• The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
• /
• v.12 no.1
• /
• pp.18-35
• /
• 1999

Coptidis Rhizoma, Fraxini Cortex, Jinpisan(秦皮散) have been as eye washes of inflammatory eye disease in the oriental medicine. Especially Jinpisan(秦皮散) has been used for the disease which is similar to Peudomonas aeruginisa keratitis. This research was attempted to investigate the effect of Coptidis Rhizoma, Fraxini Cortex, Jinpisan(秦皮散), on Peudoronas aeruginisa keratitis. Pseudomonas aeruginosa keratitis causes a deep rapid intense ulceration which often leads to perforation of the cornea within 48 hours. In this research, we induced keratits in the rabbits by inoculating Pesudomonas aeruginosa(9027) and observed the effect on the keratitis and the irritation against the external eye. Also we mesured the minimum inhibitory consentration(MIC) of Coptidis Rhizoma, Fraxini Cortex, Jinpisan(秦皮散) by agar diliution method and the anti-bacterial activites by disk method. The tested bacteria were as follows : a) Pseudomonas aeruginosa (9027), b) Streptococcus pneumoniae(6303), c) Staphylococcus epidermidis(12228), d) Staphylococcus aureus(6538P). The results were as follows ; 1. The groups which were applied eye washes of Fraxini Cortex, Jinpisan reavealed a significant effect, but the group applied eye wash of Coptidis Rhizoma reveaded no effect on Pseudomonas aeruginosa keratitis. 2. Applying eye washes of Coptidis Rhizoma, Fraxini Cortex, Jinisan revealed an irritation against external eyes. 3. Coptidis Rhizoma showed an anti-bacterial activity on Pseudomonas aeruginosa, Streptococcus pneumoniae, Staphylococcus epidermidis, Staphylucoccus aureus by agar diliution method 4. Coptidis Rhizoma showed an anti-bacterial activity on Pseudomonas aeruginosa, Streptococcus pneumoniae, Staphylococcus epidermidis, Staphylococcus aureus by disk method. 5. Fraxini Cortex showed an anti-bacterial activity on Pseudomonas aeruginosa, Streptococcus pneumoniae, Staphylococcus epidermidis, Staphylococcus aureus by agar diliution method 6. Fraxini Cortex showed an anti-bacterial activity on Pseudomonas aeruginosa, Sireptococcus pneumoniae, Staphylococcus epidermidis, Staphy1ococcus aureus by disk method. 7. Jinpisan showed an anti-bacterial activity on Pseudomonas aeruginosa, Streptococcus pneumoniae, Staphylococcus epidermidis, Staphylococcus aureus by agar diliution method. 8. Jinpisan showed an anti-bacterial activity on Pseudomonas aeruginosa, Streptococcus pneumoniae, Staphylococcus epidermidis, Staphylococcus aureus by disk method. According to the above results, Fraxini Cortex, Jinpisan(秦皮散) are recognized to have an effective treatment on the Pesudomonas aeruginosa keratitis, so this experiment is thought to be a basic ingredient in proving the effect of Fraxini Cortex, Jinpisan which is applied many in documents and clinical medicine. In the comparison of anti-bacterial activity and results of treatment on the Pesudomonas aeruginosa keratitis, Jinpisan(秦皮散) was more effective than Coptidis Rhizoma, Fraxini Cortex.

• Journal of Food Hygiene and Safety
• /
• v.36 no.6
• /
• pp.488-492
• /
• 2021

One of the well characterized bacteriocins, nisin, shows strong antimicrobial activity against pathogenic bacteria such as Listeria monocytogenes and Staphylococcus aureus. This study evaluated the synergistic effect of commercial nisin and SAP84 bacteriophage on S. aureus. Nisin showed antimicrobial activity against S. aureus KCTC 3881 in a dose-dependent manner. Eighteen IU/mL of the nisin decreased 4.03 Log CFU/mL of the strain in MRS broth after six hours compared with the controlled subject. On the other hand, the same dose of the nisin decreased 5.54 Log CFU/mL when co-treated with 0.1 MOI of the bacteriophage SAP84. Furthermore, the combination of nisin and SAP84 was successfully applied for controlling the S. aureus strain in lettuce.

• Journal of Pharmacopuncture
• /
• v.10 no.3
• /
• pp.47-52
• /
• 2007

Objective This experimental study was performed to investigate the effect of Coptidis rhizoma extract compared with quantity on Staphylococcus aureus that induce keratitis. Methods Minimal inhibitory concentration(MIC) was measured by dropping to $50{\mu}l$　according to density Coptidis rhizoma extract(100%, 10%, 1%, 0.1%) compared with quantity(40g, 80g, 160g). Anti-bacterial potency was measured by the size of inhibition zone with change of volume. Results 1. MIC on Staphylococcus aureus in Coptidis rhizoma extract was showed anti-bacterial potency compared with quantity and density in 100% and 10% of all samples(40g, 80g, 160g). 2. MIC on Staphylococcus aureus in Coptidis rhizoma extract(40g, 80g, 160g) was showed anti-bacterial potency compared with quantity all samples($20{\mu}l,\;30{\mu}l,\;40{\mu}l,\;50{\mu}l$) in 100% density. 3. MIC on Staphylococcus aureus in Coptidis rhizoma extract(40g, 80g, 160g) was showed anti-bacterial potency compared with quantity all samples ($20{\mu}l,\;30{\mu}l,\;40{\mu}l,\;50{\mu}l$) in 100% density. Anti-bacterial potency of 80g Coptidis rhizoma extract decreased compared with 40g. Anti-bacterial potency of 160g Coptidis rhizoma extract decreased compared with 40g in $20{\mu}l,\;30{\mu}l$. Conclusions Coptidis rhizoma extract was showed anti-bacterial potency compare with quantity and density. In herbal drug, antibacterial potency compare with quantity and density must be studied.

• Journal of Life Science
• /
• v.9 no.4
• /
• pp.358-367
• /
• 1999

Thirty strains of methicillin resistant Staphylococcus aureus were obtained from the clinical isolates. In order to investigate the pursuit of the pathogens of nosocomial infection, these strains were studied for antibiotic sensitivity as well as its resistant pattern. Among the methods of hybridization which directly confirm the specific antibiotic resistant genes by means of the recently developed specific probe DNA, dot blot hybridization and southern blot hybridization were performed and these two methods were compared in their sensitivity and specificity. Strains that is sensitive to cephalothin to the subject of methicillin resistant Staphylococcus aureus were in 43%. Those that are sensitive to cefoperazone and cefuroxime were 26% and 23%, respectively. In case of MIC, MIC50 of cefoperazone was 8 $\mu\textrm{g}$/$m\ell$, and MIC90 was 128 $\mu\textrm{g}$/$m\ell$ to be the lowest. As the results of plasmid DNA electrophoresis, most of methicillin resistant Staphylococcus aureus strains had more than 4 plasmids. These plasmids digested by BamHI, methicillin resistant Staphylococcus aureus is distributed as 10 fragments with the size of 65 kb to 1.5 kb. Dot blot hybridization were performed to examine the existence of mecA gene to show the detection rate of 50%. Southern blot hybridization were done to see if DNA bands which amplify the activity of digoxigenium-labeled probe by PCR were actually PCR products of mecA gene and it showed the detection rate of 53%. It can be concluded that the southern blot hybridization seemed to be better in sensitivity and specificity when it is compared with the results of dot blot hybridization.

• Journal of the East Asian Society of Dietary Life
• /
• v.5 no.2
• /
• pp.19-26
• /
• 1995

Staphylococcus aureus, the most salt-tolerant nonhalophilic bacterium, is the only foodborne pathogen that is able to grow at a levels below 0.90. The fundamental osmorgulatory strategy used by this organism involves the accumulation of intracellular compatible solutes such as proline or glycine betaine which are accumulated by transport and act as osmoregulators in cells. In this study, levels of proline transport systems and glycine betaine transport system of S. aureus were examined when cells are grown at high osmolarity. The levels of all three transport systems within S. aureus were elevated at high osmolarity and the most dramatic increase was found for the low-affinity proline transport system. However, in 5mM glycine betaine-supplemented medium, the level of the low-affinity proline transport system did not become elevated when cultures were grown at high osmolarity. The metabolic fate of the accumulated proline and glycine getaine was investigated by thin-layer chromatography an found to be not metabolized by S. aureus.

• Microbiology and Biotechnology Letters
• /
• v.51 no.4
• /
• pp.555-558
• /
• 2023

Staphylococcus aureus is a prominent multidrug-resistant pathogen known for its resistance to a variety of antibiotics. To combat this, a wide range of antibiotics, including quinolones, is utilized. While the efficacy of quinolones against S. aureus has been established, the rise in quinolone-resistant strains, particularly in methicillin-resistant S. aureus (MRSA), has necessitated a shift in their usage patterns. Genomic sequencing plays a crucial role as it offers insights into the genetic mechanisms of resistance. Thus, we report the complete genome sequence of an oxolinic acid-resistant strain of S. aureus isolated from sweet potato leaves, a crop commonly cultivated in Korea.

• Korean Journal of Microbiology
• /
• v.28 no.1
• /
• pp.27-34
• /
• 1990

Two macrolide-lincosamide-streptogramin B (MLS) antibiotic resistance genes, one expressed inducibly and the other expressed constitutively were recognized from a single Staphylococcus aureus DH1 strain. The inducible MLS resistance gene was isolated and cloned from the R-plasmid pDE1(7.4kb) and the constitutive gene was from chromosomal DNA. Base sequence of the inducible MLS resistance gene (1.2kb) was determined and found as same that of pE194. The restriction map of the cloned constitutive MLS resistance gene was compared with that of the inducible gene. Two genes have same restriction map except leader region. In the constitutive gene there is no leader region which is doing major role in inducible expression.

• Fashion & Textile Research Journal
• /
• v.11 no.5
• /
• pp.827-832
• /
• 2009

The purpose of this study is to scientifically proof possibility of development of natural dye which has antimicrobial activity with Chelidonium majus var. asiaticum. For that silk fabrics dyed with Chelidonium majus var. asiaticum extracts were tested for anti-microbial activity. Bacterials used for test of anti-microbial activity were Staphylococcus aureus ATCC 6538, Klebsiella pneumoniae ATCC 4352 and Trichophyton mentagrophytes IFO 5466. The results of experiment were as follows ; 1. The more the number of times of dyeing increase the more value of K/S increased. 2. Reduction of bacterium against Staphylococcus aureus ATCC 6538 of silk fabrics dyed with Chelidonium majus var. asiaticum extracts was 99.9% without reference to the number of times of dyeing. 3. Reduction of bacterium against Staphylococcus aureus ATCC 6538 of silk fabrics dyed with Chelidonium majus var. asiaticum extracts was 99.9% after irradiation of 20 hour without reference to the number of times of dyeing. 4. Reduction of bacterium against Staphylococcus aureus ATCC 6538 of silk fabrics dyed with Chelidonium majus var. asiaticum extracts was over 94.8% after dry cleaning. As above silk fabrics dyed with Chelidonium majus var. asiaticum extracts were acquired a high anti-microbial activity against Staphylococcus aureus ATCC 6538.

• Journal of Microbiology and Biotechnology
• /
• v.7 no.6
• /
• pp.402-408
• /
• 1997

The antibiotic-producing strain HW-003 was screened from soil and found to be effective against the multidrug-resistant Staphylococcus aureus. The spore chain of HW-003 was retinaculiaperti, and the spore surface was spiny. Strain HW-003 has a LL-diaminopimelic acid isoform in the cell wall. The aerial mass color of the strain was gray, and the reverse side was yellow-brown. The strain produced melanin, but did not produce soluble pigments. According to the Taxon program, HW-003 showed best match with Streptomyces cyaneus. Antibiotic production reached a maximum after 72-h cultivation. The antibiotic was purified with silica gel column chromatography, octadecylsilyl column chromatography, and HPLC. The purified antibiotic, AMRSA1, showed strong inhibitory activity against multidrug-resistant Staphylococcus aureus and gram-positive bacteria. The molecular weight of AMRSA1 was about 1, 100. AMRSA1 was a peptide antibiotic containing alanine and serine.

• YAKHAK HOEJI
• /
• v.34 no.2
• /
• pp.122-125
• /
• 1990

Antibiotic resistance patterns were determined for 14 strains of Staphylococcus aureus isolated at a hospital in Pusan during summer in 1989. Resistance to chloramphenicol or clindamycin was recorded in 100% of strains. Resistance to the other compounds tested was as follows: tetracycline 86%, gentamicin 79%, tobramycin 71%, kanamycin 71%, erythromycin 57%, ampicillin 57%, methicillin 50%, streptomycin 29%, cephalothin 29%, and trimethoprim 21%. All strains were sensitive to vancomycin and rifampicin. All strains showed multiple resistance to more than 3 antibiotics.