• 생약학회지
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• 제13권3호
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• pp.129-131
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• 1982

From the roots of Codonopsis pilosula (Franch.) Nannfeldt (Campanulaceae), ${\alpha}$-spinasterol, mp $165{\sim}7^{\circ}$ and alpha-spinasteryl D-glucoside, mp $283{\sim}5^{\circ}$, were isolated and identified on the basis of chemical and spectral data. Spectral data showed that $\Delta^7-stigmasterol$ and its glucoside were contained as minor components.

• 약학회지
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• 제18권3호
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• pp.203-208
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• 1974

An acylated steryl glucoside preparation has been isolated from Phytolacca esculenta $_{VAN}H_{OUTTE}$. Two componentsof the vsterol moiety are ${\alpha}$-spinasterol and ${\delta}^7$-stigmastenol. Fatty acids are palmitic, stearic and myristic acids, and are located on C-6 of the glucose portion. 6'-Palmityl-${\alpha}$-spinasteryl-${\beta}$-glucoside is the main component.

• Journal of Microbiology and Biotechnology
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• 제26권3호
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• pp.477-482
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• 2016

A uridine diphosphate-glucose:sterol glycosyltransferase-encoding gene was isolated and cloned from the established fosmid library of Micromonospora rhodorangea ATCC 27932 that usually produces the aminoglycoside antibiotic geneticin. The gene consists of 1,185 base pairs and encodes a 41.4 kDa protein, which was heterologously expressed in Escherichia coli BL21(DE3). In silico analyses of the deduced gene product suggested that it is a member of the family 1 glycosyltransferases. The recombinant protein MrSGT was able to catalyze the transfer of a glucosyl moiety onto the C-3 hydroxy function in sterols (β-sitosterol, campesterol, and cholesterol), resulting in the corresponding steryl glucosides (β-sitosterol-3-O-β-ᴅ-glucoside, campesterol-3-O-β-ᴅ-glucoside, and cholesterol-3-O-β-ᴅ-glucoside). This enzyme prefers phytosterols to cholesterol, and also shows substrate flexibility to some extent, in that it could recognize a number of acceptor substrates.

• Journal of Ginseng Research
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• 제9권2호
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• pp.193-203
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• 1985

Lipid and fatty acid compositions of the free lipids in Panax ginseng (Korea, Japan and China), Panax quinquefolium (America, Canada) and Panax notoginseng (China) were studied by means of silicic acid column chromatography, thin-layer chromatography and gas-liquid chromatography. Free lipid contents were 1.13 to 1.24% in panax ginseng and 0.87 to 1.18% in Panax quinquefolium and 0.39% in panax notoginseng. Neutral lipid fractions were 81.2 to 84.4%, while glycolipid fractions 8.01% to 14.47% and phospholipid fractions 3.49 to 5.74% in free lipid contents. The major components were triglycerides, free sterols, diglyceride, free fatty acids and sterol esters in neutral lipid fractions, sterol glucoside, monogalactosyl diglyceride, digalactosyl diglyceride, esterified steryl glucoside in glycolipid fractions and phosphatidyl glycerol, phosphatide, ethanolamine, phosphatidyl choline in phospholipid fractions. The contents and compositions of neutral lipids and glyclipids were some different among various ginsengs, whereas phospholipids showed relatively similar compositions in the contents. Seventeen fatty acids were analyzed in the four free lipid fractions from the various ginsengs and the main fatty acids were linoleic acid, palmitic acid, oleic acid and linolenic acid. It was found that the amounts of some fatty acids were different among the various ginsengs, but the fatty acid patterns of these ginsengs were on the whole similar.

• 생약학회지
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• 제21권1호
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• pp.56-59
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• 1990

n-Alkanes (n-nonacosane and n-hentriacontane), phytosterols (campesterol and ${\beta}$-sitosterol) and phytosteryl glucosides (${\beta}$-sitosterol 3-O-${\beta}$-D-glucopyranoside and campesterol 3-O-${\beta}$-D-glucopyranoside) were isolated from the underground parts of Epimedium koreanum (Berberidaceae) and characterized by spectral data.

• 생약학회지
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• 제22권2호
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• pp.78-84
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• 1991

From the whole plants of Orostachys japonicus(Crassulaceae), fatty acid ester mixture, seco-A-triterpene mixture, glutinone, friedelin, ${\beta}-amyrin$, glutinol, epifridelanol, 1-hexatriacontanol, sterol mixture, steryl glucoside mixture were isolated and characterized by spectral data.

• 생약학회지
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• 제16권3호
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• pp.141-150
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• 1985

Lipid and fatty acid compositions of free lipids and bound lipids from fresh ginseng, red ginseng and white ginseng were studied by means of silicic acid column chromatography, thin-layer chromatography and gas-liquid chromatography. Free lipid and bound lipid contents in those three samples were 1.21 to 1.45% and 0.32 to 0.45%. Neutral lipid fractions in free lipids from the samples were 76.6 to 79.7%, while glycolipid and phospholipid fractions were 11.6 to 14.7% and 8.5 to 8.7%, respectively. The major lipids were triglycerides, sterol esters and hydrocarbons, diglycerides and free sterols in neutral lipids, sterol glucoside, monogalactosyl diglyceride, esterified steryl glycoside, digalactosyl diglyceride in glycolipids and phosphatidyl ethanolamine, phosphatidyl glycerol, phosphatidyl choline and phosphatidyl inositol in phospholipids. Fourteen kinds of even numbered and four kinds of odd numbered fatty acids were identified in the four lipid fractions (TL, NL, GL and PL) by GLC, and the main fatty acids were linoleic acid, palmitic acid, oleic acid and linolenic acid.

• Journal of Ginseng Research
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• 제9권2호
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• pp.204-212
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• 1985

Lipid and fatty acid compositions of the bound lipids in Panax quinquefolium (Korea, Japan and China), Panax quinquefolium (America, Canada) and Panax notoginseng (China) were studied by means of silicic acid column chromatography, thin-layer chromatography and gas-liquid chromatography. The could lipid contents in various ginsengs were 0.29 to 0.48%, in which neutral lipid fractions were 63.6 to 67.3%, glycolipid fractions 21.9 to 25.7% and phospholipid fractions 7.7 to 12.4%. The content compositions of neutral lipid fractions were lower and those of glycolipid and phospholipid fractions were higher in the bound lipids than in the free lipids from the various ginseng. The major components were fatty acids, diglycerides and free sterols in neutral lipid fractions, monogalactosyl diglyceride, sterol glucoside and esterified steryl g1ycoside in glycolipid fractions and phosphatidyl glycerol, phosphatidyl ethanolamine and phosphatidic acid in phosphoipid fractions. Seventeen fatty acids were analyzed in the four bound lipid fractions from the various ginsengs and main fatty acids were linoleic acid, palmitic acid and oleic acid. Total saturated fatty acid and palmitic acid contents were higher and total unsaturated fatty acid and linoleic acid contents lower in the total bound lipids than in the total free lipids from the various ginsengs.