• The Plant Pathology Journal
• /
• v.23 no.4
• /
• pp.300-307
• /
• 2007

Previously, a chloroplast-localized Cu,Zn superoxide dismutase (chCu,ZnSOD) was isolated from lily and the sense- and antisense- sequences of the lily chCu,ZnSOD were used to transform potato plants. Two selected lines, the sense- and anti-sense strand of transgenic plants, were further characterized for resistance to Erwinia carotovora, which is a severe pathogen affecting potato plants. Only the sense-strand transgenic potato, which contained less $O_2^{.-}$ and more $H_2O_2$ than wild-type and antisense-strand transgenic plants, showed increased resistance to E. carotovora. Additional studies using $O_2^{.-}$ or $H_2O_2$ scavengers in wild-type, sense-strand, and antisense-strand transgenic plants suggest that resistance to E. carotovora is induced by reduced $O_2^{.-}$ and is not influenced by $H_2O_2$. To the best of our knowledge, this report is the first study suggesting that resistance to E. carotovora is enhanced by reduced $O_2^{.-}$, and not by increased amounts of $H_2O_2$.

• Journal of Applied Biological Chemistry
• /
• v.43 no.3
• /
• pp.156-160
• /
• 2000

The possible mechanism of the DNA strand breaking activity of the hot-water extract of Fructus Chaenomelis (dried fruit of Chaenomeles sinensis) in a closed circular duplex replica form DNA (RFI DNA) was studied through agarose gel electrophresis under various conditions. Induction of DNA strand scission by the hot-water extract of C. sinensis occurred in dose and time-dependent manners. $Cu^{2+}$ was indispensable for the induction of DNA strand breakage. Exogeneous chelating agents inhibited the DNA breaking activity, conforming the catalytic action of $Cu^{2+}$ on generation of free radicals responsible for oxidative damage. Antioxidant enzymes and some radical scavengers were used to investigate the major radical species triggering the DNA strand scission, demonstrating that a highest inhibitory activity was found in the presence of catalase, while less in the presence of tiron (a scavenger for superoxide radical), 2-aminoethyl-isothiuroniumbromide-HBr, cysteamine (scavengers for hydroxyl radical), and 1,4-diazabicyclo [2,2,2] octane (a scavenger for singlet oxygen) in decreasing order. The findings implied that oxygen radical species generated in presence of transition divalent cation during the oxidation of some compounds contained in the hot-water extract of C. sinensis is mainly responsible for inducing genotoxicity.

• Proceedings of the Korean Society of Toxicology Conference
• /
• 2003.10b
• /
• pp.160-160
• /
• 2003

Antioxidative potentials of estrogen and genistein were compared by measuring the degree of protection against plasmid DNA strand breakage induced by peroxyl free radicals using the DNA strand scission assay with pBR322 DNA. Genistein decreased DNA strand breakage by AAPH radical treatment at the all of three concentrations tested (0.5, 1.0, 1.5 $\mu\textrm{g}$/$m\ell$) with the range of 89.5% to 99.6%.(omitted)

• Proceedings of the Computational Structural Engineering Institute Conference
• /
• 2008.04a
• /
• pp.133-138
• /
• 2008

This study proposes a novel method for in-service evaluation of force in an external prestressing 7-wire tendon which is employed for retrofitting bridge superstructure. For this propose, a smart strand 7.0m long whose king wire is replaced by a steel tube and the FBG sensor, is developed. Performance of the strand is demonstrated through loading-unloading tests for a RC T-shaped beam 6.4m long. Finally, a couple of test results are presented to discuss effect of temperature change in the FBG sensor.

• Transactions of the Korean Society of Mechanical Engineers A
• /
• v.25 no.1
• /
• pp.127-134
• /
• 2001

Wire ropes are widely used in cable car, suspension bridge and elevator, etc. and composed of single or multi-layer strands. It is difficult to find out the characteristics of a strand or wire rope because of complicated geometry and contact condition. In this study, the axial stiffness is evaluated using finite element method and reliable finite element analysis model is presented, taking into consideration the convergence on the length. The axial stiffness predictive equation of a strand is developed using analysis of variance, which can be applicable for characterizing the relationship between load and displacement when the strand configuration is determined.

• Korean Journal of Fisheries and Aquatic Sciences
• /
• v.36 no.4
• /
• pp.346-351
• /
• 2003

Single-cell gel electrophoresis (comet assay) was used to detect DNA single strand break in blood cells from several marine fish species. Three fish species were collected from Georgia coastal area. Mummichog, Fundulus heteroclitus showed higher DNA damage than sea bass, Lateolabrax japonicus and trout, Oncorhynchus masou masou under the same experimental conditions. Mummichogs had more alkaline-labile sites on their DNA than other fish species. The comet assay with mummichog blood cells at pH 12.5 showed a dose-response curve with the increasing concentrations of hydrogen peroxide. While the isolated leucocytes showed no increase of DNA damage after in vitro exposure to 2-methyl-1,4-naphthoquinone (MNQ), erythrocytes showed dose-dependent DNA damage. These results indicate that the comet assay can be applied successfully as a bioassay using erythrocyte for environmental monitoring.

• Journal of Microbiology and Biotechnology
• /
• v.16 no.12
• /
• pp.2004-2007
• /
• 2006

An efficient method for the in vitro reassembly of homologous DNA sequences is presented. The proposed method involves obtaining single strands of homologous genes and hybridizing them to obtain partially hybridized heteroduplex DNA; cleaving the single-stranded regions of the heteroduplex DNA using S1 nuclease to generate double-strand DNA fragments; denaturing the double-strand DNA fragments to generate single-strand DNA fragments; conducting a series of polymerase chain reactions (PCR) using the single-strand DNA fragments as internal primers and a mixture of homologous DNA as templates to obtain elongated reassembled DNA; and finally, amplifying the reassembled DNA by a PCR using terminal primers. As a result, DNA reassembly could be achieved between homologous genes with a sequence similarity as low as 78%.

• Proceedings of the Korea Institute of Applied Superconductivity and Cryogenics Conference
• /
• 1999.02a
• /
• pp.39-42
• /
• 1999

The characteristics of Nb3Sn strands shich will be used for the been investigated. The physics requirements for the KSTAR operation demands the two conditions, high non-Cu critical current density and low contradictory to each other. It is recent that superconductor manufacturers. IGC and Mitsubishi, have succeded in the production of the strand which satisfies the ITER HP-1 and HP-2 conditions at the same time. It has been determined that this type of strand, so-called HP-3, will be used for the KSTAR magnets and its properties, critical current density and hysteresis loss investigated by the several institutes are presented here. On the basis of the round robin tests for many strand samples under various optimized and the vendor will be seleted.

• Proceedings of the Korea Concrete Institute Conference
• /
• 2009.05a
• /
• pp.93-94
• /
• 2009

In this paper, the bond performance between prestressing strand and Ultra High Performance Concrete members are verified to collect the basis data of UHPC increased in practical uses recently. Concrete pouring direction, strand location and strand size were the main parameters of UHPC. As a result, the initial bond performances are influenced by all above parameters.

• Proceedings of the Korean Society of Propulsion Engineers Conference
• /
• 1994.11a
• /
• pp.39-45
• /
• 1994

Combustion characteristics of the solid composite propellants were studied from burning rate, ignition and steady combustion processes, and structure of the extinguished surfaces. Optical Vacuum Strand Burner (OVSB) system was desisted and configured to study those. Burning rates of the propellants were measured by OVSB at low pressure range by developed ten method. video camera(30 frames/s) was used to take potographs of the phenomena of ignition and combustion of propellant within the test cell of the OVSB. Burning surfaces of the propellants that were extinguished by rapid depressurization method were analyzed with Scanning Electron Microscope. (SEM).