• Asian Pacific Journal of Cancer Prevention
• /
• v.16 no.9
• /
• pp.3595-3604
• /
• 2015

Hepatocellular carcinoma (HCC) has been one of the most fatal malignant tumors worldwide and its associated morbidity and mortality remain of significant concern. Based on in-depth reviews of serological diagnosis of HCC, in addition to AFP, there are other biomarkers: Lens culinaris agglutinin-reactive AFP (AFP-L3), descarboxyprothrombin (DCP), tyrosine kinase with Ig and eprdermal growth factor (EGF) homology domains 2 (TIE2)-espressing monocytes (TEMs), glypican-3 (GPC3), Golgi protein 73 (GP73), interleukin-6 (IL-6), and squamous cell carcinoma antigen (SCCA) have been proposed as biomarkers for the early detection of HCC. The diagnosis of HCC is primarily based on noninvasive standard imaging methods, such as ultrasound (US), dynamic multiphasic multidetector-row CT (MDCT) and magnetic resonance imaging (MRI). Some experts advocate gadolinium diethyl-enetriamine pentaacetic acid (Gd-EOB-DTPA) MRI and contrast-enhanced US as the promising imaging madalities of choice. With regard to recent advancements in tissue markers, many cuting-edge technologies using genome-wide DNA microarrays, qRT-PCR, and proteomic and inmunostaining studies have been implemented in an attempt to identify markers for early diagnosis of HCC. Only less than half of HCC patients at initial diagnosis are at an early stage treatable with curative options: local ablation, surgical resection, or liver transplant. Transarterial chemoembolization (TACE) is considered the standard of care with palliation for intermediate stage HCC. Recent innovative procedures using drug-eluting-beads and radioembolization using Yttrium-90 may exhibit beneficial effects in HCC treatment. During the past few years, several molecular targeted agents have been evaluated in clinical trials in advanced HCC. Sorafenib is currently the only approved systemic treatment for HCC. It has been approved for the therapy of asymptomatic HCC patients with well-preserved liver function who are not candidates for potentially curative treatments, such as surgical resection or liver transplantation. In the USA, Europe and particularly Japan, hepatitis C virus (HCV) related HCC accounts for most liver cancer, as compared with Asia-Pacific regions, where hepatitis B virus (HBV) may play a more important role in HCC development. HBV vaccination, while a vaccine is not yet available against HCV, has been recognized as a best primary prevention method for HBV-related HCC, although in patients already infected with HBV or HCV, secondary prevention with antiviral therapy is still a reasonable strategy. In addition to HBV and HCV, attention should be paid to other relevant HCC risk factors, including nonalcoholic fatty liver disease due to obesity and diabetes, heavy alcohol consumption, and prolonged aflatoxin exposure. Interestingly, coffee and vitamin K2 have been proven to provide protective effects against HCC. Regarding tertiary prevention of HCC recurrence after surgical resection, addition of antiviral treatment has proven to be a rational strategy.

• 한국환경농학회:학술대회논문집
• /
• 2009.07a
• /
• pp.240-272
• /
• 2009

Recently, as Foot and Mouth disease(FMD) outbreaks in South-East Asia, Taiwan, China, we cannot loose our tense to sustain our FMD free country status. And we have increasing possibility of disease inflow because of continual outbreaks of FMD type A, O and Asia 1 in neighbored countries, foreign visitor and foreign workers. So we have to be urgently ready for strengthen surveillance, early-report and early-diagnosis. So the most important things in epidemic control of livestock are co-work between central and local epidemic control office and field epidemic control. However, potential risk for FMD recurrence and sporadic occurrence of epidemic disease still threaten our livestock farmhouse, so we have to be ready for bio-security against these threatening. For these reasons, I will introduce the concept, etiology and epidemiology of disease and investment/ analysis of health management standard of main animals like cattle and pig which is applied to the inside and outside of country. With these references, we have to manage livestock health management thoroughly by establishing livestock health management standard and notifying special veterinarian and livestock farmer of these standard. Later on, prevention and quarantine of epidemic outbreak and establishing livestock health management standard should be undertaken primarily for sustainable growth and stability of livestock farm industry.

• Journal of Physiology & Pathology in Korean Medicine
• /
• v.22 no.1
• /
• pp.262-266
• /
• 2008

We plan to make the standardization of the pattern identifications for stroke and differentiate them by tongue diagnosis. We make a case report form which has questionnaires for tongue diagnosis in stroke patients. And we collected cases from the multi center network which consists of twelve university hospitals and one local hospital. The cases confirmed by diagnosis of medical specialists and residents are 321 cases. They are divided into Qi Defficiency 30.84%, Dampness& Phlegm 25.55%, Fire & Heat 22.43%, Eum Defficiency 18.69% and Blood Stasis 2.49%. We analyzed the markers which classified into the color of tongue body, the color of fur, the quality of fur, the dryness of tongue, the shape of tongue. To make a stroke pattern identification standard, we must try variable ways.

• Imaging Science in Dentistry
• /
• v.50 no.2
• /
• pp.153-159
• /
• 2020

Purpose: This study was conducted evaluate the influence of reconstruction parameters of micro-computed tomography (micro-CT) images on bone mineral density (BMD) analyses. Materials and Methods: The sample consisted of micro-CT images of the maxillae of 5 Wistar rats, acquired using a SkyScan 1174 unit (Bruker, Kontich, Belgium). Each acquisition was reconstructed following the manufacturer's recommendations(standard protocol; SP) for the application of artifact correction tools(beam hardening correction [BHC], 45%; smoothing filter, degree 2; and ring artifact correction [RAC], level 5). Additionally, images were reconstructed with 36 protocols combining different settings of artifact correction tools (P0 to P35). BMD analysis was performed for each reconstructed image. The BMD values obtained for each protocol were compared to those obtained using the SP through repeated-measures analysis of variance with the Dunnett post hoc test(α=0.05). Results: The BMD values obtained from all protocols that used a BHC of 45% did not significantly differ from those obtained using the SP (P>0.05). The other protocols all yielded significantly different BMD values from the SP(P<0.05). The smoothing and RAC tools did not affect BMD values. Conclusion: BMD values measured on micro-CT images were influenced by the BHC level. Higher levels of BHC induced higher values of BMD.

• Genomics & Informatics
• /
• v.21 no.4
• /
• pp.52.1-52.10
• /
• 2023

Accurate and efficient microbial diagnosis is crucial for effective molecular diagnostics, especially in the field of human healthcare. The gold standard equipment widely employed for detecting specific microorganisms in molecular diagnosis is quantitative real-time polymerase chain reaction (qRT-PCR). However, its limitations in low metagenomic DNA yield samples necessitate exploring alternative approaches. Digital PCR, by quantifying the number of copies of the target sequence, provides absolute quantification results for the bacterial strain. In this study, we compared the diagnostic efficiency of qRT-PCR and digital PCR in detecting a particular bacterial strain (Staphylococcus aureus), focusing on skin-derived DNA samples. Experimentally, specific primer for S. aureus were designed at transcription elongation factor (greA) gene and the target amplicon were cloned and sequenced to validate efficiency of specificity to the greA gene of S. aureus. To quantify the absolute amount of microorganisms present on the skin, the variable region 5 (V5) of the 16S rRNA gene was used, and primers for S. aureus identification were used to relative their amount in the subject's skin. The findings demonstrate the absolute convenience and efficiency of digital PCR in microbial diagnostics. We suggest that the high sensitivity and precise quantification provided by digital PCR could be a promising tool for detecting specific microorganisms, especially in skin-derived DNA samples with low metagenomic DNA yields, and that further research and implementation is needed to improve medical practice and diagnosis.

• Journal of Society of Preventive Korean Medicine
• /
• v.20 no.2
• /
• pp.17-26
• /
• 2016

Objectives : We presented the results of reliability study in advance, and analyzed agreement between Korean medicine doctors(KMDs)' diagnosis and cold-heat pattern identification questionnaire(CHPI)'s diagnosis. Methods : This survey was conducted from May 16 to 17, 2015. The subjects were 93 adults living in rural society. Diagnosis of CHPI was performed by 2 KMDs who have clinical experience more than 5 years. The KMDs' diagnosis was set as a reference index, and then we compare 23 items(cold pattern 11 items and heat pattern 12 items) of CHPI questionnaire and 15 items(cold pattern 8 items and heat pattern 7 items) that were brief form of it. We had cut-off value by standard of KMDs' diagnosis using receiver operating characteristic-curve(ROC-curve), with which we calculated agreement including kappa value. Correlation analysis between CHPI evaluation score by KMDs and by the questionnaire was fulfilled as well. Results : Agreement about 11 and 8 cold pattern items showed 87.1% together, and the value of kappa each recorded 0.742 and 0.741. Agreement about 12 and 7 heat pattern items suggested 81.7% and 78.5%, and the value of kappa showed 0.634 and 0.570. Correlation coefficients were 0.803 of 11 items and 0.761 of 8 items about cold pattern. In addition, correlation coefficients were 0.789 of 12 items and 0.767 of 7 items about heat pattern. The significant probability (p-value) was under 0.001. Conclusions : We have developed CHPI questionnaire involving reliability and agreement based on usual symptoms, and hope additional complements so that Korean medicine diagnostics and Korean preventive medicine would be improved.

• Journal of Sasang Constitutional Medicine
• /
• v.24 no.3
• /
• pp.34-41
• /
• 2012

Objectives In this study, we developed a mock-up of the system for Sasang Constitutional (SC) diagnosis. This system consists of 5 devices which are the face analyzer, the voice analyzer, the skin analyzer, the pulse analyzer, and the computer-based questionnaire. Our goal is to evaluate the repeatability of the system. Methods Each device is capable of classifying SC types. The classification probability of the integrated system for the SC types was obtained by summing the probability from each device. For evaluating the repeatability of the system, we collected data for 5 subjects, and repeated the measurement three times for each individual. The average and standard deviation were used for calculating the Coefficient of Variation. Results The results showed that the repeatability of the classification probability of the integrated system is about 8%, which implies the system is repeatable. Conclusions To increase usability of this system, it is desirable for the system to offer information on health condition of the user. The integrated constitutional diagnosis system will be upgraded to complement the convenience and to develop the diagnostic algorithm for the user's health condition.

• Proceedings of the KIEE Conference
• /
• 2008.07a
• /
• pp.1997-1998
• /
• 2008

Pulse diagnosis is the one of the most important diagnostic process to traditional medical doctors. Although the pulse diagnosis position, Gwan is apart from Chon or Cheok by 10$\sim$20mm at most, traditional medical doctors applies different indent pressures and even they states different pulse images are felt at Chon, Gwan and Cheok. One the other hand, the education on pulse diagnosis behavior includes tantalizing problem caused by no tool for communication between trainer and trainee. On account of this situation, we tried to develop a system which can measure the hold-down pressure during a pulse diagnosis and compare the hold-down pressure profile of trainer and that of trainee. This system can be divided into three parts - pulse pressure sensing part, signal acquisition part and data storing part. A correction curve was generated by the relation between output voltages and standard weights. Using this system, 3 channel hold-down pressure profile of a oriental medical doctor were recorded three times. In the profile, three period were observed and all period included two process for searching the depth of pulsation and for classifying the pulse feeling into one or more of 28 pulse types. The maximum value of pulse profile was 1.3$kg{\cdot}f$ which was more than reported by previous chinese groups and the mean values of three channel ranged from 240$g{\cdot}f$ to 430$g{\cdot}f$. In frequency domain, each channel has some dominant frequency components - about 10Hz, 35Hz and 75Hz. In further study, we want to collect more profiles from lage number of oriental medicine doctors and hope to develop a measuring system which can measure the hold-down pressure on subject's skin directly.

• The Journal of Korean Medicine
• /
• v.43 no.1
• /
• pp.1-5
• /
• 2022

Objectives: Using algometer, measure the pressure pain threshold (PPT) of the epigastric pain(心下痞硬) and calculate the cut-off value, and this can serve as the basis for prognostic diagnosis of functional dyspepsia so we would like to evaluate its diagnostic value. Methods: We investigated 353 patients with functional dyspepsia symptoms who admitted Gangnam Weedahm Oriental Hospital from February 1, 2021 to February 27, 2021. At the time of the patient's visit, an oriental medical doctor measured the pressure at the first pain point on the Algometer of (CV14), twice each, at 1minute intervals. The ROC (receiver operating characteristic) curve and the optimal cut-off value derived through the diagnosis of the (CV14) PPT value for epigastric pain(心下痞硬) and the gold standard of oriental medical doctor, it was evaluated through. Results: In 353 patients, the area under the ROC curve (AUC) was 0.909 (p=0). In addition, the optimal cutting value was 10.05 (kg/cm2), which was statistically significant. Additionally, the sensitivity of the Algometer's PPT measurement was 0.704 and the specificity was 0.884. As a result, if the PPT value of the Algometer exceeds 10.05 (kg/cm²) in terms of the optimal cutting value, it can be seen that epigastric pain(心下痞硬) is lost. Conclusion: Algometer's PPT value measurement can be a reliable test method for quantification of epigastric pain(心下痞硬) diagnosis and can be useful as an objective indicator.

• The Journal of Korean Institute of Communications and Information Sciences
• /
• v.35 no.2B
• /
• pp.191-196
• /
• 2010

To increase heart disease by smoking, diabetes, obesity, stress, etc. is caused by death rate so heart disease has proposed early diagnosis necessity in modem society. Especially, incidence is on the increase rapidly because of ignorance and indifference of people about heart disease. Therefore to solve a social phenomenon about heart disease, this paper would like to design objective output parameter necessary early diagnosis of heart disease based on diagnosis theory about heart condition in the proposed Donguibogam. Specially to prove inaccurate pronunciation by heart disease would like to perform comparison, analysis of experimental group to extract vibration change rate of the vocal cords. This paper is comprised of heart disease patient and healthy people group in adult man speak to standard language then I'd like to propose early diagnosis about heart disease through comparison, analysis of vibration change rate of the vocal cords by acquisition of these voice.