• Title/Summary/Keyword: starch granule

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Physicochemical and pasting properties of rice starches from soft rice varieties developed by endosperm mutation breeding (배유 돌연변이처리로 개발된 연질미 전분의 이화학적 특성)

  • Kim, Jae Suk;No, Junhee;Shin, Malshick
    • Korean Journal of Food Science and Technology
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    • v.51 no.2
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    • pp.109-113
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    • 2019
  • The soft rice varieties, Hangaru and Singil, were developed via mutation breeding using N-methyl-N-nitrosourea treatment to obtain dry-milled rice flours. The physicochemical, morphological, and pasting properties of these starches were compared with those of Seolgaeng and Chuchung starches. Singil starch was found to exhibit the highest amylose content and initial pasting temperature, whereas Hangaru starch exhibited the highest water binding capacity and swelling power. Hangaru starch's granule size at $d_{50}$ was the largest among the four different starch types. Some Seolgaeng, Hangaru, and Singil granules were observed to have a round-faced polygon shape. Furthermore, the crystallinity of all four starch types was type A. The peak, trough, and final viscosities of the soft rice starches were also lower than those of normal starches. Notably, Hangaru starch showed the highest breakdown viscosity, but the lowest total setback viscosity among the four starches. From these results, the starch characteristics of the soft rice flours were discovered to be different based on the rice variety.

Changes in Soluble Protein, Free Amino Acid and Starch of Jeungpyun Dough during Fermentation (발효과정 중 증편반죽의 가용성 단백질, 유리아미노산 및 전분의 변화)

  • 박영선;서정식
    • Korean journal of food and cookery science
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    • v.11 no.3
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    • pp.282-286
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    • 1995
  • Physicochemical properties of Jeungpyun dough were analyzed during fermentation in the ranges of 0 to 10 hours. Solube Protein and total content of free amino acid of Jeungpyun dough were decreased at the early stage of fermentation, recording minimum value, 0.292 mg/g-dry matter and 13.31 mg/100g-dry matter at fermentation time, 2 hours and 4 hours respectively while they were increased since then. It was observed that, although few changes occurred at the early stage of fermentation, the height of peak on X-ray diffractogram was decreased somewhat and some disintegration of starch granule on scanning electron micrograph occurred slightly in dough samples of above 6 hours of fermentation time.

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Analysis of Kernel Hardness of Korean Wheat Cultivars

  • Hong, Byung-Hee;Park, Chul-Soo
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.44 no.1
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    • pp.78-85
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    • 1999
  • To investigate kernel hardness, a compression test which is widely used to measure the hardness of individual kernels as a physical testing method was made simultaneously with the measurement of friabilin (15KDa) which is strongly associated with kernel hardness and was recently developed as a biochemical marker for evaluating kernel hardness in 79 Korean wheat varieties and experimental lines. With the scattered diagram based on the principal component analysis from the parameters of the compression test, 79 Korean wheat varieties were classified into three groups based on the principal component analysis. Since conventional methods required large amount of flour samples for analysis of friabilin due to the relatively small amount of friabilin in wheat kernels, those methods had limitations for quality prediction in wheat breeding programs. An extraction of friabilin from the starch of a single kernel through cesium chloride gradient centrifugation was successful in this experiment. Among 79 Korean wheat varieties and experimental lines 50 lines (63.3%) exhibited a friabilin band and 29 lines (36.7%) did not show a friabilin band. In this study, lines that contained high maximum force and the lower ratio of minimum force to maximum force showed the absence of the friabilin band. Identification of friabilin, which is the product of a major gene, could be applied in the screening procedures of kernel hardness. The single kernel analysis system for friabilin was found to be an easy, simple and effective screening method for early generation materials in a wheat breeding program for quality improvement.

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Physicochemical properties of large and small granules of naked barley starches (쌀보리 전분의 입자 크기별 이화학적 성질)

  • Oh, Keum-Soon;Kang, Kil-Jin;Kim, Kwan;Kim, Sung-Kon
    • Applied Biological Chemistry
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    • v.35 no.1
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    • pp.10-13
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    • 1992
  • Starches from three varieties of naked barley were fractionated into two populations according to the granule sizes and physicochemical properties were compared. Number of small granules was comprised in 62% of Saessalbori and 75% of Nulssalbori and Mudungssalbori. The proximate composition and water binding capacity were lower in large granules, the large granules showed higher values in blue value and intrinsic viscosity than those of small ones. There no differences in swelling power and X-ray diffraction pattern between small and large granules. The viscosities of alkali-gelatinized starch were higher on large granules, but the temperature of birefringennce loss was not different.

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Haplotyping and Evolutionary Studies on GBSSII Gene in Korean Rice Collection

  • May Htet Аung;Yong-Jin Park
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2022.10a
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    • pp.218-218
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    • 2022
  • Granules-bound starch synthase II (GBSSII), one of the isoforms of granule-bound starch synthase (GBSS), is responsible for amylose synthesis by expressing in non-storage tissues such as leaf, stem, root, and pericarp. Up to date, little is known about this gene functions and basic knowledge of heritable characteristics of this gene, GBSSII. We identified functional haplotypes and performed evolutionary analyses on the GBSSII using 374 rice accessions (320 Korean bred and 54 wild) based on the classified groups. A total of 14 haplotypes were found, and almost all haplotypes (13) were functional, carrying 19 non-synonymous SNPs in two exons (exons 1 and 2). The lowest nucleotide diversity was detected in Tropical japonica (0.00145), while the highest pi-value was in Aus (0.01081), illustrating the signal of this gene evolution. The highest Tajima's D value in Aus (1.6380) indicates GBSSII gene domestication signature under balancing selection, while the lowest Tajima's D value in Temperate japonica (-0.8243) highlights that they were under positive selection, which may be purified due to the excess of rare alleles. The highest genetic differentiation was observed between Tropical japonica and aroma (FST = 0.921928). In contrast, the highest interbreed level was detected in Aus-admixture (FST = -0.20157). The genetic relatedness between and or among the wild and cultivated subpopulations was revealed through PCA, population structure, and phylogenetic analyses.

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Optimization of ${\beta}$-Glucanase-assisted Extraction of Starch from Domestic Waxy Barley and Its Physicochemical Properties (${\beta}$-Glucanase를 이용한 국내산 찰보리 전분 추출공정의 최적화 및 추출 전분의 주요 이화학적 특성에 관한 연구)

  • Jeong, Yong-Seon;Bae, Jae-Seok;Kim, Jeong-Won;Lee, Eui-Seok;Lee, Ki-Teak;Lee, Mi-Ja;Hong, Soon-Taek
    • Journal of the East Asian Society of Dietary Life
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    • v.23 no.6
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    • pp.789-798
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    • 2013
  • In the present study, optimization on ${\beta}$-glucanase-assisted extraction was made in order to isolate waxy barley starch from domestic cultivar using the D-optimal design suitable for response surface methodology (RSM). The results demonstrated that the amount of enzyme was found to be a major influencing factor on the extraction yield, which was substantially increased by increasing the amount of enzyme. It was also influenced by the reaction time and amount of water addition; however, the two factors were less influential than the amount of enzyme. The optimized condition by RSM for the reaction time was found to be 2.63 hours and amount of enzyme 1.7%, and amount of water addition 4.38 times the weight of raw material. With the enzyme treatment, the starch content in residues (R), particularly in R1 and R5, was reduced considerably, resulting in an increase in the extraction yield and therefore primarily and effectively releasing B-type starch small granule confirmed by scanning electronic microscopy. In addition, the study determined the physicochemical properties of isolated waxy starch (i.e., purity, water adsorption capacity, thermal properties, rheology and starch morphology) and compared them with those from the enzyme-not treated sample. It was found that they were almost similar to each other, except for the purity of starch, which was lower in the enzyme-treated sample than in the enzyme-not treated one.

Properties of Starch Isolated from Wet-milled Rice after Steeping at Elevated Temperatures for Annealing Effect (Annealing 수침처리에 따른 습식제분 쌀가루의 전분 특성)

  • Lee, Young-Tack;Yoo, Moon-Sik;Lee, Bo-Ram;Park, Jong-Hyun;Chang, Hak-Gil
    • Korean Journal of Food Science and Technology
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    • v.36 no.3
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    • pp.393-397
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    • 2004
  • Rice was steeped at elevated temperatures of $40,\;50,\;or\;60^{\circ}C$ for 2 hr, and physicochemical properties of starches isolated from wet-milled rice flour were investigated. Steeping at elevated temperatures slightly decreased lightness of rice starch, while increased yellowness and redness. Average granule size of rice starch was decreased by steeping treatment. Swelling power and solubility increased as temperature increased. Starch from rice steeped at $40^{\circ}C$ displayed highest swelling power and solubility. Differential scanning calorimetry data of starch obtained from steeped rice at $60^{\circ}C$ showed increased onset and peak temperatures, with narrower gelatinization temperature range, suggesting partial annealing effect. Pasting properties of starch measured by Rapid Visco-Analyzer indicated increased peak viscosity due to rice steeping at $40^{\circ}C$. Peak and breakdown viscosities decreased at $50\;and\;60^{\circ}C$, whereas setback and final viscosities increased.

Physicochemical Properties of Waxy Rice Starches Prepared from Three Different Cultivars (다수계 찰벼와 일반찰벼 전분의 이화학적 성질)

  • Kim, Hyong-Soo;Kang, Ock-Joo;Yoon, Gae-Soon
    • Applied Biological Chemistry
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    • v.26 no.4
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    • pp.211-216
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    • 1983
  • The physicochemical properties of starches isolated from three kinds of waxy ricer were investigated. The average diammeter of Orchal starch granule was 3.6u, and those of Hangang and Baegoon starch granules were 4.8u and 5.6u, respectively. X-ray diffraction patterns of all samples were A types. Optical transmittance of starch suspensions was increased rapidly $60{\sim}62^{\circ}C$, $50{\sim}55^{\circ}C$ and $50{\sim}60^{\circ}C$ in Olchal, Hangang and Baegoon, respectively, and all of them exhibited maximum transmittance $75{\sim}80^{\circ}C$ in range. Initial gelationization temperature by means of amylogram pattern was $60{\sim}61^{\circ}C$, then, Olchal starch had a little higher temperature than others. Raising power of them was $260\sim220$, and then, Olchal starch has a little higher raising power. But, water binding capacity of Baegoon and Hangang were somewhat higher than that of Olchal. Blue values of Olchal and Hangang were similar with 0.13, and that of Baegoon was 0. 14. Alkari numbers of Olchal, Hangang and Baegoon were 4.2, 4.9 and 5.1. The degree of retrogradation of Hangang and Baegoon starch gels were somewhat higher than that of Olchal starch gel.

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Extraction of Starch from Domestic Potato Sludge by Food-Grade Hemicellulase and its Physicochemical Properties (식품용 Hemicellulase 계열 효소를 이용한 국내산 감자 가공 부산물의 전분 추출 및 이화학적 특성)

  • Choi, Jung-Min;Park, Cheon-Seok;Baik, Moo-Yeol;Kim, Hyun-Seok;Seo, Dong-Ho
    • Food Engineering Progress
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    • v.21 no.3
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    • pp.280-285
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    • 2017
  • The objective of this study was to increase the efficiency of starch extraction from potato sludge by different concentration of food-grade hemicellulase. The potato sludge, which is a by-product of potato processing industry, was treated with food-grade hemicellulase. Starch extraction efficiency displayed no significant difference in hemicellulase concentration. The purities of potato starch increased from 83.40 to 95.91, 97.44, 95.58, and 97.79%, with treated 0.5, 0.75, 1.0, and 1.5% hemicellulase, respectively. The physicochemical properties of the starches, such as granule structure, particle size, pasting, and thermal transition, were not affected by the concentration of hemicellulase. These results indicate that food-grade hemicellulase treatment is an efficient method for starch extraction from potato sludge.

Physicochemical Properties and Gel Forming Properties of Mungbean and Buckwheat Crude Starches (녹두와 메밀 조전분의 이화학적 특성 및 겔 형성)

  • 주난영;이혜수
    • Korean journal of food and cookery science
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    • v.5 no.2
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    • pp.1-8
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    • 1989
  • The physicochemical properties and gel forming properties of mungbean and buckwheat crude starches were investigated. The results were as follows; 1. The granule size and shape of mungbean crude starch were $11~32\mu\textrm{m}$ and oval, and those of buckwheat crude starch were $3~10 \mu\textrm{m}$ and polygonal. 2. The amylose conteut of mungbean crude starch and buckwheat crude starch were 78.0% and 26.4% respectively. 3. The blue value of mungbean crude starch and buckwheat crude starch were 1.030 and 0.409, respectively. 4. Periodate oxidation of mungbean crude starch resulted that amylose had the average molecular weight of 95, 648, degree of polymerization of 590 and amylopectin had the degree of branching of 5.4, glucose unit per segment of 18.6, and periodate oxidation of buckwheat crude starch resulted that amylose had the average molecular weight of 133, 690, degree of polymerization of 825 and amylopectin had the degree of branching of 5.2, glucose unit per segment of 19.2 5. Water binding capacity of mungbean crude starch was 185.1% and that of buckwheat was 209.9% 6. The pattern of change in swelling power of mungbean crude starch for increasing temperature started to increase at $60^{\circ}C$ and increased rapidly from $70^{\circ}C$, and that of buckwheat increased slowly from $60^{\circ}C$ to $90^{\circ}C$ without rapid increase. 7 The ranges of gelatinization temp. of mungbean and buckwheat crude starches were 63. 9-$109^{\circ}C$ and 52.5-84.$2^{\circ}C$, respectively. 8. The gelatinization patterns for 6% munbean crude starch and 8% buckwheat crude starch were investigated by Brabender amglograph. Mungbean crude starch showed the initial pasting temperature of 77.6$^{\circ}C$ without peak height, and buckwheat crude starch showed that of $62.5^{\circ}C$ without peak height. In addition, sensory evaluation for sample starch gels (mungbean, buckwheat, cowpea) was done. 1. The difference of sensory characteristics for each starch gel was significant. 2. The sample starch gels were regarded as 'Mook' by pannels. 3. 74.44% of the degree of Mooklike was explained by hardness.

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