• Development and Reproduction
• /
• v.18 no.2
• /
• pp.117-125
• /
• 2014

Vitrification method is widely used in oocyte cryopreservation for IVF but the birth rates are lower than that of the fresh oocyte. One of the known main reasons is structural instability of meiotic spindle and chromosome systems of mature oocyte. To get the best way for keeping competence of matured oocytes, we studied the best conditions for vitrification focused on equilibration times. The mature oocytes were underwent vitrification with current popular method and analyzed the survival rates, microtubule stability and DNA integrity. The survival rates of recovered oocyte are almost same between groups and are more than 93%. The structural configuration of meiotic spindle was well kept in 10 min equilibration group and the stability rate was almost same with that of control. The chromosomal breakdown was observed in all experimental groups, but the chromosomal stability was higher in 10 min equilibration group than the other groups. The 10 min equilibration group showed best condition compared with the other groups. Based on these results, the equilibration time is one of the key factors in successful keeping for competence of mature oocyte. Although, more fine analysis about the effects of physical stress on oocyte during vitrification is needed to define the optimal condition, it is suggested that the optimal equilibration time to get competent oocyte in mouse is 10 min. Information acquired this study may provide insight into intracellular structural events occurring in human oocytes after vitrification and application for cryopreservation of human oocyte.

• KSTLE International Journal
• /
• v.3 no.2
• /
• pp.79-83
• /
• 2002

Sealing an oil-air mixture plays important roles to have an enhanced lubrication for high speed spindle. High speed spindle requires non-contact type of sealing mechanism. Current work emphases on the investigation of the air jet effect on the protective collar type labyrinth seal. To improve the sealing capability of conventional labyrinth seal, air jet was injected against through the leakage flow, It has a combined geometry of a protective collar type and an air jet type. Both of a numerical analysis by CFD (Computational Fluid Dynamics) and experimental measurements are carried out to verify sealing improvement The sealing effects of the leakage clearance and the air jet magnitude are studied in various parameters. The results of pressure drop in the experiment match reasonably to those of the simulation by introducing a flow coefficient Effect of sealing improvement is explained as decreasing of leakage clearance by air jetting. Thus, sealing effect is improved by amount of air jetting even though clearance becomes larger.

• Proceedings of the Korean Society of Precision Engineering Conference
• /
• 2000.06a
• /
• pp.61-70
• /
• 2000

This paper described the current status of machine tool technology and its future trends with a particular emphasis on high-speed machining. People in machine tool industry have continuously sought to serve fast-changing manufacturing industry with economical machining solutins. At presents, it appears that more productivity gain is demanded to shorten time-to-market and machining requirements become more stringent. In this regard, this paper firstly addressed a high-speed spindle as a key element for the next-generation machine tools. The sequel to it apparently went to high-speed feed axes and final discussion included the problem of how to optimize overall system including servo function. Lastly a brief look to NC technology including machine intelligence was taken.

• Proceedings of the Korean Society of Precision Engineering Conference
• /
• 2000.05a
• /
• pp.708-713
• /
• 2000

A research on the AFC(Auto Feedrate Control) by a fuzzy controller using a tool dynamometer and motor currents was conducted. For simulations, cutting dynamics of end-milling process was modeled by geometric relationship between tool and work-piece. The fuzzy logic controller was employed to track the desired cutting force and showed good performance in simulations and several experiments. The spindle motor currents was modeled to estimate cutting force and successfully used for the AFC.

• Proceedings of the KIEE Conference
• /
• 2005.07b
• /
• pp.1114-1116
• /
• 2005

This paper introduces an electric gear to run a high speed spindle motor. Compared with inverter driving, electric gear can be used to reduce switching loss caused by using high switching frequency and improve heating problem on reactor with sinusoidal current wave form. In this paper advantages and disadvantages of electric gear are presented in comparison with those of inverter driving and mechanical gear, and its usefulness is verified by means of experimental approach.

• Journal of the Korean Wood Science and Technology
• /
• v.21 no.2
• /
• pp.23-30
• /
• 1993

As the demand of wood furniture becomes diverse and large-sized, artificial intelligence technique is required to design the expert system which can promote the efficiency of the operations management in wood furniture industry. This study was carried out to develop the expert scheduler, which was applied to the scheduling in chair-manufacturing process to evaluate its validity. The expert scheduler could show the results of scheduling must faster than Gantt chart method with ease. Maximum tardiness in the current chair-manufacturing process could be reduced from 29 seconds to 5 seconds by the addition of a spindle sander, a 12 spindle universal boring machine, and a moulding sander to sanding, boring, and moulding process, respectively.

• Journal of the Korean Society for Precision Engineering
• /
• v.19 no.10
• /
• pp.107-113
• /
• 2002

To monitor the torque of an induction motor using current, rotating speed has been measured and used to calculate the slip angular velocity. Additional sensor, however, can cause extra expense and trouble. In this paper, a new algorithm is proposed to monitor the torque of vector controlled induction motor without any speed measuring sensor. Only stator current is measured to estimate the magnetizing current which is used to calculate flux linkage, rotor velocity and motor torque. Graphical programming is used to implement the suggested algorithm and to monitor the torque of an induction motor in real time. To solve the fluctuation problem of estimated torque caused from instantly varying rotating speed of an induction motor, the rotating speed is reconstructed based on the measured current signals. From several experiments, the proposed method shows a good estimation of the motor torque under the normal rotational speed.

• Journal of the Korean Society for Precision Engineering
• /
• v.12 no.2
• /
• pp.23-34
• /
• 1995

This paper is concerned with effective and reliable tool breakage detection method using pattern characteristics of feed motor current in milling operations. Correlation coefficient is derived from the feature vector of signal for two consecutive which are extracted feed motor current over three spindle revolutions. The changing pattern of correlation coefficient is continuously compared to detect tool breakage and monitor cutting conditions. This proposed monitoring scheme is not affected by different tools, friction of motion, and varying cutting conditions and material shapes. Experimental results are presented to support the proposed monitoring scheme.

• Journal of the Korean Society of Manufacturing Process Engineers
• /
• v.14 no.6
• /
• pp.1-6
• /
• 2015

Tool condition monitoring plays one of the most important roles in the improvement of both machining quality and productivity. In this regard, various process signals and monitoring methods have been developed. However, most of the existing studies used cutting force or acoustic emission signals, which posed risks of interference with the machining system in dynamics, fixturing, and machining configuration. In this study, a feed motor current signal is used as a process signal representing process and tool states in tool breakage monitoring based on an adaptive autoregressive model and unsupervised neural network. From the experimental results using various cases of tool breakage, it is shown that the developed system can successfully detect tool breakage before two revolutions of the spindle after tool breakage.

• Proceedings of the Korean Society of Precision Engineering Conference
• /
• 2002.05a
• /
• pp.211-214
• /
• 2002

The detection of tool wear is very important in an automated manufacturing system. This paper presents a tool condition monitoring system based on the wavelet transform analysis of the AC servo motor current in a milling process. The current measurement is relatively simple and does not affect machining operations. The discrete wavelet transform was used to decompose the current of a spindle AC servo motor in the time and frequency domain. The feature vectors were extracted from the decomposed signals and compared to clarity normal and wear conditions. The results show the feasibility of the wavelet transform analysis for the tool condition monitoring.