• Title/Summary/Keyword: stem end

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Nucleotide Sequence and Secondary Structure of 16S rRNA from Sphingomonas chungbukensis DJ77 (Sphingomonas chungbukensis DJ77의 16S rRNA 염기서열과 이차구조)

  • Lee Kwan-Young;Kwon Hae-Ryong;Lee Won-Ho;Kim Young-Chang
    • Korean Journal of Microbiology
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    • v.41 no.2
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    • pp.125-128
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    • 2005
  • A 16S ribosomal RNA gene from S. chungbukensis DJ77 has been sequenced. This sequence had a length of 1,502 bp and was extended for 29 bp at 5' and for 37 bp at 3' from the partial sequence (1,435 bp) registered in 2000 year. Besides, 1 bp was newly added near to the 3' end. We made the secondary structure of the 16S rRNA based on E. coli model and found four specific regions. We found constant and variable regions in genus Sphingomonas as the result of multiple alignment of 16S rRNA gene sequences from Sphingomonas spp. and S. chungbukensis DJ77. We found a stem loop structure in S. chungbukensis DJ77, which was only discovered in C. jejuni to date. It showed the structural agreement despite the difference of the sequences from the both organisms. Finally, S. chungbukensis DJ77 belonged to cluster II (Sphingobium) group, after the classification using phylogenetic analysis and nucleotide signature analysis.

Plant Growth and Anatomical Characteristics of Korean Mistel(Viscum album var. coloratum(kom.)Ohwi) (한국산 겨우살이의 생장과 조직해부학적 특성)

  • Park, Chung-Heon;Park, Chun-Geun;Bang, Kyong-Hwan;Park, Hee-Woon;Seong, Nak-Sul
    • Korean Journal of Medicinal Crop Science
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    • v.11 no.2
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    • pp.122-126
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    • 2003
  • This study was conducted to investigate plant growth characteristics and anatomical studies of Korean mistel (Viscum album var. coloratum (Kom.) Ohwi). Parasite position was upper part that has a plenty of sunshine area 5 to 10m apart from the ground, regardless host tree. Stem developed two branch stems from the each basal stem, leaf was grown from the end stems as opposite. Fruit has a light yellow color and berry size was 7 to 8 mm long diameter and it contains seed. Floral bud differentiation initiated with forming rounded dome and arch-shaped floral apex at the beginning stage, and produced heart-shaped sepal following the differentiation of leaf primordia. Floral bud formation processed centeral part first and following bilateral sides were developed in order. Leaf was thick and stoma and guard cell were observed from the two or three inner layers from the epidermis. Mistletoe penetrated cork cambium of host plant and was growing by rooting on xylem. Korean mistel connected to host plant through parasitic organ, haustorium structurally and physiologically.

Anti-hyperlipidemic Effect of Vitex doniana Ethanol Extract in Poloxamer Induced Hyperlipidemia

  • Sheneni, Victor Duniya;Idakwoji, Precius Adejoh
    • The Korean Journal of Food & Health Convergence
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    • v.4 no.4
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    • pp.1-9
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    • 2018
  • To determine the effect of Vitex doniana (leaves stem and root bark) ethanolic extracts on lipid profiles of Poloxamer 407 (P407) induced hyperlipidemic and normal rats. Fifty four mixed sex rats weighing 100-200g were divided into nine groups comprising six animals per group. At the end of the 21 day, the animals were sacrificed and blood sample were collected for determination of serum levels of: Total cholesterol (TC), Triacylglycerides (TAG), High-density lipoprotein cholesterol (HDL-c) and Low-density lipoprotein cholesterol (LDL-c). The studies showed that all induced treated groups significantly (P<0.05) lower serum levels of TC, TAG, LDL-c and significantly (P<0.05) increased HDL-c when compared to the P407 induced hyperlipidemic control. The normal treated groups showed no significant (P>0.05) difference in the serum levels of TC, TAG, LDL and HDL when compared to the normal control group. Calculation of atherogenic risk predictor indices of the induced treated groups showed that all the extracts significantly (P<0.05) lowered the LDL-c/HDL-c, log (TAG/HDL-c) and significantly (P<0.05) increased HDL-c/TC ratio when compared to the P407 induced hyperlipidemic control group. The atherogenic risk predictor indices of normal treated groups showed no significant difference (P>0.05) in LDL-c/HDL-c, Log (TAG/HDL-c) and HDL-c/TC ratio when compared to the normal control group. The study demonstrates the phytotherapeutic effect of Vitex doniana (leaves, stem and root bark) ethanolic extract in poloxamer 407 induced hyperlipidemia.

Molecular Analysis of Promoter and Intergenic Region Attenuator of the Vibrio vulnificus prx1ahpF Operon

  • Lee, Hyun Sung;Lim, Jong Gyu;Han, Kook;Lee, Younghoon;Choi, Sang Ho
    • Journal of Microbiology and Biotechnology
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    • v.25 no.8
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    • pp.1380-1389
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    • 2015
  • Prx1, an AhpF-dependent 2-Cys peroxiredoxin (Prx), was previously identified in Vibrio vulnificus, a facultative aerobic pathogen. In the present study, transcription of the V. vulnificus prx1ahpF genes, which are adjacently located on the chromosome, was evaluated by analyzing the promoter and intergenic region of the two genes. Northern blot analyses revealed that transcription of prx1ahpF results in two transcripts, the prx1 and prx1ahpF transcripts. Primer extension analysis and a point mutational analysis of the promoter region showed that the two transcripts are generated from a single promoter. In addition, the 3' end of the prx1 transcript at the prx1ahpF intergenic region was determined by a 3'RACE assay. These results suggested that the prx1ahpF genes are transcribed as an operon, and the prx1 transcript was produced by transcriptional termination in the intergenic region. RNA secondary structure prediction of the prx1ahpF intergenic region singled out a stem-loop structure without poly(U) tract, and a deletion analysis of the intergenic region showed that the atypical stem-loop structure acts as the transcriptional attenuator to result in the prx1 and prx1ahpF transcripts. The combined results demonstrate that the differential expression of prx1 and ahpF is accomplished by the cis-acting transcriptional attenuator located between the two genes and thereby leads to the production of a high level of Prx1 and a low level of AhpF.

Study into prosodic morphological analysis in Korean and Standard Chinese partial reduplication and contrastive analysis using optimality theory (한국어와 표준 중국어 부분 중첩 의성·의태어에 대한 운율 형태론적 분석의 문제와 최적성 이론적 분석을 통한 대조 분석)

  • Chang, Jae-Woong
    • Cross-Cultural Studies
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    • v.49
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    • pp.275-301
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    • 2017
  • Based on the previous analysis by Wanghongjun(2008), this paper applies Optimality Theory (OT) to the field of partial reduplication in Korea to increase its scientific validity. Toward this end, I propose an alternative analysis of Korean partial reduplication, applied as a single process of prefixation. Reduplicated words by prefixation are divided into two types: with a heavy syllable of the stem, and another with the stem. The two types of partial reduplication are closely related by the sound features. In addition, I discussed Chinese partial reduplication from a prefixation perspective within the framework of OT and performed contrastive analysis of Korean and Standard Chinese processes of reduplication by focusing on constrained rankings. As a result, the alternative analysis showed a systematic relationship among the reduplicated words in Korean and Standard Chinese. Lastly, I explained that the coronal /t/ and liquid /l/ have a special functional hierarchy in both languages. This study can be re-explained based on the framework of OT according to a few major constraints, involving MAX-BR, MAX-IO. Their rankings show the adequacy of analysis.

Hands-on Education Module for Modular Construction, 3D Design, and 4D Schedule

  • Kithas, Kyle A.;Choi, Jin Ouk
    • International conference on construction engineering and project management
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    • 2022.06a
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    • pp.484-491
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    • 2022
  • A paradigm shift in teaching modular construction in higher education and K-12 is proposed as a means to increase the future adoption of the modular construction technique. To this effect, a new education module is presented to STEM educators. This education module is based on LEGOs and directed towards educators in the architecture, engineering, and construction (AEC) industry. The main objectives of the education module are to increase interest and knowledge of modular construction, acknowledge the benefits of using 3D design with 4D scheduling, and create a simulating hands-on educational opportunity. The education module is designed to allow participants to experience a hands-on simulation of modular construction and stick-built construction through building a LEGO project. Participants are challenged to find the advantages and disadvantages in both construction systems first-hand and record their findings. Results are presented from the preliminary testing of this education model on a group of construction management students at the University of Nevada, Las Vegas. Overall, the survey results showed that the LEGO education module was successful at achieving the project's three main objectives: 1) increasing the participants' interest and knowledge of modular construction through an interactive project; 2) increasing the participants' understanding of the benefits of 3D design with 4D scheduling over the use of 2D drawings; and 3) creating a simulating hands-on educational opportunity to help participants compare modular construction to stick-built construction. In the end, this proposed a new LEGO education module addressing the problems identified from this study with more participants.

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Cold Tolerance of Ground Cover Plants for Use as Green Roofs and Walls (옥상 및 벽면녹화용 지피식물의 내한성 비교)

  • Ryu, Ju Hyun;Lee, Hyo Beom;Kim, Cheol Min;Jung, Hyun Hwan;Kim, Ki Sun
    • Horticultural Science & Technology
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    • v.32 no.5
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    • pp.590-599
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    • 2014
  • This study was conducted to compare the cold tolerance of seven ground cover species, Orostachys japonica, Sedum oryzifolium, S. kamtschaticum 'SG1', S. reflexum, S. rupestre 'Blue Spruce', S. spurium 'Green Mental', and S. takesimense, which have been used for green roof and wall systems in Korea. Plants were grown in 10-cm pots and 1 g of tissues at stem-end and crown of each species were kept under either light or dark condition, respectively. For cold tolerance tests, plants were initially left at $4^{\circ}C$ and linearly cooled to 0, -4, -8, -12, -16, and $-20^{\circ}C$ at $-2^{\circ}C{\cdot}h^{-1}$ rate. Low temperature injury and regrowth rates were visually evaluated and assessed by image analysis, respectively. The lethal temperature ($LT_{50}$) of plant species was determined using electrolyte leakage measurements. S. reflexum was the most cold tolerant, showing the most survival at $-16^{\circ}C$, whereas S. oryzifolium and S. takesimense showed low temperature injury at $-8^{\circ}C$. Similar results were found with electrolyte leakage measurements at the stem end. For each species, the crown (Mean $LT_{50}:\;-12.15^{\circ}C$) was more cold tolerant than the stem end (Mean $LT_{50}:\;-10.47^{\circ}C$). In conclusion, S. reflexum and S. rupestre 'Blue Spruce' are recommended for planting in the central region of Korea during late fall and early winter, as they were more cold tolerant and showed more vigorous regrowth than the other tested plant species.

Effect of Water Content in Coir Substrates on the Growth and Yield of Mini-Paprika in Summer Hydroponics (여름철 소과형 파프리카 수경재배 시 코이어 배지함수량이 생육과 수량에 미치는 영향)

  • Rhee, Han Cheol;Choi, Gyoeng Lee;Roh, Mi Young;Jeong, Jae Woan;Cho, Myeung Hwan;Kim, Young Cheol;An, Chul Geun
    • Journal of Bio-Environment Control
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    • v.21 no.3
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    • pp.236-242
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    • 2012
  • This study was carried out to investigate the effect the water content in coir substrates as according to growth stage on the growth and yield of mini-paprika of three varieties 'E 499524' (red color), 'E 499526' (yellow colar) and 'E 499531' (orange colar) in summer hydroponics. Treatments of I, II and III were composed of 55-65-60, 50-60-55 and 45-55-50% in water contents of growth stages, respectively. Time domain reflectometry (TDR) sensors were used in a drip irrigation system. The early growth of mini-paprika was increased by high medium water content of treatment I in all of three varieties. Mean fruit weight was not affected by medium water content, but the fruit number per plant and yield were increased at high medium water content of treatment I. The Yield of treatment I was higher than that of treatment II and III in all of three varieties. The sugar content was increased by low medium water content of treatment in all of three varieties. The incidence of brown-stem fruit, blossom-end rot and sunburn was decreased with increasing water content of medium. Occurred in the low water content of medium. Mineral contents of fruits such as nitrogen (N), potassium (K), calcium (Ca), magnesium (Mg) etc. were not affected in all of three varieties.

Model Development for the Bucking Rate of Japanese Larch by Cut-to-Length Log Usage (낙엽송 원목의 용도별 조재율 모델 개발)

  • Daesung Lee;Yonghee Lee;Jungkee Choi
    • Journal of Korean Society of Forest Science
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    • v.113 no.3
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    • pp.361-371
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    • 2024
  • This study was conducted to develop models of the xylem volume utilization rate and merchantable log utility rate of the Japanese larch (Larix kaempferi [Lamb.] Carrière), a major commercial species in South Korea. To develop these models, models were fitted together for the xylem volume utilization rate and merchantable log utility rate to the total stem volume. Data on the logs bucked by cut-to-length usage in the forestry field were used for modeling, and the tree diameter at breast height (DBH) and small-end diameter of the last log were used as major predictor variables. Considering the difference in the bucking rate between all logs and solely long-length logs, the models for merchantable log utility rate were classified into two types: short- and long-length logs as well as only long-length logs. Consequently, the models were significantly developed, and the predicted values showed high accuracy within the range of observed values. The tables for bucking rate by cut-to-length log usage were finally provided by applying the models of the xylem volume utilization rate and merchantable log utility rate. Because the bucking rate for all logs and long-length logs can be easily calculated with the easy-to-use DBH and the required small-end diameter of the last log, the developed models are highly expected to be applicable for predicting the timber assortment using the cut-to-length log usage.

Comparison of Trajectory of Quality of Life in Patients with High Risk Breast Cancer Undergoing Adjuvant Chemotherapy and Autogenous Bone Marrow Transplantation (보조적 표준 항암 화학요법에 비한 자가조혈모세포 이식 유방암 환자 삶의 질 내용 비교)

  • Lee, Eun-Ok
    • Asian Oncology Nursing
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    • v.1 no.1
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    • pp.5-17
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    • 2001
  • It is known that aggressive treatment of chemotherapy, radiation and autogenous stem cell transplantation is effective for prevention of recurrence in the high-risk breast cancer patients. It was assumed that this procedure takes a longer time and decreases the quality of life more than the standard adjuvant chemotherapy. However, there are few studies comparing the quality of life of patients having bone marrow transplantation and adjuvant chemotherapy. Most of the studies were focused on the quality of life in one point of time, such as only during the early treatment stage, only overall quality of life rather than specific dimensions of the quality of life. The purposes of this study are 1) to identify the difference of the quality of life between two different treatment patterns, adjuvant chemotherapy and autogenous stem cell transplantation: 2) to identify the mostly affected dimension and the periods of time affected by the treatment patterns; and 3) to identify the trajectories of quality of life in each treatment pattern. This is a time series design that measures 4 different points of times. At the beginning of the study, 19 patients were placed in the chemotherapy group and 12 in the group of auto-peripheral blood stem cell transplantation. The inclusion criterion was the advanced disease stage of 3 or over with metastasis of more than 5 lymph nodes. The exclusion criteria were 1) anyone who has metastasis to other organ; 2) anyone who had psychological problems. Ferrell's Quality of Life Scale for Cancer Survivors 41 items on a 10 point scale was used. The QOL-CS includes 4 dimensions, which were labeled physical, psychological, social, and spiritual. The Cronbach‘s alpha of this scale was 0.89. Mann-Whitney U test and Friedman test were used to test each hypothesis. In comparison of the two groups, the quality of life of the bone marrow transplantation group dramatically increased at the 3rd and 6th month after transplantation, while the chemotherapy groups results stayed lower. The most affected dimension of the quality of life at the end of the treatment was the physical dimension. However, it and increased along with time, while the psychological dimension values remained low over the long-term period. Intensive nursing care is needed during the entire period of chemotherapy in all patients having chemotherapy, and is also required for right after cases of bone marrow transplantation.

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