Journal of Fisheries and Marine Sciences Education (수산해양교육연구)

The Korean Society for Fisheries and Marine Sciences Education (한국수산해양교육학회)
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Effect of Disease Resistance on Oral Administration of Lightyellow Sophora Extract in Olive Flounder

생약제 고삼 뿌리 열수추출물의 넙치 투여시 질병 저항성에 미치는 영향

  • SEO, Jung-Soo (National Fisheries Research and Development Institute) ;
  • JEON, Eun-Ji (National Fisheries Research and Development Institute) ;
  • KWON, Mun-Gyeong (National Fisheries Research and Development Institute) ;
  • HWANG, Jee-Youn (National Fisheries Research and Development Institute) ;
  • KIM, Jin-Do (National Fisheries Research and Development Institute) ;
  • JUNG, Sung-Hee (National Fisheries Research and Development Institute) ;
  • KIM, Na-Young (National Fisheries Research and Development Institute) ;
  • JEE, Bo-Young (National Fisheries Research and Development Institute) ;
  • PARK, Myoung-Ae (National Fisheries Research and Development Institute)
  • Received : 2015.09.23
  • Accepted : 2015.10.16
  • Published : 2015.12.31
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Abstract

The effects of lightyellow sophora, Sophora flavescens extract were tested on non-specific immune response and a disease resistance of olive flounder, Paralichthys olivaceus. For feeding trial, weight gain of fish fed different treated groups were not significant differences. Serum analysis of treated and control group did not demonstrated any differences. Lysozyme activity in the kidney and spleen of the 0.05% treated group on $4^{th}$ and $8^{th}$ week showed significant increase, respectively. In addition, phagocytic activity of the 0.05% treated group on $4^{th}$ and 8th week showed significant increase compared to the control group. Histopathology of the liver and kidney tissue of the administrated group for $4^{th}$, $8^{th}$ and $12^{th}$ week showed no particular signs of tissue degeneration. The treated group was higher than control group by analyzing the relative percent survival (RPS) of the experimental challenge of Edwardsiella tarda. Therefore, the lightyellow sophora showed effective in preventing fish-pathogenic edwardsiellosis for a certain period of time.

고삼 뿌리 열수 추출물을 농도별로 넙치 사료에 흡습시켜 12주 동안 어류에 급여 후 비특이적 면역 및 질병저항성에 대한 효과를 조사하였다. 고삼 추출물의 첨가는 어류 성장에는 아무런 영향을 미치지 않았다. 혈액화학적인 조사 결과에서도 고삼 첨가로 인한 넙치의 생리에는 아무런 영향을 나타내지 않았다. 라이소자임 활성은 0.05%의 고삼 추출물 농도로 흡습시킨 사료 그룹에서 라이소자임 활성이 증가됨을 알 수 있었다. 백혈구의 식세포 활성은 0.05%의 고삼 추출물로 흡습시킨 사료 그룹에서 유의적으로 증가되었다. 병리학적 결과를 통하여 고삼 추출물을 농도별로 넙치에 투여하였을때 병리조직학적 영향이 없음을 알 수 있었다. 에드와드균의 인위감염에 의한 질병저항성은 0.05%의 고삼 추출물로 흡습시킨 사료 그룹에서만 상대생존율이 높게 나타났다. 이상의 결과를 종합하여 볼 때 고삼뿌리 열수 추출물은 넙치에 대한 비특이적 면역력 증강 및 질병 저항성 증강에 효과가 있는 것으로 사료되었다.

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