• Journal of Microbiology and Biotechnology
• /
• 제18권3호
• /
• pp.457-464
• /
• 2008

An extracellular enzyme (RMEBE) possessing ${\alpha}-(1{\rightarrow}4)-(1{\rightarrow}6)$-transferring activity was purified to homogeneity from Rhodothermus marin us by combination of ammonium sulfate precipitation, Q-Sepharose ion-exchange, and Superdex-200 gel filtration chromatographies, and preparative native polyacrylamide gel electrophoresis. The purified enzyme had an optimum pH of 6.0 and was highly thermostable with a maximal activity at $80^{\circ}C$. Its half-life was determined to be 73.7 and 16.7 min at 80 and $85^{\circ}C$, respectively. The enzyme was also halophilic and highly halotolerant up to about 2M NaCl, with a maximal activity at 0.5M. The substrate specificity of RMEBE suggested that it possesses partial characteristics of both glucan branching enzyme and neopullulanase. RMEBE clearly produced branched glucans from amylose, with partial ${\alpha}-(1{\rightarrow}4)$-hydrolysis of amylose and starch. At the same time, it hydrolyzed pullulan partly to panose, and exhibited ${\alpha}-(1{\rightarrow}4)-(1{\rightarrow}6)$-transferase activity for small maltooligosaccharides, producing disproportionated ${\alpha}-(1{\rightarrow}6)$-branched maltooligosaccharides. The enzyme preferred maltopentaose and maltohexaose to smaller maltooligosaccharides for production of longer branched products. Thus, the results suggest that RMEBE might be applied for production of branched oligosaccharides from small maltodextrins at high temperature or even at high salinity.

• 한국식품과학회지
• /
• 제36권4호
• /
• pp.598-603
• /
• 2004

산지가 다른 지역(대만, 중국, 한국, 미국)에서 자란 4종의 메밀전분의 이화학적 특성을 살펴보았다. 그 결과를 살펴보면 전분의 외형은 4종 모두 다각형의 입자로 넓게 분포되어 있으며, X-ray 관찰결과 대다수의 천연 전분과 마찬가지로 4종 모두 A형에 속해 있었다. 수분함량은 6.3-9.58%사이에 있었으며, 조 단백은 대만산과 중국산이 한국산과 미국산시료보다 높게 나타났으며, 지방함량은 한국산과 중국산이 다른 2종의 시료보다 높게 나타냈다. 청가(BV)를 살펴보면 중국산이 0.39로 가장 높게 나타났고, 한국산이 0.32로 가장 낮게 나타났다. 아밀로오스 함량은 미국산이 27.6으로 가장 높고, 한국산이 가장 낮았다. 물결합력에서는 대만산의 시료가 가장 높고, 미국산이 가장 낮았다. 아밀로오스 함량이 비교적 높은 중국산과 미국산은 팽윤력과 용해도에서 낮게 나타났다. 신속점도측정기(RVA)에 의한 측정결과 최고점도, 가공의 안정성을 나타내는 breakdown, 노화에 밀접한 관계를 나타내는 setback 모두 미국산이 가장 높게 나타나 미국산이 비교적 hard gel한 경향을 나타내었다.

• 한국수산과학회지
• /
• 제27권3호
• /
• pp.306-313
• /
• 1994

일본에서 상업적으로 중요한 어종 중의 하나인 도다리 2형간 유전적 분기의 정도를 측정하기 위하여 전분 gel 전기영동법에 의한 isozyme분석을 행하였다. 도다리 2형간에 있어서 14종의 효소를 지배하는 22유전자좌가 검출되었으며 2형간에 있어서 공통의 대림유전자를 가지고 있지 않은 완전분기는 3유전자좌(Acp, Idh-2 및 Mdh-2)에서 관찰되었다. 2형간에 있어서 측정된 Nei의 유전적거리는 0.46592이었으며 2형간에 있어서 유전적 변이성의 차이를 나타내는 평균 이형 접합체율의 기대치는 A type에서 0.120, B type에서는 0.095로 나타났다. 이상의 결과로부터 2형간에 있어서 유전적으로 상호 분기되어 있었으며 그들의 분기 년도는 대략 $2.3{\times}10^6$년 전으로 추정된다.

• Asian-Australasian Journal of Animal Sciences
• /
• 제8권2호
• /
• pp.187-194
• /
• 1995

Genetic variants of ${\alpha}s_1$-casein, ${\beta}$-casein, ${\kappa}$-casein and ${\beta}$-lactoglobulin were investigated by starch urea gel electrophoresis in milk samples of 280 Korean native cattle. A new ${\beta}$-casein variant, designated ${\beta}$-casein $A^4$, was found in milk samples of Korean native cattle. It has a much slower electrophoretic mobility than the ${\beta}$-casein $A^3$ variant in acid gel. This new variant appeared together with either ${\beta}$-casein $A^1$, $A^2$ or B variant. Gene frequencies and genotypic frequencies were estimated. Gene frequencies of four milk protein loci in Korean native cattle were compared with those of imported cattle breeds raised in Korea and Japanese brown cattle. Gene frequencies were ${\alpha}s_1$-casein B .846, ${\alpha}s_1$-casein C .154; ${\beta}$-casein $A^1$ .216, ${\beta}$-casein $A^2$ .666, ${\beta}$-casein $A^4$ .048, ${\beta}$-casein B .070; ${\kappa}$-casein A .648, ${\kappa}$-casein B .352; ${\beta}$-lactoglobulin A .148, ${\beta}$-lactoglobulin B .852. The population was in Hardy-Weinberg equilibrium at all milk protein loci. Gene frequencies of Korean native cattle were very similar to those of Japanese brown cattle. Interestingly, a new variant, ${\beta}$-casein $A^4$, was found only in Korean native cattle and Japanese brown cattle. These results support the hypothesis that Korean native cattle were used in the development of the Japanese brown cattle.

• Journal of Plant Biology
• /
• 제17권4호
• /
• pp.143-156
• /
• 1974

The purpose of this experiment was to study one side of germination physiology based on that protein profiles and protease relating to protein metabolism, that peroxidase, catalase, $\alpha$-amylase, $\beta$-amylase, and malate dehydrogenase involved in the carbohydrate metabolism of seed germination. All these experiments were divided into the two groups with and without acetone treatment, and were carried out. The protein bands of each germinating stage between the groups treated with and without acetone showed certain basic pattern in polyacrylamide gel disc electrophoresis. However, there was a little difference in the number of protein band, optical density, and migration velocity between two groups. The isozyme bands of peroxidase, and catalase between two groups in polyacrylamide gel disc electrophoresis did not show the numeral difference, but the optical density of certain germinating stage treated with acetone was higher than the group untreated with it and it showed their enzyme activity. The $\alpha$-amylase and $\beta$-amylase activities which involved in starch metabolism of seed germination were higher in the treated group than the other. On one hand, the protease activity of hydrolase occurred in the seeds for germination was also higher, more or less in the treated group than in the other. The isozyme band pattern of malate dehydrogenase in TCA cycle of energy metabolism pathway was very different between two groups growing for 72 hours with and without acetone treatment in cellulose acetate electrophoresis. It indicated that two isozyme bands of malate dehydrogenase was high. Consequently these experimental results mentioned above indicated that acetone treatment before sowing had an effect on dissolving certain complexed lipid substance involved in the seed coats, the activity of carbohydrate hydrolase increased with water absorption which was most comfortable in its germination, dissolved glycerin and fatty acid became certain energy source, and they stimulated the acceleration of respiration metabolism.

• 대한수의학회지
• /
• 제28권2호
• /
• pp.299-305
• /
• 1988

The phenotypes of hemoglobin, albumin and transferrin of 3U2 Jindo dogs in Jindo area were studied by starch gel electrophoresis for hemoglobin and albumin, and by polyacrylamide gradient gel electrophoresis for transferrin. The results obtained were as follows: 1. In the hemoglobin phenotypes, three phenotypes, HbAA, HbAB and HbBB, which were controlled by two allelic genes, $Hb^A$ and $Hb^B$, were observed and their frequencies of appearance were 1.65%, 10.60% and 87.75% respectively. The distribution of gene frequency was calculated as 0.0695 in $Hb^A$ and 0.9305 in $Hb^B$. 2. In the albumin phenotypes, three phenotypes, Alb FF, Alb FS and Alb SS, which were controlled by two allelic genes, $AIb^F$ and $AIb^S$ were observed and their frequencies of appearance were 12.59%, 25.56% and 61.85% respectively. The distribution of gene frequency was calculated as 0.2537 in $AIb^F$ and 0.7463 in $AIb^S$. 3. Analysis of transferrin phenotypes showed 6 different types which were controlled by three allelic genes, $Tf^B$, $Tf^C$ and $Tf^D$ and their frequencies of appearance were 54.04% in TfBB, 17.54% in TfBC, 9.82% in TfBD, 8.07% in TfCC, 7.37% in TfCD and 3.16% in TfDD. The distribution of gene frequency was calculated as 0.6772 in $Tf^B$, 0.2053 in $Tf^C$ and 0.1175 in $Tf^D$.

• Geomechanics and Engineering
• /
• 제33권1호
• /
• pp.29-40
• /
• 2023

Xanthan gum and starch compound biopolymer (XS), an environmentally friendly soil-binding material produced from natural resources, has been suggested as a slope protection material to enhance soil strength and erosion resistance. Insufficient wet strength and the consequent durability concerns remain, despite XS biopolymer-soil treatment showing high strength and erosion resistance in the dried state, even with a small dosage of soil mass. These concerns need to be solved to improve the field applicability and post-stability of this treatment. This study explored the utilization of an alkaline-based cation crosslinking method using calcium hydroxide and sodium hydroxide to induce non-thermal gelation, resulting in the enhancement of the wet strength and durability of biopolymer-treated soil. Laboratory experiments were conducted to assess the unconfined compressive strength and cyclic wetting-drying durability performance of the treated soil using a selected recipe based on a preliminary gel formation test. The results demonstrated that the uniformity of the gel structure and gelling time varied depending on the ratio of crosslinkers to biopolymer; consequently, the strength of the soil was affected. Subsequently, site soil treated with the recipe, which showed the best performance in indoor assessment, was implemented on the field slope at the bridge abutment via compaction and pressurized spraying methods to assess feasibility in field implementation. Moreover, the variation in surface soil hardness was monitored periodically for one year. Both slopes implemented by the two construction methods showed sufficient stability against detachment and scouring, with a higher soil hardness index than the natural slope for a year.

• 한국식품과학회지
• /
• 제25권5호
• /
• pp.546-551
• /
• 1993

제분방법별(Pin mill, Colloid mill, Micro mill, Jet mill)로 제조한 쌀가루의 특성과 쌀 수제비 제조후 식미에 미치는 영향을 조사하였다. 지방, 회분, 단백질은 제분 방법에 따라 큰 차이를 보이지 않았다. 제분방법별 쌀 가루의 청값, 열수가용성 아밀로오스 함량, 그리고 전분 손상도는 Jet mill, Micro mill, Colloid mill, Pin mill의 순으로 높았음을 나타내었다. 수분흡수지수, 수분용해지수 및 보수력을 측정한 결과 전분손상도가 증가함에 따라 전체적으로 증가하였다. 또한 시료농도 15%의 겔을 형성하였을 때 Pin mill의 경우 강도에 있어서 가장 높음을 보여주었다. 아밀로그램을 이용한 호화개시온도와 최고점도는 입자가 미세하여 짐에 따라 점차 낮아지는 경향을 보였으며 Differential Scanning Colorimetry 측정에 의한 측정값도 아밀로그램에서와 동일한 양상을 나타내어 호화흡열의 면적에서 산출한 호화엔탈피$({\Delta}H)$가 Jet mill의 경우 0.83 kcal로 가장 낮았다. 즉 전분의 손상도가 증가함에 따라 호화엔탈피는 감소하였다. 쌀수제비 제조실험 결과는 Jet mill이 관능검사 결과 가장 우수한 것으로 판명되었고 반죽상태도 양호함을 보여주었다.

• 한국식품조리과학회지
• /
• 제17권4호
• /
• pp.391-398
• /
• 2001

녹두전분 겔을 온도(5$^{\circ}C$, $25^{\circ}C$) 및 기간(1, 24, 48, 72시간)별로 저장하여 겔의 열적 특성, 점도 특성, 효소소화도, X-선 회절도, 미세구조의 변화를 조사하였다. DSC 실험 결과 5$^{\circ}C$ 저장시 $25^{\circ}C$ 저장시보다 용융피크가 저온측에 있고, 용융엔탈피 값은 더 큰 값을 나타내어 저온에서 겔의 노화가 빠르게 진행됨을 나타내었다. RVA에 의한 점도 측정 결과 $25^{\circ}C$에서 3일간 저장한 시료를 제외하면, 5$^{\circ}C$ 및 $25^{\circ}C$ 저장 모두 peak 점도, 최종점도 등의 점도 특성이 저장에 의해 증가하였는데, 이러한 현상은 5$^{\circ}C$ 저장시 보다 현저하였다. 효소소화도는 저장기간이 경과함에 따라 감소했으며, 이러한 경향은 5$^{\circ}C$ 저장시 더 현저하였다. X-선 회절도는 $25^{\circ}C$ 저장시에는 매우 약한 피크가, 5$^{\circ}C$ 저장에서는 보다 강한 피크가 관찰되었지만, 동일 온도에서 저장한 시료 사이의 차이는 구별하기 어려웠다. 주사전자현미경에 의한 미세구조 관찰 결과 모든 겔에서 잘 발달된 망상구조가 관찰되었으며, $25^{\circ}C$에서 저장시에는 3일째에 전분쇄의 망상구조가 두꺼워졌고, 5$^{\circ}C$ 저장시는 저장 초기부터 망상구조가 두꺼워졌다.

• 농업과학연구
• /
• 제22권1호
• /
• pp.103-109
• /
• 1995

단백질과 전분을 각 성분비로 조합하고, 고형물 함량 8, 9, 10, 11%로 조정한 시료를 $80^{\circ}C$에서 30분간 가열한 후 $25^{\circ}C$로 냉각하여, 온도 $30{\sim}60^{\circ}C$, 0.6 ~ 6 rpm의 범위에서 Brookfield 단원통회전점도계로 리올로지 특성을 측정하였는 바, 아래와 같은 결과를 얻었다. 1. 모델 식품 $P_1S_3$, $P_1S_2$, $P_1S_1$, $P_2S_1$, $P_3S_1$, $P_4S_0$는 모두 의가소성을 나타내고, 항복치를 가지며, 시간 의존성 구조 붕괴를 나타내는 thixotropic 식품이었다. 그러나 $P_0S_4$, 즉 전분의 경우는 8~11% 범위에서 gel의 강도가 크기 때문에 유동성을 보이지 않았다. 2. 각 수분함량에서 모형식품의 단백질 함량에 따른 유통 특성값은 일정한 상관관계를 나타내지 않았다. 3. 전단속도에 대한 전단웅력의 변화는 전분질식품이 ($P_1S_3$, $P_1S_2$) 단백질식품($P_2S_1$, $P_3S_1$)보다 컸으며, 전단초기의 구조붕괴는 Tiu의 모델에 따라 2차 반응식으로 붕괴되었고, 전단속도가 증가 할 수록 구조 붕괴 속도도 빨랐다. 4. $P_1S_2$, $P_2S_1$의 온도의존성은 Arrhenius식에 잘 따랐으며 이때 활성화에너지는 각각 2.35, $1.34Kcal/g{\cdot}mole$이었다.