Browse > Article
http://dx.doi.org/10.5142/JGR.2008.32.3.232

Isolation and Characterization of Cinnamoyl-CoA Reductase Gene from Panax ginseng C. A. Meyer  

Parvin, Shohana (Korean Ginseng Center for Most Valuable Products and Ginseng Genetic Resource Bank, Kyung Hee University)
Pulla, Rama Krishna (Korean Ginseng Center for Most Valuable Products and Ginseng Genetic Resource Bank, Kyung Hee University)
Shim, Ju-Sun (Korean Ginseng Center for Most Valuable Products and Ginseng Genetic Resource Bank, Kyung Hee University)
Kim, Yu-Jin (Korean Ginseng Center for Most Valuable Products and Ginseng Genetic Resource Bank, Kyung Hee University)
Jung, Dea-Yeoung (Korean Ginseng Center for Most Valuable Products and Ginseng Genetic Resource Bank, Kyung Hee University)
Kim, Se-Hwa (Korean Ginseng Center for Most Valuable Products and Ginseng Genetic Resource Bank, Kyung Hee University)
Yang, Deok-Chun (Korean Ginseng Center for Most Valuable Products and Ginseng Genetic Resource Bank, Kyung Hee University)
Publication Information
Journal of Ginseng Research / v.32, no.3, 2008 , pp. 232-237 More about this Journal
Abstract
Cinnamoyl-CoA reductase (CCR, EC 1.2.1.44) catalyses the reduction of cinnamic acid CoA esters into their corresponding aldehydes, the first step of the phenylpropanoid pathway specially dedicated to monolignol biosynthesis. A cDNA clones encoding CCR have been isolated from Panax ginseng C.A. Meyer and its expression was investigated in response to abiotic stresses. The cDNA, designated PgCCR which is 865 nucleotides long and has an open reading frame of 590 bp with a deduced amino acid sequence of 176 residues. The PgCCR encoded protein possesses substantial homology with CCRs isolated and cloned from other sources; the highest identity (51.8%) was observed with CCR from Tomato (Lycopersicon esculentum). Under various stress conditions, expression patterns of the PgCCR were highly induced in adventitious and hairy roots by several abiotic stresses. These results indicated that PgCCR plays protective role against diverse environmental stresses.
Keywords
Panax ginseng; abiotic stress; cinnamoyl-CoA reductase; salicylic acid; semi-quantitative RT-PCR;
Citations & Related Records
연도 인용수 순위
  • Reference
1 McInnes, R., Lidgett, A., Lynch, D., Huxley, H., Jones, E., Mahoney, N. and Spangenberg, G. : Isolation and characterization of Cinnamoyl-CoA reductase gene from perennial ryegrass (Lolium perenne). J. Plant Physiol. 159, 415-422 (2002)   DOI   ScienceOn
2 Bernards, M. A. and Lewis, N. G. : Alkylferulates in wound healing potato tubers. Phytochemistry. 31, 3409-12 (1992)   DOI   ScienceOn
3 Dong, X. and Mindrinos, M. : Induction of Arabidopsis defense genes by virulent and avirulent Pseudomonas syringae strains and by a cloned avirulence gene. Plant Cell. 3, 61-72 (1991)   DOI   ScienceOn
4 Lacombe, E., Van Doorsselaere, J., Boerjan, W., Boudet, A. M. and Grima-Pettenati, J. : Characterization of cis-elements required for vascular expression of the cinnamoyl CoA reductase gene and for protein-DNA complex formation. Plant J. 23, 663-76 (2000)   DOI   ScienceOn
5 Jung, J. D., Park, H. W., Hahn, Y., Hur, C. G., In, D. S., Chung, H. J., Liu, J. R. and Choi, D. W. : Discovery of genes for ginsenoside biosynthesis by analysis of ginseng expressed sequence tags. Plant Cell Rep. 22, 224-30 (2003)   DOI
6 Collazo, P. and Montoliu, L. : Structure and expression of the lignin O-methyltransferase gene from Zea mays L. Plant Mol Biol. 20, 857-67 (1992)   DOI
7 Hawkins, S., Boudet, A. M. : Wound-induced lignin and suberin deposition in a woody angiosperm (Eucalyptus gunnii Hook.): histochemistry of early changes in young plants. Protoplasma, 191, 96-104 (1996)   DOI
8 Lewis, N. G. and Yamamoto, E. : Lignin: occurrence, biogenesis and biodegradation. Annu Rev Plant Physiol Plant Mol Biol. 41, 455-96 (1990)   DOI
9 Nicholson, R. L. and Hammerschmidt, R. : Phenolic compounds and their role in disease resistance. Annual Review of Phytopathology, 30, 369-389 (1992)   DOI   ScienceOn
10 Whetten, R. and Sederoff, R. : Lignin biosynthesis. Plant Cell, 7, 1001-1013 (1995)   DOI   ScienceOn
11 Kim, H. N., In, J. G., Lee, E. K., Yoon, J. H., Lee, M. S., Bae, H. J. and Yang, D. C. : Isolation and characterization of cinnamoyl CoA reductase gene from Codonopsis lanceolata. Plant. Resources, 8, 181-188 (2005)
12 Saitou, N. and Nei, M. : The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol. 4, 406-25 (1987)
13 Fusch, H. and Sacristan, M. D. : Identification of a gene in Arabidopsis thaliana controlling resistance to club root (Plasmodiophora brassicae) and characterization of the resistance response. Molecular Plant-Microbe Interactions, 9, 91-97 (1996)   DOI
14 Lacombe, E., Simon, H., Doorsselaere, J. V., Piquemal, J., Goffner, D., Poeydomenge, O., Boudet, A. M. and Grima-Pettenati, J. : Cinnamoyl CoA reductase, the first committed enzyme of the lignin branch biosynthetic pathway: cloning, expression and phylogenetic relationships. Plant J. 11, 429-41 (1997)   DOI   ScienceOn
15 Dixon, R. A., Lamb, C.J., Masoud, S., Sewalt, V.J. and Paiva, N.L. : Gene. 179, 61-71 (1996)   DOI   ScienceOn
16 Bucciarelli, B., Jung, H. G., Ostry, M. E., Anderson, N. A., and Vance, C. P. : Wound response characteristics as related to phenylpropanoid enzyme activity and lignin deposition in resistant and susceptible Populus tremuloides inoculated with Entoleuca mammata (Hypoxylon mammatum). Can J Bot. 76, 1282-1289 (1998)   DOI