• 원예과학기술지
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• 제29권4호
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• pp.374-381
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• 2011

Stilbene 화합물은 항산화 및 항균 활성을 가지는 폴리페놀계의 천연물이다. 포도를 포함하는 몇몇 종류의 식물에서 stilbene 화합물은 resveratrol의 유도체로서 매우 다양한 형태로 존재한다. 자외선 조사 포도 잎에서 stibene 화합물을 정량 분석하기에 앞서, 먼저 각 stilbene 화합물을 동정하기 위한 실험을 수행하였다. 이를 위하여, 자외선을 심하게 조사한 잎에서 stilbene 배당체를 추출하였다. 추출한 배당체 시료는 ${\beta}$-glucosidase를 이용하여 가수분해한 후, 특정 stilbene 화합물의 질량에 해당하는 m/z에서 HPLC-mass spectrometer를 이용하여 분석하였다. 효소적 가수분해에 의해 chromatogram상에는 glycoside에 해당할 것으로 예상되는 peak의 감소와 aglycone에 해당할 것으로 예상되는 peak 증가가 나타났다. 또한 stilbene 화합물의 광 이성질화를 유도하기 위하여 시료를 일광에 노출하였으며, 광 노출에 의해 trans-isomer에 해당할 것으로 예상되는 peak의 소멸과 cis-isomer에 해당할 것으로 예상되는 peak의 생성이 나타났다. Chromatogram상의 peak의 이러한 증감으로부터 각 peak의 성분을 유추하였다. 이러한 방법으로 포도 잎에서 16종의 stilbene 화합물을 동정할 수 있었으며 자외선을 조사한 포도 잎에서 동정된 화합물에 대한 정량적 분석을 수행하였다. 자외선 조사는 포도 잎에서 총 stilbene 함량의 상당한 증가를 가져왔는데 특히 trans-resveratrol은 수백 배 증가하였다. 또한, resveratrol보다 더 강한 radical 소거 활성을 가지지만 무처리 잎에서는 단지 미량으로만 존재하는 piceatannol의 함량 역시 수십배 증가하였다. 자외선 조사에 의한 이러한 stilbene 함량의 증가는 hormesis 현상으로서 포도의 스트레스 대응 반응의 하나로 생각된다.

• 약학회지
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• 제43권2호
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• pp.233-236
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• 1999

In order to clarify the anti-Ohyul activity of rhubarb, we investigated the effects of water extract from rhizomes of four different rhubarb on blood platelet aggregation induced by arachidonic acid, ADP, collagen and PAF in vitro. The cultivated Korean rhubarb rhizomes (Rheum undulatum) exhibited the most potent inhibitory action on the aggregation induced by arachidonic acid and also among the four fractions, stilbene components containing part showed strong inhibitory action. These inhibitory effect may partially contributed to anti-Ohyul activity of rhubarb.

• 생약학회지
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• 제13권4호
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• pp.145-152
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• 1982

Three species of genus Polygonum, namely P. cuspidatum, P. sachalinense and P. ellipticum are distributed in Korea. Polygonum ellipticum Migo is a perennial herb in Polygonaceous plants. The root of the plant (Polygoni Rhizoma, 'Ho-jang') have been used as laxative, diuretic and for the treatment suppurative dermatitis in the oriental medicine. As the part of the study for the comparison of the three species in their components, the authors attempted to isolate the anthraquinones and stilbene derivative from the rhizome of P. ellipticum. The methanolic extract of dried rhizome of this plant was fractionated into ether soluble and insoluble fraction and each fraction was applied to column chromatography to isolate above mentioned components. Anthraquinone derivatives were isolated first; comp. I, mp $204{\sim}205^{\circ}$ (physcion), comp. II, mp $254{\sim}255^{\circ}$ (emodin), comp. IV, mp $191{\sim}192^{\circ}$ $(emodin-8-O-{\beta}-D-glucoside)$ and comp. III, mp $280{\sim}282^{\circ}$ $({\beta}-sitosterol-glucoside)$. They were identified by chemical properties and UV, IR and NMR spectra and by the direct comparison with authentic samples. Stilbene derivative was isolated secondly; comp. V, mp $255{\sim}256^{\circ}$ which was reported to possess antibacterial and antifungal activities.

• Natural Product Sciences
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• 제17권2호
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• pp.65-79
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• 2011

Reactive oxygen species potentially cause damage to cellular components including lipids, protein and DNA; this oxidative damage plays a key role in the pathogenesis of neurodegenerative disease, cardiovascular disease, metabolic disease and cancer. On the basis of the oxidative stress hypothesis, a number of studies have been performed to search for an efficient and safe antioxidant. Although in vitro studies have provided promising results, only a limited number of natural and synthetic antioxidants have been developed for clinical application due to their low efficacy and side-effects. Thus, the discovery of new antioxidants with marked efficacy and safety has attracted worldwide attention in recent decades. Since plants are recognized as important sources of natural antioxidants, our research has focused on the discovery of new naturally occurring antioxidants from medicinal plants. The purpose of this review is to open a new prospect in the field of search for natural antioxidants from medicinal plants by summarizing our recent findings. Using in vitro bioassay systems such as 2,2-diphenyl-1-picrylhydrazyl, superoxide radical scavenging tests and lipid peroxidation models, we have tested over than 350 species of medicinal plants for their antioxidant activity and selected several of them for further investigation. During the research on the discovery of effective natural antioxidants from the medicinal plants selected, we have isolated several new and known antioxidant compounds that include stilbene glycosides, phenolic glycosides, flavonoids, oligostilbenes, and coumarins. Our results suggest that the presence of antioxidant compounds in the medicinal plants might be associated with the traditional use to treat inflammation, cardiovascular disease and various chronic diseases.

• 대한약침학회지
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• 제27권2호
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• pp.70-81
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• 2024

Objectives: Cognitive impairments, ranging from mild to severe, adversely affect daily functioning, quality of life, and work capacity. Despite significant efforts in the past decade, more than 200 promising drug candidates have failed in clinical trials. Herbal remedies are gaining interest as potential treatments for dementia due to their long history and safety, making them valuable for drug development. This review aimed to examine the mechanisms behind the effect of Polygonum multiflorum on cognitive function. Methods: This study focused primarily on the effects of Polygonum multiflorum and its chemical constituents on cognitive behavioral outcomes including the Morris water maze, the passive avoidance test, and the Y maze, as well as pathogenic targets of cognitive impairment and Alzheimer's disease (AD) like amyloid deposition, amyloid precursor protein, tau hyperphosphorylation, and cognitive decline. Additionally, a thorough evaluation of the mechanisms behind Polygonum multiflorum's impact on cognitive function was conducted. We reviewed the most recent data from preclinical research done on experimental models, particularly looking at Polygonum multiflorum's effects on cognitive decline and AD. Results: According to recent research, Poligonum multiflorum and its bioactive components, stilbene, and emodin, influence cognitive behavioral results and regulate the pathological target of cognitive impairment and AD. Their mechanisms of action include reducing oxidative and mitochondrial damage, regulating neuroinflammation, halting apoptosis, and promoting increased neurogenesis and synaptogenesis. Conclusion: This review serves as a comprehensive compilation of current experiments on AD and other cognitive impairment models related to the therapeutic effects of Polygonum multiflorum. We believe that these findings can serve as a basis for future clinical trials and have potential applications in the treatment of human neurological disorders.

• Archives of Pharmacal Research
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• 제17권1호
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• pp.42-44
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• 1994

The activity-guided fractionation of some medicinal plants led to yield five kinds of natural stilbene compounds namely 3, 5-dihydroxy-4'-methoxystillbene(I), rhapontigenin(II), reveratrol(III), rhaponticin(IV) and piceid(V) and two common flavonoids, apigenin(VI) and luteolin(VII) as active principles of the antitumor property, in vitro, against five kinds of human tumor cell lines, A-549, SK-OV-3, SK-MEL-2, XF-498 and HCT15.

• Natural Product Sciences
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• 제20권2호
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• pp.91-94
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• 2014

Column chromatographic separation of the MeOH extract from the tubers of Bletilla striata yielded seven phenolic components including four phenanthrenes, 3,7-dihydroxy-2,4-dimethoxyphenanthrene (1), 3,7-dihydroxy-2,4,8-trimethoxyphenanthrene (2), 9,10-dihydro-4,7-dimethoxyphenanthrene-2,8-diol (3), and 9,10-dihydro-1-(4'-hydroxybenzyl)-4,7-dimethoxyphenanthrene-2,8-diol (4) and three stilbenes, gigantol (5), 3',4"-dihydroxy-5',3",5"-trimethoxybibenzyl (6), and batatasin III (7). Their structures were determined on the basis of NMR spectroscopic data. Among them, compound 2, 3, and 6 were reported for the first time from this plant. The isolated compounds (1-7) were tested for cytotoxicity against four human tumor cell lines in vitro using a Sulforhodamin B bioassay.

• 임산에너지
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• 제19권2호
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• pp.86-92
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• 2000

α-Cellulose를 페놀 및 황산과 1: 6.2 : 0.05(g/g㎖)의 비율로 혼합하여 질소기류 하에서 180℃에서 60분의 액화 처리한 후 변호된 셀룰로오스의 성분분석을 GC-MS에 의하여 실시한 결과는 다음과 같다. 셀룰로오스의 액화율이 98.8%로 높은 것으로 보아 상당히 저분자화 된 것을 알 수 있었다. 셀룰로오스 액화물 중의 13.6%인 페놀함유량은 용매로 투입된 페놀의 양보다 매우 적은데 이는 액화 생성된 화합물의 구조 중에 hydroxyphenly기나 phenyl기가 존재하는 것에서 셀룰로오스는 액화시 산 촉매에 의하여 C-C, C-O 결합의 일부 개열함과 동시에 페놀과 반응하기 때문이라 판단된다. 약 54%인 12개 셀룰로오스 액화물의 구조를 분석하였으며, 그 중 양적으로 많은 양이 검출된 물질은 phenol핵이 주종을 이루는 2,2\`-methylenebisphenol, 4,4\`-methylene-bisphenol, 3-methyl-4-hydroxyphenyl-2\`-hydroxyphenylmethane, 1-methoxy-4-(2-phenylethenyl) benzene, (e)-2,4\` dihydroxystilbene, 1-phenyl-1-(4\`-hydroxy) phenyl methanol, p-isopropylphenol 등이 검출되었다. 황산 촉매에 의하여 셀룰로오스는 액화과정에서 탈수반응 후 열분해 되어 셀룰로오스링이 끊어져 생성된 중간체들이 용매인 phenol과 치넌자 치환반응을 일으켜 phenol 화합물들을 많이 생성한다고 판단된다.

• Journal of Nutrition and Health
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• 제53권6호
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• pp.570-582
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• 2020

본 연구는 상지추출물과 stilbene 화합물인 MSA, ORT의 항비만 효능을 비교 분석하고자 식이성 비만 마우스 모델을 이용하여 HFD에 실험물질을 첨가한 식이를 13주 동안 급여하였다. 연구결과, 실험물질들의 항비만 효능은 ORT, ME, MSA, MW군 순이었으며, 특히 ME와 ORT의 급여는 고지방식이에 의한 체중 및 체지방 증가, 혈당 증가, 이상지질혈증 및 지방간 개선에 있어 양성대조군인 GC군과 비슷한 효과를 보였다. 향후 관련 기작 규명 및 안전성 확보, 그리고 최대 유효성 도출을 위한 추출 조건 확립 및 용량 검증 등의 연구가 수행된다면 체중조절용 기능성 식품으로의 개발을 기대해 볼 수 있을 것으로 사료된다.