• Title/Summary/Keyword: starch-fermentation

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Screening of Giberella sp. from the Korean Paddy Field for the roduction of Gibberellic Acid and its Cultural Properties (Gibberellic acid를 생산하는 분리주 Gibberella sp. 의 배양학적 특성)

  • 오영준
    • Microbiology and Biotechnology Letters
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    • v.23 no.2
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    • pp.119-122
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    • 1995
  • A different form from Gibberella fujikuroi was isolated from the paddy field of Naju area. The strain, designated as Y107, was identified as Gibberrella sp. based on its morphological, physiological, and biochemical characteristics. The highest production of Gibberellic acid by the strain was achieved in a fermentation medium containing corn starch, glucose, soybean oil, soybean meal, NH$_{4}$NO$_{3}$, K$_{2}$HPO$_{4}$, MgSO$_{4}$, and trace elements.

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전분을 이용한 itaconic acid의 생산

  • Jeong, Sang-Yun;Na, Yeong;Lee, Jong-Il
    • 한국생물공학회:학술대회논문집
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    • 2002.04a
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    • pp.228-231
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    • 2002
  • Itaconic acid has been produced during the cutivation of Aspergillus terreus DSMZ 5770 by using several starchs as carbon sources. The starchs were pretreated by partial hydrolysis with some acids at various pH conditions. The highest yield for the production of itaconic acid has been found when rice starch was pretreated by sulfonic acid at pH 2.5 and utilized for the cultivation. Using the results from shaker fermentation A. terreus has been cultivated in 2.5 L bioreactor for the production of itaconic acid and its on-line monitoring.

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Improvement of Fungal Cellulase Production by Mutation and Optimization of Solid State Fermentation

  • Vu, Van Hanh;Pham, Tuan Anh;Kim, Keun
    • Mycobiology
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    • v.39 no.1
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    • pp.20-25
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    • 2011
  • Spores of Aspergillus sp. SU14 were treated repeatedly and sequentially with $Co^{60}$ ${\gamma}$-rays, ultraviolet irradiation, and N-methyl-N'-nitro-N-nitrosoguanidine. One selected mutant strain, Aspergillus sp. SU14-M15, produced cellulase in a yield 2.2-fold exceeding that of the wild type. Optimal conditions for the production of cellulase by the mutant fungal strain using solid-state fermentation were examined. The medium consisted of wheat-bran supplemented with 1% (w/w) urea or $NH_4Cl$, 1% (w/w) rice starch, 2.5 mM $MgCl_2$, and 0.05% (v/w) Tween 80. Optimal moisture content and initial pH was 50% (v/w) and 3.5, respectively, and optimal aeration area was 3/100 (inoculated wheat bran/container). The medium was inoculated with 25% 48 hr seeding culture and fermented at $35^{\circ}C$ for 3 days. The resulting cellulase yield was 8.5-fold more than that of the wild type strain grown on the basal wheat bran medium.

Optmization of Culture Conditions and Nitrogen Sources for Production of Erythritol by Candida magnoliae. (Candida magnoliae에 의한 에리스리톨 생산을 위한 최적 배양환경과 질소원 선별)

  • 고은성;문관훈;한기철;유연우;서진호
    • Microbiology and Biotechnology Letters
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    • v.28 no.6
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    • pp.349-354
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    • 2000
  • Culture conditions and nitrogen sources were optimized for production of erythritol, a natural sweetener, by Candida magnoliae M26. The optimal culture conditions were found to be culture temperature of $28^{\circ}C$, initial pH of 7, aeration of 1 vvm and agitation speed of 500 rpm in a 2.5 1 jar-fermentor. Glucose was chosen as the best carbon cource bsed on cell growth and erythritol productivity. Kight steep water(LSW) and corn steep liquor (CSL) which are by-products in starch processing from corn were tested as a nitrogen source substitute for yeast extract. The use of either LSW or CSL did not change the fermentation performance. The experimental results using LSW and CSL showed 1.5 times higher in cell growth and almost the same value in erythritol productivity com-pared with the control fermentation using yeast extract as a nitrogen source. These results suggested that either LSW of CSL could be used as a nitrogen source in a large-scale fermentation for erythritol production.

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Development of a Mixed-culture Fermentation Process and Characterization for New Oligosaccharides and Dextran Using Lipomyces starkeyi and Leuconostoc mesenteroides (Lipomyces starkeyi와 Leuconostoc mesenteroides의 혼합배양에 의한 올리고당과 Dextran의 생성 및 생성당의 특성 연구)

  • 허수진;김도만;이인수;장판식
    • Microbiology and Biotechnology Letters
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    • v.27 no.4
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    • pp.304-310
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    • 1999
  • We have developed a new process for the production of new structure oligosaccharides using the mixed-culture fementation of Lipomyces starkeyi KSM22 and leuconostoc mesenteroides B-512FMCM.L.starkeyi KSM22 produces a novel DXAMase(an enzyme containing both dextranase and amylase activities). It hydrolyzes the soluble starch and dextran. The hydrolyzates were used as acceptors for dextransucrase of L.mesenteroides to synthesize the new oligosaccharides(NOS). In fermentation, as the concentration of sucrose was increased from 9%(w/v) to 15%(w/v), the yields of dextran(sum of dextran I, MW=66kD, and dextran II, MW=21kD) was increased from 12.7% to 42.5%, and NOS was increased from 3.9% to 5.2% of the theoretical, respectively. The NOS of dp(degree of polymerization) 5 and over was increased from 33.1% to 58.3% of the total NOS. The NOS showed heat resistant up to 12$0^{\circ}C$ and was stable at pHs ranged from 2 to 6. The NOS decreased the pH changes in the culture of S. mutans, and also showed inhibitory effects on the growth of S. aureus or S. typhimurium.

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Bifidus Fermentation Increases Hypolipidemic and Hypoglycemic Effects of Red Ginseng

  • Trinh, Hien-Trung;Han, Sang-Jun;Kim, Sang-Wook;Lee, Young-Chul;Kim, Dong-Hyun
    • Journal of Microbiology and Biotechnology
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    • v.17 no.7
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    • pp.1127-1133
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    • 2007
  • Antihyperlipidemic and antihyperglycemic effects of Red Ginseng (RG, steamed and dried root of Panax ginseng C.A.Meyer, family Araliaceae), major component of which is ginsenoside Rg3, and Bifidodoterium-fermented RG (FRG), major component of which is ginsenoside Rh2, were investigated. Orally administered RG and FRG potently reduced the serum triglyceride levels in com-oil-induced hypertriglycemidemic mice as well as total cholesterol and triglyceride levels in Triton WR-1339-induced hyperlipidemic mice. Of the saponin and polysaccharide fractions of RG and FRG, the polysaccharide fraction inhibited postprandial blood glucose elevation of maltose- or starch-loaded mice and reduced the blood triglyceride levels in com-oil-induced hypertriglycemidemic mice. The saponin fraction and its ginsenosides Rg3 and Rh2 reduced blood triglyceride and total cholesterol levels in Triton WR1339-induced hyperlipidemic mice. The inhibitory effect of FRG and its main constituents against hyperlipidemia and hyperglycemia in mice were more potent than those of RG. These findings suggest that hypolipidemic and hypoglycemic effects of RG can be enforced by Bifidus fermentation and FRG may improve hyperlipidemia and hyperglycemia.

Fermentation Properties of Amylase Activity and Added Rice Yogurt of Enterococcus faecium KHM-11 Isolated from Korean Human Milk (한국인 모유로부터 분리한 Enterococcus faecium KHM-11의 Amylase 활성과 쌀 첨가 요구르트의 특성)

  • Bae, Hyoung-Churl;Lee, Jo-Yoon;Renchinkhand, Gereltuya;Nam, Myoung-Soo
    • Korean Journal of Agricultural Science
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    • v.37 no.3
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    • pp.387-392
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    • 2010
  • This studies were carried out to assess fermentation properties of amylase activity and added rice yogurt of Enterococcus faecium KHM-11 isolated from Korean human milk. The amylase activity of Enterococcus faecium KHM-11 was above 23 units. Characterization of carbohydrate fermentation of Enterococcus faecium KHM-11 has D-ribose, D-lactose, L-arabinose and starch. Titratable acidity and viable count of lactic acid bacteria of 4% rice yogurt was higher compared to the 0%. Therefore we were discussed Enterococcus faecium KHM-11 is suitable microorganism for fermented milk added rice powder. Hydrolysates of sugars of fermented milk with 4% rice powder cultured Enterococcus faecium KHM-11 were analyzed by TLC and HPLC. Hydrolysates of lactose and galactose were revealed and hydrolysates of glucose was not revealed in results of TLC and HPLC.

Quality Characteristics of Bread Dough added with Immature Chalssalbori Flour (미성숙 찰쌀보리가루를 첨가한 식빵반죽의 품질특성)

  • Kim, Ye-Seul;Park, Ju Yeon;Lee, Jeung Hee
    • Korean journal of food and cookery science
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    • v.30 no.4
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    • pp.385-393
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    • 2014
  • This study investigated both the chemical and antioxidative characteristics of wheat flour added with 10, 20 and 30% immature chalssalbori flour; further, the quality characteristics of bread dough during yeast fermentation were assessed. As the addition rate of chalssalbori flour into wheat flour increased, the DPPH radical scavenging capacity, water absorption index, water solubility index and viscosity were all increased. Moreover, the bread dough with an increased addition rate of chalssalbori flour demonstrated an increased pH, reduced fermentative expansion power and a darker yellowish color. The bread dough added with chalssalbori flour after mixing showed higher hardness, gumminess and adhesiveness, but lower cohesiveness and springiness compared to the control; however, these texture characteristics were changed during fermentation. In the present study, the mixed flour with 10% immature chalssalbori flour conveyed similar pasting characteristics to those of wheat flour; moreover, its bread dough showed higher springiness and fermentative expansion power. Furthermore, a delay effect of starch retrogradation can be expected compared to the control.

Pediococcus spp.-fermented chicken meat for dogs

  • Lee, Eunchae;Nam, Ki-Taek;Lee, Kyung-Woo;Lee, Sang-Rak
    • Journal of Animal Science and Technology
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    • v.62 no.1
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    • pp.84-93
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    • 2020
  • An experiment was conducted to evaluate Pediococcus spp.-fermented chicken meat as a snack for dogs. The fermented or non-fermented snacks used in this study were prepared through the following process; meat mixtures containing 52.8% MDCM, 35.2% chicken breast meat (CBM) and 9.7% corn starch were inoculated with or without Pediococcus spp., incubated at 37℃ for 24 h and then sterilized at 121℃ for 20 min. During the 24-h fermentation, the pH of fermented chicken snack dropped rapidly with concomitant increase in number of lactic acid bacteria. The nutritional composition was not altered by fermentation. In vitro pepsin nitrogen digestibility was higher (p < 0.05) in the fermented snack compared with the non-fermented snack. Upon storage at room temperature for 14 days, bacteria grew slowly in fermented vs. non-fermented snack samples. In a palatability trial, dogs preferred non-fermented over fermented snack food. In 12-d-long feeding trial, fecal ammonia content was lowered, but fecal lactic acid content was increased in dogs fed the fermented vs. non-fermented snack food. Our study shows that the fermented MDCM-based snack exhibited good preservability upon storage, and improved in vitro nitrogen digestibility and fecal characteristics in dogs.

Quality Characteristics of Pan Bread with Nurungji Powder (누룽지 분말을 첨가한 식빵의 품질 특성)

  • Choi, Ik-Jun;Kim, Do-Yeon;Chung, Chang-Ho
    • Culinary science and hospitality research
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    • v.23 no.7
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    • pp.159-166
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    • 2017
  • This study was to investigate the quality characteristics of white pan bread with added Nurungji, scorched rice. Nurungji powder at the level of 5% (N5), 10% (N10), 15% (N15), 20% (N20) was mixed with flour to make the bread. The overall effects of nurungji powder in pan bread were examined by investigating fermentation rates, volume change, farinograph and TPA analysis, color and preference evaluation. A control showed the highest fermentation rate by 60 min, but there was no significant difference between all samples. Farinograph analysis showed that the water absorption rate was the lowest in control and increased with the addition of nurungji powder. In contrast, in dough development time, control was the shortest and the N20 was the longest. Dough stability showed the best with control, N20 showed the lowest value, and Dough weakness increased significantly as the amount of rice powder was increased. According to TPA analysis it had shown significant differences in hardness and chewiness in the bread with Nurungi. Volume and specific volume tended to decrease as the amount of rice powder added increased. Sensory evaluation showed that the texture of bread had the highest with control, and the overall preference such as color, taste, and flavor showed the best with N15.