• The Plant Pathology Journal
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• v.29 no.4
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• pp.465-470
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• 2013

In January 2012, spreading type petunia cv. Wave Pink plants showing an abnormal growth habit of sprouting unusual multiple plantlets from the lateral buds were collected from a greenhouse in Gwacheon, Gyeonggi Province, Korea. The presence of phytoplasma was investigated using PCR with the primer pairs P1/P6, and R16F1/R1 for nested-PCR. In the nested PCR, 1,096 bp PCR products were obtained, and through sequencing 12 Pet-Stol isolates were identified. Comparison of the nucleotide sequences of 16S rRNA gene of the 12 Pet-Stol isolates with other phytoplasmas belonging to aster yellows or Stolbur showed that Pet-Stol isolates were members of Stolbur. The presence of phytoplasma in petunia was also confirmed by microscopic observation of the pathogens. In this study, Stolbur phytoplasma was identified from spreading type petunia cultivars by sequence analysis of 16S rRNA gene of phytoplasma and microscopic observation of phytoplasma bodies. This is the first report of Stolbur phytoplasma in commercial Petunia hybrida cultivars.

• FLOWER RESEARCH JOURNAL
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• v.17 no.3
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• pp.179-184
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• 2009

The correlation and combining abilities related with plant spreading characteristics of plant height, plant width, stem length, internode length, number of stem, number of leaves, and number of flower were studied in the $F_1s$ of 10 crosses from the partial five-parent diallel cross in Petunia hybrida. The plant width showed highly positive correlation with internode length, and number of flower. The mean squares of general combining ability(GCA) and specific combining ability(SCA) were highly significant for plant width, number of stem, number of leaves, and number of flowers. The mean square values of GCA were greater than those of SCA for all the characters, showing preponderance of additive gene actions for these characters. The lines of D and I for plant width and length of stem showed relatively high GCA effects. The crosses of $D{\times}I$, $F{\times}I$, and $G{\times}I$ exhibited high SCA effects on plant width, especially $G{\times}I$ effected in internode length and number of flower. The broad sense heritability was generally high compared to narrow sense one. Plant width and stem length, and internode length related to spreading growth showed the higher heritability than the other characters in the broad and narrow sense.

• The Plant Pathology Journal
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• v.32 no.6
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• pp.545-551
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• 2016

A bacterial pathogen, Pseudomonas syringae pv. actinidiae (Psa), is a causal agent of kiwifruit bacterial canker worldwide. Psa biovar 3 (Psa3) was first detected in 2011 at an orchard in Dodeok-myeon, Goheung-gun, Jeonnam Province in Korea. In this study, we present the results of an epidemiological study regarding Psa3 occurrence on kiwifruit orchards in Korea for the period of 2013 to 2015. Since the first detection of Psa3 in 2011, there was no further case reported by 2013. However, Psa3 was rapidly spreading to 33 orchards in 2014; except for three orchards in Sacheon-si, Gyeongnam Province, most cases were reported in Jeju Island. Entering 2015, bacterial canker by Psa3 became a pandemic in Korea, spreading to 72 orchards in Jeju Island, Jeonnam, and Gyeongnam Provinces. Our epidemiological study indicated that the first Psa3 incidence in 2011 might result from an introduction of Psa3 through imported seedlings from China in 2006. Apart from this, it was estimated that most Psa3 outbreaks from 2014 to 2015 were caused by pollens imported from New Zealand and China for artificial pollination. Most kiwifruit cultivars growing in Korea were infected with Psa3; yellow-fleshed cultivars (Yellow-king, Hort16A, Enza-gold, Zecy-gold, and Haegeum), red-fleshed cultivars (Hongyang and Enza-Red), green-fleshed cultivars (Hayward and Daeheung), and even a kiwiberry (Skinny-green). However, susceptibility to canker differed among cultivars; yellow- and red-fleshed cultivars showed much more severe symptoms compared to the green-fleshed cultivars of kiwifruit and a kiwiberry.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.9 no.1
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• pp.112-120
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• 2006

It is possible to divide the historical changes of slope revegetation in Japan into five periods as follows; 1) The early period after creation(1927-1948), 2) The spreading period of modern revegetation work with manpower(1947-1958), 3) The spreading period of rapid revegetation technique using exotic grasses with machineries(1959-1985${\dots}$), 4) The spreading period of rapid reforestation technique by fast growing species mainly using leguminous shrub species(1986-1995${\dots}$), 5) The developing period of nature restoration technique using endemic arborous species(1996-). Recently main purpose of slope revegetation has been developing from the erosion protection to the nature restoration in the current of the 5th period. It is said that the role of the Japanese Society of Revegetation Technology(JSRT) is critical to the history of slope revegetation in Japan. 'The tentative guidelines of slope nature restoration' was announced by JSRT in 2004. In the guidelines, it was proposed that the planning techniques of using suitable seeds/plants based on the preservation level at the each construction sites. Moreover, the use of soil seed bank is the new and important study theme in the field of nature restoration. Consequently, at present the importance of advanced monitoring methods for vegetation maintenance and plant sociologic survey to evaluate the plant succession is increasing. Finally, some critical concepts are necessary to develop the field of restoration in Korea as follows; 1) monitoring of constructed sites, 2) ensuring of biodiversity, 3) recognition of slow revegetation and mosaic arrangement in revegetation, 4) reuse and recycle on the construction sites, and 5) promotion of specialist.

• Korean Journal Plant Pathology
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• v.14 no.6
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• pp.563-566
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• 1998

Two soybean cultivars, Kwanggyo and Hwanggeum (soybean mosaic potyvirus (SMV)-resistant cultivars), that had been inoculated with a virulent strain (G-5H, 4) of soybean mosaic potyvirus produced necrotic lesions on inoculated leaves as well as on upper trifoliate leaves. Cells in the lesion area contained sparse numbers of virus particles and very few characteristic pinwheel inclusions. Although a hypersensitive-like cellular response occurred in the two resistant cultivars, this response did not prevent the virus from spreading systemically in these resistant hosts, indicating a different mechanism from the general hypersensitive reaction in relation to host resistance.

• Journal of Bio-Environment Control
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• v.13 no.1
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• pp.21-25
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• 2004

This experiment was carried out to investigate the effect of apex removal at different leaf ages on the growth and yield of broccoli in pinching cultivation. Apex was pinched when 2, 3 or 4 true leaves were spread during nursery bed, and non-pinching was conventional method. The plant height was not different among treatments but the fresh weight was decreased as the pinching days were delayed. The numbers of leaves and lateral shoots were increased compared to those of non-pinching by pinching. Days to central budding were shortended by 3 days when pinched at 2 true leaves spreading stage than those of non-pinching. Central (1st) head weight was heaviest when pinched at the time of 2 true leaves spreading (157g). Total head weight was heaviest when pinched at the time of 2 true leaves spreading (378g), and lowest at 4 true leaves spreading stage (196g). Marketable yields were increased by 48% when pinched at the time of 2 true leaves spreading (1,754kg) as compared with those of non-pinching (1,184kg) and lowest when pinched at the time of 4 true leaves spreading (820kg). Also, first harvest was shortended by 6 days by pinching at 2 true leaves spreading stage.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2020.08a
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• pp.15-15
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• 2020

Commelina caroliniana Water, was founded in Jongdal-ri (Gujwa-eup, Jeju Island) for the first time, is widely distributed arable land near the coast and roads. C. caroliniana belongs to Commelinaceae and native to Bangladesh, India. This species can be distinguished from its allied species in Korea by several characters as followings: apex acuminate and obovate shape spathes, flowers have blue 3 petals, maroon spot on the antherode, fruit with 5 seeds in 3 ovaries. C. caroliniana is well known for its strong fertility, which can outcompate native grasses and further negatively effect on the grassland ecosystem. The species has already introduced and widely distributed across much of southeastern US and some of Asian countries. Considering the case of C. caroliniana's diffusion capacity and influence on the ecosystem, management system for prevent their spreading are required through monitoring. Given the great potential of spread found in C. caroliniana, continuous monitoring may be required.

• Magazine of the Korean Society of Agricultural Engineers
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• v.29 no.4
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• pp.93-105
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• 1987

This Study was carried out at the experimental Plot of Kang-Weon Province, Institute of Agriculture experiment, to find out Irrigation Water requirement and suitable calculating formula of evapotranspiration on Spreading Varieties of rice plant such as Seul Oak, Bokkwang and Teaback in Chuncheon Area. The evapotranspiration, infiltration, and consumptive use of water were measured by Micro Lysimeter for four years from '86yr. Also, yield of rice was investigated during same period. With the Kc Value taken from experimental value, evapotranspiration was calculated by methods of Blaney & criddle, Penman, Hargreaves and Thornthwaite by Computer using meteorological data in Chuncheon Area for twenty one yrs from '66yr to '86yr. All analyses were conducted based on average value of experiment for four years and the results are summarized as follows : 1) The yield by varieties through this experiment showed 1.06 times in Seul Oak, 1.94 times in Bokkwang and 1.89 times in Teaback more than Standard Yield. 2) The consumptive use of water including infiltration were 1.068.4mm in Seul Oak, 1,102. 6mm in Bokkwang and 1,195.6mm in Teaback 3) The evapotranspiration by Actual measurement presented 520.lmm in Seul Oak, 540.lmm in Bokkwang and 598.4mm in Teaback 4)The ratio of evapotranspiration and infiltration over Panevaporation showed 1.2 to 1.4. 5) The irrigation water reguirement by water balance were shown to be irrigated more than 584 mm / yr in average during 21 years from '66yr to '86yr for all Varieties and those for loyr frequency 693 mm in Seul Oak, 712 mm in Bokkwang and 728 mm in Teaback respectively. 6) Crop Coefficient (Ke Value) of the tested rice plant during the period were shown as Table 10. 7) Penman Method was the formula the most close to experiment Value among four different methods of Blaney & Griddle Penman, Hargreaves, and Thornthwaite.

• Mycobiology
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• v.46 no.4
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• pp.416-420
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• 2018

Crepidiastrum sonchifolium, a flowering plant in the daisy family (Asteraceae), is native to East Asia. In Korea, this plant is a locally cultivated vegetable, and its market size is gradually growing. Since the plants with downy mildew infection were initially found at a private farm of Chuncheon city, the occurrences have continued in commercial farms of other regions, highlighting that this disease is spreading throughout Korea. The pathogen was attributed to a member of the genus Bremia that contains many specialized species, each of which displays a narrow host spectrum on Asteraceae. Based on morphological and molecular phylogenetic analyses, along with the high host specificity recently proven for Bremia species, the identity of the causal agent was confirmed as a so far undescribed species of Bremia. Here, we introduce Bremia itoana sp. nov., specific to C. sonchifolium.

• Proceedings of the Korean Society of Plant Pathology Conference
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• 2003.10a
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• pp.72.1-72
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• 2003

Generally, plants defend themselves against pathogens by structural and biochemical reactions. Defense structures act as physical barriers and inhibit the pathogen from gaining entrance and spreading through the plant. Xanthomonas axonopodis pv glycines, the causal pathogen of bacterial pustule of soybean, causes hypersensitive response (HR). When pepper fruits were inoculated with X. axonopodis pv. glycines, in situ, time-series defense-related structural changes occurred in the inoculated sites. Early responses were programmed cell death (PCD), characterized by condensation and vacuolization of the cytoplasm, condensation of nuclear materials, and fragmentation of the nuclear DNA, which were observed by transmission electron microscopy. Nuclear fragmentation was proven by TUNEL method under confocal laser scanning microscopy and DNA laddering through eletrophoresis. At later stages, plant responses were cell elongation and cell division, forming a periderm-like boundary layer that demarcated healthy tissues from the inoculation sites. Using several stains such as toluidine blue, sudan IV, annexin V, and phloroglucinol-HCl, defense-related materials and structural changes were also examined.