• 한국정보디스플레이학회:학술대회논문집
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• 2004.08a
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• pp.425-427
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• 2004

Many of the problems by using the microscope are related to the fact that the eyes of the surgeon must be continually fixed to the microscope eyepieces. In this paper, we describe a development of the stereoscopic monitoring system of the stereo microscope for reduced eyestrain or operator fatigue about the long time observations of the microscope. The system consists of the stereoscopic camera part, the stereoscopic image processor device and the polarized light stereoscopic monitor. The left and right images obtained form the two CCD cameras are the same as the eyepiece images. By use of the image processor, the polarized light stereoscopic monitor displayed a real-time stereo microscope images.

• Applied Microscopy
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• v.37 no.2
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• pp.143-146
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• 2007

The objects of the nature have three dimensional (3-D) parameters. The 3-D profiles are embedded on the photographs and microscopic images. To understand 3-D configuration, stereo pair image with thick section is frequently employed. The perception of 3-D images is possible with the aid of stereoscopic glasses, although the expert can perceive 3-D images without the glasses. Anaglyphic stereo images are constructed by various softwares from commercial and freeware. Here we would like to present an easy anaglyphs construction method with Adobe $Photoshop^{(R)}$ based on tilting paired images from high voltage electron microscope. The anaglyphic stereo images constructed revealed the same 3-D perception with conventional stereoscopy. We could zoom in/out the anaglyph image digitally to investigate the detail configuration by real time. This method is expected to contribute to understanding complex structures 3 dimensionally.

• Applied Microscopy
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• v.46 no.2
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• pp.71-75
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• 2016

The scanning electron microscope (SEM) offers two-dimensional (2D) micrographs of three-dimensional (3D) objects due to its inherent operating mechanisms. To overcome this limitation, other devices have been used for quantitative morphological analysis. Many efforts have been made on the applications of software-based approaches to 3D reconstruction and measurements by SEM. Based on the acquisition of two stereo images, a multi-view technique consists of two parts: (i) geometric calibration and (ii) image matching. Quantitative morphological parameters such as height and depth could be nondestructively measured by SEM combined with special software programs. It is also possible to obtain conventional surface parameters such as roughness and volume of biomedical specimens through 3D SEM surface reconstruction. There is growing evidence that conventional 2D SEM without special electron detectors can be transformed to 3D SEM for quantitative measurements in biomedical research.

• Proceedings of the Optical Society of Korea Conference
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• 2007.07a
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• pp.367-368
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• 2007

Dissection of rat hippocampus using high-definition stereoscopic microscope system is demonstrated. Many surgical operation or animal anatomies are anatomized with stereoscopic microscope. With stereoscopic microscope system, the environments of operation is important factor to dissector. Lights, resolution of acquired stereo images, reflected on the liquid, colors and eye fatigue are fatal factors to dissector. We reduced reflections with two incident angle of lights at 45 degree and used a complementary color at the basement and reform the stereoscopic microsystem. Dissector has felt more comfortable after compensation and operation time is more continuous.

• Archives of Plastic Surgery
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• v.50 no.1
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• pp.125-129
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• 2023

Three-dimensional (3D) video exoscopes are high-magnification stereo cameras that project onto monitors mounted in the operating room, viewable from different angles. Outside of plastic surgery, exoscopes have been shown to successfully improve the ergonomics of microsurgery, though sometimes with prolonged operating times. We compare a single surgeon's early experience performing free flap procedures from 2020 to 2021 using either a binocular microscope or a 3D video exoscope. Ten procedures were performed with the standard operating microscope and 8 procedures with the 3D exoscope. The microsurgeon, having minimal prior experience using an exoscope, reported less neck discomfort following the free flap procedures performed with the exoscope compared with the binocular surgical microscope. Total average operating time was comparable between the standard surgical microscope and the 3D exoscope (13.7 vs. 13.4 hours, p = 0.34). Our early experience using a 3D exoscope in place of a standard optical microscope demonstrated that the exoscope shows promise, offering an ergonomic alternative during microvascular reconstruction without increasing overall operating times. Future studies will compare free flap ischemia time between cases performed using the exoscope and the conventional binocular microscope. Medical Subject Headings authorized following words: free tissue flaps; operating rooms; ergonomics; microsurgery.

• Mycobiology
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• v.37 no.1
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• pp.5-9
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• 2009

An Ophiostoma fungus was isolated from a stump of Pinus thunbergii in a forest on the West coast of Korea. Microscopic analysis using a light microscope, a stereo microscope, and a scanning electron microscope revealed that it had morphological features of Pesotum and Sporothix synanarmorphs. Based on the $\beta$-tubulin gene sequence analysis, the fungus was identified as the anamorph of Ophiostoma floccosum. Mycological properties of the species including its growth properties on different culture media were described.

• Applied Microscopy
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• v.43 no.1
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• pp.21-26
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• 2013

The oriental fruit moth (Grapholita molesta Busck) and the peach fruit moth (Carposina sasakii Matsumura) are the most severe insect pests affecting apple orchards in Korea. To prevent an outbreak of these two species and to control these agricultural insect pests, it is important to identify them accurately. However, it is hard to classify them when they were in the larval stage since they tunnel into the apple fruit. In this study, surface structures of the two species of larvae were observed using stereo microscope and scanning electron microscope. Distinct differences between the two species of larvae were found. The prothorax spiracles of oriental fruit moth larvae were approximately twice as large as those of peach fruit moth larvae. The arrangements of subventral setae, located around the proleg, were different between oriental fruit moth and peach fruit moth larvae. Furthermore, subdorsal setae of oriental fruit moth were located next to the spiracle on the 8th abdominal segment, while that of peach fruit moth was located above the spiracle. The identification of the two species of larvae observed in this study was confirmed using polymerase chain reaction-restriction fragment length polymorphism method. Surface structural differences are intrinsic characteristics for each species of larvae and can easily be identified using stereo microscope. These specificities will be helpful where a large number of field-collected larvae need to be identified routinely in pest control research.

• Proceedings of the KIEE Conference
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• 2000.07c
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• pp.1513-1515
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• 2000

In this paper, we studied the $Cu_{2}O$ growing with current at the contacts of copper wires and analyzed surface states and structures, crystalline structures, compositions and thermal characteristics of $Cu_{2}O$ growing wires by using stereo microscope, SEM, metallurgical microscope, EDX and DSC. We expect that the results of this experiment can be applied to the electrical fire investigation.

• Applied Microscopy
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• v.15 no.1
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• pp.86-100
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• 1985

A observation of the ovarian development and oogenesis of Pieris rapae Linne has been carried out during metamorphosis using stereo-microscope, light microscope and electron microscope. The results obtained through this experiment are as follows: 1. The ovarian development and vitellogenesis begin at the 3-day old pupa and the 6-day old pupa respectively, and the adult ovary right after their emergence contains a few mature eggs. 2. The species described above are further observed at six different stages in oogenesis, and the results are summarized as follows. 1) Pieris rapae has polytrophic ovarioles. The cell organelles of the nurse cells are transfered to the oocyte through the ring canal at the early oogenesis. 2) At stage 2, the nuclear envelope of oocyte nucleus is less infolding than that of nurse cell nucleus. In the oocyte cytoplasm a large number of ribosomes are observed. 3) At stage 3 and 4, many micropinocytotic vesicles are observed in the oocyte cytoplasm. These vesicles are fused together to form large proteid yolks. 4) At stage 5, the vitelline membrane is laid down in the intercellular space between the follicle cells and oocyte. 5) At stage 6, the chorion is formed by the follicle cells. 6) A micropyle and a number of aeropyle are observed on the surface of a mature egg.

• Applied Microscopy
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• v.50
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• pp.18.1-18.7
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• 2020

The olfactory anatomy and histology of Lethenteron reissneri were researched using a stereo microscope, a light microscope, and a scanning electron microscope. As in other lampreys, it shows same characters as follows: i) a single olfactory organ, ii) a single tubular nostril, iii) a single olfactory chamber with gourd-like form, iv) a nasal valve, v) a nasopharyngeal pouch, vi) a sensory epithelium (SE) of continuous distribution, vii) a supporting cells with numerous long cilia, viii) an accessory olfactory organ. However, the description of a pseudostratified columnar layer in the SE and Non SE is a first record, not reported in sea lamprey Petromyzon marinus. In particular, both 19 to 20 lamellae in number and olfactory receptor neuron's quarter ciliary length of the knob diameter differ from those of P. marinus. From these results, it might be considered that the olfactory organ of L. reissneri shows well adaptive structure of a primitive fish to slow flowing water with gravel, pebbles, and sand and a hiding habit into sand bottom at daytime. The lamellar number and neuron's ciliary length may be a meaningful taxonomic character for the class Petromyzonida.