• Journal of Photoscience
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• v.9 no.2
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• pp.86-89
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• 2002

Blue light activates proton pump, and creates electrical gradient across the plasma membrane and drives $K^{+}$ uptake in stomatal guard cells. In this presentation, we provide evidence for regulatory mechanisms of the pump and the identification of blue light receptor. The pump is shown to be the plasma membrane H$^{+}$- ATPase and is activated through phosphorylation of the C-terminus. Phosphorylation occurred and 14-3-3 protein bound to the phosphorylation site. The binding of 14-3-3 protein was required for the H$^{+}$-ATPase activation. We also found that phot1 phot2 double mutant does not respond to blue light but other mutants respond to blue light by stomatal opening. However, all these mutants are capable of stomatal opening in the presence of fusicoccin, an activator of the H$^{+}$-ATPase. These results suggest that both photl and phot2 act as blue light receptors in guard cells.d cells.

• The Plant Pathology Journal
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• v.28 no.2
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• pp.202-206
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• 2012

Root colonization by the rhizobacterium Pseudomonas chlororaphis O6 in Arabidopsis thaliana Col-0 plants resulted in induced tolerance to drought and salinity caused by halide salt-generated ionic stress but not by osmotic stress caused by sorbitol. Stomatal apertures decreased following root colonization by P. chlororaphis O6 in both wild-type and ABA-insensitive Arabidopsis mutant plants. These results suggest that an ABA-independent stomatal closure mechanism in the guard cells of P. chlororaphis O6-colonized plants could be a key phenotype for induced systemic tolerance to drought and salt stress.

• Korean Journal of Medicinal Crop Science
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• v.10 no.5
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• pp.409-414
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• 2002

This study was carried out in order to investigate the main traits related to photosynthesis by multiple regression analysis. The photosynthetic rate showed more significant increase in shaded leaves of plant than in non-shaded leaves of plant after shading treatment for 44 days. Negative correlation was found between net photosynthetic rate and SLA(specific leaf area). However, the values obtained from the linear equation were different and were highly significance. From the above results, net photosynthetic rate of shading treatment is higher than that of control in the same SLA. We also found that there were correlation between stomatal conductance and SPAD as well as net photosynthetic rate and stomatal conductance. By the multiple regression analysis, SPAD value, SLA, stomatal conductance and leaf water potential showed a higher correlation coefficient of $r=0.848^{**}$. From the results, out of 4 factors(SPAD value, SLA, stomatal conductance, leaf water potential) stomatal conductance was main factor in the view points of partial regressing.

• Journal of Bio-Environment Control
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• v.6 no.4
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• pp.292-298
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• 1997

These studies were conducted to verify the effect of the supplementary lighting by reflective film mulching and its establishment in the north side of greenhouse on the utility of light at tomato by means of investigating changes of leaf temperatures, stomatal features, transpiration rates and photosynthetic rates. Stomatal density of leaves were high in the reflective film mulching but sizes of stomata were not different. As the osmotic potential in rooting zone was low, the stomatal resistance was high, transpiration rate was low, and leaf temperature was increased by 40.62$^{\circ}C$. And also in the block of reflective film mulching photosynthetic rates were decreased hut chlorophyll contents were not different. Especially, there is an effect of controlling greenhouse whiteflies by treatment of reflective film mulching. It is thought that the reason of high quality or increasing yield at several crops by supplementary lighting, such as reflective film mulching, would be caused by influences of absorption and distribution of nutrients through high transpiration rate and photosynthesis which resulted from increase of stomata.

• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.110-110
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• 2017

Stomata are the main gateways for water and air transport between leaves and the environment. Inward-rectifying potassium channels regulate photo-induced stomatal opening. Rice contains three inward rectifying shaker-like potassium channel proteins, OsKAT1, OsKAT2 and OsKAT3. Among these, only OsKAT2 is specifically expressed in guard cells. Here, we investigated the functions of OsKAT2 in stomatal regulation using three dominant negative mutant proteins, OsKAT2(T235R), OsKAT2(T285A) and OsKAT2(T285D), which are altered in amino acids in the channel pore and at a phosphorylation site. Yeast complementation and patch clamp assays showed that all three mutant proteins lost channel activity. However, among plants overexpressing these mutant proteins, only plants overexpressing OsKAT2(T235R) showed significantly less water loss than the control. Moreover, overexpression of this mutant protein led to delayed photo-induced stomatal opening and increased drought tolerance. Our results indicate that OsKAT2 is an inward-rectifying shaker-like potassium channel that mainly functions in stomatal opening. Interestingly, overexpression of OsKAT2(T235R) did not cause serious defects in growth or yield in rice, suggesting that OsKAT2 is a potential target for engineering plants with improved drought tolerance without yield penalty.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.30 no.1
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• pp.33-38
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• 1985

This experiment was carried out to study in stomatal behaviour of wheat (Chokwang) upon the different soil water potentials, lodging and two different nitrogen treatment. Stomatal aperture was measured at flowering stage under diurnal course with two hour intervals, Stomatal apertures were differently changed according to soil water potentials and leaf position on the stem, showing the rapid closure of stomata at low soil water potential and the narrow opening at low leaf position of stem. Wider stomatal opening appeared at the plot of optimal nitrogen application than that of low nitrogen. It was greatly decreased in opening of stomata at plants lodged, appearing that plant investigated of six days after lodging showed less than 50% than that normal plants, which indicated peculiar decrease of low leaf position of stem at afternoon of diurnal course.

• Journal of Korean Society of Forest Science
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• v.75 no.1
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• pp.51-54
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• 1986

The good growth of Populus alba ${\times}$ P. glandulosa is shown at the foothill of the mountain where good soil moisture is available, but its growth is poor at the upper part of the mountain where soil moisture is limitted. Stomatal frequency, stomatal size and transpiration rates that affect soil moisture demand were determined in order to know how this character is inherited from its parents. Number of stomata per $mm^2$, length and width of stomata from the abaxial leaf and transpiration rate ($g/cm^2$) per day in P. alba ${\times}$ P. glandulosa showed intermediate values between those of their parents, while P. alba showed the highest values and P. davidiana the lowest values. The values of stomatal frequency of P. alba ${\times}$ P. glandulosa were close to the expected mean values of parents in the $x^2$ test, and in the regression analysis the transpiration rates for the hybrid and its midparent had strong relationship with 0.97 of regression coefficient. The fact that these intermediate characteristics were observed in the hybrid suggests incomplete dominance in the hybrid. It is concluded that soil moisture demand in P. alba ${\times}$ P. glandulosa and its parents is closed related to stomatal frequency, stomatal size and transpiration rate.

• Journal of Korean Society of Forest Science
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• v.105 no.3
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• pp.303-308
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• 2016

To investigate the effect of calcium chloride ($CaCl_2$) using for deicing salts in winter on gas exchange and stomatal responses of 3-year-old Prunus serrulata, we treated twice (1 L) $CaCl_2$ solution (0.5%, 1.0% and 3.0%) in the root zone before leaf unfolding. Stomatal conductance ($g_s$), photosynthetic rate ($P_n$), transpiration rate ($T_r$) and water use efficiency (WUE) in the leaves of P. serrulata were decreased with increasing of $CaCl_2$ concentration. Even though stomatal conductance and photosynthetic rate were reduced by $CaCl_2$, intercellular $CO_2$ concentration ($C_i$) in $CaCl_2$ treatments has similar or higher values compared with control. These results suggest that non-stomatal limitation as well as stomatal limitation induced the reduction of photosynthetic rate together. On the other hands, treatment of $CaCl_2$ before leaf unfolding also affected leaf morphology traits. We proposed that reductions of stomatal length and leaf size and high pore density with increasing salinity is adaptative mechanism to reduce the water loss in plant.

• Korean Journal of Medicinal Crop Science
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• v.4 no.4
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• pp.288-293
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• 1996

This study was investigated to obtain basic information for the development of irrigation plans in upland cultivation of Wasabi. Changes of stomatal conductivity and photosynthetic rate of Wasabi, and of the soil water potential during withholding watering were analysed. The stomatal conductivity of Wasabi at $1000{\mu}Em^{-2}s^{-1}$ light intensity was $70mmolem^{-2}s^{-1}$, which was about 49% lower than that of Chinese cabbage, $138mmolem^{-2}s^{-1}$. The temporal changes of light intensity during the daytime did not influence the stomatal conductivity. The soil water potential that decreased stomatal conductivity in Wasabi was about - 50kPa at 10 AM, and about - 30kPa at 3 PM. The photosynthetic rate of Wasabi at$1000{\mu}Em^{-2}s^{-1}$ light intensity was $7.6mgdm^{-2}hr^{-1}$, which was about 50% lower than that of Chinese cabbage, $15.3mgdm^{-2}hr^{-1}$. The duration required for a stable photosynthetic rate was longer in Wasabi than in Chinese cabbage. The soil water potential that decreased photosynthetic rate in Wasabi was about - 50kPa at 10 AM, and about - 30kPa at 3 PM. The stomatal conductivity and photosynthetic rate showed significant positive correlation at various soil water potential. The results indicated that irrigation in wasabi could be done during the daytime when the soil water potential is above - 30kPa, which does not decrease stomatal conductivity and photosynthesis in Wasabi.

• Journal of Korean Society of Forest Science
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• v.66 no.1
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• pp.82-94
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• 1984

The variation of stomatal density and stomatal length of four species of oaks was studied for the purpose of examining the differences among populations and among individual trees within population. Nine populations of Quercus mongolica, four populations of Q. serrata and Q. variabilis respectively, and three populations of Q. acutissima were selected in the natural stands of oaks distributed through the whole country. Twelve leaves were sampled from each of 20 trees from each population. The length of 20 stomata and ten replications of stomatal density were measured from collodion replicas of each leaf under a microscope. Average stomatal densities and lengths ranged through $600-1000/mm^2$ and $19-26{\mu}m$ respectively in all of the species studied. The stomatal densities and lengths presented significant differences statistically at the level of 1 or 5% among populations and among individual trees within population in all the species. Quercus mongolica, especially, showed large variation among populations, while Q. variabilis did very narrow variation compared to the other species. The coefficients of variation of stomatal densities and lengths among individual trees within population exhibited small values of 3.7-12.0% and 1.4-5.3% respectively in all the populations of the species. The average stomatal densities of Q. mongolica showed statistically significant correlation of multiple correlation coefficient of $R_{df{\cdot}2.6}=0.868^*$ and multiple regression equation of $Y=0.041X_1(G.M.T.S.)+0.489X_2(G.M.H.S.)+22.37$ with the sum of growing season mean daily temperature and the sum of growing season mean daily humidity of the stand studied. However the average stomatal lengths showed no relation with the same meteological variables. The figures of frequency distribution of the measurements of leaves or the mean values of individual trees did not show normal distribution curves in some populations. The curves, as well as the results of ANOVA, exhibited the differences among populations.