• Pediatric Infection and Vaccine
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• 제6권2호
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• pp.253-260
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• 1999

목 적 : human CMV가 생성하는 분자량 72,000 dalton의 immediate early protein(p72)과 반응하는 단세포군 항체를 국내에서 분리된 CMV 야생주로 직접 제작하여 진단에 사용해 보기 위한 것이다. 방 법 : 정상 인체 섬유아세포(Foreskin 유래)를 10% 우태아혈청이 함유된 Eagle' s minimal essential medium(MEM)에서 배양하여 사용하였으며 마우스 골수종세포는 P3X63 Ag8.653(ATCC, Mary land USA)을 $5{\times}10^5/ml$ 세포수를 유지하도록 배양하였다. 사용된 항원은 선천성 CMV 감염아의 소변으로부터 검출한 CMV(KJHJ90)를 대량 증식시켜 사용하였다. 생후 6~8주된 Balb/c마우스의 복강내에 CMV 항원을 1주일 간격으로 4회 주사한 후 면역시킨 마우스 비장에서 얻은 임파구와 형질세포종세포로 최적발육기의 P3X63-Ag8.653을 사용하여 융합하였다. 융합세포를 배양한 후 간접면역형광항체법을 이용하여 항체가가 높은 것을 골랐다. 결 과 : 생산된 28종의 단세포군항체 중 그림 1과 같이 LPC12와 LPC23클론이 AD169에 감염된 세포의 핵에 특히 강하게 반응하였다. 정제된 AD169를 SDS-PAGE한 후 Western blotting을 하여 생산된 단세포군항체가 반응하는 항원의 분자량을 확인하였으며 그 중 LPC12와 LPC23클론에서 생성된 단세포군항체는 약 72KDA의 항원과 특이적으로 가장 강하게 반응하였다. 결 론 : 본 연구에서 제작된 LPC12와 LPC23 클론에서 생성된 단세포군 항체를 이용하여 선천성 CMV감염으로 확인된 소아의 소변을 이용하여 CMV AD 169 주 유래 항체(P63-27)과 비교한 DEAFF 검사상 동일한 결과를 확인한 바 LPC12와 LPC 23 클론 항체는 AD 169(P63-27)과 같이 CMV 감염의 조기진단에 사용할 수 있을 것으로 사료된다.

• 수산해양교육연구
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• 제27권6호
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• pp.1656-1664
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• 2015

고삼 뿌리 열수 추출물을 농도별로 넙치 사료에 흡습시켜 12주 동안 어류에 급여 후 비특이적 면역 및 질병저항성에 대한 효과를 조사하였다. 고삼 추출물의 첨가는 어류 성장에는 아무런 영향을 미치지 않았다. 혈액화학적인 조사 결과에서도 고삼 첨가로 인한 넙치의 생리에는 아무런 영향을 나타내지 않았다. 라이소자임 활성은 0.05%의 고삼 추출물 농도로 흡습시킨 사료 그룹에서 라이소자임 활성이 증가됨을 알 수 있었다. 백혈구의 식세포 활성은 0.05%의 고삼 추출물로 흡습시킨 사료 그룹에서 유의적으로 증가되었다. 병리학적 결과를 통하여 고삼 추출물을 농도별로 넙치에 투여하였을때 병리조직학적 영향이 없음을 알 수 있었다. 에드와드균의 인위감염에 의한 질병저항성은 0.05%의 고삼 추출물로 흡습시킨 사료 그룹에서만 상대생존율이 높게 나타났다. 이상의 결과를 종합하여 볼 때 고삼뿌리 열수 추출물은 넙치에 대한 비특이적 면역력 증강 및 질병 저항성 증강에 효과가 있는 것으로 사료되었다.

• 한국식품과학회지
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• 제38권6호
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• pp.829-834
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• 2006

HIV 감염 후 AIDS로의 진행과정에서 cytokines의 변화는 필수적인 결과로서 나타나게 된다. 본 연구에서는 AIDS 동물모델을 사용하여 Pyc Cytokines발현에 미치는 영향과 조절 매개체로서 $NF-{\kappa}B$의 관련성 및 작용 기작 확인에 초점을 두었다. LP-BM5 retrovirus에 감염된 C57BL/6 생쥐를 이용하여12주간에 걸친 실험에서Pyc 투여는 Th1과 Th2 cytokines의 혈중농도를 조절하여 murine AIDS로의 진행을 억제하는 데 역할을 하는 것으로 사료된다. 특히 Th2 cytokines의 mRNA 발현을 억제함에 따라 Th1 cytokines의 혈중농도는 상대적으로 증가한 것으로 사료된다. 이러한 결과는 Pyc가 Th2 cytokines을 선택적으로 transcription level에서 조절하고 있음을 의미하고 있다. 또한 Th2 cytokines mRNA 발현 조절은 $NF-{\kappa}B$의 활성화에 의한 것으로 추정된다. 본 연구는 AIDS 동물모델에서 $NF-{\kappa}B$의 조절을 통한 cytokines의 발현 조절의 가능성을 제시함과 동시에 Pyc의 역할에 대해 자료를 제시하고 있다. 또한 향후 murine AIDS 진행 억제제로서의 Pyc의 기작에 대한 일면을 보인 것에 의의를 두고자 한다.

• 동의생리병리학회지
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• 제21권5호
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• pp.1210-1218
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• 2007

Recently Atopic Dermatitis(AD) is increasing along with allergic disease. At present, there is no infallible cure for AD. Then AD patients undergo great suffering. This study is carried out to see whether or not the administering Danggwieumja(DG) along with Samhwangseje-gamibang(SG} as a medicine for external aplication, is effective in treating atopic dermatitis. To examine the effectiveness of the above prescription, the author made an observation of diverse immune responses. through the model of NC/Nga atopic mice. Results provided evidence that the DG administration along with SG can be used as a treatment means to atopic dermatitis. The results are as follows: The extent of Clinical skin severities in 13 and 16 week old NC/Nga mice treated with DG and SG, were reduced by 50.9%, 53.9% respectively, compared to the control NC/Nga mice with no drug treatment. IgE, IL-4, IL-5, IL-6, IgM and IgG1 levels in the serum of the NC/Nga mice treated with DG and SG were significantly decreased compared to those of the untreated control mice. In contrary, to the $IFN-{\gamma}$ level, significantly increased. The spleen weight of the NC/Nga mice treated with DG and SG significantly decreased compared to those of the untreated control mice. CCR3 gene expression in the skin tissue of NC/Nga mice treated with DG and SG were highly decreased, and the IL-6 expression significantly decreased, and the $IFN-{\gamma}$ gene expression increased compared to those of the untreated control mice. Histological observation of the ear and dorsal skin tissue of the NC/Nga mice treated with DG and SG, showed that the extents of inflammation and infiltrated immune cells in the epidermal tissue and dermis, were highly reduced compared to those of the untreated control mice. In the model inducing COX-2 activity in RAW 264.7 cell, the denser DG became, the more COX-2 activity was inhibited, compared to those of the untreated control group. $IL-1{\beta}$, and $TNF-{\alpha}$, IL-6 gene expression in RAW 264.7 cell with DG, significantly decreased, compared to those of the untreated control group. According to the assessment of cell toxicity in L929 cell, the rate of cell multiplication increased by 3% in consistency to 100ppm of DG compared to the untreated control group and in more than the 200 ppm consistency, cell toxicity was occurred.

• 한국어병학회지
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• 제4권2호
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• pp.59-70
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• 1991

Ceroid증(症)의 치료효과(治療效果)를 알기 위한 혈액(血液) 성상(性狀)은 다음과 같다. 1. 넙치 혈구상(血球像)의 모든 값은 glutathione을 투여(投與)한 7일(日)째에 급격(急激)히 증가(增加)하기 시작(始作)하여 28일(日) 이후(以後)부터는 거의 정상치(正常値)를 유지하였다. 2. 총단백(總蛋白), 혈당(血糖), 인(燐), 무기인(無機燐), 혈액(血液) 요소성(尿素性) 질소(窒素), 총(總) 콜레스트롤, 중성지방(中性脂肪), GOT 및 GPT 값은 투여(投與) 7일(日)째에 급격(急激)히 감소(減少)하기 시작(始作)하여 21일(日)째부터는 정상치(正常値)를 유지 하였다. 3. 1일(日) 1kg 먹이량에 5mg 10mg 첨가(添加)하여 3주일간(週日間) 투여(投與)하면 넙치의 ceroid증(症)이 치료(治療)되는 것을 알 수 있었다.

• 한국식품과학회지
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• 제32권4호
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• pp.914-919
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• 2000

갈근은 두과식물로서 우리나라를 비롯한 동남아 지역에서 자생하는 식물로 간질환 치료에 많이 이용되어 오는 전통 식품으로 음용수 대신 3% 갈근 추출물과 납을 흰쥐에게 4주 동안 경구 투여 한 후 각 장기 무게, 간, 폐, 위, 신장, 심장 및 비장 조직의 납함량과 혈청중의 GPT, GOT, LDH, ChEase의 함량을 조사하였다. 최종일 체중을 보면 대조군이 298.15 g으로 납-단독 투여군은 $203.38{\sim}214.76\;g$으로 대조군에 비하여 감소하였다. 각 장기 무게에서 대조군은 갈근 추출물에 비하여 전체적으로 증가하였고, 납 단독 투여군은 갈근추출물-납 동시 투여군에 비하여 감소 하였다. 각 조직의 납 함량에서 납 단독 투여군은 대조군, 갈근추출물-납 동시 투여군에 비하여 증가하였으며 각 군간에 유의적인 차이가 인정되었다. GPT, GOT의 활성도에서 대조군은 각각 78.07, 104.80, 갈근 추출물군은 각각 63.46, 96.44로 대조군 보다 감소하였고, 납 단독 투여군은 $83.96{\sim}88.02,\;133.07{\sim}139.62$, 갈근추출물과 납 동시 투여 군에서는 $43.50[\sim}51.40,\;96.00{\sim}99.80$으로 납 단독 투여군에 비하여 유의적으로 감소하였다. LDH에서 대조군은 499.33, 갈근 추출물군은 213.60으로 대조군에 비하여 감소하였으며, 납 단독투여 군은 $595.30{\sim}615.50$, 갈근추출물과 납 동시 투여군은 $161.80{\sim}247.00$으로 납 단독 투여군 보다 LDH활성이 감소되었다. ChEase 활성은 대조군은 70.60, 갈근 추출물은 81.60으로 대조군보다 약 15.6% 증가하였고, 납 단독 투여군은 갈근 추출물-납 동시 투여군에 비하여 약 $42{\sim}73%$의 증가를 가져왔다.

• 한국응용과학기술학회지
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• 제35권3호
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• pp.787-796
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• 2018

본 연구는 열 스트레스 (heat stress, HS)에 노출된 산란계에서 혼합 생균제의 급여가 생산성, 계란품질, 면역반응, 맹장 미생물 및 분 암모니아에 미치는 효과를 조사하였다. 총 400마리의 50주령 Hy-Line brown 산란계를 무작위로 각각 100마리씩 4 그룹, C (대조군, 실온 $25^{\circ}C$), HS (열 스트레스 $33^{\circ}C$), HSP (HS 플러스 혼합 생균제 500, 750 mg/kg)로 배치하였다. 산란계의 생산성, 계란품질, 비장 무게, 혈액 IgG 및 lymphocyte 농도는 HSP 그룹에서 HS 그룹과 비교했을 때 증가하였고, 코르티코스테론, heterophil과 lymphocyte의 비율 (H:L) 및 폐사율은 유의하게 낮았다. Lactobacillus는 HSP 그룹에서 HS 그룹과 비교했을 때 증가하였으나 Escherichiacoli (E. coli), coliform bacteria 및 aerobic bacteria는 유의하게 낮았다. 분에서 암모니아 발생은 HS 그룹이 HSP 그룹에 비해서 유의하게 증가하였다. 결론적으로, 이러한 혼합 생균제가 여름철 산란계의 더위 피해를 방해주고 면역반응, 맹장 미생물 균형을 경유하여 생산성, 계란품질 및 계분으로부터 악취 발생을 줄이는 데 효율적인 영양전략이 될 수 있음을 나타낸다.

• 한국응용과학기술학회지
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• 제30권3호
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• pp.400-410
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• 2013

This study was carried out to investigate the effects of feeding the broilers that are exposed to extreme heat stress by control of inverse lighting times with night restricted feeding of extreme heat diet(EHD1, 2: extreme heat diet) containing different amount of soy oil, molasses, amino acids and vitamin C on short chain fatty acid and blood lipid profile. 300 broiler chickens(Abaica strain) were randomized into four dietary treatment groups according to a randomized block design on the day they were hatched. The four dietary treatment groups were: T1(EHD 1, 10:00~19:00 Dark, 19:00~10:00 Light), T2(EHD 2, 10:00~19:00 Dark, 19:00~10:00 Light), T3(EHD 1, 09:00~18:00 Dark, 18:00~09:00 Light), T4(EHD 2, 09:00~18:00 Dark, 18:00~09:00 Light). The body weight gain of the broilers was highest in T2, and high in order T1, T4, T3(p<0.05). Weights of the lymphoid organ, thymus and bursa of Fabricius were high in T1, T2 as compared to T3, T4 but spleen was lower in T4 than T1, T2, T3(p<0.05). Blood triglyceride, total cholesterol and glucose were higher in T1, T2 than T3, T4(p<0.05). LDL-C was high in orderT4, T3, T2, T1 but HDL-C showed the opposite trend(p<0.05). Blood concentrations of IgG, IgG and IgM were higher in T1, T2 than inT3, T4, but the corticosterone concentration decreased significantly in them. In T1 and T2, Lactobacillus in the feces increased, but total aerobic bacteria, E.coli, coliform bacteria was decreased rather significantly, compared with those in T3 and T4(p<0.05). Concentrations of acetic acid, propionic acid and total SCFA in cecum were high in order T2, T1, T3, T4, but butyric acid, isobutyric acid, valeric acid, isovaleric acid were lower in T1, T2 than in T3, T4 (p<0.05).

• Journal of Nutrition and Health
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• 제6권4호
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• pp.15-22
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• 1973

Sodium chloride plays an important role as the main condiment at daily meal. It is well known that humans require sodium chloride as an essential nutrient to keep the homeostasis of electrolytes. The amounts of salt intake may be a reflection of geography, culture and food habit rather than necessity. Lee has reported (1962) that Koreans ingest high amounts of sodium chloride in their meals, with an intake of excess carbohydrate (80-90% of total Calories) and low protein in their diet. This includes large amounts of rice, Kimchi and other fermented soybean products common in the Korean diet. This investigation was designed to study the dietary relations of sodium chloride to other nutrients in the Korean diet. Twenty four albino male rats, weighing from 290-300g, were divided into four dietary groups according to the amounts of carbohydrate, protein and fat in the basal diet. Each diet contained a rice powder as a carbohydrate source. Diet I was a control diet, Diet II, low protein, Diet III, low protein and low fat diet and Diet IV, low fat diet. All rats were provided with 3% sodium chloride solution. Diet and salt solution were given ad libitum. The experiment was carried out for 9 weeks during which time the body weight, the food intake, and 3% sodium chloride solution consumption were determined. At the 9th week, the urine was collected the blood sample from the artery of each rat for the analysis of sodium and potassium and other chemical studies. The rats were sacrificed and the kidney, adrenal, liver and spleen were measured, and observed changes of the pathological tissue in the kidney and adrenal. The results were summarized as follows: 1) The growth rate was higher in Diet I than in the other experimental diets (II, III and IV) after 4 weeks. There was no significant difference found between the experimental Diets II, III and IV. 2) The daily food intake was greater in the experimental diets II, III and IV than in the control diet. However, there was no difference among the high carbohydrate diets Diet II, III and IV. 3) The daily water (3% sodium chloride solution) intake was also greater in the Diets II, III and IV, than in the control diet. However, there was no difference between Diets II, III and IV. 4) The concentration of sodium and potassium in the blood were within the normal range in all diets. 5) The amount of sodium chloride in the urine was significantly greater in Diets II, III and IV than in the control diet. Diets II, III, IV had a larger amount of sodium solution consumption. 6) Observation of pathological tissue in the experimental diets found a cell proliferation in the glomerlulus of the kidney, while such change was not found in the control diet.

• 한국응용과학기술학회지
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• 제5권2호
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• pp.29-38
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• 1988

This study was done to determine the effect of antioxidants added perilla oil diet on the content of cholesterol, vitamin E, and lipid peroxide in serum and tissue of rats. Four groups of experimental diets, such none added perilla oil diet, ascorbic acid added perilla oil diet, vitamin E added perilla oil diet, EDTA added perilla oil diet were fed ad libitum to the 4 weeks white male rats of Sprague-Dawley strain. The results obtained are summarized as follow: 1) The body weight gain in all experimental diet groups was higher than the control gorup and EDTA added diet group was lower than the other experimental diet group, while food intake in vitamin E added diet group was the highest and vitamin C added diet group was the lowest in the control group. 2) Total cholesterol levels in serum of all experimental diet groups were lower than that of the control group and especially the level of total cholesterol in none added diet group and vitamin C added diet group were significantly lower than that of control group. 3) HDL-cholesterol levels of all experimental diet groups were lower than that of the control group and especially none added diet group was significantly lower than that of control group. 4) The activities of glutamic oxaloacetic transaminase (GOT) in serum of all experimental diet group except EDTA added diet group were higher than that of the control group and especially none added diet group was significanly higher than that of the control group. The activites of glutamic pyruvic transaminase (GPT) in serum of all experimental diet groups except vitamin C added group were higher than that of control group. 5) Vitamin E levels in serum of none added diet group and vitamin C added diet group were lower than that of the control group and vitamin E added diet group and EDTA added diet group were higher than that of the control group. 6) Vitamin E levels in liver of all experimental diet groups were higher than that of control group and especially none added diet group and vitamin E added diet group were significantly higher than that of the control group. 7) Lipid peroxide in serum of all experimental diet group were lower than that of control group and especially EDTA added diet group. 8) Lipid peroxide in liver and spleen of all experimental diet groups were higher than that of the control group and lipid peroxide in kidney of all experimental diet groups except EDTA added diet group were higher than that of the control group. Four these results, as vitamin C, vitamin E and EDTA added diets have an effect to lipid peroxide by antioxidants, it could be suggested that perilla oil diet has required to add antioxidant because it has not sufficient vitamin E for antioxidant and intake and overtake level of perilla oil diet should be studied to go ahead.