• 대한기계학회논문집B
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• 제23권10호
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• pp.1265-1273
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• 1999

PIV(Particle Image Velocimetry) velocity field measurements inside the snout of a1/10 scale model of the Zn plating process were carried out at the strip speed $V_s=1.5m/s$. Aluminum powder particles ($1{\mu}m$) and atomized olive oil ($3{\mu}m$) were used as seeding particles to simulate the molten Zinc flow and deoxidization gas flow, respectively. A pulsed Nd:Yag laser and a $2K{\times}2K$ high-resolution CCD camera were synchronized for the PIV velocity field measurement. From flow visualization study, it is found that the liquid flow in the Zn pot is dominantly governed by the uprising flow caused by the rotating sink roll, with its effect on the steel strip inside the snout largely diminished by installing of the snout. The deoxidization gas flow in front of the strip inside the snout can be characterized by a large-scale vortex rotating clockwise direction formed by the moving strip. In the rear side of the strip, a counter-clockwise vortex is formed and some of the flow entrained by the moving strip impinges on the free surface of molten zinc. The liquid flow in front of the strip is governed by the flow entering the snout, caused by the spinning sink roll. Just below the free surface a counter-clockwise vortex is formed near the snout wall. The moving strip affects dominantly the flow behind the strip inside the snout, and large amount of the liquid flow follows the moving strip toward the sink roll. The thickness of the flow following the strip is very thin in the front side due to the uprising flow, however thick boundary layer is formed in the rear side of the strip. Its thickness is increased as moving downstream toward the sink roll. Inside the snout, the deoxidization gas flow above the free surface is much faster than the liquid flow in the zinc pot. Due to the larger influx of the flow following the moving strip in the rear side of the strip, higher percentage of imperfection can be anticipated on the rear surface of the strip.

• 전기화학회지
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• 제4권3호
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• pp.104-108
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• 2001

용융분사법으로 나프탈렌계 메조페이스 피치(mP)를 방사하여 산화안정화 속도를 변화시켜 흑연화 섬유의 모폴러지를 제어하였으며, 흑연화 섬유를 이용하여 Li-ion 이차전지 부극을 제조하여 충$\cdot$방전 거동 및 용량을 측정하였다. 용융분사조건에 따라 제조된 피치섬유의 직경은 $4{\mu}m$로부터 $16{\mu}m$까지 다양하였다 이중에서 직경 $10{\mu}m$인 피치섬유를 선택하여 세가지 승온속도 조건 $2^{\circ}C/min,\;5^{\circ}C/min,\;10^{\circ}/min$에서 산화안정화 후 $1000^{\circ}C$에서 탄소화하여 $2650^{\circ}C$에서 흑연화 한 결과, 섬유 단면이 산화안정화 조건 $2^{\circ}C/min$의 경우는 라디알 구조, $5^{\circ}C/min$의 것은 라디알-랜덤 구조, $10^{\circ}C/min$의 경우는 skin-core 구조를 형성하였고, 승온속도가 큰 경우일수록 이흑연화성이 컷다. 이것은 큰 승온속도에서는 탄소화$\cdot$흑연화 과정에서 섬유표면에서만 산화안정화가 일어나고, 내부에서는 피치분자가 유동성이 커 승온과정에서 고결정성의 흑연구조가 발달한 것으로 추측된다. 따라서 이흑연화성이 큰 $10^{\circ}C/min$에서 산화안정화 한 것이 충전방전 용량이 $2^{\circ}C/min$의 경우에 비해서 1.3배로 약 400mAh/g, 충방전 효율도 $96.8\%$로 가장 우수한 특성을 나타냈다.

• 한국자기학회지
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• 제7권4호
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• pp.173-179
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• 1997

급속응고(melt spinning)법이 Fe-Nd-C의 경우와 마찬가지로 보자력이 높은 Fe-Pr-C 합금을 얻는데에 효과적인지 알아보기 위하여, 급속응고된 Fe-Pr-C 리본에 대하여 냉각속도 및 열처리의 변화에 따른 상, 미세조직 및 자기특성의 변화를 조사하였다. 냉각속도(wheel speed)가 증가할수록 as-spun 리본의 비정질화가 증가하여 40m/s에서 제조된 리본합금은 거의 비정질화하였다. 10m/s에서 제조된 결정질 리본의 상분포는 주소상태의 상분포와 유사하여 .alpha. -Fe가 일차상, Fe$_{17}$Pr$_{2}$C$_{x}$가 이차상으로 존재하였다. 20m/s에서도 결정질이 우세하게 나타나나, 이때에는 .alpha. -Fe의 정출이 약간 억제되는 반면 Fe$_{17}$Pr$_{2}$C$_{x}$의 정출이 현저하였으며, 30m/s에서는 비정질이 우세하여 소량의 결정질만이 존재하였다. 따라서 강자성 Fe$_{14}$Pr$_{2}$C 상은 as-spun 상태에서는 존재하지 않거나 미량이었으며, 주조합금의 경우와 마찬가지로 고상변태를 통해서 얻을 수 있었다. 일반적으로 as-spun리본이 비정질화할수록 비교적 낮은 온도에서 수분의 열처리만으로 완전한 Fe$_{14}$Pr$_{2}$C 상을 얻을 수 있었으며, 결정화가 완벽할수록 Fe$_{14}$Pr$_{2}$C를 얻기 위한 열처리 시간은 증가하였다. 이와 같이 얻은 Fe$_{14}$Pr$_{2}$C는 대부분 1 .mu. m 이하의 미세한 결정립을 가지고 있었으며, as-spun 리본의 비정질화가 완벽한 경우보다 덜 완벽한 경우(30m/s) 또는 결정질과 약간의 비정질이 혼합된 경우 (20m/s)에 열처리에 의한 보자력의 향상이 뚜렷하였다. 일반적으로 변태온도 구역안에서 열처리 온도가 높을수록 10분 이하의 짧은 열처리가 보자력의 향상에 효과적이었다.이었다.

• 한국운동역학회지
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• 제17권1호
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• pp.145-153
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• 2007

The purpose of this study is to compare and analyze the phase-by-phase elapsed time, the COG, the body joint angle changes and the angular velocities of each phase of Handspring Salto Forward Piked performed by 4 college gymnasts through 3D movement analysis program. 1. The average elapsed time for each phase was .13sec for Phase 1, .18sec for Phase 2, .4sec for Phase 3, and .3sec for Phase 5. The elapsed time for Phase 1 to Phase 3 handspring was .35sec on average and the elapsed time for Phase 4 to Phase 5 handspring salto forward piked was .7sec on average. And so it showed that the whole elapsed time was 1.44sec. 2. The average horizontal changes of COG were 93.2 cm at E1, 138. 5 cm at E2, 215.7 cm at E3, 369.2 cm at E4, 450.7 cm at E5, and 553.1 cm at E6. The average vertical changes of COG were 83.1 cm at E1, 71.3 cm at E2, 78.9 cm at E3, 93.7 cm at E4, 150.8 cm at E5, and 97.2 cm at E6. 3. The average shoulder joint angles at each phase were 131.6 deg at E1, 153.5 deg at E2, 135.4 deg at E3, 113.4 deg at E4, 39.6 deg at E5, and 67.5 deg at E6. And the average hip joint angles at each phase were 82.2 deg at E1, 60 deg at E2, 101.9 deg at E3, 161.2 deg at E4, 97.7 deg at E5, and 167 deg at E6. 4. The average shoulder joint angular velocities at each phase were 130.9deg/s E1, 73.1 deg/s at E2, -133.9 deg/s at E3, -194.4 deg/s at E4, 29.4 deg/s at E5, and -50.1 deg/s at E6. And the average hip joint angular velocities at each phase were -154.7 deg/s E1, -96.5 deg/s at E2, 495.9 deg/s at E3, 281.5 deg/s at E4, 90.3 deg/s at E5, and 181.7 deg/s at E6. The results shows that, as for the performance of handspring salto forward piked, it is important to move in short time and horizontally from the hop step to the point to place the hands on the floor and jump, and to stretch the hip joints as much as possible after the displacement of the hands and to keep the hip joints stretched and high in the vertical position at the takeoff. And it is also important to bend the shoulder joints and the hip joints fast and spin as much as possible after the takeoff, and to decrease the speed of spinning by bending he shoulder joints and the hip joints quickly after the highest point of COG and make a stable landing.

• 한국근적외분광분석학회:학술대회논문집
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• 한국근적외분광분석학회 2001년도 NIR-2001
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• pp.1513-1513
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• 2001

Present Food Legislation compels dairy industry to carry out analyses in order to guarantee the food safety and quality of products. Furthermore, in many cases industry pays milk according to bacteriological or/and nutritional quality. In order to do these analyses, several expensive instruments are needed (Milkoscan, Fossomatic, Bactoscan). NIRS technology Provides a unique instrument to deal with all analytical requirements. It offers as main advantages its speed and, specially, its versatility, since not only allows determine all the parameters required in milk analysis, but also allows analyse other dairy products, like cheese or whey. The objective of this study is to develop NIRS calibration equations to predict several quality parameters in goat milk, cheese and whey. Three sets of 123 milk samples, 190 cheese samples and 109 whey samples, have been analysed in a FOSS NIR Systems 6500 I spectrophotometer equipped with a spinning module. Milk and whey were analysed by folded transmission, using circular cells with gold surface and pathlength of 0.1 m, while intact cheese was analysed by reflectance using standard circular cells. NIRS calibrations were obtained for the prediction of chemical composition in goat milk, for fat (r$^2$=0.92; SECV=0.20%), total solids (r$^2$=0.95: SECV=0.22%), protein (r$^2$=0.94; SECV=0.07%), casein (r$^2$=0.93; SECV=0.07%) and lactose (r$^2$=0.89; SECV=0.05%). Moreover, equations have been performed to determine somatic cells (r$^2$=0.81; SECV=276.89%) and total bacteria (r$^2$=0.58; SECV=499.32%) counts in goat milk. In the case of cheese, calibrations were obtained for the prediction of fat (r$^2$=0.92; SECV=0.57), total solids (r$^2$=0.80; SECV=0.92%) and protein (r$^2$=0.70; SECV=0.63%). In whey, fat (r$^2$=0.66; SECV=0.08%), total solids (r$^2$=0.67; SECV=0.19%) and protein (r$^2$=0.76; SECV=0.07%) NIRS equations were obtained. These results proved the viability of NIRS technology to predict chemical and microbiological parameters and somatic cells count in goat milk, as well as chemical composition of goat cheese and whey.

• 한국근적외분광분석학회:학술대회논문집
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• 한국근적외분광분석학회 2001년도 NIR-2001
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• pp.1251-1251
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• 2001

The routine analysis of milk chemical components is of major importance both for the management of animals in dairy farms and for quality control in dairy industries. NIRS technology is an analytical technique which greatly simplifies this routine. One of the most critical aspects in NIRS analysis of milk is sample preparation and analysis modes which should be fast and straightforward. An important difficulty when obtaining NIR spectra of milk is the high water content (80 to 90%) of this product, since water absorbs most of the infrared radiation, and, therefore, limits the accuracy of calibrating for other constituents. To avoid this problem, the DESIR system was set up. Other ways of radiation-sample interaction adapted for liquids or semi-liquids exist, which are practically instantaneous and with limited or null necessity of sample preparation: Transmission and Folded Transmission or Transflectance. The objective of the present work is to compare the precision and accuracy of milk calibration equations in two analysis modes: Reflectance (dry milk) and Folded Transmission (liquid milk). A FOSS-NIR Systems 6500 I spectrophotometer (400-2500 nm) provided with a spinning module was used. Two NIR spectroscopic methods for milk analysis were compared: a) folded transmission: liquid milk samples in a 0.1 pathlength sample cell (ref. IH-0345) and b) reflectance: dried milk samples in glass fibre filters placed in a standard ring cell. A set of 101 milk samples was used to develop the calibration equations, for the two NIR analysis modes, to predict casein, protein, fat and dry matter contents, and 48 milk samples to predict Somatic Cell Count (SCC). The calibrations obtained for protein, fat and dry matter have an excellent quantitative prediction power, since they present $r^2$ values higher than 0.9. The $r^2$ values are slightly lower for casein and SCC (0.88 and 0.89 respectively), but they still are sufficiently high. The accuracy of casein, protein and SCC equations is not affected by the analysis modes, since their ETVC values are very similar in reflectance and folded transmission (0.19% vs 0.21%; 0.16% vs 0.19% and 55.57% vs 53.11% respectively), Lower SECV values were obtained for the prediction of fat and dry matter with the folded transmission equations (0.14% and 0.25% respectively) compared to the results with the reflectance ones (0.43% and 0.34% respectively). In terms of accuracy and speed of analytical response, NIRS analysis of liquid milk is recommended (folded transmission), since the drying procedure takes 24 hours. However, both analysis modes offer satisfactory results.

• 한국근적외분광분석학회:학술대회논문집
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• 한국근적외분광분석학회 2001년도 NIR-2001
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• pp.1615-1615
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• 2001

During the past ten years, Near Infrared Spectroscopy has been successfully applied to the analysis of a great variety of agriculture products. Previous works (Morra et al., 1991; Salgo et al., 1998) have shown the potential of this technology for soil analysis, estimating different parameters just with one single scan. The main advantages of NIR applications in soils are the speed of response, allowing the increase of the number of samples analysed to define a particular soil, and the instantaneous elaboration of recommendations for fertilization and soil amendment. Another advantage is to avoid the use of chemical reagents at all, being an environmentally safe technique. In this paper, we have studied a set of 129 soil samples selected from representative glasshouse soils from Southern Spain. The samples were dried, milled, and sieved to pass a 2 mm sieve and then analysed for organic carbon, total nitrogen, inorganic nitrogen (nitrate ammonium), hygroscopic humidity, pH and electrical conductivity in the 1:1 extract. NIR spectra of all samples were obtained in reflectance mode using a Foss NIR Systems 6500 spectrophotometer equipped with a spinning module. Calibration equations were developed for seven analytical parameters (ph, Total nitrogen, organic nitrogen, organic carbon, C/N ratio and Electric Conductivity). Preliminary results show good correlation coefficients and standard errors of cross validation in equations obtained for Organic Carbon, Organic Nitrogen, Total Nitrogen and C/N ratio. Calibrations for nitrates and nitrites, ammonia and electric conductivity were not acceptable. Calibration obtained for pH had an acceptable SECV, but the determination coefficient was found very poor probably due to the reduced range in reference values. Since the estimation of Organic Carbon and C/N ratio are acceptable NIIRS could be used as a fast method to assess the necessity of organic amendments in soils from Mediterranean regions where the low level of organic matter in soils constitutes an important agronomic problem. Furthermore, the possibility of a single and fast estimation of Total Nitrogen (tedious determination by modifications of the Kjeldahl procedure) could provide and interesting data to use in the estimation of nitrogen fertilizer rates by means of nitrogen balances.

• 한국식품과학회지
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• 제9권2호
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• pp.123-130
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• 1977

동방유량(東邦流糧)에서 공급(供給)받은 저변성(低變成) 탈지대두박(脫脂大豆粕)을 0.02N NaOH액(液)으로 추출(抽出)하여 Soy Protein Isolate (SPI)의 수율(收率)을 증류수나 염용액(鹽溶液)으로 추출(抽出)할때 보다 높이는 과정(過程)(84 % 수율(收率))을 확립(確立)하였으며 제조(製造)된 SPI는 92.1 %의 단백질(蛋白質)을 함유(含有)하였다. SPI를 $70^{\circ}C$에서 moisture heat를 하면 80분간(分間)에 5 %의 변성(變性)이 일어났으나 $95^{\circ}C$에서는 20분(分) 처리(處理)에 의(依)하여 95 %의 변성(變性)이 일어났다. SPI의 alkali 현탄액 (dope solution)은 SPI의 농도(濃度) 15 %, NaOH 농도(濃度) 0.6 %일때 7분(分)만에 60 poises를 나타내고 계속 안정(安定)된 점도(粘度)를 유지하였다. NaOH의 농도(濃度)가 0.9 %일 때는 시간(時間)이 경과(經過)함에 따라 점도(粘度)는 증가(增加)하였다가 감소(減少)하는 경향(傾向)을 보였다. syringe needle (dia. 0.3 mm)로서 모의(模擬) 실험(實驗) 결과(結果) 점도(粘度)가 $28^{\circ}C$에서 60 Poises일때 가장 적합한 단백질섬유(蛋白質纖維)의 texture를 보여주었다. 가열(加熱)에 의(依)한 SPI의 gelation은 8 %이상(以上)의 SPI농도(濃度)일 때 gel이 형성(形成)되기 시작하였으며 $100^{\circ}C$까지 안정(安定)한 gel을 형성(形成)하였고 SPI의 농도(濃度)가 12 %일때 $120^{\circ}C$에서 30분(分) 가열(加熱)에 의(依)하여 20,000 Poises의 좋은 점도(粘度)를 가졌으나 $120^{\circ}C$이상에서는 gel의 continuity가 떨어져 과립상(果粒上)의 gel이 생성(生成)되었다. SPI의 유화력(乳化力)은 pH가 8.7, NaCl의 농도(濃度)가 2 %일때 가장 큰 유화력(乳化力)을 나타냈다. milk casein과 비교(比較) 실험(實驗) 결과(結果) 유화력(乳化力)은 약간 낮았으나 염(鹽)이 존재(存在)하는 경우는 근사한 유화력(乳化力)을 보였다. 그러나 SPI의 foaming capacity는 foaming stability 면(面)에서 egg albumin이나 milk casein과 비교(比較)하여 양호(良好)한 결과(結果)를 보여주었다.

• 한국진공학회:학술대회논문집
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• 한국진공학회 2013년도 제45회 하계 정기학술대회 초록집
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• pp.88-89
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• 2013

A variety of influenza A viruses from animal hosts are continuously prevalent throughout the world which cause human epidemics resulting millions of human infections and enormous industrial and economic damages. Thus, early diagnosis of such pathogen is of paramount importance for biomedical examination and public healthcare screening. To approach this issue, here we propose a fully integrated Rotary genetic analysis system, called Rotary Genetic Analyzer, for on-site detection of influenza A viruses with high speed. The Rotary Genetic Analyzer is made up of four parts including a disposable microchip, a servo motor for precise and high rate spinning of the chip, thermal blocks for temperature control, and a miniaturized optical fluorescence detector as shown Fig. 1. A thermal block made from duralumin is integrated with a film heater at the bottom and a resistance temperature detector (RTD) in the middle. For the efficient performance of RT-PCR, three thermal blocks are placed on the Rotary stage and the temperature of each block is corresponded to the thermal cycling, namely $95^{\circ}C$ (denature), $58^{\circ}C$ (annealing), and $72^{\circ}C$ (extension). Rotary RT-PCR was performed to amplify the target gene which was monitored by an optical fluorescent detector above the extension block. A disposable microdevice (10 cm diameter) consists of a solid-phase extraction based sample pretreatment unit, bead chamber, and 4 ${\mu}L$ of the PCR chamber as shown Fig. 2. The microchip is fabricated using a patterned polycarbonate (PC) sheet with 1 mm thickness and a PC film with 130 ${\mu}m$ thickness, which layers are thermally bonded at $138^{\circ}C$ using acetone vapour. Silicatreated microglass beads with 150~212 ${\mu}L$ diameter are introduced into the sample pretreatment chambers and held in place by weir structure for construction of solid-phase extraction system. Fig. 3 shows strobed images of sequential loading of three samples. Three samples were loaded into the reservoir simultaneously (Fig. 3A), then the influenza A H3N2 viral RNA sample was loaded at 5000 RPM for 10 sec (Fig. 3B). Washing buffer was followed at 5000 RPM for 5 min (Fig. 3C), and angular frequency was decreased to 100 RPM for siphon priming of PCR cocktail to the channel as shown in Figure 3D. Finally the PCR cocktail was loaded to the bead chamber at 2000 RPM for 10 sec, and then RPM was increased up to 5000 RPM for 1 min to obtain the as much as PCR cocktail containing the RNA template (Fig. 3E). In this system, the wastes from RNA samples and washing buffer were transported to the waste chamber, which is fully filled to the chamber with precise optimization. Then, the PCR cocktail was able to transport to the PCR chamber. Fig. 3F shows the final image of the sample pretreatment. PCR cocktail containing RNA template is successfully isolated from waste. To detect the influenza A H3N2 virus, the purified RNA with PCR cocktail in the PCR chamber was amplified by using performed the RNA capture on the proposed microdevice. The fluorescence images were described in Figure 4A at the 0, 40 cycles. The fluorescence signal (40 cycle) was drastically increased confirming the influenza A H3N2 virus. The real-time profiles were successfully obtained using the optical fluorescence detector as shown in Figure 4B. The Rotary PCR and off-chip PCR were compared with same amount of influenza A H3N2 virus. The Ct value of Rotary PCR was smaller than the off-chip PCR without contamination. The whole process of the sample pretreatment and RT-PCR could be accomplished in 30 min on the fully integrated Rotary Genetic Analyzer system. We have demonstrated a fully integrated and portable Rotary Genetic Analyzer for detection of the gene expression of influenza A virus, which has 'Sample-in-answer-out' capability including sample pretreatment, rotary amplification, and optical detection. Target gene amplification was real-time monitored using the integrated Rotary Genetic Analyzer system.