Journal of Nutrition and Health

The Korean Nutrition Society (한국영양학회)
권호 표지
DOI QR코드

DOI QR Code

Anti-obesity effect of Ramulus mori extracts and stilbenes in high fat dietfed C57BL/6J mouse

고지방식이를 급여한 C57BL/6J 마우스에서 상지추출물과 스틸벤 화합물의 항비만 효능 연구

  • Park, Jeong-Eun (Department of Food Science and Nutrition, Daegu Catholic University) ;
  • Lee, Geon-Hee (Department of Food Science and Nutrition, Daegu Catholic University) ;
  • Kim, Juhee (Department of Food Science and Nutrition, Daegu Catholic University) ;
  • Choi, Sang-Won (Department of Food Science and Nutrition, Daegu Catholic University) ;
  • Kim, Eunjung (Department of Food Science and Nutrition, Daegu Catholic University)
  • 박정은 (대구가톨릭대학교 식품영양학과) ;
  • 이건희 (대구가톨릭대학교 식품영양학과) ;
  • 김주희 (대구가톨릭대학교 식품영양학과) ;
  • 최상원 (대구가톨릭대학교 식품영양학과) ;
  • 김은정 (대구가톨릭대학교 식품영양학과)
  • Received : 2020.10.14
  • Accepted : 2020.11.12
  • Published : 2020.12.31
Download PDF
⟨ Previous Next ⟩

Abstract

Purpose: Obesity is a risk factor for various adult diseases such as type 2 diabetes, cardio-cerebrovascular disease, and cancer. With an increasing obesity population worldwide, the prevention of obesity with natural components has emerged as an alternative health care strategy. Ramulus mori (Sangzhi, RM) is widely used as a traditional herbal medicine in East Asia. It contains various phytochemicals, including stilbenes and 2-arylbenzofurans. In this study, we compared the anti-obesity effects of RM extracts and its major stilbene components (mulberroside A [MSA] and oxyresveratrol [ORT]) in high fat diet (HFD)-fed obese mice. Methods: Five week-old, male C57BL/6J mice were grouped into 7 experimental groups: normal diet (ND), HFD, HFD + 1% RM water extracts (MW), HFD + 0.1% MSA, HFD + 1% RM ethanol extracts (ME), HFD + 0.1% ORT, and HFD + 1% Garcinia cambogia extracts (GC) as a positive control. All mice were fed experimental diet for 13 weeks. Results: Compared to the HFD group, total body weight and weekly body weight gain were significantly decreased in the ME, ORT, and GC groups. Glucose tolerance level was significantly decreased in all experimental groups, whereas plasma insulin level was decreased in MSA, ME, ORT and GC groups. Plasma glucose, triglyceride (TG), and total cholesterol levels were significantly decreased, whereas high-density lipoprotein cholesterol levels were increased in the MSA, ORT, and GC groups. Hepatic TG accumulation was also significantly decreased in the MSA, ME, ORT, and GC groups. Adipose tissue weight and size of adipocytes were significantly decreased in the MSA, ME and ORT groups, and were comparable to values obtained in the GC group. The levels of adiponectin and SREBP1c mRNA expressions were increased in the ORT and GC groups. Conclusion: These results indicate that ME, ORT and MSA exert significant anti-obesity effect, and have the potential to be developed as a weight control ingredient of functional foods.

본 연구는 상지추출물과 stilbene 화합물인 MSA, ORT의 항비만 효능을 비교 분석하고자 식이성 비만 마우스 모델을 이용하여 HFD에 실험물질을 첨가한 식이를 13주 동안 급여하였다. 연구결과, 실험물질들의 항비만 효능은 ORT, ME, MSA, MW군 순이었으며, 특히 ME와 ORT의 급여는 고지방식이에 의한 체중 및 체지방 증가, 혈당 증가, 이상지질혈증 및 지방간 개선에 있어 양성대조군인 GC군과 비슷한 효과를 보였다. 향후 관련 기작 규명 및 안전성 확보, 그리고 최대 유효성 도출을 위한 추출 조건 확립 및 용량 검증 등의 연구가 수행된다면 체중조절용 기능성 식품으로의 개발을 기대해 볼 수 있을 것으로 사료된다.

Keywords

Acknowledgement

This work was supported by research grants from Dongil Culture and Scholarship Foundation in 2019

References

  1. Ministry of Health and Welfare, Korea Centers for Disease Control and Prevention. Korea Health Statistics 2018: Korea National Health and Nutrition Examination Survey (KNHANES VII-3). Cheongju: Korea Centers for Disease Control and Prevention; 2019.
  2. National Health Insurance Service. A study on the improvement of weight reduction programme for insurer to enhence life expectancy. Wonju: National Health Insurance Service; 2017.
  3. Commercializations Promotion Agency for R&D Outcomes. Market trends in the metabolic syndrome therapeutics. Seoul: Commercializations Promotion Agency for R&D Outcomes; 2015.
  4. Publishing Committee of Pharmacognosy. Pharmacognosy. Seoul: Dongmyongsa; 2000. p.257.
  5. Choi SW, Jang YJ, Lee YJ, Leem HH, Kim EO. Analysis of functional constituents in mulberry (Morusalba L.) twigs by different cultivars, producing areas, and heat processings. Prev Nutr Food Sci 2013; 18(4): 256-262. https://doi.org/10.3746/pnf.2013.18.4.256
  6. Chen YC, Tien YJ, Chen CH, Beltran FN, Amor EC, Wang RJ, et al. Morus alba and active compound oxyresveratrol exert anti-inflammatory activity via inhibition of leukocyte migration involving MEK/ERK signaling. BMC Complement Altern Med 2013; 13(1): 45. https://doi.org/10.1186/1472-6882-13-45
  7. Zheng ZP, Cheng KW, Zhu Q, Wang XC, Lin ZX, Wang M. Tyrosinase inhibitory constituents from the roots of Morus nigra: a structure-activity relationship study. J Agric Food Chem 2010; 58(9): 5368-5373. https://doi.org/10.1021/jf1003607
  8. Lorenz P, Roychowdhury S, Engelmann M, Wolf G, Horn TF. Oxyresveratrol and resveratrol are potent antioxidants and free radical scavengers: effect on nitrosative and oxidative stress derived from microglial cells. Nitric Oxide 2003; 9(2): 64-76. https://doi.org/10.1016/j.niox.2003.09.005
  9. Tan HY, Tse IM, Li ET, Wang M. Inhibitory effects of oxyresveratrol and cyanomaclurin on adipogenesis of 3T3-L1 cells. J Funct Foods 2015; 15: 207-216. https://doi.org/10.1016/j.jff.2015.03.026
  10. Lee W, Ham J, Kwon HC, Yoon G, Bae GU, Kim YK, et al. Amorphastilbol exerts beneficial effects on glucose and lipid metabolism in mice consuming a high-fat-diet. Int J Mol Med 2015; 36(2): 527-533. https://doi.org/10.3892/ijmm.2015.2227
  11. Tan HY, Tse IM, Li ET, Wang M. Oxyresveratrol supplementation to C57bl/6 mice fed with a high-fat diet ameliorates obesity-associated symptoms. Nutrients 2017; 9(2): E147.
  12. Lee JH, Baek SY, Jang EJ, Ku SK, Kim KM, Ki SH, et al. Oxyresveratrol ameliorates nonalcoholic fatty liver disease by regulating hepatic lipogenesis and fatty acid oxidation through liver kinase B1 and AMPactivated protein kinase. Chem Biol Interact 2018; 289: 68-74. https://doi.org/10.1016/j.cbi.2018.04.023
  13. Folch J, Lees M, Sloane Stanley GH. A simple method for the isolation and purification of total lipides from animal tissues. J Biol Chem 1957; 226(1): 497-509. https://doi.org/10.1016/S0021-9258(18)64849-5
  14. Osman OS, Selway JL, Kepczynska MA, Stocker CJ, O'Dowd JF, Cawthorne MA, et al. A novel automated image analysis method for accurate adipocyte quantification. Adipocyte 2013; 2(3): 160-164. https://doi.org/10.4161/adip.24652
  15. Deng H, He X, Xu Y, Hu X. Oxyresveratrol from mulberry as a dihydrate. Acta Crystallogr Sect E Struct Rep Online 2012; 68(Pt 5): o1318-o1319. https://doi.org/10.1107/S1600536812014018
  16. Galindo I, Hernaez B, Berna J, Fenoll J, Cenis JL, Escribano JM, et al. Comparative inhibitory activity of the stilbenes resveratrol and oxyresveratrol on African swine fever virus replication. Antiviral Res 2011; 91(1): 57-63. https://doi.org/10.1016/j.antiviral.2011.04.013
  17. Chung KO, Kim BY, Lee MH, Kim YR, Chung HY, Park JH, et al. In-vitro and in-vivo anti-inflammatory effect of oxyresveratrol from Morus alba L. J Pharm Pharmacol 2003; 55(12): 1695-1700. https://doi.org/10.1211/0022357022313
  18. Aftab N, Likhitwitayawuid K, Vieira A. Comparative antioxidant activities and synergism of resveratrol and oxyresveratrol. Nat Prod Res 2010; 24(18): 1726-1733. https://doi.org/10.1080/14786410902990797
  19. Park SY, Jin B, Shin JH, Adisakwattana S, Kwon O. Standardized Mori ramulus extract improves insulin secretion and insulin sensitivity in C57BLKS/J db/db mice and INS-1 cells. Biomed Pharmacother 2017; 92: 308-315. https://doi.org/10.1016/j.biopha.2017.05.080
  20. Ahn E, Lee J, Jeon YH, Choi SW, Kim E. Anti-diabetic effects of mulberry (Morus alba L.) branches and oxyresveratrol in streptozotocin-induced diabetic mice. Food Sci Biotechnol 2017; 26(6): 1693-1702. https://doi.org/10.1007/s10068-017-0223-y
  21. Park KT, Kim JK, Hwang D, Yoo Y, Lim YH. Inhibitory effect of mulberroside A and its derivatives on melanogenesis induced by ultraviolet B irradiation. Food Chem Toxicol 2011; 49(12): 3038-3045. https://doi.org/10.1016/j.fct.2011.09.008
  22. Zheng ZP, Tan HY, Wang M. Tyrosinase inhibition constituents from the roots of Morus australis. Fitoterapia 2012; 83(6): 1008-1013. https://doi.org/10.1016/j.fitote.2012.06.001
  23. Chao J, Yu MS, Ho YS, Wang M, Chang RC. Dietary oxyresveratrol prevents parkinsonian mimetic 6-hydroxydopamine neurotoxicity. Free Radic Biol Med 2008; 45(7): 1019-1026. https://doi.org/10.1016/j.freeradbiomed.2008.07.002
  24. Andrabi SA, Spina MG, Lorenz P, Ebmeyer U, Wolf G, Horn TF. Oxyresveratrol (trans-2,3′,4,5′-tetrahydroxystilbene) is neuroprotective and inhibits the apoptotic cell death in transient cerebral ischemia. Brain Res 2004; 1017(1-2): 98-107. https://doi.org/10.1016/j.brainres.2004.05.038
  25. Kim KY, Lee HN, Kim YJ, Park T. Garcinia cambogia extract ameliorates visceral adiposity in C57BL/6J mice fed on a high-fat diet. Biosci Biotechnol Biochem 2008; 72(7): 1772-1780. https://doi.org/10.1271/bbb.80072
  26. Barnea M, Shamay A, Stark AH, Madar Z. A high-fat diet has a tissue-specific effect on adiponectin and related enzyme expression. Obesity (Silver Spring) 2006; 14(12): 2145-2153. https://doi.org/10.1038/oby.2006.251

Cited by

  1. Oxyresveratrol: Sources, Productions, Biological Activities, Pharmacokinetics, and Delivery Systems vol.26, pp.14, 2020, https://doi.org/10.3390/molecules26144212