• Proceedings of the Korea Concrete Institute Conference
• /
• 2009.05a
• /
• pp.147-148
• /
• 2009

The anchorage system unified both PS strand and bar effectively introduced a prestress into a flexural concrete members. This study examined the relation between the anchorages of both ends and the introduced force and derived the equation for design.

• Proceedings of the Korean Society of Precision Engineering Conference
• /
• 2000.05a
• /
• pp.808-811
• /
• 2000

The product of stereolithography is consist of gathering the single strand. Therefore the accuracy of the shape is related to the linear shrinkage of the single strand. The resin temperature change affect on curing properties. This article will propose the interaction between material temperature and shape accuracy by resin temperature control. The main concern of this article is related to the improvement of end product's shape accuracy by the persuit for the filles curing criterion.

• Proceedings of the Korea Institute of Applied Superconductivity and Cryogenics Conference
• /
• 2003.10a
• /
• pp.45-48
• /
• 2003

Ramp-rate limitation is a serious obstacle for successful operation of fast charging and discharging magnet Ramp-rate limitation is hard to expect or fully investigate due to its electric and thermo-hydraulics couplings. In this paper, the simplest case of ramp-rate limitation is investigated with two-strand superconducting cable model considering transient heat transfer The simulation results are compared with the experimental results.

• Journal of the Korean Chemical Society
• /
• v.49 no.6
• /
• pp.537-545
• /
• 2005

• Proceedings of the PSK Conference
• /
• 2003.04a
• /
• pp.268.1-268.1
• /
• 2003

Brazilin is the phenolic compound isolated from the Caesalpinia sappan. This compound has shown a wide range of physiological properties, such as hypoglycemic, anticonvulsant, vasorelaxing, and immunomodulating effects. In this study, we have found that brazilin induced DNA strand scissions in the presence of Cu(II) and this DNA cleavages were mediated by reactive oxygen species. (omitted)

• Environmental Mutagens and Carcinogens
• /
• v.21 no.2
• /
• pp.89-94
• /
• 2001

Recently, single cell gel electrophoresis, also known as comet assay, is widely used for the detection and measurement of DNA strand breaks in vitro and in vivo in many toxicological fields such as radiation exposure, human monitoring and toxicity evaluation. As well defined, comet assay is a sensitive, rapid and visual method for the detection of DNA strand breaks in individual cells. Briefly, a small number of damaged cells suspended in a thin agarose gel on a microscope slide were lysed, unwinded, electrophoresed, and stained with a fluorescent DNA binding dye. The electric current pulled the charged DNA from the nucleus such that relaxed and broken DNA fragments migrated further. The resulting images which were subsequently named for their appearance as comets, were measured to determine the extent of DNA damages. However, some variations could be occurred in procedures, laboratories's conditions and kind of cells used. Hence, to overcome and to harmonize these matters in comet assay, International Workshop on Genotoxicity Test Procedure (IWGTP) was held with several topics including comet assay at Washington D.C. on March, 1999. In spite of some consensus in procedures and conditions in IWGTP, there are some problems still remained to be solved. In this respect, we attempted to set the practical optimal conditions in the experimental procedures such as lysis, unwinding, electrophoresis and neutralization conditions and so on. First of all, we determined optimal lysis and unwinding time by using 150 $\mu$M methyl methanesulfonate (MMS) which is usually used concentration. And then, we determined optimal positive control concentrations of benzo(a)pyrene (BaP) and MMS in the presence and absence of S9 metabolic activation system, respectively.

• Korean Journal of Microbiology
• /
• v.47 no.2
• /
• pp.158-162
• /
• 2011

Amino acid sequence alignment shows that $\underline{P}$roteus $\underline{m}$irabilis $\underline{t}$ranscription $\underline{r}$egulator (PMTR) has cystein sequence homology at metal binding domain to CueR (copper resistance) protein, which conserves two cysteins (Cys 112 and Cys 120 in PMTR). Gel shift assay revealed that PMTR protein bound to promoter region of Escherichia coli copA (copper-translocating P-type ATPase) and Proteus mirabilis atpase (putative copper-translocating P-type ATPase) genes except that of E. coli zntA (zinc-translocating P-type ATPase) gene. DNase I protection experiment indicated that PMTR protein protected the region over -35 box and close to -10 box. DNase I hypersensitive bases were shown at C and A bases of labeled template strand and at G and C bases of labeled non-template strand of DNA. These hypersensitive bases were appeared in other metalloregulatory proteins of MerR family, which suggests protein-induced DNA bending.

• Structural Engineering and Mechanics
• /
• v.47 no.3
• /
• pp.383-399
• /
• 2013

High-strength strands are widely used as a key structural element in cable-stayed bridges and prestressed concrete structures. Conventional strands for stay cable and tendons in prestressed concrete structures are ${\phi}$15.7mm coated seven-wire strands and ${\phi}15.2mm$ uncoated seven-wire strands, respectively, but the ultimate strengths of both strands are 1860MPa. The objective of this paper is to investigate the tensile behavior of a newly developed ${\phi}15.7mm$ 2,200 MPa coated strand and a ${\phi}15.2mm$ 2,400 MPa uncoated strand according to various types of mono anchorages and to propose appropriate anchorages for both strands. Finite element analyses were initially performed to find how the geometry of the anchor head affects the interaction among the anchor head, the wedge and the strand and to find how it affects the stress distributions in both parts. Tensile tests for the new strands were carried out with seven different types of mono anchorages. The test results were compared to each other and to the results obtained from the tensile tests with a grip condition. From the analysis and the test results, desirable mono anchorages for the new strands are suggested.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
• /
• v.35 no.1
• /
• pp.1-6
• /
• 2009

DNA double-strand breaks (DSBs) occur commonly in the all living and in cycling cells. They constitute one of the most severe form of DNA damage, because they affect both strand of DNA. DSBs result in cell death or a genetic alterations including deletion, loss of heterozygosity, translocation, and chromosome loss. DSBs arise from endogenous sources like metabolic products and reactive oxygen, and also exogenous factors like ionizing radiation. Defective DNA DSBs can lead to toxicity and large scale sequence rearrangement that can cause cancer and promote premature aging. There are two major pathways for their repair: homologous recombination(HR) and non-homologous end-joining(NHEJ). The HR pathway is a known "error-free" repair mechanism, in which a homologous sister chromatid serves as a template. NHEJ, on the other hand, is a "error-prone" pathway, in which the two termini of the broken DNA molecule are used to form compatible ends that are directly ligated. This review aims to provide a fundamental understanding of how HR and NHEJ pathways operate, cause genome instability, and what kind of genes during the pathways are associated with head and neck cancer.

• Journal of the Korea Concrete Institute
• /
• v.27 no.1
• /
• pp.11-20
• /
• 2015

An unbonded post-tensioned anchor using a 15.2 mm diameter 7-wire strand was developed based on finite element analysis and experimental testing. In order to evaluate its performance, static load tests and load transfer tests were conducted following KCI-PS101. The static load tests and additional strand tensile tests confirmed that the developed anchor had a capacity more than nominal tensile strength of a 7-wire strand without any damage or deterioration. According to the result of load transfer tests for many different reinforcing details, specimens with no additional reinforcing bars sustained at least 1.64 times the nominal tensile strength of the strand.