• Journal of the Society of Cosmetic Scientists of Korea
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• v.34 no.1
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• pp.57-62
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• 2008

No matter how small amount of heavy metals it may be cause skin allergies through percutaneous adsorption when existing in cosmetic products as impurities. In order to develop a highly sensitive method for simultaneous determination of $Pb^{2+},\;Fe^{2+},\;Cu^{2+},\;Ni^{2+},\;Zn^{2+},\;Co^{2+},\;Cd^{2+},\;and\;Mn^{2+}$ in coloring agents and cosmetic products with rapidity and accuracy, we carried out the determination on ion chromatography. All of these metals are well separated through a bifunctional ion-exchange column(IonPac CS5A) and detected by post-column reaction and spectrophotometric detection. The calibration graphs are linear($r^2>0.999$) in the range $0.1{\sim}1000{\mu}g/mL$. Detection limits for 200 ${\mu}L$ of sample solution are at the level of ${\mu}g/L$, which is sufficient for judging whether the product is safe or not. The relative standard deviations(RSDs) of the retention time and the peak area are less than 0.21 and 1.24%, respectively. The recovery rates are $97{\sim}104%$. The new method was applied to analyze the amount of heavy metals which were contained in 22 cosmetic products and 11 coloring agents.

• Journal of the Korea Society of Computer and Information
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• v.17 no.12
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• pp.61-69
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• 2012

This paper shows results of the precise time comparison technique based on GPS code transfer in order to determine the UTC(Universal Time Coordinated) and generate TAI(International Atomic Time). CGGTTS(CCTF Group on GNSS Time Transfer Standards) which is generated by GPS timing receivers is used as the international standard format. For geodetic receivers which provide RINEX formats as GPS time transfer results, ROB(Royal Observatory of Belgium) developed a conversion program, r2cggtts, and have distributed the program to timing laboratories participating in TAI link all over the world. Timing laboratories generate the time comparison results of GPS code transfer by the program and send them to BIPM(Bureau International des Poids et Mesures) periodically. In this paper, we introduce the delay features generated while GPS code is transferred and the calibration methods of them. Then, we introduce the tropospheric delay and analyze the results of Saastamoinen model and NATO(North Atlantic Treaty organization) model. Saastamoinen model is the representative tropospheric zenith path delay model and NATO model is applied to the legacy r2cggtts program.

• Proceedings of the Korea Water Resources Association Conference
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• 2010.05a
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• pp.774-778
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• 2010

Upland erosion pollutes surface waters and often causes serious problems when deposition occurs. This study builds a sediment rating curve using the measured sediment yield and the simulated soil erosion by a GIS-embedded empirical model. The coefficient of determination ($R^2$) between the simulated soil erosion and the measurement sediment yields with rainfall amount are 0.427 for Donghyang and 0.667 for Cheonchen, but the values with rainfall intensity are 0.873 and 0.927 respectively. The data are divided into two groups: one for calibration during 2002-2005 (48 months) and the other for estimation during 2006-2008 (36 months). The first data group (2002-2005) was used to derive the SDR with an aid of soil erosion calculated by the USLE and the measured sediment yield. The mean SDR with rainfall amount is 6.273 and 3.353, respectively, while 4.799 and 2.874 for rainfall intensity. But the standard deviation (STD) with rainfall intensity is 0.930 and 0.407, which is much less than that with rainfall amount (3.746 and 2.090) for both sites. The results show the derived SDR provides reasonable accuracy and rainfall intensity gives better performance in calculating soil erosion than rainfall amount.

• YAKHAK HOEJI
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• v.51 no.2
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• pp.96-102
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• 2007

Measurement of globotriaosylceramide (Gb3, ceramide trihexoside) in urine has clinical importance for monitoring after enzyme replacement therapy in Fabry disease patients. The disease is an X-linked lipid storage disorder that results from a deficiency of the enzyme ${\alpha}$-galactosidase A (${\alpha}$-Gal A). The lack of ${\alpha}$-Gal A causes an intracellular accumulation of glycosphingolipids, mainly Gb3. A simple, rapid, and highly sensitive analytical method for Gb3 in urine was developed without labor-extensive pre-treatment by electrospray ionization MS/MS (ESI-MS/MS). Only simple 5-fold dilution of urine is necessary for the extraction and isolation of Gb3 in urine. Gb3 in diluted urine was dissolved in dioxane containing C17:0 Gb3 as an internal standard. After centrifugation it was directly injected and analyzed through guard column by in combination with multiple reaction monitoring mode of ESI-MS/MS. Eight isoforms of Gb3 were completely resolved from urine matrix. C24:0 Gb3 occupied 50% of total Gb3 as a major component in urine. Linear relationship for Gb3 isoforms was found in the range of 0.005${\sim}$5.0 ${\mu}$g/ml. The limit of detection (S/N=5) was 0.005 ${\mu}$g/ml and limit of quantification was 0.05 ${\mu}$g/ml for C24:0 Gb3 with acceptable precision and accuracy. Correlation coefficient of calibration curves for 8 Gb3 isoforms ranged from 0.9598 to 0.9975. This method could be useful for rapid and sensitive 1st line Fabry disease screening, monitoring and/or diagnostic tool for Fabry disease.

• Journal of the Korean Society of Food Science and Nutrition
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• v.45 no.11
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• pp.1604-1609
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• 2016

An HPLC analysis method was developed for standard determination of marmesin as a functional health material in Broussonetia kazinoki extract. HPLC was performed on a $C_{18}$ Kromasil column ($4.6{\times}250mm$, $5{\mu}m$) with a gradient elution of 0.1% (v/v) trifluoroacetic acid and acetonitrile at a flow rate of 1.0 mL/min at $30^{\circ}C$. The analyte was detected at 330 nm. The HPLC method was validated in accordance with International Conference on Harmonization guidelines for analytical procedures with respect to specificity, precision, accuracy, and linearity. The limit of detection and quantitation were 6.2 and $18.6{\mu}g/mL$, respectively. Calibration curves showed good linearity ($r^2$>0.9999), and the precision of analysis was satisfactory (less than 0.3%). Recoveries of quantified compound ranged from 100.35 to 101.18%. This result indicates that the established HPLC method is very useful for the determination of marker compounds in B. kazinoki extracts.

• Journal of Biomedical Engineering Research
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• v.25 no.6
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• pp.605-609
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• 2004

Blood pressure (BP) is one of the important physiological parameters for diagnosing cardiovascula diseases by means of noninvasive method. Existing noninvasive methods for measuring arterial BP have to use cuff and difficult in measuring arterial BP continuously. Systolic blood pressure (SBP) and pulse transit time (PTT) have a kind of inverse relationship. We acquired PTT data when subjects were in relaxation and also after exercise. We performed the linear regression analysis for making the regression equations for each subject and the regression equation for all subjects. We compared the estimated SBP with the measured SBP to check the accuracy of our regression equations. From the result, the regression equations for each subject was appropriate according to the American National Standards Institute of the Association of the Advancement of Medical Instrument (ANSI/AAMI) which says that BP devices should have ±5mmHg mean of error and 8mmHg standard deviation of error. However, the regression equation for all subjects was not proper to ANSI/AAMI recommendation. The result means that, without cuff, we can continuously estimate each subject's SBP through PTT and indivisual calibration.

• Analytical Science and Technology
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• v.17 no.6
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• pp.470-475
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• 2004

The mercury film thin layer flow cell (MFTLFC) which yielded the highest sensitivity for the electrochemical reduction of doxorubicin was constructed by coating the glassy carbon working electrode (GCE; $A=0.208cm^2$) with $5{\mu}L$ of HgO coating solution (0.5% HgO + 0.25% polystyrene/cyclohexanone) and subsequently followed by applying a potential of -0.40 V for 300 sec in the flow stream of an acetate buffer of pH 4.5. The voltammogram of doxorubicin reached the diffusion current plateau at -0.53 V vs. a Ag/AgCl (3 M NaCl) in the MFTLFC. The diffusion current (Id) of doxorubicin at the MFTLFC was 1.7 times greater than the Id obtained at the TLFC employing a bare glassy carbon working electrode. When the peak areas (electric charge) were plotted vs. concentrations of standard anthracyclines, the calibration factors of doxorubicin and daunorubicin were $1.12{\times}10^8{\mu}C/M$ (coefficient of determination; $R^2$: 0.969) and $0.98{\times}10^8{\mu}C/M$> ($R^2$: 0.999), respectively in the concentration range between $1.0{\times}10^{-8}M$ and $1.0{\times}10^{-6}M$.

• The Korean Journal of Pesticide Science
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• v.9 no.4
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• pp.303-310
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• 2005

This study was performed to examine analytical method of a quinolone compound, oxolinic acid in paddy soil by HPLC coupled with UV detector. Two types of soil texture in different regions were used for this experiment. Oxolinic acid was extracted by a 4 M-KOH : MeOH(1 : 3, v/v) mixtures and acidified followed by liquid-liquid partitioning in dichloromethane. Dichlormethane layer was dehydrated, evaporated and analyzed by HPLC (262 nm). Retention time was 10.2 min. The standard calibration curve of oxolinic acid showed linearity ($r^2>0.999^{**}$, y=378.99x+135.08) in the range of $1{\sim}40$ ng. The mean recoveries, evaluated from fortified soil samples at two concentration levels of 0.2 mg/kg and 1.0 mg/kg, were $90.9{\pm}4.52%$(C.V. 4.97%) and $95.0{\pm}0.23%$(C.V. 0.24%) for soil 1 and $92.2{\pm}1.15%$(C.V. 1.25%) and $93.1{\pm}0.31%$ (C.V. 0.33%) for soil 2, respectively The detection limits of two types of soils were same as 0.05 ppm. Overall, the present analytical method of oxolinic acid by HPLC coupled with UV detector seems to be used reasonably.

• Journal of the Korean Society for Nondestructive Testing
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• v.18 no.6
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• pp.455-463
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• 1998

RCCA(rod cluster control assembly) End-Tip suffers from neutron irradiation and constant vibration due to high-speed internal flow of primary coolant during plant operation. Such operating conditions cause the RCCA end-tip crackings around tile circumferential weldment of the end-tip, and in some cases, the defective end-tips were completly broken loose. However, no reliable inspection techniques for end-tip crackings were developed in the past, although some techniques exist for inspecting RCCA control rod wears. Therefore, NDE group at KEPRI has developed an ECT technique for the detection and the sizing of the end-tip crackings. The technique uses a specially designed surface-riding probe that can detect size of circumferential crackings with an accuracy of ${\pm}5.31%$ RMS error. This paper describes the ECT instrumentation including the ECT probes, calibration bars, as well as technical approaches.

• Journal of Food Hygiene and Safety
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• v.27 no.4
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• pp.332-338
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• 2012

A simple and sensitive analytical method was developed using gas chromatography with electron capture detector (GC-ECD) and gas chromatography-mass spectrometry (GC-MS) for determination of Picoxystrobin in agricultural products (apple, hulled rice, mushroom, pepper, soybean, and mandarin). Picoxystrobin residues were extracted with acetonitrile, partitioned with saline water, and then they were cleaned up on a florisil solid-phase extraction (SPE) cartridge to obtain an extract suitable for analysis by GC-ECD and GC-MS. The method was validated using 6 agricultural product samples spiked with Picoxystrobin at different concentration levels (0.02, 0.05 and 0.5 mg/L). Average recoveries of Picoxystrobin (using each concentration three replicates) ranged 64.0~98.3% with relative standard deviations less than 10%, calibration solutions concentration in the range 0.1~5 mg/L, and limit of detection (LOD) and limit of quantification (LOQ) were 0.005 and 0.02 mg/L, respectively. The result showed that the developed analytical method is suitable for Picoxystrobin determination in agricultural products.