• Asian-Australasian Journal of Animal Sciences
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• 제14권2호
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• pp.288-296
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• 2001

The behavior of declawed emus in a farm environment has not been described despite its importance in the husbandry and welfare of the emu. This study examined whether declawing of emus causes chronic pain resulting in permanent changes in the locomotor and general behavior of declawed yearling emus compared to emus not declawed. One group of 40 emus were declawed on the day of hatch by removing the distal phalangeal joint using a Lyon beak-trimming machine. Another group of 40 emus not declawed were the controls. Declawed emus one year of age were allocated to a paddock $250m{\times}125m$, while the control group was placed in an adjoining paddock of the same dimensions. One hour video records of individual emus from each treatment were made from 08:00 and 17:00 h over 2 periods; firstly when food and water was available and secondly during a period when food and water was not available after being withdrawn overnight. Inactive, ingestive, posture change, grooming, aggressive and locomotor behaviors were monitored from the videotape. There was no behavioral evidence to indicate loss of locomotor ability of declawed emus or to suggest declawed emus were suffering from severe chronic pain as indicated by declawed emus engaging in significantly more bouts (p<0.05) and time of searching (p<0.05). Declawed emus also engaged in less stereotype pacing (p<0.05) indicating they were under less stress and not as frustrated as control birds which engaged in more step pushing behavior (p<0.05). Modelling analysis showed that pecking behavior in birds was most closely related to foraging behavior. Birds subject to pecking attacks demonstrated higher levels of stereotype behavior presumably as a method to cope with stress. The behavioral evidence in this study would indicate that declawing does not compromise the locomotor ability of emus and has the benefit of improving the social structure in the groups by reducing stereotype behavior and aggression.

• 한국자기공명학회논문지
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• 제19권3호
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• pp.99-105
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• 2015

Hsp33 is a prokaryotic molecular chaperon that exerts a holdase activity upon response to an oxidative stress at raised temperature. In particular, intramolecular disulfide bond formation between the four conserved cysteines that bind a zinc ion in reduced state is known to be critically associated with the redox sensing. Here we report the backbone NMR assignment results of the half-oxidized Hsp33, where only two of the four cysteines form an intramolecular disulfide bond. Almost all of the resolved peaks could be unambiguously assigned, although the total assignments extent reached just about 50%. Majority of the missing assignments could be attributed to a significant spectral collapse, largely due to the oxidation-induced unfolding of the C-terminal redox-switch domain. These results support two previous suggestions: conformational change in the first oxidation step is localized mainly in the C-terminal zinc-binding domain, and the half-oxidized form would be still inactive. However, some additional regions appeared to be potentially changed from the reduced state, which suggest that the half-oxidized conformation would be an intermediate state that is more labile to heat and/or further oxidation.

• 터널과지하공간
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• 제26권1호
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• pp.12-23
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• 2016

향후 국내에서 수행될 이산화탄소 지중저장 프로젝트의 성공적인 수행을 위해서는 현장 지질조건에 최적화된 주입 조건을 설계하는 것이 중요하다. 따라서 본 연구에서는 주입량 및 주입온도 등의 주입조건이 격리저장시스템의 역학적 안정성에 미치는 영향을 열-수리-역학 연계해석기법의 하나인 TOUGH-FLAC 해석기법을 이용하여 평가하였다. 저장시스템의 역학적 안정성은 기존 균열의 전단미끄러짐 발생가능성을 활동마찰각 및 응력원을 이용하여 분석하였다. 이산화탄소의 주입온도가 저류층의 초기온도보다 낮은 저온주입의 조건에도 열응력으로 인한 인장균열은 발생하지 않는 것으로 파악되었으나, 저류층에서 전단미끄러짐이 발생하는 결과를 보였다. 단위시간당 이산화탄소 주입량을 변화시킨 시나리오 해석에서는 단계별로 주입량을 감소시키는 주입시나리오에서 기존 균열들의 전단미끄러짐 발생 가능성이 가장 낮은 것으로 평가되었다.

• 수산해양기술연구
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• 제37권4호
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• pp.296-301
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• 2001

기존의 솔레노이드 밸브는 전자부품에 기계적 운동요소를 포함하여 비선형성을 내포하고 있으나, MC 성분의 ER유체를 이용하면, 유동체의 통과부분을 전기장 제어를 통하여 솔레노이드 밸브 기능을 대신할 수 있는 메카니즘을 구현할 수 있을 것으로 사료된다. 유기성인 MC성분 ER유체를 유압시스템에 솔레노이드 밸브 역할에 적용하기 위해, 부과하는 직류 전기장의 사이클 수에 따른 기계적특성에 대한 평가는 다음과 같다. MC성분 ER유체의 전단속도비에 대한 전단응력 분포변화는 2.0kV/mm까지 전기장을 인가했을 경우, 횡축의 전단속도가 증가하여도 종축의 전단응력은 거의 변하지 않았다. 60만 사이클을 반복한 후 ITMC25의 전단응력 실험결과, 2.0kV/mm 이상 전기량을 인가하면 2차원적인 곡선의 형태를 형성하지만 표준편차의 평가치가 오차한계 이내이므로 직선으로 판단하여도 무리가 없을 것으로 사료되었다. 구리 전극으로 전기장을 부과한 경우 MC 성분의 ER유체는 0.1~$0.3{\mu}m$까지의 표면거칠기를 나타냈고, 알루미늄 전극을 사용한 경우는 전기장 부과 초기에 $0.3{\mu}m$의 표면거칠기가 $0.2{\mu}m$로 감소하였으나 40만 사이클의 전기장 부과 이후는 약간의 요철변화가 있었다.(이 논문의 결론 부분임)

• 한국작물학회지
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• 제52권4호
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• pp.458-468
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• 2007

To tolerate environmentally adverse conditions such as cold, drought, and salinity, plants often synthesize and accumulate proline in cells as compatible osmolytes. ${\Delta}^1$-Pyrroline-5-carboxylate synthetase(P5CS) catalyzes the rate-limiting step of proline biosynthesis from glutamate. Two complete genes, MtP5CS1 and MtP5CS2, were isolated from the model legume Medicago truncatula by cDNA cloning and bacterial artificial chromosome library screening. Nucleotide sequence analysis showed that both genes consisted of 20 exons and 19 introns. Alignment of the predicted amino acid sequences revealed high similarities with P5CS proteins from other plant species. The two MtP5CS genes were expressed in response to high salt and low temperature treatments. Semi-quantitative reverse transcription-polymerase chain reaction showed that MtP5CS1 was expressed earlier than MtP5CS2, indicating differential regulation of the two genes. To evaluate the reverse genetic effects of nucleotide changes on MtP5CS function, a Targeting Induced Local Lesions in Genomes approach was taken. Three mutants each were isolated for MtP5CS1 and MtP5CS2, of which a P5CS2 nonsense mutant carrying a codon change from arginine to stop was expected to bring translation to premature termination. These provide a valuable genetic resource with which to determine the function of the P5CS genes in environmental stress responses of legume crops.

• 한국지반공학회:학술대회논문집
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• 한국지반공학회 2009년도 춘계 학술발표회
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• pp.108-118
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• 2009

As increasing demand on marine structures and skyscrapers, a deep shaft pile is frequently to be used for the place having weak ground strength. Because heavy horizontal force is generally applied on upper part of pile foundation used in engineering field, steel pile is highly used due to its high resistance to shear force and bending moment, and its capability to carry heavy loads. The steel pile has advantage in good constructibility, high applicability on site and easy handing, but has disadvantage in cost, more expensive than other material pile. This study is to examine the composite pile that makes economical construction possible by reducing material cost of pile; using steel and PHC pile A non welding connection method is applied to this composite pile. This study had step of comparison with the result of numerical analysis after analyzing the result of field test. Numerical analysis is the process of analyzing lateral behavior of non welding composite pile. Moreover, detailed analysis was implemented in order to evaluate joint stability. As a result of the analysis, we could interpret that the stability of the connection part is ensured as seeing the smaller internal stress than approved internal stress. Based on this study, we analyzed lateral behavior of non welding composite pile, which ensured the stability of connection part.

• 한국기계가공학회지
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• 제17권1호
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• pp.34-41
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• 2018

A flange joint is a pipe connection used to prevent the leakage of high-pressure fluids by inserting a gasket and tightening the bolts. Among several kinds of gaskets available, metal ring type joint gaskets are most widely used in conditions that require high-temperature and high-pressure fluid flow, such as oil pipelines, gas pipes, pumps, valve joints, etc. The purpose of this study is to investigate the contact pressure and stress characteristics closely related to the sealing performance of Class 900 flange joints used in high temperature and high pressure environments. The dimensions of flange joints with five different nominal pipe sizes were determined with reference to those specified in ASME 16.5. The metal ring gaskets inserted in the joints were octagonal and oval gaskets. The bolt tensile forces calculated from the tightening torques were input as the bolt pretension loads in order to determine the contact pressure and stress levels after fastening. Loading was composed of three steps, including the fastening step, and different amounts of applied pressures were used in each analysis to investigate the effect of fluid pressure on the contact force of the joints. A general-purpose software, ANSYS 17.2, was used for the analysis.

• 디지털융복합연구
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• 제14권11호
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• pp.427-434
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• 2016

본 연구는 베트남출신 여성결혼이민자의 결혼만족감에 관한 연구로서, 여성의 인구사회학적 변인, 한국인 남편의 인구사회학적 변인, 여성의 긍정적 내적자질로서의 자아효능감, 남편과 지인으로부터 받는 사회적지지, 그리고 문화적응과 관련된 다양한 변인들을 중심으로 결혼만족감에 영향을 미치는 요인이 무엇인지를 검증하였다. 표집방법으로는 의도적 표집(purposive sampling)방법이 사용되었으며, 연구대상자는 서울, 경기 및 충청지역에 거주하는 베트남출신 여성결혼이민자 201명이었다. 4단계로 이루어진 위계적 다중회귀분석 결과 최종모델에서 결혼만족감에 영향을 미치는 변인은 남편의 연령(${\beta}=-.18$, p<.01), 사회적지지(${\beta}=.61$, p<.001), 문화적응 스트레스(${\beta}=-.24$, p<.001)로 나타나 남편의 연령이 젊을수록, 사회적 지지의 수준이 높을수록, 그리고 문화적응 스트레스를 적게 경험할수록 결혼만족감이 유의미하게 높아지는 것으로 검증되었다. 이상의 변인들의 결혼만족감에 대한 설명력은 58.6%로 높게 나타났으며, 베트남여성결혼이민자의 결혼만족감 증진을 위한 사회적 지원방안에 관해 제언하였다.

• Journal of Ginseng Research
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• 제32권3호
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• pp.232-237
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• 2008

Cinnamoyl-CoA reductase (CCR, EC 1.2.1.44) catalyses the reduction of cinnamic acid CoA esters into their corresponding aldehydes, the first step of the phenylpropanoid pathway specially dedicated to monolignol biosynthesis. A cDNA clones encoding CCR have been isolated from Panax ginseng C.A. Meyer and its expression was investigated in response to abiotic stresses. The cDNA, designated PgCCR which is 865 nucleotides long and has an open reading frame of 590 bp with a deduced amino acid sequence of 176 residues. The PgCCR encoded protein possesses substantial homology with CCRs isolated and cloned from other sources; the highest identity (51.8%) was observed with CCR from Tomato (Lycopersicon esculentum). Under various stress conditions, expression patterns of the PgCCR were highly induced in adventitious and hairy roots by several abiotic stresses. These results indicated that PgCCR plays protective role against diverse environmental stresses.

• Wind and Structures
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• 제15권1호
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• pp.17-26
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• 2012

Modeling swirling wakes is of considerable interest to wind farm designers. The present work is an attempt to develop a computational tool to understand free, far-wake development behind a single rotating wind turbine. Besides the standard momentum and continuity equations from the boundary layer theory in two dimensions, an additional equation for the conservation of angular momentum is introduced to study axisymmetric swirl effects on wake growth. Turbulence is simulated with two options: the standard ${\kappa}-{\varepsilon}$ model and the Reynolds Stress transport model. A finite volume method is used to discretize the governing equations for mean flow and turbulence quantities. A marching algorithm of expanding grids is employed to enclose the growing far-wake and to solve the equations implicitly at every axial step. Axisymmetric far-wakes with/without swirl are studied at different Reynolds numbers and swirl numbers. Wake characteristics such as wake width, half radius, velocity profiles and pressure profiles are computed. Compared with the results obtained under similar flow conditions using the computational software, FLUENT, this far-wake model shows simplicity with acceptable accuracy, covering large wake regions in far-wake study.