• Journal of Mushroom
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• v.8 no.4
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• pp.161-164
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• 2010

For the development of a sporeless strain of P. ostreatus we used sporeless strain ASI 2069. We have recovered both nuclear types of strain ASI 2069 as monokaryons of nh9, nh15, nh26 and nh36 (here after referred to as neohaplonts) by protoplasting the mycelium. Crosses between neohaplonts and SSI's(single spore isolates) obtained from a sporulating commercial strain ASI 2180. Five excellent strains are selected from 30 bred strains by quality of fruitbodies and spore number. To development of molecular markers linked to sporeless strain of P. ostreatus, we are screened helicase, recombinase(DMC1) and topoisomerase(Spo11) genes related meiosis by PCR and sequencing. Among three genes, Spo11 gene was identified into molecular marker of sporeless from neohaplonts and bred strains of P. ostreatus.

• Journal of Mushroom
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• v.4 no.2
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• pp.53-56
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• 2006

The enormous production of spores by the fruitbodies in the cultivation of oyster mushrooms (Pleurotus ostreatus) is develop an allergy with symptoms similar to an "extrinsic allergic alveolitis (EEA)". the sporeless strain would noy only benefit health of mushroom workers but also reduce the risk of viral infections on the mushroom farms. For the development of a sporeless strain of P. ostreatus we used strain ASI 2069. This non-commercial strain is completely nonsporulating. We have recovered both nuclear types of strain ASI 2069 as monokaryons (hereafter referred to as neohaplonts) by protoplasting the mycelium. Crosses between neohaplonts and SSI's(single spore isolates) obtained from a sporulating commercial strain ASI 2180 yielded fruitbodies that isolated 128 strains. 13 excellent strains are selected from 30 bred strains by quality of fruitbodies and spore number. Among 13 excellent strains, G192 strain is chose finally to high yield and sporelessness.

• Proceedings of the Korean Society for Applied Microbiology Conference
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• 1986.12a
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• pp.521.1-521
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• 1986

B. sphaericus의 sporeless ts-D1216 돌연변이체의 유전학적 특성을 방사성 동위원소를 이용하여 DNA의 합성과 RNA의 합성을 측정하였고 일반적 특성을 연구하였다. ts-D1216 돌연변이체의 대수증식기 세포를 3$0^{\circ}C$에서 제한온도 (42$^{\circ}C$)로 이동시켰을 때 RNA의 합성은 4-5시간 정상적으로 합성이 계속 일어났고, DNA의 합성은 60-100분까지는 정상적인 율로 일어나다가 그 후에 결정적으로 감소되었다. 또한, DNA합성이 멈춘후에도 세포수는 증가했고, 4$0^{\circ}C$에서 성장기간이 더 길면 길수록 DNA합성에의 회복능력이 상실되었다.

• The Korean Journal of Mycology
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• v.12 no.4
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• pp.164-169
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• 1984

Interspecific heterokaryons were obtained between auxotrophic mutants of Pleurotus ostreatus and Pleurotus florida by polyethylene $glycol-Ca^{++}$ induced fusion of somatic protoplasts. The fusion products produced colonies of dense growing mycelium after 10-14 days culture on hypertonic Mushroom Minimal Medium. When they were transferred to Mushroom Minimal Medium plates, the sectors showed normal vegetative morphology but the colonies of irregular shape varied in growth rate. All of the colonies produced fruit body of normal pilei. Some heterokaryon colonies bearing none or only a small amounts of basidiospores were isolated. Fusion products, generally, gave higher basidiocarp yields than the parents.