• Korean Journal of Veterinary Research
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• v.41 no.4
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• pp.477-484
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• 2001

The regional distribution and relative frequency of neurohormonal peptides-producing cells were demonstrated in the gut of the stomachless teleost, the Prussian carp, Carassius auratus Linnaeus, using 10 types of specific antisera raised against mammalian regulatory peptides. The gut of the Prussian carp was divided into five portions from proximal to distal (Segments I~V). Most of immunoreactive cells in the epithelial lining portion, between epithelial cells, were generally spherical or spindle shape having long cytoplasmic process that reached to the lumen (open typed cell) while cells showing round in shape (close typed cell) were found in the basal portions of epithelial lining occasionally. Somatostatin-, cholecystokinin (CCK)-8- and pancreatic polypeptide (PP)- immunoreactive cells were observed in this study. However, no serotonin-, glucagon-, chromogranin A-, secretin-, vasoactive intestinal peptide (VIP)-, substance P- and bombesin-immunoreactive cells were found. Somatostatin-immunoreactive cells were restricted to most proximal segments of the gut (Segment I) with rare frequency and CCK-8-immunoreactive cells were demonstrated in the proximal segments of the gut (Segments I and II) with a few to rare frequencies. In addition, pancreatic polypeptide-immunoreactive cells were demonstrated in the proximal to middle segments (Segments I~III) with moderated to rare frequencies. In conclusion, the distribution and relative frequency of these immunoreactive cells are well corresponded to the previous reports in stomachless teleost but somewhat peculiar patterns are also detected.

• Korean Journal of Veterinary Research
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• v.42 no.3
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• pp.289-297
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• 2002

The regional distribution and relative frequency of neurohormonal peptides-producing cells were demonstrated in the gut of the stomach teleost, the Mandarin fish, Siniperca scherzeri Steindachner, using 7 types of specific antisera raised against mammalian regulatory peptides. The gastrointestinal tract of the Mandarin fish was divided into three portions from proximal to distal, stomach, small intestine and large intestine. Cells showing immunoreactivities against regulatory peptides were situated in the epithelial lining, between epithelial cells, and gastric or intestinal gland regions with various frequencies along with gastrointestinal tract. Mast of immunoreactive cells in the epithelial lining portion were generally spherical or spindle shape having long cytoplasmic process that were reached to the lumen (open type cell) while cells showing round in shape (closed type cell) were found in the gastric gland of the stomach occasionally. Serctonin-, samatostatin-, gastrin-, cholecystokinin (CCK)-8- and human pancreatic polypeptide (HPP)-immunoreactive cells were observed in this study. However, no insulin- and glucagon-immunoreactive cells were found. Serotonin- and somatostatin-immunoreactive cells were restricted to the stomach regions with moderate and numerous frequencies, respectively. Gastrin-immunoreactive cells were demonstrated in the stomach and small intestinal portions with a few and moderate frequencies, respectively and CCK-8-immunoreactive cells were restricted to the small intestinal portions with moderate frequency. In addition, HPP-immunoreactive cells were demonstrated in the stomach and small intestine with numerous frequencies, respectively. In conclusion, the distribution and relative frequency of these immunoreactive cells in the gastrointestinal tract of the Mandarin fish shows peculiar patterns compared to those of other stomach and/or stomachless teleost.