• Journal of Ocean Engineering and Technology
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• v.24 no.2
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• pp.74-81
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• 2010

Ship STEP is the international standard for the exchange of ship modeling data between heterogeneous systems. It is expected that STEP AP218 can be used for seamless data exchange between various CAD/CAM/CAE systems used in the shipbuilding design process. Although the conformance assessment for standards would maximize the performance and confidence about data exchanges, most research has been directed toward interoperability testing. ISO SC4/TC184 only provides the method for conformance testing, and it can be used with test cases on application protocols. Even though standards have been defined for conformance assessment and testing, there is no organization or association. CAD vendors have focused on interoperability testing for evaluation of the performance of their systems. In this paper, the conformance testing criteria for AP218 have been developed with abstract test cases of ship structures. The requested STEP translator was also reviewed with a developed item pool of testing criteria. The criteria methodology would be a guideline for the development of translators and interfaces. The item pool method of testing criteria for conformance assessment would increase performance and efficiency of data translators for Ship STEP and other standards.

• Journal of Korean Society of Forest Science
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• v.100 no.2
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• pp.202-211
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• 2011

The geometric characteristics of step-pool structures and how they are influenced by channel characteristics were investigated in a steep mountain stream in the Experimental Forests of Kangwon National University in Chuncheon, Gangwon-do. Average values of steps for the study reaches were as follows: step spacing, 4.69 m; step height, 0.47 m; step drop, 0.71 m; step-forming particle sizes, 0.68 m; number, 21steps/ 100 m; the ratio of step spacing to channel width, 0.5; and step steepness, 0.13. Relationships between spacing and height of steps and channel gradient showed a negative- and positive correlation, respectively, whereas all geometric variables of steps manifested poor correlation with channel width. Therefore, step steepness, expressed as the ratio of step height to step spacing, increased as channel gradient increased. The ratio of step steepness to channel gradient representing the criterion of maximum flow resistance was 1.2, indicating the channel bed's stable condition. In particular, the relationship between the ratio of step drop to step height and channel gradient showed a significant negative correlation, suggesting the influence of step-pool geometry in trapping sediment and providing an aquatic habitat. Positive correlations also exist between spacing and drop of steps and step particles. Our findings suggest that the dynamics of step-pool structures may strongly control physical and ecological environments in steep mountain streams, so understanding them is essential for stream management.

• Journal of KSNVE
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• v.8 no.2
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• pp.260-266
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• 1998

According to the conventional method of analysis for the seismic sliding of equipment submerged in a pool, in general, only the initial condition of fluid gap is used to estimate the hydrodynamic effect between the two structures throughout the seismic analysis. This is based on the assumption of small displacement relative to the fluid gap thickness during earthquakes. In a narrow fluid gap condition, however, this method may lead to a result of unconservative side. Through example seismic analyses for equipment submerged in a pool of a building, in this paper, it is studied when and how much the sliding response can be underestimated. And method of updating the hydrodynamic effect in each step of time integration is proposed to avoid excessive error in estimation of peak sliding response in such a case.

• Plant Biotechnology Reports
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• v.4 no.3
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• pp.201-211
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• 2010

We present a highly effective T-DNA inserted gene screening method as part of a reverse genetics model system using the Chinese cabbage (Brassica rapa L. spp. pekinensis). Three-step two-dimensional (2D) matrix strategies are potentially accurate and useful for the identification of specific T-DNA inserted mutants from a large population. To construct our Chinese cabbage model, we utilized a forward genetics screening approach for the abnormal phenotypes that were obtained from transgenic plants of Brassica rapa generated with Agrobacteria tumefaciens containing the pRCV2 vector. From one transgenic plant with an abnormal phenotype, we observed that the st1 gene (which is related to senescence-associated process proteins) contained a T-DNA fragment, and that its expression level was decreased. This T-DNA insert was then used as a control to construct an effective screening pool. As a result, the optimum template concentration was found to be 0.1-1 ng in our PCR strategy. For other conditions, positive changes to the Gibbs free energy prevented the formation of oligo dimers and hairpin loop structures, and autosegment extension gave better results for long fragment amplification. Using this effective reverse genetics screening method, only 23 PCR reactions were necessary to select a target gene from a pool of 100 individual DNAs. Finally, we also confirmed that the sequence we obtained from the above method was identical to the flanking sequence isolated by rescue cloning.