Browse > Article

An Immunohistochemical Study on the Endocrine Cells in the Gastrointestinal Tract of the Mandarin Fish (Siniperca scherzeri)  

Lee, Jae-Hyun (Department of Histology, College of Veterinary Medicine, Kyungpook National University)
Ku, Sae-Kwang (Pharmacology & Toxicology Lab., Central Research Laboratories, Dong-Wha Pham. Ind. Co.)
Park, Ki-Dae (Department of Histology, College of Veterinary Medicine, Kyungpook National University)
Lee, Hyeung-Sik (Department of Biology. Faculty of Natural Sciences, Kyungsan University)
Publication Information
Korean Journal of Veterinary Research / v.42, no.3, 2002 , pp. 289-297 More about this Journal
Abstract
The regional distribution and relative frequency of neurohormonal peptides-producing cells were demonstrated in the gut of the stomach teleost, the Mandarin fish, Siniperca scherzeri Steindachner, using 7 types of specific antisera raised against mammalian regulatory peptides. The gastrointestinal tract of the Mandarin fish was divided into three portions from proximal to distal, stomach, small intestine and large intestine. Cells showing immunoreactivities against regulatory peptides were situated in the epithelial lining, between epithelial cells, and gastric or intestinal gland regions with various frequencies along with gastrointestinal tract. Mast of immunoreactive cells in the epithelial lining portion were generally spherical or spindle shape having long cytoplasmic process that were reached to the lumen (open type cell) while cells showing round in shape (closed type cell) were found in the gastric gland of the stomach occasionally. Serctonin-, samatostatin-, gastrin-, cholecystokinin (CCK)-8- and human pancreatic polypeptide (HPP)-immunoreactive cells were observed in this study. However, no insulin- and glucagon-immunoreactive cells were found. Serotonin- and somatostatin-immunoreactive cells were restricted to the stomach regions with moderate and numerous frequencies, respectively. Gastrin-immunoreactive cells were demonstrated in the stomach and small intestinal portions with a few and moderate frequencies, respectively and CCK-8-immunoreactive cells were restricted to the small intestinal portions with moderate frequency. In addition, HPP-immunoreactive cells were demonstrated in the stomach and small intestine with numerous frequencies, respectively. In conclusion, the distribution and relative frequency of these immunoreactive cells in the gastrointestinal tract of the Mandarin fish shows peculiar patterns compared to those of other stomach and/or stomachless teleost.
Keywords
Immunohistochemistry; gastrointestinal tract; Mandarin fish; teleost; regulatory peptides; Siniperca scherzeri;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Cheung R, Andrews PC, Plisetskaya EM, et al. Immunoreactivity to peptides belonging to the pancreatic polypeptide family (NPY, dPY, PP, PYY) and to glucagon-like peptide in the endocrine pancreas and anterior intestine of adult lampreys, Petromyzon marinus: an immunohistochemical study. Gen Camp Endocrinol, 81:51-63, 1991
2 Tagliafierro G, Carlini M, Faraldi G, et al. Immunocytochemical detection of islet hormones in the digestive system of Protopterus annectens. Gen Comp Endocrinol, 102:288-298, 1996
3 Tagliafierro G, Bonini E, Faraldi G, et al. Distribution and ontogeny of VIP-like immunoreactivity in the gastro-entero-pancreatic system of a cartilaginous Fish Scyliorhinus stellaris. Cell Tissue Res, 253: 23-28, 1988
4 Reinecke M, Muller C, Segner H. An immunohistochemical analysis of the ontogeny, distribution and coexistence of 12 regulatory peptides and serotonin in endocrine cells and nerve fibers of the digestive tract of the turbot, Scophthalmus maximus (Teleostei). Anat Embryol (Berl), 195:87-101, 1997
5 Groff KE, Youson JH. An immunohistochemical study of the endocrine cells within the pancreas, intestine, and stomach of the gar (Lepisosteus osseus L.). Gen Comp Endocrinol, 106:1-16, 1997
6 Jonsson AC, Holmgren S, Holstein B. Gastrin/CCK-like immunoreactivity in endocrine cells and nerves in the gastrointestinal tract of the cod, Gadus morhua, and the effect of peptides of the gastrin/CCK family on cod gastrointestinal smooth muscle. Gen Comp Endocrinol, 66:190-202, 1987
7 Beorlegui C, Martinez A, Sesma P. Endocrine cells and nerves in the pyloric ceca and the intestine of Oncorhynchus mykiss (Teleostei): an immunocytochemical study. Gen Comp Endocrinol, 86:483-495, 1992
8 Andreozzi G, de Girolamo P, Affatato C, et al. VIP-like immunoreactivity in the intestinal tract of fish with different feeding habits. Eur J Histochem, 41:57-64, 1997
9 Pan QS, Fang ZP. An immunocytochemical study of endocrine cells in the gut of a stomachless teleost fish, grass carp, Cyprinidae. Cell Transplant, 2:419-427, 1993
10 Reinecke M. Substance P is a vasoactive hormone in the Atlantic hagfish Myxine glutinosa (Cyclostomata). Gen Comp Endocrinol, 66:291-296, 1987
11 Franchini A, Rebecchi B, Bolognani Fantin AM. Gill endocrine cells in the goldfish Carassius carassius var. auratus and their impairmeat following experimental lead intoxication. Histochem J, 31:559-564, 1999
12 Sternberger LA. The unlabeled antibody peroxidase-antiperoxidase (PAP) method. In: Sternberger LA (ed), Immunocytochemistry, John Wiley & Sons, New York, pp 104-169, 1979
13 Kobayashi S, Fujita T, Sasagawa T. Electron microscope studies on the endocrine cells of the human gastric fundus. Arch Wistol Jap, 32:429-444, 1971
14 D'Este L, Buffa R, Pelagi M, et al. Immunohistochemical localization of chromogranin A and B in the endocrine cells of the alimentary tract of the green frog, Rana esculenta. Cell TIssue Res, 277:341-349, 1994
15 Marsiani E. Lebow LT, Rozga J, et al. Teleost fishislet: a potential source of endocrine tissue for the treatment of diabetes. J Surg Res, 58:583-591, 1995
16 Rombout JH, Reinecke M. Immunohistochenncal localization of (neuro)peptide hormones in endocrine cells and nerves of the gut of stomachless teleost fish, Barbus conchonius (Cyprinidae). Cell Tissue Res, 237:57-65, 1984
17 Faraldi G, Bonini E, Farina L, et al. Distribution and ontogeny of glucagon-like cells in the gastrointestinal tract of the cartilaginous fish Scyliorhinus stellaris (L.). Acta Histochem, 83:57-64, 1988
18 Youson JH, Potter IC. An immunohistochemical study of enteropancreatic endocrine cells in larvae and juveniles of the southern-hemisphere lampreys Geotria australis and Mordacia mordax. Gen Comp Endocrinol, 92:151-167, 1993
19 Kiliaan A, Holmgren S, Jonsson AC, et al. Neurotensin, substance P, gastrin/cholecystokinin, and bombesin in the intestine of the tilapia (Oreochromis mossambicus) and the goldfish (Carassius auratus): immunochemical detection and effecfs on electrophysiological characteristics. Gen Comp Endocrinol, 88:351-363, 1992
20 Alumets J, Sundler F, Hakanscn R. Distribution, ontogeny and ultrastructure of somatostatin immunoreactive cells in the pancreas and gut. Cell Tissue Res, 186:467-479, 1977
21 Chiba A. Ontogeny of serotonin-immunoreactive cells in the gut epithelium of the cloudy dogfish, Scyliorhinus torazame, with reference to coexistence of serotonin and neuropeptide Y. Gen Comp Endocrinol, 111:290-298, 1998
22 Falkmer S, Van Noorden S. Ontogeny and phylogeny of glucagon cell. Handb Exp Pharmacol, 66:81-119, 1983
23 Domeneghini C, Radaelli G, Arrighi S, et al. Neurotrransmitters and putative neuromodulators in the gut of Anguilla anguilla (L.). Localizations in the enteric nervous and endocrine systems. Eur J Histochem, 44:295-306, 2000
24 Sarasquete MC, Polo A, Gonzalez de Canales ML. A histochemical and immunohistochemical study of digestive enzymes and hormones during the larval development of the sea bream, Sparus aurata L. Histochem J, 25:430-437, 1993
25 Lee HS, Ku SK, Lee JH. An immunohistochemical study of endocrine cells in the gut of the Prussian carp, Carassius auratus. Korean J Vet Res, 41:477-484, 2001
26 Zaccone G, Fasulo S, Ainis L. Distribution patterns of the paraneuronal endocrine cells in the skin, gills and the airways of fishes as determined by immunohistochemical and histological methods. Histochem J, 26:609-629, 1994
27 Cimini V, van Noorden S, Sansone M. Neuropeptide Y-like immunoreactivity in the dogfish gastroenteropancreatic tract: light and electron microscopical study. Gen Comp Endocrinol, 86:413-423, 1992
28 Al-Mahrouki AA, Youson JH. Immunohistochemical studies of the endocrine cells within the gastro-entero-pancreaic system of Osteoglossomorpha, an ancient teleostean group. Gen Camp Endocrinol, 110:125-139, 1998
29 Yui R, Nagata Y, Fujita T. Immunocytochemical studies on the islet and the gut of the arctic lamprey, Lampetra japonica. Arch Histol Cytol, 51:109-119, 1988
30 Conlon JM, Falkmer S. Neurohormonal peptides in the gut of the Atlantic hagfish (Myxine glutinosa) detected using antisera raised against manimalian regulatory peptides. Gen Comp Endocrinol, 76:292-300, 1989
31 Kurokawa T, Suzuki T, Andoh T. Development of cholecystokinin and pancreatic polypeptide endocrine systems during the laval stage of Japanese flounder, Paralichthys olivaceus. Gen Comp Endocrinol, 120:8-18, 2000
32 Nozaki M, Miyata K, Oota Y, et al. Colocalization of glucagon-like peptide and glucagon immunoreactivities in the pancreatic islets and intestine of salmonids. Cell Tissue Res, 253:371-375, 1988
33 Brazeau P, Vale W, Burgurs R, et al. Hypothalamic polypeptide that inhibits the secretion of immunoreactive pituitary growth harmone. Science, 179:77-79, 1973
34 Solcia E, Capella C, Vassallo G, et al. Endocrine cells of the gastric mucosa. Int Rev Cytol, 42:223-286, 1975
35 Lee JH, Ku SK, Park KD, et al. Comparative study of endocrine cells in the principal pancreatic islets of two teleosts, Silurus asotus (Siluridae) and Siniperca scherzeri (Centropomidae). J Vet Sci, 2:75-80, 2001
36 Solcia E, Usellini L, Buffa R, et al. Endocrine cells producing regulatory peptides. In: Polak JM (ed), Regulatory peptides. Birkhauser, Basel, pp 220-246, 1989
37 De Girolamo P, Lucini C, Vega JA, et al. Colocalization of neurotrophin receptors and regulatory peptides in the endocrine cells of the teleostean stomach. Anat Rec. 256:219-26, 1999
38 El-Salhy M, Winder E, Lundqvist M. Comparative studies of serotonin-like immunoreactive cells in the digestive tract of vertebrates. Biomed Res, 6:371-375, 1985
39 Elbal MT, Lozano MT, Agulleiro B. The endocrine cells in the gut of Mugil saliens Risso, 1810 (Teleostei): and immunocytochemical and ultrastructural study. Gen Comp Endocrinol, 70:231-246, 1988
40 Holmgren S, Nilsson S. Bombesin-, gastin/CCK-, 5-hydroxytryptanmie-, neurotensin-, somatostatin-, and VIP-like immunoreactivity and catecholamine fluorescence in the gut of the elasmobranch, Squalus acanthias. Cell Tissue Res, 234:595-618, 1983
41 Pederzoli A, Trevisan P, Bolognani Fantin AM. Immunocytochemical study of endocrine cells in the gut of goldfish Carassius carassius (L.) var auratus submitted to experimental lead intoxication. Eur J Histochem, 40:305-314, 1996
42 Pan QS, Fang ZP, Huang FJ. Identification, localization and morphology of APUD cells in gastroenteropancreatic system of stomach-containing teleosts. World J Gastroenteiol, 6:842-847, 2000
43 Chiba A, Honma Y, Oka S. Ontogenetic development of neuropeptide Y-like-immunoreactive cells in the gastroenteropancreatic endocrine system of the dogfish. Cell Tissue Res, 282:33-40, 1995
44 Gabe M. Donn es histologiques sur les cellules endocrines gastroduodenales des amphibiens. Arch Histol Jap, 35:51-81, 1972
45 Bell FR. The relevance of the new knowledge of gastrointestinal hormones to veterinary science. Vet SciCommun, 2:305-314, 1979