• Journal of Environmental Science International
• /
• v.25 no.9
• /
• pp.1289-1297
• /
• 2016

The walls of guard cells have many specialized features. Guard cells are present in the leaves of bryophytes, ferns, and almost all vascular plants. However, they exhibit considerable morphological diversities. There are two types of guard cells: the first type is found in a few monocots, such as palms and corn, and the other is found in most dicots, many monocots, mosses, ferns, and gymnosperms. In corns, guard cells have a characteristic dumbbell shape with bulbous ends. Most dicot and monocot species have kidney-shaped guard cells that have an elliptical contour with a pore at its center. Although subsidiary cells are common in species with kidney-shaped stomata, they are almost always absent in most of the other plants. In this study, there were many different stomatal features that were associated with kidney-shaped guard cells, but not dumbbell shaped guard cells, which are present in most grasses, such as cereals. Each plant investigated exhibited different characteristic features and most of these plants had kidney-shaped guard cells. However, the guard cells of Chamaesyce supina Mold, were often more rectangular than kidney-shaped. In contrast, Sedum sarmentosum guard cells were of the sink ensiform type and in Trifolium repens, the guard cells exhibited a more rhombic shape. Therefore, kidney-shaped guard cells could be divided into a number of subtypes that need to be investigated further.

• Korean Journal of Plant Tissue Culture
• /
• v.24 no.6
• /
• pp.323-327
• /
• 1997

Addition of growth inhibitors such as ancymidol, ABA, chloromequat, and pachlobutrazol into MS medium had no effect to preventing vitrification in cultures of Rehmania glutinosa. Anatomical investigation revealed that vitrified thick leaf tissue in vitro had larger intercellular space with poor development of sponge and pallisade tissue compared to those of in vitro grown glaucous and field grown plants. In vitro grown glaucous leaf had smaller and round type stomata showing distinguishable guard and subsidiary cell than those of reestablished plantlets into soil whereas abnormal stomata and poor development of epicuticular wax on the surface of leaf was observed in verified plantlet.

• Journal of Wetlands Research
• /
• v.4 no.2
• /
• pp.1-11
• /
• 2002

The morphology, seed germination, and life history of Salix nipponica were investigated in the wetland of the Nam-River, Jinju, Gyeongsangnam-do, Korea from 2000 to 2001 to provide the basic data necessary for riverine ecosystem restoration through understanding the growth characteristics of Salix nipponica. Salix nipponica had stomata on only lower side and stomata type was paracytic. Salix nipponica produced many small and light seeds. The seed number per mature ament was 1599.4, seed mass of 0.04 mg, and fertilization rate of 66.1%. Seed germination was little affected by light. Germination rate was high and mean germination time was short Under flooding condition, seeds were germinated normally and were viable after as much as 14 days of flooding. But there were no differences under various water depths on germination rates. Tree age was closely correlated with more stem diameter than tree height and there was no difference of growth rate between male and female tree. Growth rate was most rapid for 2 to 3 years after germination, and length growth was almost stopped for more than 11 years even though mass growth was done. Besides even a second-year indivisual was flowered.

• Journal of Forest and Environmental Science
• /
• v.35 no.1
• /
• pp.54-60
• /
• 2019

Plant tissue culture techniques offer quick methods of regeneration of plants of medicinal importance but the survival chances of such plants are always questionable when shifted to the in vivo conditions. The present study enumerates the micromorphological developments in the leaves of in vitro regenerated and field transferred plantlets of Oldenlandia umbellata. The leaves developed in vitro after $4^{th}$ subcultures of multiplication phase and after 6 weeks of field transferred plants were used. Statistically significant differences in the number of stomata, veins, raphides, crystals and trichome density per square mm were observed. The improvements in stomatal apparatus and density (decreased from 41.85 to 32.20), developments in leaf architectural parameters and emergence of defense mechanism through increased numbers of raphides (8 to 15), crystals and trichomes (13.5 to 18.2) proved acclimation of tissue culture raised plantlets from in vitro to the in vivo environments lead to 100 % success in field establishment of the plantlets. The in vitro induced foliar abnormalities (changes in stomata, venation pattern, vein density, trichomes, crystals etc.) were repaired while hardening of plantlets in the greenhouse and finally in the field. The observed micromorphological response of leaves under altered environmental conditions could help in determination of proper stage of field transfer and prediction of survival percentage of in vitro regenerated O. umbellata plantlets.

• Korean Journal of Plant Taxonomy
• /
• v.31 no.3
• /
• pp.267-282
• /
• 2001

To examine the leaf epidermal microstructure of three genera (Scopolia s.s., Anisodus, AtroPanthe, including Przewalskia as an outgroup) in the genera Scopolia Jacq. s.l., leaves of 10 species (37 specimens) were investigated by the light microscopy (LM) and scanning electron microscopy (SEM). The stomata of studied taxa were 'amphistomatic type' and the size (guard cell) range was $18-64{\times}11-48{\mu}m$. The size of stomata is slightly differed from between the taxa; the smallest size of stomata were found in the monotypic genus, Przewalskia ($24-27{\times}16-17{\mu}m$), on the other hand the largest one was found in Anisodus carniolicoides ($62-64{\times}43-48{\mu}m$). The stomatal complex was mostly anomocytic (in Scopolia s.s., Anisodus taxa : A. luridus, A. carniolicoides, A. acutangulus) and sometimes anisocytic (in Anisodus tanguticus, Przewalskia, Atropanthe). The stomata is mostly crescent in shape, but rarely circular, especially in Przewalskia tangutica. The shapes of epidermal cells are similar in both adaxial and abaxial sides, and mostly undulate/sinuate polygonal anticlinal wall, but rarely arched in Przewalskia tangutica. The epicuticular wax was not well developed in most studied taxa, except Anisodus tanguticus which is well developed cuticular striae around the stomatal complex. The elongate-headed glandular trichomes were found in Scopolia s.s. and Przewalskia. While the taxa of Anisodus and Atropanthe have not any trichomes (i. e., glabrous), except Anisodus luridus, which has simple or sometimes branched (dendritic- type) non-glandular trichome. Finally, the systematic and ecological significance of the leaf micromorphological features (stomata complex, trichome, etc.) in identification and elucidation of Scopolia s.l. including Przewalskia, especially between or within the genera including among the species is also discussed.

• Journal of the Korean Institute of Landscape Architecture
• /
• v.46 no.2
• /
• pp.62-68
• /
• 2018

This study investigated the effect of light intensity on the removal of particulate matter by Dieffenbachia amoena 'Marianne' and Spathiphyllum spp.. An acrylic chamber ($600{\times}800{\times}1200mm$, $L{\times}W{\times}H$) modeled as an indoor space and a green bio-filter ($495{\times}495{\times}1000mm$, $L{\times}W{\times}H$) as an air purification device were made of acrylic. The removal of particulate matter PM10 and PM1, the photosynthetic rate, stomatal conductance, and number of stomata of Dieffenbachia amoena 'Marianne' and Spathiphyllum spp. were measured according to three different levels of light intensity (0, 30 and $60{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}PPFD$). Regarding the length of time taken for PM10 to reach $1{\mu}g$, the Dieffenbachia amoena 'Marianne' showed a significant difference according to the presence or absence of light, and there was no significant difference shown between light intensity of 30 and $60{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}PPFD$. As for the Spathiphyllum spp., there was no significant difference between 0 and $30{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}PPFD$, while a significant difference was shown at $60{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}PPFD$. After 90 minutes, the PM1, PM10, and $CO_2$ residuals of the Spathiphyllum spp. were lowest at $60{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}PPFD$. The remaining amount of PM1 and PM10 was lower with the Spathiphyllum spp. than with the Dieffenbachia amoena 'Marianne', even at $0{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}PPFD$. With both plants, the higher the light intensity, the higher the photosynthetic rate, while the stomatal conductance did not show any significant difference. Spathiphyllum spp. showed a higher photosynthetic rate and stomatal conductance and a greater number of stomata than Dieffenbachia amoena 'Marianne', and stomata were observed in both the front and back sides of the leaves. The air purification effect of Spathiphyllum spp. is considered to be better than Dieffenbachia amoena 'Marianne' at the same light intensity due to such plant characteristics. Therefore, in order to select effective indoor plants for the removal of particulate contamination in an indoor space, the characteristics of plants such as the photosynthetic rate and the number and arrangement of stomata according to indoor light intensity should be considered.

• Korean Journal of Plant Taxonomy
• /
• v.48 no.2
• /
• pp.143-152
• /
• 2018

A comparative study of leaf epidermal microstructures in genus Aruncus (two species, five varieties) was carried out using scanning electron microscopy in order to evaluate their significance in terms of taxonomy. All of the leaves of the taxa studied here were amphistomatic with undulate anticlinal walls, and smooth and flat periclinal walls on both surfaces. The size range of the stomata complex is $8.95-21.97{\times}7.50-16.99{\mu}m$: the largest one was found in Aruncus dioicus var. astilboides (average $18.01{\times}13.47{\mu}m$) and the smallest was measured and determined to be A. gombalanus (average $11.11{\times}8.94{\mu}m$). An anomocytic stomata complex was found in all of the studied taxa. The stomatal frequency on average was $27.54/0.05mm^2$; it is highest in A. gombalanus ($60.4/0.05mm^2$) and lowest in A. dioicus var. acuminatus ($11.6/0.05mm^2$). Two types (short stalked capitate glandular trichome and non-glandular trichome) of trichomes are found in the leaves. The non-glandular trichome was divided into three types based on the presence and degree of development of subsidiary cells. Anomocytic stomata of the hypostomatic type and the distribution pattern of capitate glandular trichomes were the major characters in this genus. The stomata size and frequency, the epidermal cell structure, the trichome type and the distribution pattern may have diagnostic importance among the taxa in the genus. Our leaf micromorphological results provide useful information for the taxonomic revision of the genus Aruncus.

• Horticultural Science & Technology
• /
• v.29 no.6
• /
• pp.617-622
• /
• 2011

The study was conducted to determine the efficiency in producing spontaneous polyploids in some mandarin hybrids with different seed embryony. Seed formation by open pollination, frequency of spontaneous polyploids, and plant growth characteristics were evaluated in four mandarin hybrids with polyembryony such as 'Amakusa', 'Haruka', 'Hayaka', and 'Seminole' and two with monoembryony such as 'Benibae' and 'Harehime'. The mean number of the developed seeds per fruit was 10.0 and frequency of small seeds was 25.1%. Polyploids were selected from plants germinated in vitro by a flow cytometry and confirmed by chromosome analysis. One triploid was produced from 'Harehime', one tetraploid, 'Amakusa', and one tetrapoid, 'Benibae'. There were little differences in leaf shape, thickness, petiole length, and internode length between diploids and polyploids such as tri- or tetraploid. However, polyploids had larger stomata and lower density of stomata in abaxial epidermis than diploids. SPAS indicating chlorophyll content and photosynthetic rate were significantly affected by ploidy level. The results indicated that spontaneous polyploids might be produced by open pollination in some mandarin hybrids and monoembryony had higher frequency in polyploid occurrence than polyembryony.

• Journal of Forest and Environmental Science
• /
• v.34 no.3
• /
• pp.224-234
• /
• 2018

An efficient method to select drought tolerant Korean native plants using in vitro culture system was established in this study. While the plant growths and root inductions of each plant were proportionately affected by concentrations of mannitol on in vitro culturing seven plant species to test tolerance to osmotic stress, growth index (GI) and number of root induction of Chrysanthemi zawadskii var. latilobum and Dianthus chinensis var. semperflorens plantlets were higher than the others in 125mM mannitol. In test with polyethylene glycol (PEG), plantlets of C. zawadskii var. latilobum and D. chinensis var. semperflorens showed higher GI and number of root induction than the others in 33.3mM. On testing whether the well grown plants under osmotic stress are tolerant to virtual drought stress, there were significant differences in the withering rates of C. zawadskii var. latilobum and D. chinensis and those of were Aster yomena and Centaurea cyanus after 12 days without watering. It was found that significantly lower stomata numbers were shown in both drought tolerant plants than the sensitive plants. Averages of the stomata circumferences and the stomata area in the plantlets of the tolerant species were larger than those of the sensitive plants D. chinensis var. semperflorens showed the lowest transpiration level per unit area. The highest stomatal area per unit area was found in C. zawadskii, followed by D. chinensis var. semperflorens, Aster yomena and C. cyanus. In conclusion, C. zawadskii var. latilobum and D. chinensis var. semperflorens were more tolerant to drought than other two species. Furthermore in vitro selection was successfully used to screen drought tolerance species of native plant species.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.27 no.2
• /
• pp.130-136
• /
• 1982

Experiments were conducted to establish a measuring method of the stomatal aperture in the leaves of the wheat plant, and to find the diurnal movements of the stomate in leaves of different position and tillers. The measurement methods used were the infiltration and the microrelief impression methods. The aperture of the stomate in the infiltration method was expressed in terms of the solutions pent ration into the leaf and this was refered to as the infiltration score. A score I represents injection with 10% iso-butyl alcohol＋90% ethylene glycol solution and a score 7 represents injection with 70% iso-butyl alcohol +30% ethlene glycol solution. A linear relationship was obtained between the infiltration score and average pore width in a large number. of the stomata observed in the leaves of the adaxial and abaxial epidermis. The aperature of the stomate of flag and the 1st leaf were exhibited diurnal change with the maximum aperture at 10 A.M. but that of 2nd leaf reached maximum aperture 2 hours later than upper two leaves. After reaching the maximum aperture the stomata gradually closed and then completely closed at 6 P.M. The aperture of the stomate in the adaxial epidermis and the base part of the leaf were larger than those in the abaxial and top part of the leaf, and aperture of the stomate in the leaves of the main stem was larger than those on the tillers.