• 현장농수산연구지
• /
• 제4권1호
• /
• pp.65-69
• /
• 2002

버섯수량성에 미치는 오존의 영향을 조사한 결과는 다음과 같다. 1. 느타리버섯 생육과정중 오존처리 결과, 버섯 대길이는 짧아지고, 대와 갓 직경은 커지는 경향이 있으며 수량은 관행에 비하여 7% 증수하였다. 2. 양송이 생육과정중 오존처리 결과, 버섯 대 길이는 짧아지고, 대와 갓 직경은 커지는 경향이 있으며 수량은 관행에 비하여 4% 증수하였다. 3. 팽이버섯 생육과정중 오존처리결과, 초발이 소요일수가 2일 늦어지고, 발이 개체수가 적었으나 수량은 3% 증수하였다.

• 한국수산과학회지
• /
• 제17권6호
• /
• pp.492-498
• /
• 1984

We have reported the seasonal and portional variation in the composition of uronic acid of alginic acid from Ecklonia cava in the previous study. In the present paper, uronic acid block structure of alginic acid from Ecklonia cave was investigated, and some related properties such as viscosity, the dependence on temperature and substitution of metallic ion were also discussed. The proportion M block was highest amnog three blocks in both of frond and stipe. The average values of M and G block ratios were $51.6|%\;and\;32.3\%$ in the frond, whereas $45.7\%\;and\;38.6\%$ in the stipe, respectively. The proportions of alternating blocks in the frond and stipe were similar, and the values were $16.1\%$ in the frond and $15.6\%$ in the stipe. The viscosity of $1\%$ sodium alginate solution was almost inversely proportional to the M/G and/or M block ratio. Viscosities were marked maximum from summer to autumn and minimum winter to spring. In the value of lower M/G and M block ratio, the viscosity seemed to have a considerable dependence on temperature. The temperature dependence was more appearant in the alginic acid from the frond (M/G ratio, 0.95; M block, $54.2\%$) collected in July and the stipe (M/G ratio, 1.61; M block $47.6\%$) in April. The affinity with metallic ion appeared higher in order of $Pb^{2+}>Cu^{2+}>Co^{2+}>Zn^{2+}$. The amounts of metallic ion exchange of the $Pb^{2+},\;Cu^{2+},\;Co^{2+}\;and\;Zn^{2+}$ were ranged from 3.4. to 4.7, 2.5 to 3.2, 1.8 to 2.2 and 1.6 to 1.7 meq/(g. sodium alginate), respectively.

• Journal of Microbiology and Biotechnology
• /
• 제28권4호
• /
• pp.622-629
• /
• 2018

Expansins are cell wall proteins that mediate cell wall loosening and promote specific tissue and organ morphogenesis in plants and in some microorganisms. Unlike plant expansins, the biological functions of fungal expansin-like proteins have rarely been discussed. In the present study, an expansin-like protein-encoding fvexpl1 gene, was identified from Flammulina velutipes by using local BLAST. It consisted of five exons with a total length of 822 bp. The deduced protein FVEXPL1 contained 274 amino acids with a predicted molecular mass and isoelectric point of 28,589 Da and pH 4.93, respectively. The first 19 amino acids from the N terminal are the signal peptide. Phylogenetic analysis and multiple protein alignment indicated FVEXPL1 was an expansin-like protein. The expression level of fvexpl1 gene in the stipe was significantly higher than that in the mycelia, primordia, and cap. However, the expression level of fvexpl1 gene was significantly higher in the fast elongation region of the stipe as compared with the slow elongation region. Expression analysis indicated that fvexpl1 gene might have an auxiliary role in the stipe morphogenesis of F. velutipes.

• Fisheries and Aquatic Sciences
• /
• 제24권5호
• /
• pp.197-206
• /
• 2021

Marine brown seaweeds are a source of functional ingredients with various biological properties. They have been used in the food and functional food industries. Brown seaweeds are divided into three parts of blades, stipe, and root. Normally seaweed blades were used as raw materials for biological research. However, there are limited uses on stipes of Ecklonia maxima (E. maxima) depending on the physicochemical, nutritional, and biological properties. Besides, the comparative studies of two structures of E. maxima, blades and stipe didn't discover previously. This study aimed to compare the potent antioxidant and anti-inflammatory activities of the two structures of E. maxima, blades and stipe in vitro studies to increase the utilization of the two structures of E. maxima. The enzyme-assisted hydrolysate from E. maxima showed significant antioxidant and anti-inflammatory activities. Among them, celluclast-assisted hydrolysate from E. maxima blades (EMBC) and viscozyme-assisted hydrolysate from E. maxima stipe (EMSV) expressed significant protection on hydrogen peroxide-induced oxidative stress. Moreover, EMBC and EMSV treatment remarkably reduced nitric oxide production by downregulation of pro-inflammatory cytokine expressions in lipopolysaccharide-stimulated Raw 264.7 cells. Especially EMBC showed strong inhibition on pro-inflammatory cytokine production compared to EMSV. Taken together research findings suggest that EMBC and EMSV possessed potent antioxidant and anti-inflammatory properties and may be utilized as functional ingredients in the food and functional food sectors.

• 현장농수산연구지
• /
• 제12권1호
• /
• pp.21-27
• /
• 2010

The objective of this study was to determine the effects of "Green-one" organic nutrient on mycelial growth and fruiting of P. ostreatus. The dilution concentrations of "Green-one" was treated as follows. There was control, 100, 200, 400 concentrations. That treatments were treated with step of each mycelial growth step. The best of growth steps was mycelial scratching step. At that time, DPI(Day required for primordial formation after inoculation) was shortened for 1 day. Valid germination stipe are 15 pieces, 3 pieces more than control. Stipe length and stipe diameter was long each 4mm, 3mm more than control. Pileus size is shortened than control. Yields per one bottle(g/850cc) was 146g increased 6.5% than control 137g/850cc.

• 한국식품과학회지
• /
• 제22권7호
• /
• pp.828-832
• /
• 1990

표고버섯의 갓과 자루부위의 품질상태를 비교하고 에탄올 농도와 추출시간을 달리하여 extract를 제조한 후 수율, 환원당, 유리아미노산, 핵산관련물질을 조사하였다. 추출용매 및 추출시간의 변화에 따른 이들 함량의 변화를 보면 수율은 갓과 자루부위 모두 열수추출시 다소 높았고 환원당은 열수추출시 갓의 경우 4시간에서 488.18 mg%, 자루의 경우 3시간에서 452.12 mg%로서 나타났다. 유리아미노산은 부위에 관계없이 갓과 자루에서 각각 2시간, 3시간 열수추출시 528.46, 221.01 mg%로 가장 높았으며 고미성 아미노산은 갓의 경우25% 에탄올 용액으로 5시간 추출시 29%를 차지하였으며, 자루의 경우 50% 에탄올을 이용시 37%에 달했다. 핵산관련물질의 함량은 자루부위보다 갓부위에서 높게 나타났으며 추출용매의 농도는 25% 에탄올에서 부위에 관계없이 그 추출율이 가장 높았다.

• Mycobiology
• /
• 제47권4호
• /
• pp.441-448
• /
• 2019

Two new SAM-dependent methyltransferase encoding genes (fvsmt1 and fvsmt2) were identified from the genome of Flammulina velutipes. In order to make a comprehensive characterization of both genes, we performed in silico analysis of both genes and used qRT-PCR to reveal their expression patterns during the development of F. velutipes. There are 4 and 6 exons with total length of 693 and 978 bp in fvsmt2 and fvsmt1, respectively. The deduced proteins, i.e., FVSMT1 and FVSMT2 contained 325 and 230 amino acids with molecular weight 36297 and 24894 Da, respectively. Both proteins contained a SAM-dependent catalytic domain with signature motifs (I, p-I, II, and III) defining the SAM fold. SAM-dependent catalytic domain is located either in the middle or at the N-terminal of FVSMT2 and FVSMT1, respectively. Alignment and phylogenic analysis showed that FVSMT1 is a homolog to a protein-arginine omega-N-methyltransferase, while FVSMT2 is of cinnamoyl CoA O-methyltransferase type and predicted subcellular locations of these proteins are mitochondria and cytoplasm, respectively. qRT-PCR showed that fvsmt1 and fvsmt2 expression was regulated in different developmental stages. The maximum expression levels of fvsmt1 and fvsmt2 were observed in stipe elongation, while no difference was found in mycelium and pileus. These results positively demonstrate that both the methyltransferase encoding genes are involved in the stipe elongation of F. velutipes.

• 미생물학회지
• /
• 제51권2호
• /
• pp.148-155
• /
• 2015

세균 16S rRNA 유전자 특이 프라이머를 기초로 하여 중합 효소연쇄반응을 이용하여 Pleurotus eryngii (큰느타리 버섯)의 내부에 존재하는 세균의 다양성을 조사하였다. 세균 16S rRNA의 라이브러리는 버섯 갓(BC, body cap)와 줄기(BS, body stipe)로 구성하였다. BC 20 클론은 네 그룹으로 구별되었고 가장 큰 그룹은 Firmicutes (클론의 40%) 있었다. 그러나, BS 20 클론은 여섯 그룹으로 나뉘어졌고 가장 큰 그룹은 Actinobacteria (클론의 40%) 있었다. 전체 버섯 내에 존재하는 세균 그룹은 그람양성 세균(62.5%) 있었다.

• Mycobiology
• /
• 제49권6호
• /
• pp.589-598
• /
• 2021

White strains of Hypsizygus marmoreus are more difficult to cultivate than are brown strains; therefore, new white strain breeding strategies are required. Accordingly, we constructed the genetic map of H. marmoreus with 1996 SNP markers on 11 linkage groups (LGs) spanning 1380.49 cM. Prior to analysis, 82 backcrossed strains (HM8 lines) were generated by mating between KMCC03106-31 and the progenies of the F1 hybrid (Hami-18 × KMCC03106-93). Using HM8, the first 23 quantitative trait loci (QTLs) of yield-related traits were detected with high limit of detection (LOD) scores (1.98-9.86). The length, thickness, and hardness of the stipe were colocated on LG 1. Especially, length of stipe and thickness of stipe were highly correlated given that the correlation coefficients were negative (-0.39, p value ≤ .01). And a typical biomodal distribution was observed for lightness of the pileus and the lightness of the pileus trait belonged to the LG 8, as did traits of earliness and mycelial growth in potato dextrose agar (PDA) medium. Therefore, results for color traits can be suggested that color is controlled by a multi-gene of one locus. The yield trait was highly negatively correlated with the traits for thickness of the stipe (-0.45, p value ≤ .01). Based on additive effects, the white strain was confirmed as recessive; however, traits of mycelial growth, lightness, and quality were inherited by backcrossed HM8 lines. This new genetic map, finely mapped QTLs, and the strong selection markers could be used in molecular breeding of H. marmoreus.

• 한국버섯학회지
• /
• 제12권1호
• /
• pp.67-72
• /
• 2014

유통 중인 큰느타리버섯과 재배농가에서 출하되고 있는 버섯의 등급별 품질규격을 조사하여 농산물표준규격의 내용을 개선하고 재배농가에서 고품질의 큰느타리버섯 생산을 위한 품질관기 기준을 제시하고자 하였다. 가락동시장에서 구입한 큰느타리버섯의 경우 A등급(특품)의 평균개체중은 91.2 g, 갓 직경은 60.1 mm, 대 길이는 88.3 mm였으며, B등급(상품)의 개체중은 68.5g, 갓 직경은 63.8 mm, 대 길이는 86.6 mm였다. A등급(특품)의 경우 대길이/대굵기 비율이 1.7, 갓직경/대굵기 비율이 1.1~1.2였으며, B등급(상품)의 경우 대길이/대굵기 비율이 1.9~2.1, 갓직경/대굵기 비율이 1.4~1.7였으나, C등급(보통)의 경우는 서로간에 상관관계가 없었다. 농가에서 구입한 큰느타리버섯의 경우 A등급(특품)으로 분류된 버섯의 평균개체중은 101.8 g, 대굵기는 53.8 mm, 대길이/대굵기 비율은 1.6~1.7, 갓직경/대굵기 비율은 1.01.2였으며, B등급(상품)의 경우 평균개체중은 77.8 g, 대굵기는 45.3 mm, 대길이/대굵기 비율이 1.8~1.9, 갓직경/대굵기 비율이 1.2~1.4였다. 따라서 고품질의 큰느타리버섯 생산을 위하여 농가에서 활용 가능한 품질관리 기준을 다음과 같다. A등급(특품)의 기준은 평균개체중은 90 g 이상, 대굵기는 50 mm 이상, 대길이/대굵기 비율은 1.6~1.7, 갓직경/대굵기 비율은 1.1~1.2이 되어야 하며, B등급(상품)은 평균개체중은 45 g 이상, 대굵기는 40 mm 이상, 대길이/대굵기 비율이 1.8~1.9, 갓직경/대굵기 비율이 1.2~1.4가 될 경우 가락동시장에서 특품과 상품으로 판매가 가능할 것으로 판단된다.