• Title/Summary/Keyword: stem end

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Site-Directed Mutation Effect of the Symmetry Region at the mRNA 5'-end of Escherichia coli aeg-46.5 Gene

  • Ahn, Ju-Hyuk;Choe, Mu-Hyeon
    • BMB Reports
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    • v.29 no.1
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    • pp.92-97
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    • 1996
  • The age-46.5 gene of Escherichia coli is induced by nitrate ion and regulated by Fnr, NarL, and NarP during anaerobic growth. aeg-46.5::lacZ fusion gene shows its maximum expression in narL host after two hours of aerobic to anaerobic switch in M9-Glc-nitrate medium. Fnr and NarP act as positive regulators, and NarL acts as a negative regulator. The control region of the aeg-46.5 was identified and the binding sites of regulator proteins have been predicted (Reznikoff and Choe (1993)). It has two symmetry regions. One is located at -52~-37 bp from the anaerobic mRNA 5'-end, which is the binding site of NarL and NarP. The other is located at +37~+56 bp from the 5'-end of mRNA. In this study, the downstream symmetry region from the mRNA 5'-end was investigated by site-directed mutagenesis. The destruction of the symmetry region increases the expression level of aeg-46.5. We propose that the symmetry region interferes with the expression of aeg-46.5 possibly by forming a stem-and-loop structure.

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Reproductive Capacity of the Genus Lespedeza(1) (싸리류(類)의 재생능력(再生能力) (제(第)1보(報)))

  • Han, Young-Chang
    • Journal of Korean Society of Forest Science
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    • v.21 no.1
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    • pp.35-38
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    • 1974
  • In order to investigate reproductive capacity of genus Lespedeza 5 spp,; L. bicolor, L. maximowiczii, L. japonica var. intermedia, L. angustifolioides, and L, cyrtobotrya, their hights, diameter at the ground level, and the number of sprouts were compared with each other at the end of October during investigating years from 1970 to 1972. For measuring of invested items, 6 stocks which the one-year aged stem were cut above ground were randomly planted per square meter with six replications at the spring of 1970. After hights, diameter, and the number of sprouts were measured at the end of October every year, the stem were cut above ground at the end of November from 1970 to 1972. The results are as follows: 1. The reproductive of the stems showed statistically significant differences at 1% level by species and stock ages. L. bicolor showed the best reproductive capacity of them. 2. The sprouting ability was revealed that L. maximowiczii showed the best of them. 3. The reproductive capacity of stem, diameter, and number of sprouts increased with aging of stock up to 4 years on the investigated 5 species.

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Reproductive Capacity of the Genus Lespedeza (II) (싸리류(類)의 재생(再生) 능력(能力) (II))

  • Han, Young Chang
    • Journal of Korean Society of Forest Science
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    • v.35 no.1
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    • pp.15-18
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    • 1977
  • In order to investigate reproductive capacity of genus Lespedeza 5 spp.; L. bicolor, L. maximowiczii, L. japonica var. intermedia, L. angustifolioides, and L. cyrtobotrya, their hights, diameter at the ground level, and the number of sprouts were compared with each other at the end of October during investigating years from 1970 to 1976. For measuring of invested items, 6 stocks which the one-year aged stem were cut above ground were randomly planted per square meter with six replications at the spring of 1970. After hights, diameter, and the number of sprouts were measured at the end of October every year, the stem were cut above ground at the end of November from 1970 to 1976. The results are as follows: 1. The reproductive of the stems showed statistiaclly significant differences at 1% level by species and stock ages. L. bicolor showed the best reproductive capacity of them. 2. The sprouting ability was revealed that L. maximowiczii showed the best of them. 3. The reproductive capacity of stem, diameter, and number of sprouts increased with aging of stock up to 5 years on the investigated 5 species.

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Fruit Quality of 'Wonhwang' Pear Trees with Low-pesticides and In Vitro Regrowth of Stem Cuttings as Affected by Time of Defoliation (시기별 적엽이 저농약 '원황'배의 과실품질과 삽수의 기내 재생장에 미치는 영향)

  • Kim, Byeong-Sam;Cho, Kyung-Chul;Ma, Kyung-Chul;Yun, Bong-Ki;Jung, Seok-Kyu;Han, Jeom-Hwa;Choi, Hyun-Sug
    • Korean Journal of Organic Agriculture
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    • v.23 no.3
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    • pp.469-480
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    • 2015
  • The study was conducted on the effects of time of defoliation on fruit quality of pear (Pyrus pyrifolia Nakai) trees, managing with low-pesticides, and regrowth of stem cuttings in vitro. Treatments included for 40% of uniform defoliation at early-August, end-August, and early-September, as well as control (no defoliation). Defoliation at early-September and control increased growth of water sprouts as well as concentrations of carbohydrates, total nitrogen, and free sugar in one-year old shoots. Defoliation at early-September and control increased fruit yield and mean fruit weight, with high soluble solids content and fruit surface color of $a^*$ observed for both defoliation at end-August and early-September. Defoliation at early-August increased rates of electrolyte leakage in stem cuttings at $-18^{\circ}C$ in vitro. There were no significantly different for germination rates of the cuttings between the treatments at -18 and $-21^{\circ}C$ in vitro, with the highest germination of the cuttings observed for defoliation at early-September and control at $-27^{\circ}C$. Therefore, orchard management should be performed to be minimized for defoliation of the spur leaves until end-August, causing from precipitation and pests.

Effect of Air Humidity and Water Content of Medium on the Growth and Physiological Disorder of Paprika in Summer Hydroponics (여름철 착색단고추 수경재배 시 공중습도 및 배지함수량이 생육 및 과실의 생리장해에 미치는 영향)

  • Rhee, Han-Cheol;Seo, Tae-Cheol;Choi, Gyoeng-Lee;Roh, Mi-Young;Cho, Myeung-Whan
    • Journal of Bio-Environment Control
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    • v.19 no.4
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    • pp.305-310
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    • 2010
  • This study was carried out to investigate the effect of air humidity and water content of medium on the growth and physiological disorder of paprika in summer hydroponics. Treatments were composed of air humidity of control (over 90%) and dehumidification (low 90%) and water content of 80% and 50%. Time domain reflectometry (TDR) sensors and dehumidifier were used in a drip irrigation system and control system of air humidity, respectively. The early growth of paprika was not affected by air humidity but increased by high water content (80%) of medium. Mean fruit weight was reduced at high air humidity and low water content (50%) of medium, but the fruit number per plant and yield were increased at low air humidity. The incidence of brown fruit stem increased with increasing air humidity and water content of medium. Rate of blossom end rot increased in the low water content medium compared with the high water content medium. The nitrogen (N) was higher content in brown fruit stem than normal stem, but calcium (Ca) was lower.

Effect of Water Content in Substrates as According to Growth Stage on the Growth and Yield of Paprika in Summer Hydroponics (여름철 파프리카 수경재배 시 생육단계별 코이어 배지함수량이 생육과 수량에 미치는 영향)

  • Rhee, Han-Cheol;Seo, Tae-Cheol;Choi, Gyoeng-Lee;Roh, Mi-Young;Cho, Myeung-Whan;Kim, Young-Cheol
    • Journal of Bio-Environment Control
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    • v.20 no.4
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    • pp.258-262
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    • 2011
  • This study was carried out to investigate the effect the water content in substrates as according to growth stage on the growth and yield of paprika in summer hydroponics. Treatments of I, II and III were composed of 55-65-60, 50-60-55 and 45-55-50 % in water contents of growth stages, respectively. Time domain reflectometry (TDR) sensors were used in a drip irrigation system. The early growth of paprika was increased by high medium water content of treatment I. Mean fruit weight was not affected by medium water content, but the fruit number per plant and yield were increased at high medium water content of treatment I. The yield of treatment I was higher than that of treatment II and III. The incidence of brown stem fruit, blossom end rot and sunburn was decreased with increasing water content of medium. Occurred in the low water content of medium. The nitrogen (N) was higher content in brown stem fruit than normal stem, but mineral contents such as potassium (K), calcium (Ca), magnesium (Mg) etc. were not affected.

Effects of nanoscale ridge/groovepattern arrayed surface on in vitro differentiation of multi-potent pulp cells derived from human supernumerary teeth

  • Kim, Daehwan;Jo, Hwansung;Lee, Jingu;Kim, Keesung;Roh, Sangho
    • International Journal of Oral Biology
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    • v.38 no.4
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    • pp.161-167
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    • 2013
  • Human dental pulp stem cells (DPSCs) are multi-potent mesenchymal stem cells that have several differentiation potentials. An understanding of thetissues that differentiate from these cells can provide insights for future regenerative therapeutics and tissue engineering strategies. The mesiodens is the most frequent form of supernumerary tooth from which DPSCs can differentiate into several lineages similar to cells from normal deciduous teeth. Recently, it has been shown that nanoscale structures can affect stem cell differentiation. In our presentstudy, we investigated the effects of a 250-nm nanoscale ridge/groove pattern array on the osteogenic and adipogenic differentiation of dental pulp cells from mesiodenscontaining human DPSCs. To this end, the expression of lineage specific markers after differentiation induction was analyzed by lineage specific staining and RT-PCR. The nanoscale pattern arrayed surface showed apositive effect on the adipogenic differentiation of DPSCs. There was no difference between nanoscale pattern arrayed surface and conventional surface groups onosteogenic differentiation. In conclusion, the nanoscale ridge/groove pattern arrayed surface can be used to enhance the adipogenic differentiation of DPSCs derived from mesiodens. This finding provides an improved understanding of the effects of topography on cell differentiation as well as the potential use of supernumerary tooth in regenerative dental medicine.

Suppression by Antibiotics of the Secondary Infection of the Hollow Stalk(Erwinia carotovora var. carotovora)to Tobacco Plants (적심부위 항생제처리에 의한 담배공동병 2차감염 억제효과)

  • 김정화;이영근;박은경
    • Journal of the Korean Society of Tobacco Science
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    • v.3 no.2
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    • pp.131-133
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    • 1981
  • The hollow stalk, caused by Erwinia carotovors var. carotovora, is probaly the most devastating disease to burley tobacco in Korea. There are two modes of infection to tobacco plants: the primary infection along the plant stem from soil and the secondary one from the topped end of plants. The secondary infection rate could be reduced to almost zero by applying an antibiotic, streptomycin sulfate(400 ppm solution), right after topping.

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유선 기저세포와 유선 종양발생에서의 역할

  • 김남득
    • Journal of Life Science
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    • v.3 no.2
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    • pp.72-78
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    • 1993
  • 유선에 존재하는 유선상피 기저세포(mammary epithelial stem cells)의 증거와 정상조직 혹은 종양조직 발생에서 이들의 역할들을 요약한다. 유선의 실질조직에 기저세포가 존재한다는 것은 여러 형태의 이식실험에서 설명되었고 또 기저세포의 표현형적 특징들은 여러 가지의 vonoclonal antibodies에 의해 논증되었다. 이들 연구의 결과들은 유선의 기저세포군이 end bud와 유선의 기저층(basal layer)에 존재한다고 제시하고 있다. 그러나 이들 기저세포들이 유선의 preneoplasias와 neoplasias에서도 존재하는지에 대해서는 아직까지 명확한 대답을 주고 있지 않다. 명확한 결론을 얻기 위해서는 기저세포에만 특이적으로 나타나는 phenotypic marker들을 확인해야 하고 또 이들이 변형(transformation)된 세포군에도 표현되는 지를 확인할 때에야 가능할 것이다.

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