• 한국동물학회:학술대회논문집
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• 한국동물학회 2000년도 한국생물과학협회 학술발표대회
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• pp.53.2-53
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• 2000

No Abstract, See Full Text

• Archives of Pharmacal Research
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• 제28권3호
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• pp.285-289
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• 2005

Two new sulfated saponins designated as certonardosides P$_{2}$ and I$_{3}$ (1 and 2) were isolated from the brine shrimp active fraction of the MeOH extract of the starfish Certonardoa semiregularis. The structures were determined on the basis of spectral analysis. Compounds 1 and 2 were tested for cytotoxicity against five human tumor cell lines (A549, SK-OV-3, SK-MEL-2, XF498, and HCT15), and compound 1 displayed significant cytotoxicity against the SK-MEL-2 skin cancer cell.

• Journal of Microbiology and Biotechnology
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• 제16권9호
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• pp.1405-1409
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• 2006

Polysaccharides from the starfish Asterina pectinifera were assessed in vitro for their chemopreventive potential in human breast cancer. The polysaccharides from A. pectinifera inhibited cell proliferation in the estrogen receptor-positive (MCF-7) and estrogen receptor-negative (MDA-MB-231) human breast carcinoma cell lines. In addition, the polysaccharides were found to be an inhibitor of cytochrome P450 1A1-mediated ethoxyresorufin O-deethylase activity, and caused a dose-dependent inhibition of aromatase activity in microsomes isolated from a human placenta. There was a significant reduction in the ornithine decarboxylase activity to 30.7% of the control in the polysaccharide-treated MCF-7 breast cancer cells. Therefore, the polysaccharides from A. pectinifera merit further investigation with respect to breast cancer chemoprevention.

• 약학회지
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• 제43권6호
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• pp.771-775
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• 1999

The antimutagenic activities of the total extract and several fractions of starfish, Asterina pectinifera (Asteriidae), were investigated in vitro by SOS chromotest with Escherichia coli PQ37 and Ames test with Salmonella typhimurium TA100. When various fractions was tested, the chloroform and butanol fractions showed low induction factors, which means both fractions increased antigenotoxicity against the base substitution mutagen MNNG. Even though higher antigenotoxic effect of the chloroform fraction, no effective result of Ames test was found in revertant formation of S. typhimurium TA100. The most effective antigenotoxic and antimutagenic fraction was a butanol one: i.e., When 0.5 mg/tube of butanol fraction was applied, the induction factor was 0.68. As the concentration of the fraction was increased the formation of revertants of S. typhimurium TA100 by about 81%. There was no cytotoxic effect of butanol fraction against S.typhimurium TA100. This result might be useful for further study to search a possible anticancer agent from the starfish, Asterina pectinifera.

• Bulletin of the Korean Chemical Society
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• 제26권9호
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• pp.1457-1460
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• 2005

• Bulletin of the Korean Chemical Society
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• 제35권8호
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• pp.2335-2341
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• 2014

Chemical investigation on the methanol extract of the starfish Ctenodiscus crispatus resulted in the isolation of five steroids, (22E,$24{\zeta}$)-26,27-bisnor-24-methyl-$5{\alpha}$-cholest-22-en-$3{\beta}$,5,$6{\beta}$,$15{\alpha}$,25-pentol 25-O-sulfate (1), (22E,24R,25R)-24-methyl-$5{\alpha}$-cholest-22-en-$3{\beta}$,5,$6{\beta}$,$15{\alpha}$,25,26-hexol 26-O-sulfate (2), (28R)-24-ethyl-$5{\alpha}$-cholesta-$3{\beta}$,5,$6{\beta}$,8,$15{\alpha}$,28,29-heptaol-24-sulfate (3), (25S)-$5{\alpha}$-cholestane-$3{\beta}$,5,$6{\beta}$,$15{\alpha}$,$16{\beta}$,26-hexaol (4), and ${\Delta}7$-sitosterol (5). Their structures were identified by extensive spectroscopic analyses, including 1D, 2D NMR and MS and chemical methods. Compound 4 showed cytotoxicity against human hepatoma HepG2 and glioblastoma U87MG cells via inhibition of cell growth and induction of apoptosis. Induction of apoptosis by 4 was demonstrated by cell death, DNA fragmentation, increased Bax/Bcl-2 protein ratio and the activation of caspase-3, caspase-9 and poly (ADP-ribose) polymerase (PARP).

• 한국해양바이오학회지
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• 제1권4호
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• pp.298-304
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• 2006

In this study, we establish a thin-layer chromatography (TLC) immunostaining method for detecting starfish gangliosides. A new monoclonal antibody (MAb) against AG-2, the major gangliosides molecular species of Acanthaster planci, was produced by fusing hybridoma with splenocytes immunized to liposomal AG-2. BALB/c male mice were injected with liposomal AG-2 antigen, and immunized. Their splenocytos were isolated and fused with hypoxanthine-aminopterine-thimidine (HAT)-sensitive mouse myeloma cells. Hybridomas producing MAb reactive to AG-2 were cloned using the limited dilution method. Established hybridomas were cultured in eRDF medium. Crude MAb produced from clone 8D4 was purified with a magnesium pyrophosphate column. Enzyme immunoassay and TLC immunostaining of AG-2 were performed using the purified MAb. Structurally related gangliosides did not cross-react with anti-AG-2 antibodies. The detection limit of TLC immunostaining was 50 ng of AG-2. The newly established immunostaining method was further developed for detecting AG-2 distribution and qualitative analysis in tissues and/or organs. Our results show that the majority of AG-2 is present in the stomach of male A. planci, while AG-2 is distributed not only in the stomach but also in the the pyloric caeca of female A. planci.

• 생명과학회지
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• 제28권8호
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• pp.955-961
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• 2018

불가사리를 이용하여 다양한 생리활성에 대한 연구가 많이 진행되었지만, 다른 천연물 연구에 비해 불가사리로 부터 분리한 미생물에 대한 연구는 부족하다. 이에 본 연구에서는 제주도에서 채집한 극피동물인 빨강불가사리(Certonardoa semiregularis)의 내장으로부터 Marine Agar와 R2A를 이용하여 총 103개의 균주를 분리하여 세균군집에 대해 조사하였다. 분리된 균주들은 16S rRNA유전자를 이용하여 염기서열을 분석 및 동정하였다. 그 결과 주요 계통군은 Proteobacteria (Alpha-proteobacteria 24%, Beta-proteobacteria 4%, Gamma-proteobacteria 65%) 93%, Bacteroidetes 4%, Actinobacteria 2%, Firmicutes 1%로 4개의 문이 확인되었고, 7개의 강(Actinobacteria, Flavobacteria, Bacilli, Sphingobacteria, Alpha-proteobacteria, Beta-proteobacteria, Gamma-proteobacteria), 15개의 목, 19개의 과, 24속이 관찰되었다. 또한 계통 분석 결과 2개의 균주(Lysobacter sp., Pedobacter sp.)가 각각 97.55%, 97.58%로 상동성이 98% 이하로 나타나 새로운 속 또는 종으로 분류될 가능성이 있다고 여겨지며, 표준 균주와 함께 신종 후보 균주에 대한 생화학적, 형태학적 등의 추가적인 신종실험을 향후 진행해야 할 것으로 사료 된다.

• 생명과학회지
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• 제24권8호
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• pp.860-864
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• 2014

별불가사리(Asterina pectinifera)의 유문 맹낭 추출물로부터 새로운 항균활성 펩타이드를 정제하기 위해서 유문 맹낭 추출물을 역상 HPLC와 이온 HPLC column에 주입하였다. 강한 항균활성을 나타내는 2개의 새로운 펩타이드가 유문 맹낭 추출물로부터 정제되었다. 이러한 물질들은 Pyloric caeca Asterina pectinifera peptides (PAP-1와 PAP-2)라 명명하였다. 정제한 물질들의 특성을 알아보기 위해서, 분자량 및 아미노산 서열 분석은 MALDI-TOF 질량분석기와 에드만 분해법으로 조사하였다. PAP-1과 PAP-2의 분자량은 각각 약 2952 Da 및 2980 Da이었다. PAP-1과 PAP-2의 부분적인 N-말단 서열은 다음과 같다. PAP-1, AIQNAGES; PAP-2, AIQNAAES. PAP-2는 PAP-1의 6번째 위치(glycine or alanine)에서 한 잔기만 다른 isoform에 해당된다. 지금까지 밝혀진 항균활성 펩타이드와의 분자량 및 N-말단 아미노산 서열을 비교한 결과, 이들 물질들은 다른 물질들과 동일성을 나타내지 않았다. 이러한 발견은 PAP-1과 PAP-2가 별불가사리의 유문맹낭의 선천성 방어계에 중요한 역할을 담당하고 있는 것을 시사하고 있다.

• 한국정밀공학회:학술대회논문집
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• 한국정밀공학회 2011년도 춘계학술대회 논문집
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• pp.291-292
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• 2011