• Proceedings of the Korean Society for Technology of Plasticity Conference
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• 2004.08a
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• pp.307-316
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• 2004

This study examined the cause of the wrinkle defect which is frequently encountered in wire rod rolling of low carbon steel$(C0.08\~0.13wt.\%)$. Even a small defect on the surface of rolled bars can easily develop into fatal cracks during cold heading process of low carbon steel, and it is therefore necessary to minimize inherent defects on the surface of hot rolled bars. Hot rolling process of low carbon steel was analyzed to identify the cause of the wrinkle defect in conjunction with FE analysis. The integrated analysis revealed that the wrinkle defect initiated in the first stage of rolling, and it was at the billet edge where severe deformation and drastic temperature drop were present. To elucidate the micro-mechanical mechanism of the wrinkle defect, hot compression tests were carried out at various temperatures and strain rates using Gleeble-3800. The surface profile of the each other compressed specimens was compared, and rough surface lines were observed at relatively low temperatures. Those surface defects can develop into wrinkles during multi-pass rolling. To control the wrinkle defect in rolling, it is necessary to design an adequate caliber which can minimize the loss of ductility, and thereby prevent flow localization. To use the result of this study fur other steels, the quantitative measure of the wrinkle defect and flow localization parameter should be proposed.

• Journal of Marine Bioscience and Biotechnology
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• v.7 no.1
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• pp.11-18
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• 2015

An extracellular alkaline ${\alpha}$-D-mannosidase produced by a strain named as MA-01 was produced and its preliminary enzyme activity was characterized. Upon determining the 16S rDNA sequence and its homology search, the strain was identified to be one of species of the Bacillus safensis. Localization of enzyme was elucidated that ${\alpha}$-D-mannosidase can be found in culture medium as an extracellular enzyme. In addition, partial enzyme activity of 63% compared with the extracellular enzyme activity was observed in membrane protein. The optimal pH and temperature of the ${\alpha}$-D-mannosidase were pH 7.5 and $37^{\circ}C$, respectively. The $K_m$ and $V_{max}$ values of the ${\alpha}$-D-mannosidase in crude enzyme toward p-nitrophenyl-${\alpha}$-D-mannopyranoside were determined to be $455.6{\mu}M$ and $10.8{\mu}mole/min/mg$ of protein, respectively. To the best of our knowledge, this is the first report described the alkaline ${\alpha}$-D-mannosidase from the family of B. safensis.

• Proceedings of the Korea Concrete Institute Conference
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• 2002.05a
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• pp.929-934
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• 2002

Eight full-scale columns were constructed and tested under monotonic axial compression loading to investigate the influence of headed bars on the confinement of the concrete. One column represented a column with no transverse reinforcement and another column had poor detailing and little confinement. A third column contained seismic hoops and crossties, which represented current detailing practice for significant confinement. A fourth column test is conducted to investigate the response with the seismic crossties replaced by headed bars. Two column specimens were constructed and tested with all of the transverse reinforcement provided by headed bars. These six specimens enabled an assesment of the effectiveness of headed bars in confining the concrete. It was found that the use of headed bars improved the confinement of the columns. Two additional specimens were constructed without any transverse reinforcement. These columns were later retrofitted, by drilling horizontal holes in the columns, adding special headed bars (one head fixed and the other head threaded) and then filling the drilled holes with epoxy. These retrofitted specimens with these added headed bars provided insight into the rehabilitation of older structures containing poorly detailed columns. All of the test specimens were instrumented to determine strain localization during failure and to monitor the strain in the longitudinal and transverse reinforcement.

• Interaction and multiscale mechanics
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• v.4 no.3
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• pp.187-206
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• 2011

Accurate estimations of pre-failure deformations and post-failure responses of geostructures require that the simulation tool possesses at least three main ingredients: 1) a constitutive model that is able to describe the macroscopic stress-strain-strength behavior of soils subjected to complex stress/strain paths over a wide range of confining pressures and densities, 2) an embedded length scale that accounts for the intricate physical phenomena that occur at the grain size scale in the soil, and 3) a computational platform that allows the analysis to be carried out beyond the development of an initially "contained" failure zone in the soil. In this paper, a two-scale micropolar plasticity model will be used to incorporate all these ingredients. The model is implemented in a finite element platform that is based on the mechanics of micropolar continua. Appropriate finite elements are developed to couple displacement, micro-rotations, and pore-water pressure in form of $u_n-{\phi}_m$ and $u_n-p_m-{\phi}_m$ (n > m) elements for analysis of dry and saturated soils. Performance of the model is assessed in a biaxial compression test on a slightly heterogeneous specimen of sand. The role of micropolar component of the model on capturing the post-failure response of the soil is demonstrated.

• Smart Structures and Systems
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• v.14 no.3
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• pp.285-305
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• 2014

This study employs a novel beam-type wavelet finite element model (WFEM) to fulfill an adaptive-scale damage detection strategy in which structural modeling scales are not only spatially varying but also dynamically changed according to actual needs. Dynamical equations of beam structures are derived in the context of WFEM by using the second-generation cubic Hermite multiwavelets as interpolation functions. Based on the concept of modal strain energy, damage in beam structures can be detected in a progressive manner: the suspected region is first identified using a low-scale structural model and the more accurate location and severity of the damage can be estimated using a multi-scale model with local refinement in the suspected region. Although this strategy can be implemented using traditional finite element methods, the multi-scale and localization properties of the WFEM considerably facilitate the adaptive change of modeling scales in a multi-stage process. The numerical examples in this study clearly demonstrate that the proposed damage detection strategy can progressively and efficiently locate and quantify damage with minimal computation effort and a limited number of sensors.

• Structural Engineering and Mechanics
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• v.54 no.2
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• pp.379-392
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• 2015

A number of acceleration-based damage detection methods have been developed but they have not been widely applied in engineering practices because the acceleration response is insensitive to minor damage of civil structures. In this article, a damage detection approach using the long-gauge strain sensing technology and the principle component analysis technology is proposed. The Long gauge FBG sensor has its special merit for damage detection by measuring the averaged strain over a long-gauge length, and it can be connected each other to make a distributed sensor network for monitoring the large-scale civil infrastructure. A new damage index is defined by performing the principle component analyses of the long-gauge strains measured from the intact and damaged structures respectively. Advantages of the long gauge sensing and the principle component analysis technologies guarantee the effectiveness for structural damage localization. Examples of a simple supported beam and a steel stringer bridge have been investigated to illustrate the successful applications of the proposed method for structural damage detection.

• Journal of the Earthquake Engineering Society of Korea
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• v.7 no.4
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• pp.89-95
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• 2003

In this paper, an algorithm to locate and size damage in a complex truss structure using the time domain response is presented. Sampled response data for specific time interval is spatially expanded over the structure to obtain the mean train energy for each element of the structure. The mean strain energy for each element is, in turn, used to build a damage index that represents the ratio of the stiffness parameter of the pre-damaged to the post-damaged structure. The validity of the methodology is demonstrated using data from a numerical example of a space truss structure with simulated damage. Also in the example, the effects of noisy data on the proposed algorithm are examined by adding random noised to the response data.

• Journal of Veterinary Science
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• v.25 no.4
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• pp.54.1-54.16
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• 2024

Importance: As one of the main etiologic agents of infectious diseases in pigs, pseudorabies virus (PRV) infections have caused enormous economic losses worldwide. EP0, one of the PRV early proteins (EP) plays a vital role in PRV infections, but the mechanisms are unclear. Objective: This study examined the function of EP0 to provide a direction for its in-depth analysis. Methods: In this study, the EP0-deleted PRV mutant was obtained, and Tandem Mass Tag-based proteomic analysis was used to screen the differentially expressed proteins (DEPs) quantitatively in EP0-deleted PRV- or wild-type PRV-infected porcine kidney 15 cells. Results: This study identified 7,391 DEPs, including 120 and 21 up-regulated and down-regulated DEPs, respectively. Western blot analysis confirmed the changes in the expression of the selected proteins, such as speckled protein 100. Comprehensive analysis revealed 141 DEPs involved in various biological processes and molecular functions, such as transcription regulator activity, biological regulation, and localization. Conclusions and Relevance: These results holistically outlined the functions of EP0 during a PRV infection and might provide a direction for more detailed function studies of EP0 and the stimulation of lytic PRV infections.

• Journal of the Korean Geotechnical Society
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• v.24 no.7
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• pp.25-36
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• 2008

The plane strain test can reproduce the real field condition and failure behavior precisely over other laboratory shear tests. Accordingly, this test has been utilized to investigate the shearing behaviors associated with overall failure behavior and local deformation of soils. However, most plane strain tests have been carried out with restrained end plates due to difficulties in manufacturing the equipment and also performing it. This restraint induces different results with real field because of shear stress on end plates. In this study, plane strain tests with/without bottom plate restraint were performed on Jumunjin-sand. The measurement of overall and local deformation was accomplished by digital image correlation technique as well as external LVDT. By applying digital image correlation method using two consecutive images captured through the transparent wall, local deformation behavior of various parts inside the specimen was estimated. And the formation and development of shear band caused by the restrained effect of end plate and the deformation mechanism of sand under plane strain condition were examined.

• Journal of the Korea Concrete Institute
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• v.16 no.4 s.82
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• pp.511-520
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• 2004

The fracture energy evaluated from the previous experimental results can be simulated by using the modified singular fracture process zone (S-FPZ) model. The fracture model has two fracture properties of strain energy release rate for crack extension and crack close stress versus crack width relationship $f_{ccs}$ ( w ) for fracture process zone (FPZ) development. The $f_{ccs}$( w ) relationship is not sensitive to specimen geometry and crack velocity. The fracture energy rate in the FPZ increases linearly with crack extension until the FPZ is fully developed. The fracture criterion of the strain energy release rate depends on specimen geometry and crack velocity as a function of crack extension. The variation of strain energy release rate with crack extension can explain theoretically the micro-cracking, micro-crack localization and full development of the FPZ in concrete.