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• 제46권2호
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• pp.127-134
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• 2018

본 연구에서는 해조류인 Umbraulva japonica의 표면에서 79개의 세균을 분리하였다. 16s rRNA 유전자 분석 결과, 주요 계통군은 Proteobacteria (74.69%), Actinobacteria (2.53%), Fimicute (2.53%), Bacteroidetes (20.25%)로 4개의 문(Phylum)이 관찰되었고, 7개의 강(Class), 13개의 목(Order), 17개의 과(Family), 31개의 속(Genus)을 확인하였다. 계통학적 분석 결과 3개의 균주가 표준균주와 97% 이하의 유사성을 보여 신속 또는 신종으로 보고될 가능성이 있다고 여겨지며, 향후 표준균주들과 함께 추가적인 신종 실험이 수행되어야 할 것으로 사료된다. 분리된 79 균주를 이용하여 인체 및 어류 병원균을 대상으로 항균 활성을 확인하였다. UJT7, UJT20, UJR17의 균체 현탁액이 Vibrio vulnificus에 대하여 항균 활성을 나타냈으며 UJR17의 균체 현탁액은 V. vulnificus와 Streptococcus parauberis에 항균 활성능이 있음을 확인하였다. UJT7은 Bacillus sp., UJT20과 UJR17은 Pseudomonas sp.로 확인되었으며 다양한 활용을 위한 추가적인 실험을 수행한 후 유익하게 이용 될 수 있을 것이라 사료된다.

• 한국치위생학회지
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• 제18권5호
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• pp.831-842
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• 2018

Objectives: This study was conducted to reveal the biological basis of dental plaque and preschool children's dental health status through repetitive and continuous toothbrushing instruction to preschool children, using quantitative criteria, and to emphasize the importance of the role of an oral health manager for the prevention of dental caries. Methods: After IRB approval, toothbrushing instruction and oral microbial specimen collection were conducted with children of preschool age at the Daycare Center attached to D. University, Busan. Specimens from 27 children were characterized by analysis with real-time multi-chain enzymatic polymerization reaction technique at an agency specializing in genetic analysis. Results: In a survey of the children's dental health behaviors, 48.1% responded that the toothbrushing time is 91 to 150 seconds; 66.7% responded that the frequency of toothbrushing is more than three times per day; 81.5% stated that they performed tongue brushing; and 81.5% brushed their teeth. Regarding levels of dental cariogenic bacteria, after children received continuous toothbrushing instruction, there were reductions in both Streptococcus mutans and Streptococcus sobrinus. Regarding toothbrushing time, the time increased in relation to the presence of instruction. Regarding Quigley Hein Index, the index decreased in the early and middle periods upon addition of instruction, then increased in the middle and later periods. Conclusions: Through persistent and repetitive toothbrushing instruction, healthy behavior can be positively established in children of preschool age; it is necessary to prepare institutional measures so opportunities for dental health education can be consistently provided and maintained. In the future, it will be necessary to conduct a follow-up study to verify the factors affecting toothbrushing time and volume of oral pathogens.

• 한국식품과학회지
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• 제32권6호
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• pp.1358-1364
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• 2000

생산량이 풍부하고 가공성이 높지 않은 농산물인 무, 감자, 생강, 사과, 대추를 이용한 과채발효액을 제조하기 위하여, 박테리오신 생성 젖산균과 효모를 이용하여 발효를 순행하고, 발효액의 유기산 농도와 향기성분, 박테리오신의 생성 및 항균활성 등을 측정하여 저장성을 지닌 음료로서의 가능성을 검토하였다. 박테리오신 생성 젖산균인 Lc. lactis를 이용한 과채 젖산발효액의 pH는 4.07, 산도는 0.36%이며, lactic acid, citric acid, malic acid, succinic acid 등의 유기산을 함유하고 있으며, 박테리오신의 생성이 확인되었다. 발효중에 생성된 박테리오신의 항균역가는 냉장저장 중에도 매우 안정하였으며, Listeria monocytogenes와 Staphylococcus aureus, 그리고 충치 원인균인 Streptococcus mutans에 대한 저해효과가 있었다. 젖산발효액에 원료 혼합액에서 분리한 효모를 접종하여 후발효시킨 효모발효액에서는 일부 유기산의 소모로 인해 산도가 약간 감소하였으나, succinic acid의 함량은 1.5배정도 높아진 것으로 나타났다. 젖산발효액에서 검출된 ester의 종류는 불쾌하고 쓴맛을 내는 phthalate 종류가 많은 반면, 효모 접종구에서는 과일향을 내는 ester 종류가 많이 검출되었고, alcohol종류도 증가한 것으로 나타나 젖산발효액의 풍미개선에 기여한 것으로 판단된다.

• 한국약용작물학회지
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• 제17권5호
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• pp.335-340
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• 2009

To evaluate the availability of Cudrania tricuspidata Bureau as a natural source of antimicrobials, the antimicrobial activity of methanol extracts of harvested parts was investigated using the paper disc diffusion method. The extracts from leaves and root bark had broad antimicrobial activity against various bacteria, including Bacillus subtilis, Bacillus cereus, Staphylococcus aureus, Streptococcus mutans, Listeria monocytogenes, Enterobacter aerogenes, Enterococcus faecalis, Salmonella choleraesuis subsp. choleraesuis, Vibrio vulnificus, and Pseudomonas aeruginosa and inhibited Bacillus cereus, Staphylococcus aureus, and Listeria monocytogenes, agents of food poisoning especially well. The extract from ripe fruit had a very high antimicrobial activity against Staphylococcus aureus with a 20.2 mm of clear zone at 50 mg/mL sample concentration. These results indicated that Cudrania tricuspidata could be used as new source for developing natural antimicrobial agents.

• 한국염색가공학회지
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• 제29권4호
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• pp.247-255
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• 2017

Scutellaria Baicalensis(SB) is widely used in traditional and modern oriental medicine. It possesses several biology activities such as anti-oxidative, anti-inflammatory, antimicrobial and antiviral activities. In this study, a functional high density polyethylene (HDPE) fabric with antimicrobial properties was developed using zeolite microparticles as a SB extract delivery carrier. Zeolites loaded with SB extract were prepared by immersing in an SB extract aqueous solution. The average size of the SB extract-loaded zeolites was about 0.1 to $2.0{\mu}m$, and the morphology of the zeolites was not altered after SB extract binding. The resulting SB extract-loaded zeolites were then immobilized homogeneously onto the HDPE fabric using acrylic binder. The encapsulation efficiency of SB extract to the zeolite was more than 45%. The in vitro release test of SB extract-loaded zeolites containing HDPE fabrics showed release of 35% of the total SB extract by day 1 in a 24hours immersion study. Moreover, the SB extract-loaded zeolites containing HDPE fabrics showed effective antimicrobial activity against Streptococcus mutans, Staphylococcus aureus, and Klebsiella pneumoniae, indicating that this innovative delivery platform potently imparted antimicrobial activity to the HDPE fabric. In conclusion, the current study suggests that the HDPE fabric containing the SB extract-loaded zeolites microparticle carrier system has potential as an effective antimicrobial textile such as safety gloves, protective gloves etc.

• 한국콘텐츠학회논문지
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• 제18권2호
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• pp.361-369
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• 2018

본 연구는 흡연자의 구강 내 환경을 관찰하여 흡연자의 구강관리용품 사용에 대한 중요성과 구강 환경 개선을 위해 지속적이고 체계적인 구강관리용품 사용의 필요성을 확인하기 위해 강원도 지역의 대학생들을 대상으로 흡연자군 12명, 비흡연자군 12명에게 설문조사 및 실험조사를 실시하였다. 조사결과 두 집단 모두 구강관리용품 사용율은 75%로 나타났으며, 그 중 치실 70.7%으로 구강관리용품 중 치실을 가장 많이 사용하는 것으로 나타났다. 비흡연자군보다 흡연자군이 구강관리용품 사용 후 PHP Index, Streptococcus.mutans 수, snyder 활성도가 감소한 것으로 나타났고, 비흡연자군보다 흡연자군이 구강관리용품 사용 후 pH가 증가한 것으로 나타났다. 본 연구조사 결과 구강관리용품의 사용이 전반적으로 흡연자의 구강건강을 증진시키고 구강 환경을 개선할 수 있다고 나타났다. 구강관리용품에 대한 교육을 실시하면 흡연자들의 동기부여에 도움이 될 것으로 사료된다.

• 치위생과학회지
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• 제18권1호
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• pp.18-23
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• 2018

Propolis has been used as a natural remedy in folk medicine worldwide. The antibacterial, antiviral, antifungal, and antiprotozoal aspects of its antimicrobial properties have been widely investigated. However, few studies focused on its applications in dentistry. Many dental diseases are related to various microorganisms in the oral cavity. In this study, we assessed the antimicrobial activity of Korean propolis extract, collected from 6 different regions, on oral pathogenic microorganisms. The propolis samples, collected from 6 different regions (P1: Uijeongbu, P2: Ansan, P3: Hongcheon, P4: Iksan, P5: Gwangju, and P6: Sangju), were dissolved in ethanol at two different concentrations (10 and 50 mg/ml). Three oral bacteria (Streptococcus mutans, Staphylococcus aureus, and Enterococcus faecalis) and one fungus (Candida albicans) were activated in general broth for 24 hours. Microorganisms were diluted and spread onto agar plates, onto which sterilized 6 mm filter papers with or without each propolis sample were placed. After 24 hours of incubation, clear zones of inhibition were observed. All tests were performed in triplicate. The propolis samples showed significant antibacterial and antifungal activity on oral pathogenic microorganisms; in addition, low-concentration groups showed outstanding antimicrobial efficacy on the 4 different microorganisms. Among the samples, P6 had significantly higher antibacterial activity than that of the others against three different bacteria. In particular, a high concentration of P6 showed a significant antifungal effect. In conclusion, we confirmed that Korean propolis has an inhibitory effect on oral pathogenic bacteria and fungi. Therefore, we suggest the possibility of developing oral medicine and oral care products based on Korean propolis.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• 제33권6호
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• pp.601-608
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• 2007

The aim of this study was to evaluate the effect of mouthrinse products containing deep sea water. We used original deep sea water(DSW) and processed deep sea water desalinated by reverse osmosis at one time(DDW-1), by reverse osmosis at two times(DDW-2) and concentrated by reverse osmosis(CDW). We made 2 kinds of mouthrinse products containing CDW and other agents for smell and taste and one product without deep sea water. The negative control was distilled water. In vivo study, the dental plaque index scores and the gingival index scores were reduced after 4 weeks mouthrinsing three times daily with 4 kinds of deep sea water and 3 kinds of mouthrinse products(p<0.05). The pH of dental plaque in 1 minute after mouthrinsing was not higher than 5.5 in all solutions, but the pH in 20 minutes after mouthrinsing was higher than 5.7 in DSW, CDW and 3 kinds of products which had higher mineral contents. In vitro study, the mouthrinse solutions containing the higher mineral contents were also the more effective in reduction of methyl mercaptan which is one of the causes of halitosis. The 2 kinds of products containing deep sea water killed Streptococcus mutans(ATCC 25175) in culture plates in one minute. These results indicate the usability of deep sea water in mouthrinses for oral hygiene management.

• 대한한방소아과학회지
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• 제10권1호
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• pp.323-349
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• 1996

Gamitonggyutang has been used for treatment of rhinitis in oriental medical science. It is reported that Gamitonggyutang has a good effect on sinusitis in clinical medicine. So this study analysed the effect of Gamitonggyutang on anti-inflammatory, analgenic, anti-Allergic effect and Antibacterial Activity. The result were summerised as follows; 1. Gamitonggyutang extract decreased the edema indused by Carregennin at 200mg/kg and 400mg/kg. 2. Gamitonggyutang extract decreased the protein exudation indused by CMC-pouch at 200mg/kg and 400mg/kg. 3. Gamitonggyutang extract showed the ataralgesia at 800mg/kg by measurement of writhing syndrome, paw licking time and escape time induced by the i.p. infection of acetic acid and hot plate. 4. Gamitonggyutang extract decreased the effluent of vascular permeability indused by Evans blue at 600mg/kg and 800mg/kg. 5. Gamitonggyutang extract decreased the acute edema indused by Carregennin about 2 and 4 hours but didn't show useful effect. 6. Gamitonggyutang extract decreased the death rate, resulted from the effect of active systemic anaphylaxis reaction indused by CGG, but didn't show useful effect.. 7. Gamitonggyutang extract showed the growth inhibitory effect of each bacterias at $52mg/m{\ell}$. 8. Gamitonggyutang extract suppressed the growth of Streptococcus mutans 10449, and showed the supression of acid fabrication in case of 1:10 more than 1:100.