• Title/Summary/Keyword: sterilization of microorganisms

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New curing method using gaseous oxidant on sweet potato (Ipomoea batatas)

  • Jin, Hyunjung;Kim, Wook
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2017.06a
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    • pp.39-39
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    • 2017
  • In Asia, sweet potato (Ipomoea batatas) is a very important crop for starch production. Approximately 74.3% of the total sweet potato production quantity is produced in Asia (FAO, 2014) and China is the largest producer of sweet potato. Post-harvest management is particularly important because it is difficult to maintain the quality as well as quantity of sweet potatoes. Despite the importance of post-harvest management, researches on sweet potato have been focused on production-related study such as breeding of new variety, improved techniques of cultivation, so there is limited research on storage after harvest. Curing is a normal practice after sweet potato harvest to promote wound healing and extend postharvest storage life. In Korea, harvested sweet potatoes are usually cured for 4 to 7 days at $30-33^{\circ}C$ and 80-95% relative humidity within one week. Since the optimum storage temperature of sweet potato is regarded as $15-20^{\circ}C$, additional facilities and costs are required to raise the temperature for curing. However, the majority of small farmers do not have the capacity to provide additional facilities and costs. This study was initiated to suggest a new curing method to accelerate the wound healing by applying chemical oxidation to the wound surface of sweet potato. Oxidative stress is known to play an important role in the synthesis of secondary metabolites including lignin. In addition, chemical oxidation can be applied to prevent spoilage caused by microorganisms. Powerful gaseous oxidant with excellent penetration ability and superior sterilization effect was selected for this study. Lignification, weight loss, and spoilage rate of artificially wounded sweet potatoes were investigated after oxidant fumigation. There were clear differences in morphological analysis such as lignification pattern, lignin deposition color, and continuity of lignified cell layers between oxidant-fumigated sweet potatoes and control. These results show that gaseous oxidant can be used to supplement or replace the curing practice, to improve shelf-life as well as curing cost reduction.

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Effects of Ozone Treatment and Gamma Irradiation on the Quality Properties of Dried-Spirullina and Dried-Sea Tangle Powders (오존처리와 감마선 조사가 스피루리나와 다시마 분말의 품질특성에 미치는 영향)

  • Byun, Myung-Woo;Yook, Hong-Sun;Kwon, Oh-Jin;Jo, Sung-Kee;Lee, Sung-Hee
    • Korean Journal of Food Science and Technology
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    • v.29 no.4
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    • pp.764-770
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    • 1997
  • For the purpose of improving hygienic quality of dried-spirullina and dried-sea tangle powders as raw materials of health food, the comparative effects of ozone treatment and gamma irradiation on the microbial and physicochemical properties were investigated. Gamma irradiation at 7.5 kGy resulted in sterilization of total aerobic bacteria, coliforms and molds below detective levels $(<10^{1}\;CFU/g\;sample)$, while ozone treatment for 8 hr up to 18 ppm could not sufficiently eliminate the microorganisms of the samples. Physicochemical properties including compositions of fatty acid and amino acid, minerals, pH and natural pigments were not changed by gamma irradaition up to 10 kGy, whereas, ozone treatment caused changes in pH, TBA value, natural pigments and fatty acid compositions. Especially, ozone treatment markedly decreased unsaturated fatty acid of the samples.

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Inactivation of Vibrio parahaemolyticus by Aqueous Ozone

  • Feng, Lifang;Zhang, Kuo;Gao, Mengsha;Shi, Chunwei;Ge, Caiyun;Qu, Daofeng;Zhu, Junli;Shi, Yugang;Han, Jianzhong
    • Journal of Microbiology and Biotechnology
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    • v.28 no.8
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    • pp.1233-1246
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    • 2018
  • Vibrio parahaemolyticus contamination causes serious foodborne illness and has become a global health problem. As a disinfectant, aqueous ozone can effectively kill a number of bacteria, viruses, parasites, and other microorganisms. In this study, three factors, namely, the aqueous ozone concentration, the exposure time, and the bacterial density, were analyzed by response surface methodology, and the aqueous ozone concentration was the most influential factor in the sterilization ratio. Under low aqueous ozone concentrations (less than 0.125 mg/l), the bacterial cell membranes remained intact, and the ozone was detoxified by intracellular antioxidant enzymes (e.g., superoxide dismutase and catalase). Under high aqueous ozone concentrations (more than 1 mg/l), cell membranes were damaged by the degree of peripheral electronegativity at the cell surface and the concentration of lactate dehydrogenase released into the extracellular space, and the ultrastructures of the cells were confirmed by transmission electron microscopy. Aqueous ozone penetrated the cells through leaking membranes, inactivated the enzymes, inhibited almost all the genes, and degraded the genetic materials of gDNA and total RNA, which eventually led to cell death.

A Study on the Optimization of Ballast Water Treatment System for Scale-up (선박평형수 처리장치의 Scale-up을 위한 조건 최적화 연구)

  • Kang, Ahyoung;Song, Ju Yeong
    • Korean Chemical Engineering Research
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    • v.54 no.5
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    • pp.630-635
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    • 2016
  • In the prior work, we studied a ballast water treatment apparatus, which is secondary pollution free by using physical treatment of shear stress. The principle of this apparatus is smashing various microorganisms by shear stress generated between stationary outer cylinder and revolving inner cylinder. Because of various magnitude of shear stress according to the inner cylinder surface type and revolution speed, an appropriate surface type and optimum revolution speed should be studied by consecutive experiment to determine the reference data for commercial apparatus. Through a comparative study of disk type and cylinder type of ballast water treatment apparatus, cylinder type is turned out to be superb to disk type. In this study, we studied to determine the superior collaboration of cylinder type, groove type and knurling type of inner cylinder to non patterned outer cylinder, and to optimize the revolution speed and flow rate according to the gap between inner cylinder and outer cylinder. As a result, we could get perfect sterilization effect at groove type under the conditions of 250 mL/min of flow rate at 8,000 rpm and 500 mL/min of flow rate at 10,000 rpm respectively.

Plasma Medicine: How can Nonthermal Atmospheric Plasma be Applied to Medicine? (플라즈마 메디신: 저온 상압 플라즈마는 어떻게 의학분야에 적용될 수 있는가?)

  • Park, Sang Rye;Hong, Jin Woo;Lee, Hae June;Kim, Gyoo Cheon
    • Journal of Life Science
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    • v.23 no.6
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    • pp.838-846
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    • 2013
  • As a forth state of material, plasma is ionized gas, which generates characteristically various reactive species. After late of $20^{th}$ century, plasma has been widely used in industry. After nonthermal atmospheric plasma was developed, it has been applied to biomedical fields. Nonthermal atmospheric plasma does not give thermal damages to human tissues, and it shows the high efficiency in cancer treatment, sterilization, tooth bleaching, coagulation, and wound healing. Because the application of plasma to biomedicine has been expanded through interdisciplinary studies, its value of high medical technology is increasing now. Since nonthermal atmospheric plasma was first applied to the mammalian cells and microorganisms, many valuable studies has been performed for about last 10 years, so that now the new research area called 'plasma medicine' has been formed. This article introduces the recent data resulted from plasma medicine and helps to understand the plasma medicine.

Improvement of physiological activity and processing quality through structural transformation of natural biomaterials based on radiation technology (방사선분자변환기술 기반 천연 생물소재 구조변환에 따른 가공적성 및 생리활성 증진 연구)

  • Byun, Eui-Baek;Song, Ha-Yeon;Kim, Hye-Min;Kim, Woo Sik;Lee, Seung Sik;Choi, Dae Seong;Lim, Sang-Yong;Chung, Byung Yeoup
    • Korean Journal of Food Science and Technology
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    • v.50 no.3
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    • pp.249-259
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    • 2018
  • Radiation technology (RT) has long been applied in various fields for increasing the safety and shelf-life of foods by controlling pathogen-induced poisoning. RT was introduced for the first time in Korea in the 1950s to eliminate harmful microorganisms in food materials. In the 1980s, RT had been scientifically proven to be effective for the sterilization of food and public health products. In recent years, irradiation with gamma rays has also been used for improving physiological properties through the structural modification of natural molecules, which has been proposed to be applicable to various industries. In particular, radiation transformation technology (RTT), which involves the development of new functional compounds through the molecular conversion of natural biomaterials, is becoming a new high-value technology as a fusion technique of RT and biotechnology. The present reports have suggested that RTT can be an effective tool for the development of new functional compounds and improvement of the physiological activity of biomolecules.

Sterilization of Garlic Powder by Irradiation (방사선(放射線)에 의한 마늘분말(粉末)의 살균(殺菌))

  • Kwon, Joong-Ho;Byun, Myung-Woo;Cho, Han-Ok
    • Korean Journal of Food Science and Technology
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    • v.16 no.2
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    • pp.139-142
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    • 1984
  • Effects of irradiation on the microbial growth and physicochemical properties of garlic powder were investigated during 3 months storage. Total bacteria and coliform group of garlic powder were $4.74{\times}10^{4}$ and $5.0{\times}10^{3}$ per g, respectively and irradiation of 5 kGy and 7 kGy could sterilize coliform group and total bacteria, respectively. $D_{10}$ value of total bacteria was 3.34 and no microorganisms were grown in 5 to f kGy irradiated groups after 3 months storage at $30{\pm}1^{\circ}C$. Moisture, sugars and pH of garlic powder were not remarkably changed during storage but pyruvic acid content was slightly decreased with storage period. Color difference of garlic powder after 3 months storage could not be distinguished by naked eye, but a slight change was recongized by the mechanical measurement.

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Sterilization and Storage of Onion Powder by Irradiation (방사선(放射線)에 의(依)한 양파분말의 살균(殺菌) 및 저장(貯藏))

  • Byun, Myung-Woo;Kwon, Joong-Ho;Cho, Han-Ok
    • Korean Journal of Food Science and Technology
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    • v.16 no.1
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    • pp.47-50
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    • 1984
  • Effects of irradiation on microbiological, physicochemical aspects and quality of onion powder were investigated during three months storage. Total bacteria and coliform were sterilized by irradiation of 7 and 5 kGy, respectively and no microorganisms were grown after three months storage at $30{\pm}1^{\circ}C$. $D_{10}$ value of total bacteria was 1.64 kGy. Proximate composition of onion powder was not remarkably changed during storage except the slight decrease of pyruvic acid content. Color difference could not be distinguished by naked eye but was slightly changed by the mechanical measurement.

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Nonthermal Sterilization of Pathogenic Escherichia coli by Intense Pulsed Light Using a Batch System (회분식 광펄스 처리에 의한 병원성 대장균의 비가열 살균)

  • Kim, Ae-Jin;Shin, Jung-Kue
    • Korean Journal of Food Science and Technology
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    • v.47 no.1
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    • pp.81-86
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    • 2015
  • Intense pulsed light (IPL), a nonthermal technology, has attracted increasing interest as a food processing technology. However, its efficacy in inactivating microorganisms has not been evaluated thoroughly. In this study, we investigated the influence of IPL treatment on the inactivation of Escherichia coli O157:H7 depending on light intensity, treatment time, and pulse number. Increased light intensity from 500 V to 1,000 V, raised the inactivation rate at room temperature. At 1000 V, the cell numbers were reduced by 7.1 log cycles within 120 s. In addition, increased pulse number or decreased distance between the light source and sample surface also led to an increase in the inactivation rate. IPL exposure caused a significant increase in the absorption at 260 nm of the suspending agent used in our experiments. This indicates that IPL-treated cells were damaged, consequently releasing intracellular materials. The growth of IPL-irradiated cells were delayed by about 5 h. The degree of damage to the cells after IPL treatment was confimed by transmission electron microscopy.

Study on Heat Resistant Putrefactive Spore Formers in Korean Soil and Processed Foods -Part 1. Survey on regional distribution of spore forming bacteria- (가공식품(加工食品)의 내열성부패균(耐熱性腐敗菌) 분포(分布) 조사연구(調査硏究) -(제(第) 1 보(報)) 지역별(地域別) 아포형성균(芽胞形成菌) 조사(調査)-)

  • Koo, Young-Jo;Shin, Dong-Hwa;Kim, Choung-Ok;Min, Byoung-Yong
    • Korean Journal of Food Science and Technology
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    • v.10 no.2
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    • pp.224-230
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    • 1978
  • Heat resistant putrefactive microorganisms causing spoilage of canned and processed foods were surveyed in the compost on mushroom growing bed, casing soil, raw mushrooms and canned products before sterilization at canneries located at 8 places including Buyo in Chung-Cheung-Do and monitored the total count and spore formers from the sample taken. The 9 strains of most severe heat resistant among the selected 140 spore formers were selected and determined D and Z value by TDT method. The most strong heat resistant strain was No. F-10, facultative thermophile, which was isolated from raw mushroom in Buyo area and it's Z value was $21.1^{\circ}F$ (M/15 phosphate buffer solution) and $D^{250}$ was 6.6 min.

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