• Korean Journal of Fisheries and Aquatic Sciences
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• v.50 no.4
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• pp.429-436
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• 2017

The seasonal and interannual variations in fish assemblages in the coastal waters off Gijang were examined with an otter trawl from 2015 to 2016. A total of 59 fish species was collected during the study period. The dominant species were Caelorinchus multispinulosus, Clupea pallasii pallasii and Chelidonichthys spinosus. Seasonal changes in fish assemblages appeared to be distinct in the study area. The fish assemblages in summer and autumn were clearly distinguishable from those in other seasons, and the largest numbers of species and individuals were captured in winter and summer, respectively. Because fish assemblages are closely related to changes in water temperature, long-term changes in water temperature can have a significant impact on the productivity of offshore fisheries by changing fish species composition, habitat migration, and growth rate of fish.

• Archives of Pharmacal Research
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• v.16 no.4
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• pp.289-294
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• 1993

A binding protein of radio-labeled sanjoinine-A (fangufoline) in rat brain cytoplasm was investigated, using an equilibrium dialysis technique. The labeled agent was bound to the cytosol fraction with two distinctly different types of sets in calclum ion-dependent manner. The bound protein was identified as calmodulin by dgel filtration of the sanjoinine-A bound cytosol fraction on a Sephadex G-75 column. Calmodulin was bound to sanjoinine-A bound at two sets of binding sites in the calculated as two at high affinity sites $(Kd=1.1\;mu{M)}$ and four at low affinity sites $(Kd=3.1\;\mu{M)}$.

• Archives of Pharmacal Research
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• v.24 no.3
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• pp.202-206
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• 2001

The effects of various sedative cyclopeptides and peptide alkaloids from the Zizyphus species on calmodulin-dependent $Ca^{2+}$ -ATPase and phosphodiesterase were Investigated. Calmodulin-induced activation of $Ca^{2+}$-ATPase was strongly inhibited by sanjoinine-A dialdehyde (IC_{50}$, 2.3$\mu\textrm{m}$), -Ah1 (IC_{50}$, 4.0$\mu\textrm{m}$), -A (IC, 4.6$\mu\textrm{m}$), and -G2 (IC_{50}$, 7.2$\mu\textrm{m}$), while calmodulin-induced activation of phosphodiesterase was strongly inhibited by both deachuine- S10 (IC_{50}$, 4.9$\mu\textrm{m}$) and sanjoinine-D (IC_{50}$, 9.0$\mu\textrm{m}$). The inhibitory activity of the various cyclopeptides and peptide alkaloids on $Ca^{2+}$-ATPase was found to correlate well with their Sedative activity.

• Toxicological Research
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• v.12 no.2
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• pp.181-194
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• 1996

The effects of Sanjoinine-A, an alkaloid isolated from Zizyphus spinosus semens, on central nervous system and general pharmacology were studied. In summary, Sanjoinine-A depress the spontaneous locomotor activity without motor incoordination and it has slight analgesic effect. Those effects are qualitatively similar to that of diazepam but its potency is much lower than diazepam(20 times). Sanjoinine-A does not depress the electric or pentylenetetrazole induced convulsion. Those effects are dissimilar with that of diazepam. Sanjoinine-A slightly depress the spontaneous or acethylchollne induced motility of smooth muscles but degree of depressant effect was variable to tissues. Sanjoinine-A does not show any effects on digestive system, blood, kidney fuction and neural ganglion.

• Korean Journal of Pharmacognosy
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• v.16 no.4
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• pp.233-238
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• 1985

A number of sedative alkaloids were isolated from Sanjoin(酸棗仁), the seeds of Zizyphus vulgaris Lamark var. spinosus Bunge (Rhamnaceae) which is an important Chinese medicinal material used to treat insomnia and sometimes to treat sleepiness. Those compounds were designated as Sanjoinine-A, B, C, D, etc. depending on the order of increasing polarity. Sanjoinine-A, $C_{31}H_{42}N_4O_4$, $mp\;249^{\circ}$, $[{\alpha}]^{27}_D-316$, Sanjoinine-B, $C_{30}H_{40}N_4O_4$, $mp\;212{\sim}4^{\circ}$, Sanjoinene, $C_{29}H_{35}N_3O_4$, $mp\;281{\sim}2^{\circ}$, $[{\alpha}]^{22}_D-272$, Sanjoinine-D, $C_{32}H_{46}N_4O_5$, $mp\;256{\sim}8^{\circ}$, $[{\alpha}]^{22}_D-53.6$, Sanjoinine-F, $C_{31}H_{42}N_4O_5$, $mp\;228{\sim}9^{\circ}$, $[{\alpha}]^{22}_D-215$, and $Sanjoinine-G_1,\;C_{31}H_{44}N_4O_5,\;mp\;236{\sim}8^{\circ},\;[{\alpha}]^{22}_D-68.6$, were found as 14-membered cyclic peptide alkaloids, $Sanjoinine-G_2,\;C_{30}H_{42}N_4O_4,\;mp\;182^{\circ},\;[{\alpha}]^{22}_D-79.2$, as being open chain peptide alkaloid, and Sanjoinine-E, $C_{19}H_{21}NO_2$, $mp\;166^{\circ}$, $[{\alpha}]^{20}_D-146.2$, N-Methylasimilobine, $C_{18}H_{19}NO_2$, $mp\;193{\sim}5^{\circ}$, $[{\alpha}]^{20}_D-204$, Sanjoinine-Ia, $C_{18}H_{19}NO_2$, $mp\;155{\sim}7^{\circ}$, $[{\alpha}]^{20}_D-140$, Sanjoinine-Ib, $C_{19}H_{21}NO_4$, $mp\;184^{\circ}$, Sanjoinine-K, $C_{16}H_{19}NO_3$, $mp\;159{\sim}61^{\circ}$, $[{\alpha}]^{20}_D+35$, Caaverine, $C_{17}H_{17}NO_2$, $mp\;204^{\circ}$, $[{\alpha}]^{20}_D-80$, and Zizyphusine, $C_{20}H_{24}NO_4$, $mp\;214{\sim}6^{\circ}$, $[{\alpha}]^{20}_D+317$ as being aporphine alkaloids. The heat treatment of the cyclic peptide alkaloids produced their isomeric products which showed enhanced sedative activity. The chemical structure of the isomeric products will be discussed.

• Journal of Aquaculture
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• v.19 no.4
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• pp.236-241
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• 2006

Seasonal variation of the infection by three species of symbiotic copepods in clam, Tresus keenae was investigated in south coast of Korea. The prevalence and abundance of three symbiotic copepods showed seasonal variation with a peak in winter. Reproduction of the copepods occurs from early autumn to spring as indicated by higher ratio of gravid copepods. The frequency distribution of Lichomolgus inflatus, Pseudomyicola spinosus and Anthessius progectus in their host population could be fitted with negative binominal distribution. Results from the present study suggest that seasonal fluctuation in prevalence, relative density and mean intensity is related to temperature and life history of the symbiotic copepods.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.59 no.3
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• pp.242-252
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• 2023

In this study, we aimed to determine the seasonal distribution and biomass of fish in Samcheok marine ranching area (MRA) of Republic of Korea using the scientific echosounder. Fish trap and gillnets were used to identify fish species in the survey area, and dB-difference method was used to estimation the spatio-temporal distribution and density of fish. The results showed that the dominant species in Samcheok marine ranching area were Chelidonichthys spinosus, Sebastes inermis, Hexagrammos otakii and Tribolodon hakonensis. The spatio-temporal distribution of fish showed that fish had a relatively higher distribution at night than during the day. In addition, the density of fish by season was highest at night in July at 34.22 g/m² and lowest in April at 0.42 g/m².

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.59 no.1
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• pp.9-18
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• 2023

Fish samples were collected by a set net from April to December 2020 at the coastal waters off Tongyeong, Korea to determine fish species composition and seasonal variation. A total of 330,675 fish (1,032,578 g) representing 56 species belonging to 39 families were collected during the sampling period. The dominant species were Engraulis japonicus, Trachurus japonicus and Trichiurus japonicus, which accounted for 98.1% of the total number of individuals and 87.5% of the total biomass collected. E. japonicus and Chelidonichthys spinosus appeared every month off the coast of the Tongyeong. Cluster analysis based on the number of individuals of fish were classified into two groups at the dissimilarity level of 57%, and the results of ANOSIM analysis revealed significant differences in community structure among the two groups.

• Korean Journal of Biological Psychiatry
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• v.4 no.1
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• pp.54-59
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• 1997

Objects : Sanjoin, the seeds of Zizyphus vulgaris var. spinosus has been used as the most important hypnotic agent in chinese medicine to treat insomnia. This research was performed in order to examine the effect of betulinic acid and sanjoinine-A which are components of Sanjoin. Method : Sleeping time, sleep recordings of EEG, EMG, serum serotonin level, and locomotor activity were measured in rats which received betulinic acid and sanjoinine-A as sleep induction material extracted from Sanjoin. Results : 1) Groups received betulinic acid, sanjoinine-A, and lorazepam showed increased sleep time than control group with saline. 2) Groups with betulinic acid, sanjoinine-A, lorazepam and saline recorded ${\beta}$-wave in sleep recordings of EEG. In EMG, there was no significant difference among all groups. 3) No significant difference in serum serotonin level among all groups was found. 4) In autonomic activity testing, groups of betulinic acid, sanjoinine-A, and lorazepam showed significantly more decreased in activity than saline group. In comparison of groups of betulinic acid and sanjoinine-A with a group of lorazepam, there was no significant difference. Conclusion : These results suggest that betulinic acid and sanjoinine-A have the sedative effect like lorazepam rather than sleep effect.

• The Korean Journal of Pharmacology
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• v.18 no.1
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• pp.17-22
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• 1982

Zizyphus seed(Zizyphus vulgaris Lamark var. Spinosus Bunge) has long been used as hypnotics and sedatives in oriental medicine, and it is reported that the Zizyphus seed elicited a variety of pharmacologic actions besides CNS depression. Present study was undertaken to investigate the effects of Zizyphus seed on the central nervous system and on the blood pressure. The effect of Zizyphus seed on the central nervous system was measured by the influence of thiopental sleeping time and by inhibition of chemical convulsion (strychnine and pentylenetetrazol induced). Blood pressure changes by Zizyphus extract and its mode of action were investigated. The ground Zizyphus seed was extracted with hexane and methanol, consecutively and the supernatants were discarded. The precipitate was re-extracted with distilled water and the supernatant was evaporated to a dark-brownish sticky liquid, which was used as Zizyphus seed extract in this study after dissolving in saline prior to experiment. The results are as follows. 1) Zizyphus seed extract caused marked prolongation of the thiopental sleeping time in mice. 2) The chemical convulsion by strychnine and pentylenetetrazol, and the mortality by them in chicks were not affected by pretreatment of Zizyphus seed extract. 3) Zizyphus seed extract produced transient fall of blood pressure in the cat, and this hypotentive effect was blocked partially by atropine but not affected by bilateral vagotomy and/or hexamethonium, nor propranolol and, chlorpheniramine and/or cimetidine. With the above results, it may be suggested that the water extract of Zizyphus seeds contains components producing CNS depression and hypotension. Furthermore it is felt that the cholinergic effect, but not the adrenergic or histaminergic, is partly responsible for the hypotensive effect of Zizyphus seed extract.