• Journal of Applied Biological Chemistry
• /
• 제57권1호
• /
• pp.61-63
• /
• 2014

Aged black garlic (ABG) was extracted with 20% ethanol and water (crude extracts) and fractionated into three categories (>10, 3-10, and <3 kDa). The effect of crude extract supplements on anti-My-10 hybridoma cell growth and IgG1 antibody production was investigated in suspension culture with a chemically defined protein-free medium. We observed that supplementation of ABG to the cell culture medium stimulated anti-My-10 hybridoma cell growth and production of IgG1 antibody, particularly with fractionated ABG of low molecular weight. The stimulation depended upon the concentration and the size of the fractionated ABG. We also found that the growth-promoting activity was not correlated with high antibody production. These results suggest that fractionated ABG is a novel and promising alternative as an animal cell culture supplement.

• Journal of Nutrition and Health
• /
• 제28권11호
• /
• pp.1065-1072
• /
• 1995

Mugwort was successively fractionated with n-hexane, chloroform, ethyl acetate, n-butanol and water and the fractions were evaluated by their growth-promoting activites for Bifidobacterium sp. in vitro experiments. The growths of Bifidobacterium adolescentis, B. bifidum, B. infantis and B.longum were enhanced with the addition of the water fraction, while the fractions of chloroform and ethylacetate inhibited Clostridium perfringens. When the wate fraction was added to media at a concentration of 0.01-0.5%(w/v), the growhts of Bifidobacterium sp. were increased according to the concentration of water fraction used. The water fraction stimulated also the growth of lactobacillus acidophillus, whereas those of E. coli and Enterococcus faecalis were not affected. The growth-promoting activity of water fraction was stable at the range of pH 2 to pH 10 and kept in thermal treatment at 10$0^{\circ}C$ for 30 minutes.

• The Korean Journal of Physiology and Pharmacology
• /
• 제8권6호
• /
• pp.351-354
• /
• 2004

The cytotoxicological responses to insect growth regulator (IGR), using tebufenozide as ecdysteroid mimic, were investigated in Drosophila Kc cells. Treatment of Kc cells with tebufenozide showed significant growth inhibition and striking morphological changes including aggregation and elongation of the cells. In order to understand the cellular mechanism underlying the response of Drosophila cells to tebufenozide, immunofluorescence microscopy was performed. We found that treatment of Kc cells with tebufenozide enhanced the reorganization of f-actin and stimulated the expression of hsp27. These data suggest a possible association of filamentous actin (f-actin) and hsp27 in the cytotoxicological mechanisms of growth regulators in Drosophila cells.

• 한국자원식물학회지
• /
• 제1권1호
• /
• pp.48-52
• /
• 1988

식물생장촉진제와 억제제의 처리가 동백의 화아형성과 개화기에 미치는 영향을 알아본 결과 다음과 같은 결과를 얻었다. 1. 봄철 맹어후 살포처리한 gibberellin은 shoot의 신장을 촉진시켰으나 화아형성을 억제시켰다. 2. Cycocel(CCC)의 처리는 화아형성에 촉진적으로 작용하였다. 3. 화아에 처리한 gibberellin은 개화시기를 효과적으로 단축시켰다.

• The Korean Journal of Ecology
• /
• 제23권2호
• /
• pp.169-173
• /
• 2000

Seasonal distribution of ectomycorrhizal associations in various types of forest in a boreal forest in Manitoba. Canada was investigated. Alsohe relationship between ectomycorrhizal growth and pine pollen nutrients was examined. In four different forest stands, ectomycorrhizas tended to be lower in the spring than in the summer and fall samples. In addition. a mature jack pine (Pinus banksiana) stand showed higher mycorrhizal activities than a young jack pine stand. Growth of Suillus brevipes hyphae wa ts stimulated by additions of pollen representing mean pollen deposition in Mistik Creek study area after 30 and 70 days of growth with dextrose availability. This result suggests that the peak ectomycorrhizal activity is followed by pollen deposition in the study region and therefore, addition of pine and spruce pollen in early or middle of June in the boreal forest can be an important seasonal nutrient source for ectomycorrhizal growth.

• Journal of Plant Biology
• /
• 제12권2호
• /
• pp.1-6
• /
• 1969

Effects of the special media on the mycelium growth in Agaricus campestris were studied. The results might be summarized as follows: 1. The mycelium growth fo Agaricus campestris were scarecely stimulated on the Peptone basal medium which was added 0.5gr. of Peptone and Dextrose basal medium which was added 1.5gr. of dextrose during the culture for 144 hours. 2. The mycelium of Agaricus campestris on the media which was added the several kinds of vegetable extracts showed a considerable growth for 144 hours. The order is as follows; Carrot-basal medium(4ml./100ml.)>Tomato-basal medium(2ml./100ml.)>Spinach-basal medium(3ml./100ml.). However, the spinach-basal medium among these three media were no significant difference.

• BMB Reports
• /
• 제49권10호
• /
• pp.566-571
• /
• 2016

Forskolin (FSK), an adenylyl cyclase activator, has recently been shown to enhance nucleotide excision repair (NER) upon UV exposure. However, our study revealed that this effect was detected in human skin epithelial ARPE19 cells only in growing cells, but not in non-cycling cells. When the cells were grown at low density (70% confluence), FSK was capable of stimulating cAMP responsive element binding (CREB) phosphorylation, a marker for FSK-stimulated PKA activation, and resulted in a significant increase of NER activity compared to control treatment. However, cells grown under 100% confluent conditions showed neither FSK-induced CREB phosphorylation nor the resulting NER enhancement. These findings indicate that cellular growth is critical for FSK-induced NER enhancement and suggest that cellular growth conditions should be considered as a variable while evaluating a reagent's pharmacotherapeutic efficacy.

• Mycobiology
• /
• 제30권2호
• /
• pp.70-75
• /
• 2002

The mycelia were directly isolated from eight species of fungal basidiocarps, confirmed to the ectomycorrhiza in the roots from the fields(forestry); Suillus bovinus, Paxillus involutus, Lactarius hysginus, Russula fragilis, Lepista nuda, Lyophyllum shimeji, Tricholoma matsutake, and Russula integra. The mycelia were pure-cultured with several transferring in various agars, and inoculated to the roots of pine(Pinus densiflora) seedling by in vitro method. After ten months growth under artificially aseptic conditions, all pine seedlings inoculated were stimulated at the growth-height, whereas those not inoculated were nearly dead. Also, the ramifications of ectomycorrhizal pine roots formed in the synthetic in vitro systems and were various according to the different mycelia. Synthesis of ectomycorrhiza were clearly confirmed in ten months growth, but not distinguished at this moment. It was clearly proved that the mycelia isolated caused the ectomycorrhizae in the roots of pine seedlings.

• KSBB Journal
• /
• 제8권1호
• /
• pp.55-61
• /
• 1993

Ginkgo biloba의 세포배양을 통하여 세포성장과 flavonol glycosides의 생성에 영향을 미치는 여러 조건들을 연구하였다. 다양한 지역으로부터 유도된 세포주들의 세포성장과 flavonol glycosides의 생합성 능력은 서로 차이가 있었다. 이들의 캘러스 및 현탁 세포는 배지조건과 배양조건에 따라 성장 및 f1avonol glycosides 생합성에 많은 영향을 받았었다. 특히 배양조건 중 빛의 영향은 현저하여 명조건에서의 f1avonol glycosides의 생성은 암조건보다 10배 이상 증가함을 알 수 있었다.

• 미생물학회지
• /
• 제25권3호
• /
• pp.189-197
• /
• 1987

배지성분으로 첨가된 glutamate의 농도가 균의성장고 tylosin 생합성에 미치는 영향을 조사하였다. 그 결과 oxaloacetate를 공동기질로 사용하는 효소의 활성에 의하여 균의 성장과 tylosin의 생합성이 조절됨을 알았다. 즉, citrate synthase와 aspartate aminotransferase의 활성은 균의 성장에 아주 긴요하며 methylmalonyl-CoA carboxytransferase의 활성은 tylosin 생합성에 아주 중요한 효소임을 알았다. Glutamate의 농도는 우의 효소의 활성에 직접적으로 영향을 주고 있음을 알았다.