• Parasites, Hosts and Diseases
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• 제31권3호
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• pp.193-200
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• 1993

국내의 CUPtOSPOTidiUm 인체감염 실태를 조사하기 위하여 연세대학 세브란스 병원을 찾은 230명의 외래 환자 분변을 수거하였다. Acid-fast 염색, auramine-rhodamine 염색과 CWptosporidiwpowumoocyst에 특이적인 단클론 항체를 이용한 동정법을 이용하였다. 230명의 환자 중 48명(21%)이 AF 염색법에 의하여 50명(22%)이 AR 염색법에 의하여, 그리고 23명(10%)이 단클론 항체를 이용하는 형광현미경법으로 각각 Cryptosporidium에 감염된 것으로 조사되어 국내에서도 Cryptosporidium 인체 감염이 존재하고 있는 것으로 나타났다.

• The Plant Pathology Journal
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• 제26권1호
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• pp.90-92
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• 2010

Staining profiles and bacterial morphology were compared in Xanthomonas citri pv. citri by a transmission electron microscopy. Four types of negative staining regimes were employed depending on culture media and heavy metal stains. The bacterial cells grown on LB agar media often appeared clustered on the supporting film. Meanwhile, individual bacterial cells could be readily found on the preparations from LB broth media. Typical rod-shaped cells (ca. $1\;{\mu}m$ in length) and their flagella were observed in either 2% uranyl acetate (UA) or 2% neutralized potassium phosphotungstate (PTA) staining. The UA-stained bacteria often showed relatively intact cell morphology and rather positively stained cells with a thin electron-dense stain depth around bacteria. The PTA-stained bacteria were characterized by the wrinkled cell surface where the stain was entrapped in grooves. In addition, distinct electron-dense stain depth was evident around the PTA-stained preparations. Numerous fimbriae could be mostly observed from the PTA-stained preparations of the two culture media, but not from the UA-stained preparations.

• Parasites, Hosts and Diseases
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• 제45권1호
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• pp.65-68
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• 2007

The application of Giemsa technique to stain compressed diaphragm samples obtained from rodents experimentally infected with Trichinella spiralis is described. Diaphragm samples from rats heavily infected with 20 muscle larvae per gram of body weight(20 ML/gbw) were cut into several pieces and stained with Giemsa; on the other hand, whole diaphragms from slightly infected mice(1 ML/gbw) were also stained with Giemsa. Besides, muscle samples were also stained with Giemsa. Observation at 10 $\times$ magnification revealed that both ML and nurse cells(NC) look as bluish structures clearly contrasting with the pinkish color of the non-infected muscle fibers. NC in the diaphragms of mice could be easily observed at naked eye as blue points contrasting with the pink surrounding areas formed by the non-infected muscle fibers. Among NC observed in the diaphragms of rats infected with 20 ML/gbw, 4.4% was multiple infection. These findings were confirmed in sectioned and hematoxylin-eosin stained specimens. This data could be usefulness for a rapid diagnosis of trichinellosis in post-mortem mammals without magnification procedures.

• 한국가금학회지
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• 제26권3호
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• pp.171-177
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• 1999

This study was conducted for the histological observation of the infundibulum of the oviduct of the laying Korean native pheasants. The results are as follows : 1. The infundibular wall is composed of the epithelium, lamina propria, muscle layer(inner circular and outer longitudinal muscle), and serosa. The funnel lip is divided into the inner, and outer lip of the epithelium and muscle layer. 2. The epithelium of the funnel lip and most region of the cranial part of the funnel are composed of ciliated columnar cells. In the surface and lateral part of the folds, ciliated cells and non-ciliated secretory cells tend to alternate in the epithelium of the caudal funnel and the necks, but are also found in groups of the simple cuboidal epithelium at the bases of the grooves between the ridges and tubular glands found in the subepithelium. 3. The secretory material of the non-ciliated secretory cills contains PAS-positive and alcian blue-positive granules, and these materials show purple colour in the basic fuchsin-methylene blue stain. 4. The cells of the glandular groove and tubular gland of the neck portion of the oviduct mostly show weak PAS-positive, and alcian blue stain negative reaction. The tubular gland cells of the infndibulum contain pink of purple colour granules, and without reaction in the anterior neck portion of the infundibulum in basic fuchsin and methylene blue stain.

• 대한수의학회지
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• 제35권2호
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• pp.375-381
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• 1995

Two intraocular tumors were identified in low and medium dosed groups of a carcinogenicity study using 200 males and 200 females Sprague-Dawley rats in Screening & Toxicology Research Center, Korea Research Institute of Chemical Technology. The tumors were grossly observed as white or yellow, unilateral nodules. They were approximately $1-2{\times}3-5mm$ in size. The tumors located in the region of iris and/or ciliary body invaded peripheral cornea. The microscopic features were usually composed of spindle cells arranged in parallel, forming gently curving bundles or whorls. The spindle cells had poorly defined cell boundaries, scant to moderate cytoplasm, fusiform nuclei and indistinct nucleoli. Mitotic figures were rare and areas of necrosis were present. The spindle cells had positive immunoreactivity for S-100 protein and vimentin but negative for desmin, collagen and HMB-45 antibody. In special histochemical studies, the spindle cells react with Gomori's stain for argyrophih fibers, Prussian blue stain for iron but negative with Masson-Fontana's stain for melanin granules. Ultrastructurally, cytoplasmic premelanosomes were not observed in the tumor cells due to the poor preservation of tumor masses. Based on the results, the tumors were diagnosed as amelanotic melanoma.

• 성인간호학회지
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• 제29권3호
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• pp.278-289
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• 2017

Purpose: This study was designed to investigate whether there were differences among three oral care protocols on participants' oral health status (oral status, dry mouth, halitosis, saliva pH, microorganism growth in oral cavity) and tooth stain. The three protocols were: 1) oral solution of 4% normal saline, 2) 0.1% Chlorhexidine and 3) Tantum. Methods: A nonequivalent control group pretest-posttest design was utilized. A total of 55 elderly patients residing at long-term care facilities(19 in the 4% normal saline group, 17 in the 0.1% Chlorhexidine group, 19 in the Tantum group) received oral care daily for four weeks. Data were analyzed using SPSS/WIN 22.0 program. Results: The halitosis (${\chi}^2=10.71$, p=.005) and saliva pH (${\chi}^2=6.84$, p=.033) scores were significantly improved after the oral care with 4% normal saline. Conclusion: These findings indicate that if elderly patients in long-term care facilities have complaint of the discomfort of using Chlorhexidine or Tantum, 4% normal saline is as effective at the other two. This can also be cost effective as there were no differences among the protocols in oral status, dry mouth, tooth stain or microorganism growth in oral cavity.

• 박물관보존과학
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• 제12권
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• pp.77-88
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• 2011

국립청주박물관 소장 숙명신한첩(보물 제 1629호)에 수록된 66편의 편지에 대한 보존처리 및 섬유조사를 실시하였다. 처리 전 상태는 표지가 결실되었고 본지는 습해에 의한 얼룩, 충해에 의한 결실 등의 손상이 많았으며 효종대왕 어서 1편은 별도의 액자에 보관되어 있었다. 첩을 해체하여 구 배접지 제거 후 낱장 분리된 편지에 대한 지질조사와 C-stain법을 이용한 섬유분석을 하였으며, 보존처리는 편지와 테두리의 결실부위 보수 후 액자로 분리되어 있는 편지 1편을 포함하여 전체를 하나의 첩으로 재 장황하였다.

• 한국해양학회지
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• 제24권3호
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• pp.148-156
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• 1989

본 연구는 여러 수생생태계에서 유기물의 동적순환에 따른 부유성 박테리아와 부착성 박테리아의 그램염색(gram-stain) 특성을 알아보기 위한 일련의 시도로서 일본에서 두번째로 크며 부영양화가 상당히 진전된 가스미가우라 호수에서 1년간, 매주 실시했다. 이 전형적인 부영양형(eutrophic) 수생생태계의 박테리아군집의 그램염색 특성은 중영양형(mesotrophic) 환경과 같이 유기물의 유입에 따라 변화함을 보여준다. 그램음성(negative) 박테리아군집은 부유성 박테리아군집이 약 57%, 부착성 박테리아군집이 약 53%이며, 두 군집의 통계분석은 서로 다른 특성을 보여준다. 부착성 박테리아군집은 그램 양성, 음성 모두 쇄설성 유기물 형성과 분해에 영향을 미친다. 또한 그램 음성 부유박테리아는 특히 용존 유기물의 동적순환에 관여한다.

• 대한치과보철학회지
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• 제37권4호
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• pp.416-424
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• 1999

Simulation of naturally appearing enamel in fixed prosthodontics could be achieved with all ceramics. The purpose of this study was to investigate the stain resistance of 4 different all ceramics materials (In-ceram, IPS Empress, Celay, Vintage). Forty specimens were prepared using manifacture's instruction and the samples were divided into two groups. The specimens for one group were glazing and those for other group were ground to remove the glazing layer. All specimens were immersed in methylene-blue for 24hours. The color values for each specimen was measured with colorimeter (Mode Tc-6Fx, Tokyo Denshoku Co.) prior to and after immersion in methylene-blue. The following conclusions were drawn from this study. 1 The stain resistance of glazing ceramics was higher than that of unglazing ceramics. 2. The changes of ${\Delta}E^*$\;and\;L^*$ values were revealed in Vintage and unglazing Celay. 3. The changes of $a^*$ values were revealed in unglazing vintage, Celay, In-ceram. 4. The changes of $b^*$ value were revealed in Vintage, IPS Empress and unglazing Celay.

• 한국산림과학회지
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• 제85권2호
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• pp.325-328
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• 1996

리기다소나무재의 변색 부위로부터 분리한 변색균을 다시 건전 목재에 접종하여 이 균의 목재 침입 경로를 구명한 바, 처음 균사가 목재내 방사유세포에 침입하여 방사유세포 안에서 수평 방향의 이동을 계속하다가, 시간이 경과하면 가도관내로 이동하여 이 가도관을 통로로 하여 수직 방향으로 확산을 계속하였다. 침입 90일이 지나면 목재내 각 조직으로의 침입이 완료되어 균사가 세포내강에 만연한 것을 알 수 있었다. 또한 메탄올 추출물은 변색균의 목재 침입 경로에 영향을 미치지 않는 것으로 분석되었다.