• 대한약학회:학술대회논문집
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• 대한약학회 2002년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.1
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• pp.197.1-197.1
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• 2002

• 한국수산과학회지
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• 제48권4호
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• pp.432-438
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• 2015

This study examined the antimicrobial, antioxidant, and tyrosinase inhibitory activities of bioactive compounds extracted from two starfish, Asterina pectinifera and Asterias amurensis, using solvent extraction after $Protamex^{TM}$ hydrolysis. Methanol and acetone fractions collected by stepwise extraction from specimens were subjected to silica gel column chromatography (SGCC) (200 mesh and 400 mesh), followed by high-performance liquid chromatography (HPLC). Two fractions (7:3 and 5:5 chloroform : methanol ratio, v/v) eluted using silica gel column chromatography from the two starfishes showed higher antimicrobial activity against Propionibacterium acnes and dermatophyte fungi (Epidermophyton floccosum, Microsporum audouinii, Trichophyton ferrugineum, Trichophyton mentagrophytes, and Trichophyton rubrum), antioxidant activity ($EDA_{50}$, mg/mL), and tyrosinase inhibitory activity compared to the other fractions. The final fractions obtained from Asterina pectinifera (RT 7.53, 8.93, and 10.48 min) and Asterias amurensis (RT 5.02 min) by SGCC (400 mesh) and HPLC from two SGCC fractions (200 mesh) showed 8.94 and 15.59 mg/mL antioxidant activity ($EDA_{50}$) and 46.89 and 40.19 % tyrosinase inhibitory activity, respectively. Extracts from starfishes are potential cosmetic basic material.

• 한국인터넷방송통신학회논문지
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• 제13권2호
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• pp.67-75
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• 2013

우리나라는 삼면이 바다인 환경적 특성으로 인해 연안 양식 산업이 발전해 왔다. 번식력과 포식성이 매우 강한 아무르불가사리에 의해 우리나라 근해의 양식 산업이 받는 피해가 매년 급증하고 있다. 더욱이 아무르불가사리는 살아있는 어패류를 잡아먹기 때문에 양식 어민들의 피해가 매우 크다. 이 논문에서는 수중에서 획득한 아무르불가사리 영상에서 효과적으로 특징을 추출하는 방법을 제안하였다. 제안한 방법에서는 6-방향 스캐닝을 이용한 볼록 특징 추출을 사용하여 기존 방법에 비해서 적은 수의 특징 후보를 산출한다. 또한, 추출된 볼록 특징과 영역 중심점을 이용하여 후보 오목점을 선정한 후 최종 오목 특징을 추출한다. 군집 생활을 하는 불가사리의 특성 때문에 입력 영상의 불가사리 개체도 밀집되어 있다. 따라서 입력 영상에서 추출되는 특징 후보의 수를 최소화하는 것은 중요한 의미를 갖는다. 실험결과, 제안한 특징 추출 방법은 특징 후보수 대비 특징 추출률이 약 88%로 기존 방법에 비해 개선되었다.

• 한국정보통신학회논문지
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• 제17권4호
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• pp.922-934
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• 2013

우리나라 패류 양식장에 큰 피해를 주는 것으로 알려진 아무르불가사리는 잠수부나 어망에 의해 여러 구역을 더듬어서 구제하고 있는 실정이다. 이를 좀 더 효과 적인 방법에 의해 빠른 시간에 많은 량의 아무르불가사리를 구제하기 위해서는 불가시리 인식이 불가피하다. 현재 불가사리 영상 획득 방법은 열악한 수중 환경에서 가시광선 영역을 이용하여 어두운 해저로부터 칼라 영상을 획득하는 데는 한계점이 있다. 이 논문에서는 이러한 문제점을 해결하기 위해서 수중에서 투과력이 우수하고 수중 부유물 잡음에 강한 적외선 영상을 이용하며, 획득된 적외선 수중 영상으로부터 불가사리 인식률을 높이기 위하여 전처리 과정에서 최적 적응형 필터 설계 방법을 제안하고, 제안한 적응형 필터를 이용한 아무르불가사리의 전체 인식률은 88.7%로 만족한 인식 결과를 얻게 되었다.

• Fisheries and Aquatic Sciences
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• 제12권1호
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• pp.1-5
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• 2009

Crude saponin, extracted from the starfish Asterias amurensis, was assessed for its capacity as a biological pesticide. As part of this analysis, its insecticidal and repellent activities, in addition to its acute and chronic toxicities were tested. In comparison with the control group, insecticidal activity of saponin against tobacco cutworm, Spodoptera litura, in kale, Brassica loeracea was low at 36.4%. Repellent activities of the extracted saponin against green peach aphid, Myzus persicae, and S. litura, on soybean leaf, Glycine max and kale were 65.6% and 35.0% at $1^{st}$ day, and 54.5% and 30.0% at $3^{rd}$ day, respectively. Acute and chronic toxicity analysis was carried out using acute immobilizatioin test and reproduction impairment test, respectively. The saponin had 48 h-$EC_{50}$ of $65.21{\mu}g/mL$. Twenty-one day accumulative reproduction after treatment was lower in Daphnia magna at $7{\mu}g/mL$ saponin (78 youngs), compared with the control group (129 youngs). These results indicate that the extracted saponin exhibited some toxicity and has potential as a repellent against insects.

• 약학회지
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• 제41권4호
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• pp.421-432
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• 1997

A new lectin was partially purified from starfish,Asterina pectinifera by means of physiological saline extraction, salt fractionation, ion exchange chromatography and hy droxyapatite chromatography, and it was named APL. The biochemical properties of the APL were characterized. In addition, its effects on lymphocyte mitogenicity and cancer cell agglutinability were tested. The APL agglutinated nonspecifically human erythrocytes and rabbit blood cells. Agglutinability was decreased to 30% of control activity below pH 5 and above pH 9 and was relatively unstable at increasing temperatures above 60$^{\circ}C$. The activity was reduced by addition of two kinds of metal ions, $Ba^{2+},\;Mn^{2+}$ and chelating agent, EDTA. APL was proved to be glycoproteins containing 9% sugars. For carbohydrate specificity, it was found that the activity of APL was inhibited by D(+)-glucosamine, D(+)-galactosamine, stachyose, N-acetyl-galactosamine and methyl-${\alpha}$-D-galactopyranoside among 35 sugars tested. In amino acid composition, the contents of acidic amino acids such as aspartic acid and glutamic acid were relatively high. This result suggest that the isoelectric point would be in a lower range. APL was found that it promotes the division of human lymphocytes. APL was proved to be a potent agglutinin for cancer cells such as HeLa, L929 and L1210 cells. Significant changes on the HeLa cell surfaces affected by APL were observed under the electron microscope.

• 비파괴검사학회지
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• 제2권2호
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• pp.9-16
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• 1983

There are two weak points in a high pressure vessel, one is a corner, the other is a crack on the base. In order to evaluate safety of a department of a corner and a crack like a starfish on the base in a high pressure vessel (working pressure: $130kg/cm^{2}$), which was made by Marison's Process, we analyzed stress by strain gauge, measured thickness and hardness by ultrasonic testing, and were able to test pressure by water pressure from nondestructive testing. Also destructive testings were applied to measure thickness and to observe microstructure and chemical composition of a corner on the base. From the results of the experiment, values of experiment were satisfied with a condition of application. But, it is considered that a crack on the base is to be investigated with more by Fracture Mechanics.

• 한국가축번식학회지
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• 제22권3호
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• pp.277-286
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• 1998

The effect of MAP kinase activity on maturation of porcine oocytes was investigated. MAP kinase was detected by immunofluorescence staining in nucleus of oocytes just before entering GVBD (germinal vesicle breakdown)stage. In a Western blot with GV (germinal vesicle) from these oocytes (cultured for 25 hours), a shift of MAP kinase band a, pp.ared, suggesting an activated stage of the kinase. No activity was shown in the blot with GV isolated from, oocytes cultured for 0 hour. To confirm that activation of MAP kinase induce GVBD, we microinjected MAP kinase purified from matured oocytes starfish into the cytoplasm of oocytes in GV stage (cultured for 0 hour). The injected MAP kinase did not cause early a, pp.arance of GVBD. No oocytes showed GVBD state until 20 hours of culture. Activity of MAP kinase did not increase significantly after the injection. When the exogenous MAP kinase was injected into GV, GVBD was induced in about 20% of oocytes cultured for 5 to 10 hours. These results suggest that MAP kinase is traslocated to nucleus and function as a factor inducing GVBD.

• 한국동물학회지
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• 제37권2호
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• pp.203-212
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• 1994

별불가사리 난자형성 중 난모세포(직경 7-l7O Um) 표면의 구조적 변화를 투과 전자현미경으로 관찰하였다. 직경 25-855 Um의 난모세포에서 'endocvtosis'와 관련된 구조인 coated pit, coated vesicle과 내포소낭이 존재하였으며. 내포소낭은 난황립과 융합하였다. 특히, 이러한 융합은 직경 130-155 Um의 난모세포에서 많이 관찰되었다 난자형성 초기에 난모세포의 미세융모는 세포막을 따라 무질서하게 분포하고. 인접한 세포와 맞닿아 있었다. 직경 15 Um에서 65 Um에 이르는 난모세포는 세포막 부위에 따라서 미세융모의 굵기와 길이, 그리고 형태가 다르게 나타났고, 직경 100 Um 이상의 난모세포에서는 잘 발달된 미세음모가 난뢍층에 존재하였다 여포세포로부터 나온 돌기는 직경 7 Um의 난모세포와 난자형성 후기의 난모세포에서 난황층을 윤고 세포막과 닿아 'junctional complex'를 형성하였다. 이와같은 난모세포 표면의 다양한 구조적 변화는 난모세포의 세포막을 통한 물질투과 기능의 분화와 관련이 있을 것으로 생각된다.

• 환경생물
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• 제22권3호
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• pp.376-380
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• 2004

In August 2003, the water quality of offshore waters along the Incheon coast of Korea was evaluated by biological evaluation using gametes, embryos and early development systems of a starfish species (Asterina pectinifera). As the result of performing biological evaluations on seawater samples from a total thirteen sites, the formation rate of normal larva was 16-68%. At seawater sample from site 5 and 13, formation rate of normal larva averaged 16%, the most abnormal rate hindering the early embryo development of the experimental animal, while that of site 3 averaged 68%, the highest formation rate of normal larva. At seawater sample from site 2, 4, 7, 9, 10, 11, 12, formation rate of normal larva averaged 33-54%, those which damage the development of early embryos slightly. At seawater sample from site 1, 5, 6, 8, 13, formation rate of normal larva averaged 16-28%, those which damage the development of early embryos strongly.