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Cytotoxicological Effect of Tebufenozide, an Insect Growth Regulator (IGR): Stimulation of Filamentous Actin Reorganization and Enhancement of Hsp27 Expression in Drosophila Kc Cells  

Hwang, Jee-Na (Department of Pharmacology, Medical Research Center (MRC), Kyung Hee University School of Medicine)
Jung, Hwa-Jin (Department of Pharmacology, Medical Research Center (MRC), Kyung Hee University School of Medicine)
Seo, Young-Rok (Department of Pharmacology, Medical Research Center (MRC), Kyung Hee University School of Medicine)
Publication Information
The Korean Journal of Physiology and Pharmacology / v.8, no.6, 2004 , pp. 351-354 More about this Journal
Abstract
The cytotoxicological responses to insect growth regulator (IGR), using tebufenozide as ecdysteroid mimic, were investigated in Drosophila Kc cells. Treatment of Kc cells with tebufenozide showed significant growth inhibition and striking morphological changes including aggregation and elongation of the cells. In order to understand the cellular mechanism underlying the response of Drosophila cells to tebufenozide, immunofluorescence microscopy was performed. We found that treatment of Kc cells with tebufenozide enhanced the reorganization of f-actin and stimulated the expression of hsp27. These data suggest a possible association of filamentous actin (f-actin) and hsp27 in the cytotoxicological mechanisms of growth regulators in Drosophila cells.
Keywords
IGR; Tebufenozide; Drosophila; f-actin; hsp27;
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1 Cherbas L, Bene H, Bourouis M, Burtis K, Chao A, Cherbas P, Crosby M, Garfinkel M, Guild G, Hogness D, Jami J, Jones CW, Koehler M, Lepesant JA, Martin C, Maschat F, Mathers P, Meyerowitz E, Moss R, Pictet R, Rebers J, Richards G, Roux J, Schulz R, Segraves W, Thummel C, Vijayraghavan K. Structural and functional analysis of some moulting hormone-responsive genes from Drosophila. Insect Biochem 16: 241－248, 1986   DOI   ScienceOn
2 Echalier G, Ohanessian A. Isolation in tissue culture, of Drosophila melangaster cell lines. C R Acad Sci Hebd Seances Acad Sci D. 268: 1771-1773, 1969
3 Li GC, Li L, Liu YK, Mak JY, Lee C. Thermal response of rat fibroblasts stably transfected with the human 70-kDa heat shock protein-encoding genes. Proc Natl Acad Sci USA 88: 1681－1685, 1991   DOI   ScienceOn
4 Ritossa F. A New puffing pattern induced by temperature shock and DNP in Drosophila. Experientia 18: 571－573, 1962   DOI   ScienceOn
5 Chen GC, Gajowniczek P, Settleman J. Rho-LIM kinase signaling regulates ecdysone-induced gene expression and morphogenesis during Drosophila metamorphosis. Curr Biol 14: 309－313, 2004   DOI
6 de Jong WW, Leunissen JA, Voorter CE. Evolution of the alphacrystallin/ small heat-shock protein family. Mol Biol Evol 10: 103 －126, 1993   PUBMED
7 Riddiford LM. Atlas of Drospheila Development: Drosophila Third Instar Eye Disk Chart. In: Bate M, Arias AM ed, The development of Drosophila melanogaster. Cold Spring Horbor Lab Press, Plainview, NY, p 899－939, 1993
8 Courgeon AM. Action of insect hormones at the cellular level: Morphological changes of a diploid cell line of Drosophila melanogaster treated with ecdysone and several analogs in vitro. Exp Cell Res 74: 327-336, 1972   DOI   ScienceOn
9 Anne JM, Martin SJ, Bissonnette RP, Mahboubi A, Shi Y, Mogil RJ, Nishioka WK, Green DR. Cell Death. In: Schwartz L, Osborne B ed, Methods in Cell Biology. Academic Press, San Diego, CA, p 153－185, 1995
10 Geneviĕve M, Yutaka I, Kanefusa K, Robert MT. The small heat shock protein Hsp22 of Drosophila melanogaster is a mitochondrial protein displaying oligomeric organization. J Biol Chem 275: 31204－31210, 2000   DOI   ScienceOn
11 Ropp M, Courgeon AM, Calvayrac R, Best-Belpomme M. The possible role of the superoxide ion in the induction of heat-shock and specific proteins in aerobic Drosophila cells during return to normoxia after a period of anaerobiosis. Can J Biochem Cell Biol 61: 456－461, 1983   DOI
12 Chen L, Reece C, O'Keefe SL, Hawryluk GW, Engstrom MM, Hodgetts RB. Induction of the early-late Ddc gene during Drosophila metamorphosis by the ecdysone receptor. Mech Dev 114: 95－107, 2002   DOI   PUBMED   ScienceOn
13 Nakagawa Y, Minakuchi C, Takahashi K, Ueno T. Inhibition of $[^3H]$ponasterone A binding by ecdysone agonists in the intact Kc cell line. Insect Biochem and Mol Biol 32: 175－180, 2002   DOI   ScienceOn
14 Mehlen P, Kretz-Remy C, Preville X, Arrigo AP. Human hsp27, Drosophila hsp27 and human alphaB-crystallin expressionmediated increase in glutathione is essential for the protective activity of these proteins against TNF alpha-induced cell death. EMBO J 15: 2695－2706, 1996   PUBMED
15 Cherbas P, Cherbas L, Williams CM. Induction of acetylcholinesterase activity by -ecdysone in a Drosophila cell line. Science 197: 275-277, 1977   DOI   ScienceOn
16 Marks EP. Insect tissue culture: An overview. Annu Rev Entomol 73: 1971－1978, 1980
17 Cherbas L, Fristrom JW and O'connor JD. Biosynthesis, Metabolism and Mode of Action of Invertebrate Hormones. In: Hoffmann J, Porchet M ed, Proceedings in Life Sciences. Springer- Verlag, Berlin, p 305-322, 1984
18 Wang HD, Kazemi-Esfarjani P, Benzer S. Multiple-stress analysis for isolation of Drosophila longevity genes. PNAS 101: 12610－ 12615, 2004   DOI   ScienceOn
19 Hock T, Cottrill T, Keegan J, Garza D. The E23 early gene of Drosophila encodes an ecdysone-inducible APT-binding cassette transporter capable of repressing ecdysone-mediated gene activation. PNAS 97: 9519－9524, 2000   DOI   ScienceOn
20 Best-Belpomme M, Courgeon AM, Rambach A. Galactosidase is induced by hormone in Drosophila melanogaster cell cultures. Proc Natl Acad Sci USA 75: 6102－6106, 1978   DOI   ScienceOn