• Biomolecules & Therapeutics
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• v.24 no.4
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• pp.402-409
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• 2016

It has been found that 4-isopropyl-2,6-bis(1-phenylethyl)phenol (KTH-13), a novel compound isolated from Cordyceps bassiana, is able to suppress tumor cell proliferation by inducing apoptosis. To mass-produce this compound, we established a total synthesis method. Using those conditions, we further synthesized various analogs with structural similarity to KTH-13. In this study, we aimed to test their anti-cancer activity by measuring anti-proliferative and pro-apoptotic activities. Of 8 compounds tested, 4-methyl-2,6-bis(1-phenylethyl)phenol (KTH-13-Me) exhibited the strongest anti-proliferative activity toward MDA-MB 231 cells. KTH-13-Me also similarly suppressed the survival of various cancer cell lines, including C6 glioma, HCT-15, and LoVo cells. Treatment of KTH-13-Me induced several apoptotic signs in C6 glioma cells, such as morphological changes, induction of apoptotic bodies, and nuclear fragmentation and chromatin condensation. Concordantly, early-apoptotic cells were also identified by staining with FITC-Annexin V/PI. Moreover, KTH-13-Me highly enhanced the activation of caspase-3 and caspase-9, and decreased the protein level of Bcl-2. In addition, the phosphorylation levels of Src and STAT3 were diminished in KTH-13-Me-treated C6 cells. Therefore, these results suggest that KTH-13-Me can be developed as a novel anti-cancer drug capable of blocking proliferation, inducing apoptosis, and blocking cell survival signaling in cancer cells.

• Biomolecules & Therapeutics
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• v.24 no.4
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• pp.363-370
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• 2016

Cardiovascular disease is the most common cause of death in diabetic patients. Hyperglycemia is the primary characteristic of diabetes and is associated with many complications. The role of hyperglycemia in the dysfunction of human cardiac progenitor cells that can regenerate damaged cardiac tissue has been investigated, but the exact mechanism underlying this association is not clear. Thus, we examined whether hyperglycemia could regulate mitochondrial dynamics and lead to cardiac progenitor cell dysfunction, and whether blocking glucose uptake could rescue this dysfunction. High glucose in cardiac progenitor cells results in reduced cell viability and decreased expression of cell cycle-related molecules, including CDK2 and cyclin E. A tube formation assay revealed that hyperglycemia led to a significant decrease in the tube-forming ability of cardiac progenitor cells. Fluorescent labeling of cardiac progenitor cell mitochondria revealed that hyperglycemia alters mitochondrial dynamics and increases expression of fission-related proteins, including Fis1 and Drp1. Moreover, we showed that specific blockage of GLUT1 improved cell viability, tube formation, and regulation of mitochondrial dynamics in cardiac progenitor cells. To our knowledge, this study is the first to demonstrate that high glucose leads to cardiac progenitor cell dysfunction through an increase in mitochondrial fission, and that a GLUT1 blocker can rescue cardiac progenitor cell dysfunction and downregulation of mitochondrial fission. Combined therapy with cardiac progenitor cells and a GLUT1 blocker may provide a novel strategy for cardiac progenitor cell therapy in cardiovascular disease patients with diabetes.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.21 no.11
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• pp.180-186
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• 2020

ICT convergence using smart livestock is that in the first-generation dairy smart farm model, each device made by several manufacturers uses its own communication method, limiting the mutual operation of each device. This study uses a model based on open control technology to secure interoperability of existing ICT devices and to manage data efficiently. The open integrated control derived from this process is the software interface structure of Open API. It is an observer that serves as real-time data collection according to the communication method of ICT devices and sensors located at each end. It consists of a broker that connects and transmits to the upper integrated management server. As a result of the performance analysis through verification of two first-generation dairy smart farm model sites, the average daily milk production increased compared to the previous year (farm A 5.13%, farm B 1.33%, p<0.05). Cow days open (DO) was reduced by 17.5% on farm A and 13.3% for farm B(p<0.05). Cows require an adaptation period after the introduction of the ICT device, but if continuous effects are observed, the effect of production can be expected to increase gradually.

• Journal of Mushroom
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• v.15 no.4
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• pp.264-268
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• 2017

In the 21st century, information and communication technology (ICT) worldwide presents a new vision for agriculture. Time and place, as well as the high-tech industry, to overcome barriers to the fusion of the so-called "smart agriculture," are changing the agricultural landscape. Core container production in precision agriculture for mushroom cultivation, optimal temperature, humidity, irradiation, self-regulation of factors such as carbon dioxide, and environment for mushroom cultivation were adopted. Lentinula edodes (shiitake) is an edible mushroom native to East Asia, cultivated and consumed in many Asian countries. It is considered to be medicinal in certain practices of traditional medicine. We used different controlled light sources (Blue-Red-White-combined LED, blue LED, red LED, and fluorescent light) with different LED radiation intensities (1.5, 10.5, and $20.5{\mu}mol/m^2s$ for LEDs) to compare growth and development. Mushrooms were treated with light in a 12-hour-on/12-hour-off cycle, and maintained in a controlled room at $19{\sim}21^{\circ}C$, with 80~90% humidity, and an atmospheric $CO_2$ concentration of 1,000 ppm for 30 days. Growth and development differed with the LED source color and LED radiation intensity. Growth and development were the highest at $10.5{\mu}mol/m^2s$ of blue LED light. After harvesting the fruit bodies, we measured their weight and length, thickness of pileus and stipe, chromaticity, and hardness. The $10.5{\mu}mol/m^2s$ blue-LED-irradiated group showed the best harvest results with an average individual weight of 39.82 g and length of 64.03 mm, pileus thickness of 30.85 mm and pileus length of 43.22 mm, and stipe thickness of 16.96 mm with fine chromaticity and hardness. These results showed that blue LED light at $10.5{\mu}mol/m^2s$ s exerted the best effect on the growth and development of L. edodes (shiitake) mushroom in the ICT-system container-type environment.

• Tunnel and Underground Space
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• v.14 no.5
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• pp.327-338
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• 2004

The purpose of this research is to establish a concept of the factor of safety of tunnels which is a quantitative estimate of the stability of tunnels. Based on this concept, a numerical technique which calculates the factor of safety of tunnels was developed. To obtain the safety factor of a tunnel, the strength reduction technique in which a series of analyses are repeated with reduced ground strength until the tunnel collapses were employed. With this technique, the failure plane, as well as the factor of safety, can be obtained without prescribing the trial failure plane. Analyses were conducted with FLA $C^{2D}$(ver3.3), a geotechnical analysis program which is based on the finite difference method. From the result, the location of plastic zones, the maximum convergence and the maximum stress generated in the support system were also analyzed. The result shows that factors of safety are higher for the 1st and 2nd rock classes, and lower for the lower rock classes. Furthermore, factor of safety is higher when $K_{0}$ =0.5 compared to at in case of $K_{0}$ =2.0. Through this research, it is found that the factor of safety defined in this research can be used as a good quantitative index representing the stability of tunnels. Also, close examination of the results can help adjustment of the quantity and location of additional supports.s.

• Journal of Digital Convergence
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• v.11 no.4
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• pp.403-408
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• 2013

The configuration analysis for the image appeared in the storyboard as the 1st stage to change the scenario to the image expressed the visual phenomenon image starting from the literalism. The simultaneity of description on the interpretative level of the narrative description may describe various information multiple simultaneously, the relationship has been presented as a lot of these information has been weaved as one episode. The incident time of narrative description as the scene described mainly objectively and illustratively has been used usefully to present the accurate information for characters or incidents. The time series of narrative description appeared as one episode by expressing various spaces or images under the continuity of flow according to the time. The collision image seen from the angle of internal ignition played a role to create the meaning colliding into each other or interconnecting symbolic effects appeared as respectively fragmented image, the continuity of space played a role of expressing the different symbol when the image of completely different space is connected to one context, the continuity of time as well makes a different symbolism by interpreting symbols appeared as each images under the flow of time. These results show that the narrative description may be expressed in accordance with the narrative structure from the viewpoints of narrative description, but the internal ignition may be interpreted through the cultural and periodical background widely known in those years based on the experience and information capability the audiences are keeping not by any descriptive structure. Images appeared in the internal ignition appeared by colliding into each other or amplifying mutually no related scenes and revealing the overall symbolism by reinterpreting again.

• Journal of the Korean Institute of Intelligent Systems
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• v.13 no.2
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• pp.200-208
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• 2003

This paper proposes a separation and feature extraction of image signals using the independent component analysis(ICA) based on neural networks of efficient learning rule. The proposed learning rule is a hybrid fixed-point(FP) algorithm based on secant method and momentum. Secant method is applied to improve the performance by simplifying the 1st-order derivative computation for optimizing the objective function, which is to minimize the mutual informations of the independent components. The momentum is applied for high-speed convergence by restraining the oscillation in the process of converging to the optimal solution. The proposed algorithm has been applied to the composite images generated by random mixing matrix from the 10 images of $512\times512$-pixel. The simulation results show that the proposed algorithm has better performances of the separation speed and rate than those using the FP algorithm based on Newton and secant method. The proposed algorithm has been also applied to extract the features using a 3 set of 10,000 image patches from the 10 fingerprints of $256\times256$-pixel and the front and the rear paper money of $480\times225$-pixel, respectively, The simulation results show that the proposed algorithm has also better extraction speed than those using the another methods. Especially, the 160 basis vectors(features) of $16\times16$-pixel show the local features which have the characteristics of spatial frequency and oriented edges in the images.

• Journal of Digital Convergence
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• v.15 no.8
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• pp.277-287
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• 2017

The purpose of this study was to identify acculturative stress, social support and self esteem in migrant workers. The data of 181 subjects were collected from June 1st to September 27, 2016, and analyzed using the SPSS WIN 23.0 program. Results: There was a significant negative correlation among acculturative stress, social support(r=-.465, p<.001) and self esteem(r=-353, p<.001). Stepwise multiple regression analysis showed self esteem(${\beta}=-.21$, p<.001) and job(${\beta}=-.02$, p<.05) were significant factors affecting acculturative stress(F=27.672, p<.001). The results of the study indicate that the efforts, to improve self esteem, and to increase social support, are required to decrease acculturatives stress among migrant wokers. The results are expected to be utilized in development of supportive program for migrant workers adaptation. In the following study, the analysis of structural equation modeling about additional factors of acculturative stress among migrant workers will be needed.

• Journal of Digital Convergence
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• v.14 no.4
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• pp.285-294
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• 2016

The purpose of this research study is to analyze the influence on the necessity of establishment of eco-friendly hospital by evaluating hospital employees' awareness and their satisfaction level of working environment. Questionnaire investigation was conducted in April 2014 throughout five different medium-size hospitals in Seoul, Gyeonggi and Chungcheongbuk provinces. 213 sets of survey were used to conduct Frequency Analysis, T-test, Dispersion Analysis(ANOVA) and Structural Equation Modeling Analysis. The research concluded that employees with higher level of education, working position and longer term of working experience were more aware about Eco-friendly hospital. In addition, male, doctor, and employees with higher level of education and working position were more satisfied with their working environment. And male, employees with higher level of education and working position were more aware about necessity of establishment of eco-friendly hospital. According to the Structural Equation Modeling Analysis, the awareness of Eco-friendly hospital and the satisfaction level of working environment were the varying factors that influence the necessity of establishment of Eco-friendly hospital. Therefore, it is crucial for hospitals that are preparing for establishing Eco-friendly hospital to raise employees' level of understanding of Eco-friendly hospital and their working environment satisfaction level.

• Journal of Digital Convergence
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• v.13 no.3
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• pp.221-229
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• 2015

This study was performed to determine the occupational stress and its association with human nature factors, mental health factors and fatigue among some hospital administrative staff. The survey was administered to 189 staff in Jeonbuk area from June 1st to July 31th, 2014. The level of occupational stress was positively correlated with human nature, mental health and fatigue. The results of hierarchical multiple regression analysis show the following. The occupational stress of respondents turned out to be significantly higher in following groups: a group of female, a group that chance locus of control is higher, a group that external locus of control is lower, a group of the lower self-esteem, a group of higher situational fatigue, a group of higher psycho-social stress, and a group of higher depression. Their explanatory power was 60.4%. With the analysis of covariance structure, we could confirm relationship among the four factors such as human nature, mental health, fatigue and occupational stress. Also, the analysis showed that human nature factors were more influential on the occupational stress than mental health factors and fatigue.